Mainstream short-cut N removal modelling: current status and perspectives.

This work gives an overview of the state-of-the-art in modelling of short-cut processes for nitrogen removal in mainstream wastewater treatment and presents future perspectives for directing research efforts in line with the needs of practice. The modelling status for deammonification (i.e., anammox-based) and nitrite-shunt processes is presented with its challenges and limitations. The importance of mathematical models for considering N2O emissions in the design and operation of short-cut nitrogen removal processes is considered as well. Modelling goals and potential benefits are presented and the needs for new and more advanced approaches are identified. Overall, this contribution presents how existing and future mathematical models can accelerate successful full-scale mainstream short-cut nitrogen removal applications.

Robust Nitritation Sustained by Acid-Tolerant Ammonia-Oxidizing Bacteria.

Oxidation of ammonium to nitrite rather than nitrate, i.e., nitritation, is critical for autotrophic nitrogen removal. This study demonstrates a robust nitritation process in treating low-strength wastewater, obtained from a mixture of real mainstream sewage with sidestream anaerobic digestion liquor. This is achieved through cultivating acid-tolerant ammonia-oxidizing bacteria (AOB) in a laboratory nitrifying bioreactor at pH 4.5-5.0. It was shown that nitrite accumulation with a high NO2-/(NO2- + NO3-) ratio of 95 ± 5% was stably maintained for more than 300 days, and the obtained volumetric NH4+ removal rate (i.e., 188 ± 14 mg N L-1 d-1) was practically useful. 16S rRNA gene sequencing analyses indicated the dominance of new AOB, "Candidatus Nitrosoglobus," in the nitrifying guild (i.e., 1.90 ± 0.08% in the total community), with the disappearance of typical activated sludge nitrifying microorganisms, including Nitrosomonas, Nitrospira, and Nitrobacter. This is the first identification of Ca. Nitrosoglobus as key ammonia oxidizers in a wastewater treatment system. It was found that Ca. Nitrosoglobus can tolerate low pH (<5.0), and free nitrous acid (FNA) at levels that inhibit AOB and nitrite-oxidizing bacteria (NOB) commonly found in wastewater treatment processes. The in situ inhibition of NOB leads to accumulation of nitrite (NO2-), which along with protons (H+) also produced in ammonium oxidation generates and sustains FNA at 3.0 ± 1.4 mg HNO2-N L-1. As such, robust PN was achieved under acidic conditions, with a complete absence of NOB. Compared to previous nitritation systems, this acidic nitritation process is featured by a higher nitric oxide (NO) but a lower nitrous oxide (N2O) emission level, with the emission factors estimated at 1.57 ± 0.08 and 0.57 ± 0.03%, respectively, of influent ammonium nitrogen load.

Impacts of feed dilution and lower solids retention time on performance of thermal hydrolysis/anaerobic digestion.

The goal of this study was to evaluate using feed dilution/solids retention time (SRT) control to manage potential ammonia inhibition in highly loaded anaerobic digesters after thermal hydrolysis. The study compared three digesters operated at the same target volatile solids (VS) loading rate of 5.5 kg VS/d-m3 , but at different feed concentrations resulting in SRTs of 10, 15, and 18 days. Lowering the feed concentration decreased the digester total ammonia nitrogen concentrations which averaged 1,580, 2,610, and 3,080 mg NH 4 + -N/L for the 10-, 15-, and 18-day digesters. The VS reduction and methane yields were equivalent for the 15- and 18-day digesters and about 4% lower for the 10-day digester. Ammonia inhibition of the 18-day digester occurred early in the study, but the system acclimated over time. Feed dilution reduced the viscosity and the potential for volume expansion due to gas holdup and foaming. PRACTIONER POINTS: Feed dilution reduces digester ammonia concentrations and inhibition potential without sacrificing digester performance at lower SRTs. Feed dilution greatly reduces digester viscosity and associated issues with digester volume expansion due to gas holdup and foaming. Operating at the lower SRT does not impact cake solids after dewatering and substantially decreases polymer demand for conditioning.

Impact of carbon source and COD/N on the concurrent operation of partial denitrification and anammox.

