RESUMEN
Thymocyte antigen-1 (THY-1)is a potential target for rheumatoid arthritis (RA) treatment, and THY-1 positive fibroblast-like synoviocytes (FLS) are enriched in the synovium of RA patients and participate in angiogenesis to accelerate RA progression. In this study, we screened an antibody targeting THY-1 (THY-1 Ab) and explored its mechanism in alleviating RA progression. THY-1 Ab was screened from ScFv phage antibody library by phage display technology (PDT). THY-1 Ab-treated collagen induced arthritis (CIA) mice had lower degree of arthritis scores. We explore the mechanism of THY-1 Ab in alleviating RA progression. THY-1 Ab can remarkably inhibit the secretion of pro-inflammatory factors and promote the secretion of anti-inflammatory factors. Further experiments showed that THY1 Ab downregulated the expression of JUNB by the hsa_circ_0094342/miRNA-155-5P/SPI1 axis, inhibited RA angiogenesis and osteoclast differentiation, and relieved RA progression. These findings support that THY-1 Ab is a promising therapeutic antibody for RA treatment.
Asunto(s)
Artritis Experimental , Artritis Reumatoide , MicroARNs , Animales , Humanos , Ratones , Artritis Experimental/terapia , Artritis Experimental/metabolismo , Artritis Reumatoide/terapia , Artritis Reumatoide/metabolismo , Proliferación Celular , Células Cultivadas , Fibroblastos/metabolismo , Inmunoterapia , MicroARNs/genética , MicroARNs/metabolismo , Osteoclastos/metabolismo , Membrana Sinovial/metabolismo , Timocitos/metabolismo , Antígenos/inmunologíaRESUMEN
A number of studies have found a concreteness effect and an abstractness effect in word processing. The triple-component hypothesis proposed that the emotional aspect of words, such as the valence of words, contributes to the abstractness effect. Although there is no direct evidence for the role of affective characteristics of individuals in the abstractness effect, some studies have found a negative bias of highly neurotic individuals. The current study was designed to examine the abstractness effect of words for individuals who were highly neurotic. The event-related potential results showed that highly neurotic individuals exhibited an abstractness effect for negative words on the P300, which was evoked by emotion information, and a concreteness effect for negative words on the N400, which was activated by semantic processing. These results are discussed from the perspective of triple-component hypothesis.