Chicks produce consonant, sometimes jazzy, sounds.

Several animal species prefer consonant over dissonant sounds, a building block of musical scales and harmony. Could consonance and dissonance be linked, beyond music, to the emotional valence of vocalizations? We extracted the fundamental frequency from calls of young chickens with either positive or negative emotional valence, i.e. contact, brood and food calls. For each call, we calculated the frequency ratio between the maximum and the minimum values of the fundamental frequency, and we investigated which frequency ratios occurred with higher probability. We found that, for all call types, the most frequent ratios matched perfect consonance, like an arpeggio in pop music. These music-like intervals, based on the auditory frequency resolution of chicks, cannot be miscategorized into contiguous dissonant intervals. When we analysed frequency ratio distributions at a finer-grained level, we found some dissonant ratios in the contact calls produced during distress only, thus sounding a bit jazzy. Complementing the empirical data, our computational simulations suggest that physiological constraints can only partly explain both consonances and dissonances in chicks' phonation. Our data add to the mounting evidence that the building blocks of human musical traits can be found in several species, even phylogenetically distant from us.

Unlocking the symmetric transfer of irrelevant information: gene-environment interplay and enhanced interhemispheric cross-talk.

Hemispheric specialization influences stimulus processing and behavioural control, affecting responses to relevant stimuli. However, most sensory input is irrelevant and must be filtered out to prevent interference with task-relevant behaviour, a process known as habituation. Despite habituation's vital role, little is known about hemispheric specialization for this brain function. We conducted an experiment with domestic chicks, an elite animal model to study lateralization. They were exposed to distracting visual stimuli while feeding when using binocular or monocular vision. Switching the viewing eye after habituation, we examined if habituation was confined to the stimulated hemisphere or shared across hemispheres. We found that both hemispheres learned equally to ignore distracting stimuli. However, embryonic light stimulation, influencing hemispheric specialization, revealed an asymmetry in interhemispheric transfer of the irrelevant information discarded via habituation. Unstimulated chicks exhibited a directional bias, with the right hemisphere failing to transfer distracting stimulus information to the left hemisphere, while transfer from left to right was possible. Nevertheless, embryonic light stimulation counteracted this asymmetry, enhancing communication from the right to the left hemisphere and reducing the pre-existing imbalance. This sharing extends beyond hemisphere-specific functions and encompasses a broader representation of irrelevant events.

Attentional capture in emotion comparison is orientation independent.

Recent findings on emotion comparison show a typical pattern of motor reactivity rising from attentional capture. When pairs of emotional faces are presented simultaneously, the most intense emotional face is recognized faster (Emotional Semantic Congruency-ESC effect). Furthermore, a global response speed advantage for emotional pairs with positive rather than negative average emotion intensity is observed (i.e., emotional size effect), with the choice for the happiest face resulting in a faster response than the choice for the angriest face within the pair (i.e., the happiness advantage). In two experiments, we asked whether these effects are orientation dependent, and thus linked to whether face processing is holistic or part-based. Participants were asked to choose the angriest/happiest face in emotional pairs displayed either in upright or inverted orientation and including (Experiment 1) or not including (Experiment 2) a neutral face. Beyond an overall facilitation for upright relative to inverted pairs, results showed orientation independent ESC and emotional size effects. Furthermore, the happiness advantage was present in emotional pairs of Experiment 2 but not in emotional pairs of Experiment 1, independently from face orientation. Together, results suggest that attentional capture in emotion comparison is immaterial on the type of face processing, being orientation invariant.

Long-lasting generalization triggered by a single trial event in the invasive crayfish Procambarus clarkii.

Behavioural flexibility allows adaptation to environmental changes, a situation that invasive species have often to face when colonizing new territories. Such flexibility arises from a set of cognitive mechanisms among which generalization plays a key role, as it allows the transfer of past solutions to solve similar new problems. By means of a habituation paradigm, we studied generalization in the invasive crayfish Procambarus clarkii Once crayfish had habituated their defensive response to a specific water jet, we tested whether habituation transferred to a new type of water jet. Although habituation did not generalize when the new stimulus was initially presented, it surprisingly emerged 15 and 45 days later. Hence, remarkably, in Pclarkii, a single presentation of a new event was sufficient to trigger a long-lasting form of learning generalization from previous similar stimuli, a cognitive ability that may concur in providing adaptive advantages to this invasive species.

Context learning before birth: evidence from the chick embryo.