In this study, concurrent operation of anammox and partial denitrification within a nonacclimated mixed culture system was proposed. The impact of carbon sources (acetate, glycerol, methanol, and ethanol) and COD/NO3- -N ratio on partial denitrification selection under both short- and long-term operations was investigated. Results from short-term testing showed that all carbon sources supported partial denitrification. However, acetate and glycerol were preferred due to their display of efficient partial denitrification selection, which may be related to their different electron transport pathways in comparison with methanol. Long-term operation confirmed results of batch tests by showing the contribution of partial denitrification to nitrate removal above 90% after acclimation in both acetate and glycerol reactors. In contrast, methanol showed challenges of maintaining efficient partial denitrification. COD/NO3- -N ratio mainly controlled the rate of nitrate reduction and not directly partial denitrification selection; thus, it should be used to balance between denitrification rate and anammox rate. PRACTITIONER POINTS: The authors aimed to investigate the impact of carbon sources and COD/NO3-N ratio on partial denitrification selection. All the carbon sources supported partial denitrification as long as the nitrite sink was available. 90% partial denitrification could be achieved with both acetate and glycerol in long-term operations. COD/NO3-N ratio did not directly control partial denitrification but can be used to balance between denitrification rate and anammox rate.

Colloids, flocculation and carbon capture - a comprehensive plant-wide model.

The implementation of carbon capture technologies such as high-rate activated sludge (HRAS) systems are gaining interests in water resource and recovery facilities (WRRFs) to minimize carbon oxidation and maximize organic carbon recovery and methane potential through biosorption of biodegradable organics into the biomass. Existing activated sludge models were developed to describe chemical oxygen demand (COD) removal in activated sludge systems operating at long solids retention times (SRT) (i.e. 3 days or longer) and fail to simulate the biological reactions at low SRT systems. A new model is developed to describe colloidal material removal and extracellular polymeric substance (EPS) generation, flocculation, and intracellular storage with the objective of extending the range of whole plant models to very short SRT systems. In this study, the model is tested against A-stage (adsorption) pilot reactor performance data and proved to match the COD and colloids removal at low SRT. The model was also tested on longer SRT systems where effluents do not contain much residual colloids, and digestion where colloids from decay processes are present.

Novel Stokesian Metrics that Quantify Collision Efficiency, Floc Strength, and Discrete Settling Behavior.

Novel parameters were developed to predict the effluent quality and settling behavior in clarifiers that cannot conventionally be achieved using either the conventional flux theory or overflow rates. Simple batch experiments based on the critical settling velocity (CSV) selection were used as the basis for the development of three novel parameters: intrinsic settling classes (ISC), threshold of flocculation/flocculation limitation (TOF/α), and floc strength. ISC was proven to accurately (±2%) determine the granule fraction and discrete particle distribution. TOF quantified the minimum solids concentration needed to form large flocs and was directly linked to collision efficiency. In hybrid systems, an exponential fitting on a CSV matrix was proposed to quantify the collision efficiency of flocs (α). Shear studies were conducted to quantify floc strength. The methods were applied to a wide spectrum of sludge types to show the broad applicability and sensitivity of the novel methods.

Assessment of the endogenous respiration rate and the observed biomass yield for methanol-fed denitrifying bacteria under anoxic and aerobic conditions.

In this study, the endogenous respiration rate and the observed biomass yield of denitrifying methylotrophic biomass were estimated through measuring changes in denitrification rates (DNR) as a result of maintaining the biomass under methanol deprived conditions. For this purpose, activated sludge biomass from a full-scale wastewater treatment plant was kept in 10-L batch reactors for 8 days under fully aerobic and anoxic conditions at 20 °C without methanol addition. To investigate temperature effects, another biomass sample was placed under starvation conditions over a period of 10 days under aerobic conditions at 25 °C. A series of secondary batch tests were conducted to measure DNR and observed biomass yields. The decline in DNR over the starvation period was used as a surrogate to biomass decay rate in order to infer the endogenous respiration rates of the methylotrophs. The regression analysis on the declining DNR data shows 95% confidence intervals of 0.130 ± 0.017 day-1 for endogenous respiration rate under aerobic conditions at 20 °C, 0.102 ± 0.013 day-1 under anoxic conditions at 20 °C, and 0.214 ± 0.044 day-1 under aerobic conditions at 25 °C. Results indicated that the endogenous respiration rate of methylotrophs is 20% slower under anoxic conditions than under aerobic conditions, and there is a significant temperature dependency, with an Arrhenius coefficient of 1.10. The observed biomass yield value showed an increasing trend from approximately 0.2 to 0.6 when the starvation time increased from 0 to 10 days.

