Sediment DNA metabarcoding and morphology provide complementary insight into macrofauna and meiobenthos response to environmental gradients in an Arctic glacial fjord.

Arctic fjords ecosystems are highly dynamic, with organisms exposed to various natural stressors along with productivity clines driven by advection of water masses from shelves. The benthic response to these environmental clines has been extensively studied using traditional, morphology-based approaches mostly focusing on macroinvertebrates. In this study we analyse the effects of glacially mediated disturbance on the biodiversity of benthic macrofauna and meiobenthos (meiofauna and Foraminifera) in a Svalbard fjord by comparing morphology and eDNA metabarcoding. Three genetic markers targeting metazoans (COI), meiofauna (18S V1V2) and Foraminifera (18S 37f) were analyzed. Univariate measures of alpha diversity and multivariate compositional dissimilarities were calculated and tested for similarities in response to environmental gradients using correlation analysis. Our study showed different taxonomic composition of morphological and molecular datasets for both macrofauna and meiobenthos. Some taxonomic groups while abundant in metabarcoding data were almost absent in morphology-based inventory and vice versa. In general, species richness and diversity measures in macrofauna morphological data were higher than in metabarcoding, and similar for the meiofauna. Both methodological approaches showed different patterns of response to the glacially mediated disturbance for the macrofauna and the meiobenthos. Macrofauna showed an evident distinction in taxonomic composition and a dramatic cline in alpha diversity indices between the outer and inner parts of fjord, while the meiobenthos showed a gradual change and more subtle responses to environmental changes along the fjord axis. The two methods can be seen as complementing rather than replacing each other. Morphological approach provides more accurate inventory of larger size species and more reliable quantitative data, while metabarcoding allows identification of inconspicuous taxa that are overlooked in morphology-based studies. As different taxa may show different sensitivities to environmental changes, both methods shall be used to monitor marine biodiversity in Arctic ecosystems and its response to dramatically changing environmental conditions.

Assessment of exposure of benthic organisms to selected organochlorine pollutants in the west Spitsbergen fjords.

Climate-related changes in environmental conditions, such as reduction of sea ice, intensive glacier retreat, and increasing summer precipitation, directly influence the arctic marine environment and, therefore, the organisms living there. Benthic organisms, being an important food source for organisms from higher trophic levels, constitute an important part of the Arctic trophic network. Moreover, the long lifespan and limited mobility of some benthic species make them suitable for the study of the spatial and temporal variability of contaminants. In this study, organochlorine pollutants (polychlorinated biphenyls (PCBs) and hexachlorobenzene (HCB)) were measured in benthic organisms collected in three fjords of western Spitsbergen. Two of these were recommended by the Marine Biodiversity and Ecosystem Functioning (MARBEF) Network of Excellence as European flagship sites, namely Hornsund as the Biodiversity Inventory and Kongsfjorden as the Long-Term Biodiversity Observatory. Adventfjorden, with notable human activity, was also studied. Æ©7 PCB and HCB concentrations in sediments were up to 2.4 and 0.18 ng/g d.w. respectively. Concentrations of Æ©7 PCBs and HCB measured in collected benthic organisms were up to 9.1 and 13 ng/g w.w., respectively. In several samples (41 of 169) the concentrations of ∑7 PCBs were below the detection limit values, yet nevertheless the results of the research show effective accumulation of target organochlorine contaminants by many Arctic benthic organisms. Important interspecies differences were observed. Free-living, mobile taxa, such as shrimp Eualus gaimardii, have accumulated a large quantity of contaminants, most probably due to their predatory lifestyle. ∑7 PCB and HCB concentrations were both significantly higher in Hornsund than in Kongsfjorden. Biomagnification occurred in 0 to 100 % of the predator-prey pairs, depending on the congener analyzed. Although the sampled organisms were proved to have accumulated organochlorine contaminants, the measured levels can be considered low, and not posing a substantial threat to the biota.

The interplay between plankton and particles in the Isfjorden waters influenced by marine- and land-terminating glaciers.

Climate-induced glacial retreat in the Arctic results in an increased supply of meltwater with suspended terrigenous material into the marine environment. Despite increasing research efforts, effects of glacial retreat on functioning of plankton are not well documented and understood. Thus, we studied a hydro-optical seawater regime along with particle/plankton concentrations and composition structure in a high Arctic fjord (Isfjorden, West Spitsbergen) during mid-summer in 2019. This comprehensive study of the upper 50 m water layer presented a sharp distinction between 'muddy' waters influenced by glacial and river runoff and 'clear' open fjordic waters in the form of a notable difference in chlorophyll a concentrations, extent of euphotic zone depth, turbidity, inorganic/organic particle concentrations, and water colour. In this study, we present that the effects of glacial retreat on Arctic pelagial depend not only on different types of glaciers (marine- and land-terminating), but presumably, also on fjord topography and exposure to oceanic water inflow. The contrasting glacial, hydrological, and topographical conditions had different effects on the share of zooplankton and marine snow. Despite adaptation of the planktonic communities in the Arctic to high sediment loads and resultant light limitations, our study shows that continuing retreat of tidewater glaciers will have negative effect on planktonic communities especially in enclosed shallow fjord branches. Moreover, seawater darkening due to high turbidity could negatively affect tactile predators, such as gelatinous zooplankton. Additional division of plankton into functional groups typically used in the biogeochemical models demonstrated that diatoms, flagellates and mesozooplankton are influenced by suspended matter, whereas microzooplankton are highly adaptive to increased sediment loads. Since we investigated the largest Svalbard fjord system and incorporated multiple components of the pelagic realm, the current study delivers important recommendations for including marine snow and gelatinous zooplankton in ecosystem models applied in polar regions.