Learning contextual information to form associative memories with stimuli of interest is an important brain function in both human and non-human animals. Intuitively, one would expect that such a sophisticated cognitive skill develops postnatally, as the organism starts exploring the surrounding environment to search for significant contingencies among stimuli. Here we show, instead, that even before hatching, domestic chicks are capable of forming associative memories between discrete alerting sounds and the surrounding context, as attested by the fact that habituation of the freezing response to the sounds is affected by the context of stimulation. This finding indicates that, while in the egg, chicks recognize and learn the context in which they are stimulated. Hence, context learning in chicks is an innate brain function already active before birth, which can provide an immediate survival advantage to the newborns of this precocial avian species.

Rapid plasticity attenuation soon after birth revealed by habituation in newborn chicks.

Habituation reflects a form of experience-dependent plasticity whereby the organism progressively learns to ignore the irrelevant information repeatedly encountered. Here, we measured the freezing response to a repeated loud noise in three groups of newborn chicks (Gallus gallus) of different ages (1-2 Day old, 2-3 Day old, and 3-4 Day old) to investigate the ontogeny of habituation in this avian species. Habituation was already present 1 Day after hatching, revealing that the neural mechanisms underlying this form of plasticity are immediately active. Unexpectedly, however, we also found that in the second Day of stimulation the amount of learning was significantly attenuated in chicks of 3-4 days of age as compared to the younger animals, thus showing that 24-48 hr of maturation were sufficient to reduce plasticity. While previous findings in other animal species have proved the existence of a precocious critical period of plasticity in early cortical areas by means of sensory deprivation, our results demonstrate that during the initial development of an intact avian brain also the degree of plasticity underlying a learning process like habituation is maximal soon after birth, and then is subject to a rapid attenuation.

Goal-directed behavior in Tenebrio molitor larvae.

Can signs of intentional behavior be traced in an insect larva, traditionally thought to be driven only by mere reflexes? We trained Tenebrio molitor coleoptera larvae in a uniform Y-maze to prefer one target branch to get access to food, observing their ability to learn and retain access to the reward-associated side for up to 24 h. During reward devaluation, the reward food (experimental group) and a different food (control group) were paired with an aversive stimulus in a new environment. When tested again in the Y-maze, mealworms of the experimental group significantly reduced their visits to the target branch, whereas mealworms of the control group did not. Importantly, we found that the larvae did not have to experience the unpleasant consequences directly in the target branch to halt their behavior, as the exposure to the aversive taste occurred in a separate unfamiliar context. This is evidence that the mealworms formed a mental representation of action-consequence relationships, demonstrating flexible control of their actions to achieve desired outcomes at an early stage of their development.

The temporal dynamics of emotion comparison depends on low-level attentional factors.

Humans are predisposed to attend to emotions conveyed by facial expressions. However, compulsory attraction to emotions gets challenging when multiple emotional stimuli compete for attention, as in the emotion comparison task. In this task, participants are asked to choose which of two simultaneously presented faces displays the most positive (happiest) or negative (angriest) emotion. Participants usually respond faster to the face displaying the most intense emotion. This effect is stronger for face pairs that contain globally positive rather than negative emotional faces. Both effects are consistent with an attentional capture phenomenon driven by the perceptual salience of facial expressions. In the present experiment, we studied the temporal dynamics of attentional capture in the emotion comparison task by tracking participants' eye movements using gaze-contingent displays and responses. Our results show that, on the first fixation, participants were more accurate and dwelled longer on the left target face when it displayed the most intense emotion within the pair. On the second fixation, the pattern was reversed, with higher accuracy and longer gaze time on the right target face. Overall, our pattern of gazing behavior indicates that the typical results observed in the emotion comparison task arise from the optimal combination over time of two low-level attentional factors: the perceptual salience of emotional stimuli and the scanning habit of participants.

Octopus vulgaris Exhibits Interindividual Differences in Behavioural and Problem-Solving Performance.

By presenting individual Octopus vulgaris with an extractive foraging problem with a puzzle box, we examined the possible correlation between behavioural performances (e.g., ease of adaptation to captive conditions, prevalence of neophobic and neophilic behaviours, and propensity to learn individually or by observing conspecifics), biotic (body and brain size, age, sex) and abiotic (seasonality and place of origin) factors. We found more neophilic animals showing shorter latencies to approach the puzzle box and higher probability of solving the task; also, shorter times to solve the task were correlated with better performance on the individual learning task. However, the most neophilic octopuses that approached the puzzle box more quickly did not reach the solution earlier than other individuals, suggesting that strong neophilic tendency may lead to suboptimal performance at some stages of the problem-solving process. In addition, seasonal and environmental characteristics of location of origin appear to influence the rate of expression of individual traits central to problem solving. Overall, our analysis provides new insights into the traits associated with problem solving in invertebrates and highlights the presence of adaptive mechanisms that promote population-level changes in octopuses' behavioural traits.

Gastrointestinal Disorders and Food Selectivity: Relationship with Sleep and Challenging Behavior in Children with Autism Spectrum Disorder.