Reverse flexing as a physical/mechanical treatment to mitigate fouling of fine bubble diffusers.

Achieving energy neutrality has shifted focus towards aeration system optimization, due to the high energy consumption of aeration processes in modern advanced wastewater treatment plants. A study on fine bubble diffuser fouling and mitigation, quantified by dynamic wet pressure (DWP), oxygen transfer efficiency and alpha was carried out in Blue Plains, Washington, DC. Four polyurethane fine bubble diffusers were installed in a pilot reactor column fed with high rate activated sludge from a full scale system. A mechanical cleaning method, reverse flexing (RF), was used to treat two diffusers (RF1, RF2), while two diffusers were kept as a control (i.e., no reverse flexing). There was a 45% increase in DWP of the control diffuser after 17 months of operation, an indication of fouling. RF treated diffusers (RF1 and RF2) did not show significant increase in DWP, and in comparison to the control diffuser prevented about 35% increase in DWP. Hence, reverse flexing potentially saves blower energy, by reducing the pressure burden on the air blower which increases blower energy requirement. However, no significant impact of the RF treatment in preventing a decrease in alpha-fouling (αF) of the fine pore diffusers, over time in operation was observed.

Deammonification for digester supernatant pretreated with thermal hydrolysis: overcoming inhibition through process optimization.

The thermal hydrolysis process (THP) has been proven to be an excellent pretreatment step for an anaerobic digester (AD), increasing biogas yield and decreasing sludge disposal. The goal of this work was to optimize deammonification for efficient nitrogen removal despite the inhibition effects caused by the organics present in the THP-AD sludge filtrate (digestate). Two sequencing batch reactors were studied treating conventional digestate and THP-AD digestate, respectively. Improved process control based on higher dissolved oxygen set-point (1 mg O2/L) and longer aeration times could achieve successful treatment of THP-AD digestate. This increased set-point could overcome the inhibition effect on aerobic ammonium-oxidizing bacteria (AerAOB), potentially caused by particulate and colloidal organics. Moreover, based on the mass balance, anoxic ammonium-oxidizing bacteria (AnAOB) contribution to the total nitrogen removal decreased from 97 ± 1 % for conventional to 72 ± 5 % for THP-AD digestate treatment, but remained stable by selective AnAOB retention using a vibrating screen. Overall, similar total nitrogen removal rates of 520 ± 28 mg N/L/day at a loading rate of 600 mg N/L/day were achieved in the THP-AD reactor compared to the conventional digestate treatment operating at low dissolved oxygen (DO) (0.38 ± 0.10 mg O2/L).

Model-based evaluation of mechanisms and benefits of mainstream shortcut nitrogen removal processes.

The main challenge in implementing shortcut nitrogen removal processes for mainstream wastewater treatment is the out-selection of nitrite oxidizing bacteria (NOB) to limit nitrate production. A model-based approach was utilized to simulate the impact of individual features of process control strategies to achieve NO(-)(2)-N shunt via NOB out-selection. Simulations were conducted using a two-step nitrogen removal model from the literature. Nitrogen shortcut removal processes from two case studies were modeled to illustrate the contribution of NOB out-selection mechanisms. The paper highlights a comparison between two control schemes; one was based on online measured ammonia and the other was based on a target ratio of 1 for ammonia vs. NOx (nitrate + nitrite) (AVN). Results indicated that the AVN controller possesses unique features to nitrify only that amount of nitrogen that can be denitrified, which promotes better management of incoming organics and bicarbonate for a more efficient NOB out-selection. Finally, the model was used in a scenario analysis, simulating hypothetical optimized performance of the pilot process. An estimated potential saving of 60% in carbon addition for nitrogen removal by implementing full-scale mainstream deammonification was predicted.

Case study: Bioaugmenting the comammox dominated biomass from B-stage to enhance nitrification in A-stage at Blue Plains AWWTP.

