[Study on Deproteinization of Polysaccharides from Dioscorea nipponica].

Objective: To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides. Methods: The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated. Results: PapainSevage method was the best one, in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows, hydrolysing the substrates with 2. 0% papain( p H 7. 0) at 55 ℃ for 122 min, and one times with Sevage method. Conclusion: The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.

[Study on the characteristic of assimilating nitrogenous phosphorous fertilizer and the accumulation disciplinarian of total tanshinons of Salvia miltiorrhiza].

OBJECTIVE: To confirm the amount of fertilizer and the ration of fertilizer. METHOD: Through the experiment of planting Salvia miltiorrhiza in pot and in field were carried out. RESULT: When N:P = 1:1, The production was 1.8 times more than the contrast group in plotting experiment, and in field the production of the highest fertilizer plot were 2.5 times more than the contrast and the higher and high fertilizer plot is 2.25 and 1.2 times respective were than those in the contrast group. CONCLUSION: The proper ration of nitrogenous fertilizer and phosphorous fertilizer is 1:1. Nitrogen shows negative effects to the accumulation of tanshinon IIA, the more the nitrogen ous fertilizer, The less the content of tanshinon IIA. In contrast, phosphor ous fertilizer shows good effects on the accumulation of tanshinon IIA. Phosphorous fertilizer could alleviate the decline of the content of tanshinon IIA by using nitrogenous fertilizer. The accumulation peak of the tanshinon IIA emerge in the period of 150 d.