A comprehensive case study was undertaken at the Blue Plains wastewater treatment plant (WWTP) to explore the bioaugmentation technique of introducing nitrifying sludge into the non-nitrifying stage over the course of two operational years. This innovative approach involved the return of waste activated sludge (WAS) from the biological nutrient removal (BNR) system to enhance the nitrification in the high carbon removal rate system. The complete ammonia oxidizer (comammox) Nitrospira Nitrosa was identified as the main nitrifier in the system. Bioaugmentation was shown to be successful as nitrifiers returned from BNR were able to increase the nitrifying activity of the high carbon removal rate system. There was a positive correlation between returned sludge from the BNR stage and the specific total kjeldahl nitrogen (TKN) removal rate in A stage. The bioaugmentation process resulted in a remarkable threefold increase in the specific TKN removal rate within the A stage. Result suggested that recycling of WAS is a simple technique to bio-augment a low SRT system with nitrifiers and add ammonia oxidation to a previously non-nitrifying stage. The results from this case study hold the potential for applicable implications for other WWTPs that have a similar operational scheme to Blue Plains, allowing them to reuse WAS from the B stage, previously considered waste, to enhance nitrification and thus improving overall nitrogen removal performance. PRACTITIONER POINTS: Comammox identifying as main nitrifier in the B stage. Comammox enriched sludge from B stage successfully bio-augmented the East side of A stage up to threefold. Bioaugmentation of comammox in the West side of A stage was potentially inhibited by the gravity thickened overflow. Sludge returned from B stage to A stage can improve nitrification with a very minor retrofits and short startup times.

Comparing methanol and glycerol as carbon sources for mainstream partial denitrification/anammox in an IFAS process.

This study explored the implementation of mainstream partial denitrification with anammox (PdNA) in the second anoxic zone of a wastewater treatment process in an integrated fixed film activated sludge (IFAS) configuration. A pilot study was conducted to compare the use of methanol and glycerol as external carbon sources for an IFAS PdNA startup, with a goal to optimize nitrogen removal while minimizing carbon usage. The study also investigated the establishment of anammox bacteria on virgin carriers in IFAS reactors without the use of seeding, and it is the first IFAS PdNA startup to use methanol as an external carbon source. The establishment of anammox bacteria was confirmed in both reactors 102 days after startup. Although the glycerol-fed reactor achieved a higher steady-state maximum ammonia removal rate because of anammox bacteria (1.6 ± 0.3 g/m2/day) in comparison with the methanol-fed reactor (1.2 ± 0.2 g/m2/day), both the glycerol- and methanol-fed reactors achieved similar average in situ ammonia removal rates of 0.39 ± 0.2 g/m2/day and 0.40 ± 0.2 g/m2/day, respectively. Additionally, when the upstream ammonia versus NOx (AvN) control system maintained an ideal ratio of 0.40-0.50 g/g, the methanol-fed reactor attained a lower average effluent TIN concentration (3.50 ± 1.2 mg/L) than the glycerol-fed reactor (4.43 ± 1.6 mg/L), which was prone to elevated nitrite concentrations in the effluent. Overall, this research highlights the potential for PdNA in IFAS configurations as an efficient and cost-saving method for wastewater treatment, with methanol as a viable carbon source for the establishment of anammox bacteria. PRACTITIONER POINTS: Methanol is an effective external carbon source for an anammox startup that avoids the need for costly alternative carbon sources. The methanol-fed reactor demonstrated higher TIN removal compared with the glycerol-fed reactor because of less overproduction of nitrite. Anammox bacteria was established in an IFAS reactor without seeding and used internally stored carbon to reduce external carbon addition. Controlling the influent ammonia versus NOx (AvN) ratio between 0.40 and 0.50 g/g allowed for low and stable TIN effluent conditions.

Improving nutrients ratio in class A biosolids through vivianite recovery: Insights from a wastewater resource recovery facility.

Class A biosolids from water resource recovery facilities (WRRFs) are increasingly used as sustainable alternatives to synthetic fertilizers. However, the high phosphorus to nitrogen ratio in biosolids leads to a potential accumulation of phosphorus after repeated land applications. Extracting vivianite, an FeP mineral, prior to the final dewatering step in the biosolids treatment can reduce the P content in the resulting class A biosolids and achieve a P:N ratio closer to the 1:2 of synthetic fertilizers. Using ICP-MS, IC, UV-Vis colorimetric methods, Mössbauer spectroscopy, and SEM-EDX, a full-scale characterization of vivianite at the Blue Plains Advanced Wastewater Treatment Plant (AWTTP) was surveyed throughout the biosolids treatment train. Results showed that the vivianite-bound phosphorus in primary sludge thickening, before pre-dewatering, after thermal hydrolysis, and after anaerobic digestion corresponded to 8 %, 52 %, 40 %, and 49 % of the total phosphorus in the treatment influent. Similarly, the vivianite-bound iron concentration also corresponded to 8 %, 52 %, 40 %, and 49 % of the total iron present (from FeCl3 dosing), because the molar ratio between total iron and total incoming phosphorus was 1.5:1, which is the same stoichiometry of vivianite. Based on current P:N levels in the Class A biosolids at Blue Plains, a vivianite recovery target of 40 % to ideally 70 % is required in locations with high vivianite content to reach a P:N ratio in the resulting class A biosolid that matches synthetic fertilizers of 1:1.3 to 1:2, respectively. A financial analysis on recycling iron from the recovered vivianite had estimated that 14-25 % of Blue Plain's annual FeCl3 demand can potentially be met. Additionally, model simulations with Visual Minteq were used to evaluate the pre-treatment options that maximize vivianite recovery at different solids treatment train locations.

One-stage partial nitritation/anammox at 15 °C on pretreated sewage: feasibility demonstration at lab-scale.

Energy-positive sewage treatment can be achieved by implementation of oxygen-limited autotrophic nitrification/denitrification (OLAND) in the main water line, as the latter does not require organic carbon and therefore allows maximum energy recovery through anaerobic digestion of organics. To test the feasibility of mainstream OLAND, the effect of a gradual temperature decrease from 29 to 15 °C and a chemical oxygen demand (COD)/N increase from 0 to 2 was tested in an OLAND rotating biological contactor operating at 55-60 mg NH4(+)-N L(-1) and a hydraulic retention time of 1 h. Moreover, the effect of the operational conditions and feeding strategies on the reactor cycle balances, including NO and N2O emissions were studied in detail. This study showed for the first time that total nitrogen removal rates of 0.5 g N L(-1) day(-1) can be maintained when decreasing the temperature from 29 to 15 °C and when low nitrogen concentration and moderate COD levels are treated. Nitrite accumulation together with elevated NO and N2O emissions (5% of N load) were needed to favor anammox compared with nitratation at low free ammonia (<0.25 mg N L(-1)), low free nitrous acid (<0.9 µg N L(-1)), and higher DO levels (3-4 mg O2 L(-1)). Although the total nitrogen removal rates showed potential, the accumulation of nitrite and nitrate resulted in lower nitrogen removal efficiencies (around 40%), which should be improved in the future. Moreover, a balance should be found in the future between the increased NO and N2O emissions and a decreased energy consumption to justify OLAND mainstream treatment.

Feasibility of packed-bed trickling filters for partial nitritation/anammox: Effects of carrier material, bottom ventilation openings, hydraulic loading rate and free ammonia.

This study pioneers the feasibility of cost-effective partial nitritation/anammox (PN/A) in packed-bed trickling filters (TFs). Three parallel TFs tested different carrier materials, the presence or absence of bottom ventilation openings, hydraulic loading rates (HLR, 0.4-2.2 m3 m-2 h-1), and free ammonia (FA) levels on synthetic medium. The inexpensive Argex expanded clay was recommended due to the similar nitrogen removal rates as commercially used plastics. Top-only ventilation at an optimum HLR of 1.8 m3 m-2 h-1 could remove approximately 60% of the total nitrogen load (i.e., 300 mg N L-1 d-1, 30 °C) and achieve relatively low NO3--N accumulation (13%). Likely FA levels of around 1.3-3.2 mg N L-1 suppressed nitratation. Most of the total nitrogen removal took place in the upper third of the reactor, where anammox activity was highest. Provided further optimizations, the results demonstrated TFs are suitable for low-energy shortcut nitrogen removal.

Impact of instrumentation reliability on mainstream suspended partial denitrification-anammox (PdNA).

This study successfully revealed the importance of probe reliability and sensitivity with ion sensitive electrode (ISE) probes on achieving high partial denitrification (PdN) efficiency; and decreasing carbon overdosing events that cause the decline of microbial populations and performance of PdNA. In a mainstream integrated hybrid granule-floc system, an average PdN efficiency of 76% was achieved with acetate as the carbon source. Thauera was identified as the dominant PdN species; its presence in the system was analogous to instrumentation reliability and PdN selection and was not a consequence of bioaugmentation. Up to 27-121 mg total inorganic nitrogen/L/d, an equivalent of 18-48% of the overall total inorganic nitrogen removed, was achieved through the PdNA pathway. Candidatus Brocadia was the main anoxic ammonium oxidizing bacteria species that was seeded from sidestream and enriched and retained in the mainstream system with observed growth rates of 0.04-0.13 day-1 . Moreover, there was no direct negative impact of methanol's use for post-polishing on anoxic ammonium oxidizing bacteria activity and growth. PRACTITIONER POINTS: Stress testing with ISE sensors revealed the importance of probe reliability and sensitivity on PdN selection and PdNA performance. Up to 121 mg TIN/L/d was achieved via PdNA in a mainstream suspended hybrid granule-floc partial denitrification-anammox (PdNA) system. Candidatus Brocadia was the dominant AnAOB species with observed growth rates of 0.04-0.13 day-1. There was no direct negative impact of methanol's use for post-polishing on AnAOB activity and growth.

Low diversity and microdiversity of comammox bacteria in wastewater systems suggest specific adaptations within the Ca. Nitrospira nitrosa cluster.

Studies have found Ca. Nitrospira nitrosa-like bacteria to be the principal or sole comammox bacteria in nitrogen removal systems for wastewater treatment. In contrast, multiple populations of strict ammonia and nitrite oxidizers co-exist in similar systems. This apparent lack of diversity is surprising and could impact the feasibility of leveraging comammox bacteria for nitrogen removal. We used full-length 16S rRNA gene sequencing and genome-resolved metagenomics to compare the species-level diversity of comammox bacteria with that of strict nitrifiers in full-scale wastewater treatment systems and assess whether this comparison is consistent or diverged at the strain-level. Full-length 16S rRNA gene sequencing indicated that Nitrosomonas-like bacteria exhibited higher species-level diversity in comparison with other nitrifying bacteria, while the strain-level diversity (also called microdiversity) of most Nitrospira-like bacteria were higher than Nitrosomonas-like bacteria with few exceptions (one Nitrospira lineage II population). Comammox bacterial metagenome assembled genomes (MAGs) were associated with Ca. Nitrospira nitrosa. The average amino acid identity between principal comammox bacterial MAGs (93% ± 3) across systems was significantly higher than that of the Nitrosomonas-like ammonia oxidizers (73% ± 8), the Nitrospira_A-like nitrite oxidizer (85% ± 4), and the Nitrospira_D-like nitrite oxidizer (83% ± 1). This demonstrated the low species-level diversity of comammox bacteria compared with strict nitrifiers and further suggests that the same comammox population was detected in all systems. Comammox bacteria (Nitrospira lineage II), Nitrosomonas and, Nitrospira_D (Nitrospira lineage II) MAGs were significantly less microdiverse than the Nitrospira_A (lineage I) MAGs. Interestingly, strain-resolved analysis also indicates that different nitrogen removal systems harbor different comammox bacterial strains within the Ca. Nitrospira nitrosa cluster. These results suggest that comammox bacteria associated with Ca. Nitrospira nitrosa have low species- and strain-level diversity in nitrogen removal systems and may thus harbor specific adaptations to the wastewater ecosystem.

Efficient total nitrogen removal in an ammonia gas biofilter through high-rate OLAND.

Ammonia gas is conventionally treated in nitrifying biofilters; however, addition of organic carbon to perform post-denitrification is required to obtain total nitrogen removal. Oxygen-limited autotrophic nitrification/denitrification (OLAND), applied in full-scale for wastewater treatment, can offer a cost-effective alternative for gas treatment. In this study, the OLAND application thus was broadened toward ammonia loaded gaseous streams. A down flow, oxygen-saturated biofilter (height of 1.5 m; diameter of 0.11 m) was fed with an ammonia gas stream (248 ± 10 ppmv) at a loading rate of 0.86 ± 0.04 kg N m(-3) biofilter d(-1) and an empty bed residence time of 14 s. After 45 days of operation a stable nitrogen removal rate of 0.67 ± 0.06 kg N m(-3) biofilter d(-1), an ammonia removal efficiency of 99%, a removal of 75-80% of the total nitrogen, and negligible NO/N(2)O productions were obtained at water flow rates of 1.3 ± 0.4 m(3) m(-2) biofilter section d(-1). Profile measurements revealed that 91% of the total nitrogen activity was taking place in the top 36% of the filter. This study demonstrated for the first time highly effective and sustainable autotrophic ammonia removal in a gas biofilter and therefore shows the appealing potential of the OLAND process to treat ammonia containing gaseous streams.

Response of mixed community anammox biomass against sulfide, nitrite and recalcitrant carbon in terms of inhibition coefficients and functional gene expressions.

Anaerobic ammonium oxidation (anammox) has evolved as a carbon and energy-efficient nitrogen management bioprocess. However, factors such as inhibitory chemicals still challenge the easy operation of this powerful bioprocess. This research systematically evaluated the inhibition kinetics of sulfide, nitrite, and recalcitrant carbon under a genomic framework. The inhibition at the substrate and genetic levels of sulfide, nitrite and recalcitrant carbon on anammox activity was studied using batch tests. Nitrite inhibition of anammox followed substrate inhibition and was best described by the Aiba model with an inhibition coefficient [Formula: see text] of 324.04 mg N/L. Hydrazine synthase (hzsB) gene (anammox biomarker) expression was increased over time when incubated with nitrite up to 400 mg N/L. However, despite having the highest specific nitrite removal (SNR), the expression of hzsB at 100 and 200 mg N/L of nitrite was more muted than in most other samples with lower SNRs. Sulfide severely inhibited anammox activities. The inhibition was fitted with a Monod-based model with a [Formula: see text] of 4.39 mg S/L. At a sulfide concentration of 5 mg/L, the hzsB expression decreased throughout the experiment from its original value at he beginning. Recalcitrant carbon of filtrate from thermal hydrolysis process pretreated anaerobic digester had a minimal effect on maximum specific anammox activity (MSAA), and thus the value of the inhibition coefficient could not be calculated. At the same time, its hzsB expression profile was similar to that in the control. Resiliency and recovery tests indicated that the inhibition of nitrite (up to 400 mg N/L) and recalcitrant carbon (in 100% filtrate) were reversible. About 32% of MSAA was recovered after repeated exposures to sulfide at 2.5 mg/L, while at 5 mg/L, the inhibition was irreversible. Findings from this study will be helpful for the successful design and implementation of anammox in full-scale applications.

Partial denitrification-anammox (PdNA) application in mainstream IFAS configuration using raw fermentate as carbon source.

This research examined the feasibility of raw fermentate for mainstream partial denitrification-anammox (PdNA) in a pre-anoxic integrated fixed-film activated sludge (IFAS) process. Fermentate quality sampled from a full-scale facility was highly dynamic, with 360-940 mg VFA-COD/L and VFA/soluble COD ratios ranging from 24% to 48%. This study showed that PdNA selection could be achieved even when using low quality fermentate. Nitrate residual was identified as the main factor driving the PdN efficiency, while management of nitrate conversion rates was required to maximize overall PdNA rates. AnAOB limitation was never observed in the IFAS system. Overall, this study showed PdN efficiencies up to 38% and PdNA rates up to 1.2 ± 0.7 g TIN/m2 /d with further potential for improvements. As a result of both PdNA and full denitrification, this concept showed the potential to save 48-89% methanol and decrease the carbon footprint of water resource recovery facilities (WRRF) by 9-15%. PRACTITIONER POINTS: Application of PdNA with variable quality fermentate is feasible when the nitrate residual concentration is increased to enhance PdN selection. To maximize nitrogen removed through PdNA, nitrate conversion rates need enhancement through optimization of upstream aeration and PdN control setpoints. The IFAS PdNA process was never anammox limited; success depended on the degree of PdN achieved to make nitrite available. Application of PdNA with fermentate can yield 48-89% savings in methanol or other carbon compared with conventional nitrification and denitrification. Integrating PdNA upstream from polishing aeration and anoxic zones guarantees that stringent limits can be met (<5 mg N/L).