The aim of this study was to evaluate the interaction between gastrointestinal (GI) disorders, sleep problems, and challenging behaviors in children with a diagnosis of Autism Spectrum Disorder (ASD) and their effect on parental stress. The secondary objective was to assess the frequency and type of GI and feeding disorders in a sample of children with ASD through a multidisciplinary assessment and, finally, to investigate families' perceptions and satisfaction with the proposed multidisciplinary approach. All children underwent a comprehensive gastroenterological and neuropsychiatric evaluation supported by standardized questionnaires. Pediatric gastroenterologists, specifically trained in Applied Behavior Analysis (ABA), provided advice for parent-delivered behavioral intervention for food selectivity. Thirty-six children with an autism diagnosis (29 males, age 4.5 +/-2.2 years, mean +/- SD) were enrolled. A positive correlation between sleep problems and aggressive behavior was found, and this association was stronger in children experiencing more problematic mealtime behaviors (b = 0.788, p = 0.014). Sleep difficulties were associated with stereotyped behaviors and parent-perceived stress. Parents interviewed about the gastroenterology visit perceived this multidisciplinary approach as helpful in addressing food selectivity. This study shows that sleep and mealtime issues can have a synergistic negative impact on ASD symptoms. A multidisciplinary approach and an integrated assessment of GI, feeding problems, and sleep disorders could be helpful in diagnosing comorbidities and to provide targeted advice to parents.

Short-Term Outcomes of an ESDM Intervention in Italian Children with Autism Spectrum Disorder following the COVID-19 Lockdown.

The COVID-19 pandemic caused a temporary lockdown period in Italy, during which the delivery of in-person treatment for children with autism spectrum disorder (ASD) in public health services was discontinued. This occurrence represented a crucial challenge for both families and professionals. We assessed the short-term outcomes of a sample of 18 children who received an early intervention with the Early Start Denver Model (ESDM), delivered at low intensity over one year in the pre-pandemic period, after six months of interruption of in-presence treatment due to lockdown restrictions. Children who received the ESDM treatment maintained their gains in sociocommunicative skills and did not exhibit any developmental regression. Additionally, there was evidence of a decrease in the restrictive and repetitive behavior (RRB) domain. The parents, who were already familiar with the principles of the ESDM, only received telehealth support from therapists that aimed to sustain the gains already achieved. We believe that it is always helpful to support parents in their daily lives by implementing interactional and play skills with their children to integrate and consolidate the results obtained in the individual interventions conducted by experienced therapists.

Individual differences in habituation: Innate covariation between habituation, exploration, and body size in naïve chicks (Gallus gallus).

Habituation to novel stimuli has been associated with behavioural differences among individuals in numerous animal species. Because the habituation mechanisms depend on previous experiences with a stimulus, one would expect individuals to develop their habituation capacity based on the life experiences that also shape their behavioural traits. And indeed, in adult lizards, exploratory behaviour and body size correlates with habituation. However, here we show that the same factors correlate with habituation of domestic chicks reared under controlled laboratory conditions and tested in the first 3 days after hatching. This result indicates that the covariation between habituation, exploration, and body size does not necessarily depend on experience. Rather, it represents an innate association between exploratory behaviour and risk assessment, which may provide an immediate survival advantage to new-borns of this precocial avian species.

Context-Specific Habituation: A Review.

Habituation consists of the progressive response decrement to a repeated stimulation, a response decline that is not accounted for by sensory or motor fatigue. Together with sensitization, habituation has been traditionally considered to be a prototypical example of non-associative learning, being affected only by the features of the stimulation, as for instance its intensity or frequency. However, despite this widespread belief, evidence exists showing that habituation can be specific to the context of the stimulation, thus suggesting that habituation can have an associative nature. Such an unexpected characteristic of habituation was in fact predicted by a theoretical model of associative learning proposed by Wagner in a series of works that appeared in the late 1970s. Here, we critically review the experimental data that since then have been accumulated in support of this hypothesis. What emerges from the literature is that context-specific habituation is common to several animal species and that the ability to form an association between the habituating stimulus and its context is independent of the complexity of the animal's nervous system. Finally, context-specific habituation is observed for a variety of organism's responses, ranging from visceral to motor and mental activities.

Feasibility and Outcomes of the Early Start Denver Model Delivered within the Public Health System of the Friuli Venezia Giulia Italian Region.

The Early Start Denver Model (ESDM) is an evidence-based early intervention model for young children with autism spectrum disorder (ASD). It is crucial to investigate the feasibility of the ESDM in community settings in contexts that are culturally different from American universities in which the model was originally developed. The aim was to further evaluate the effectiveness of the ESDM delivered within the Italian community setting at low intensity. We compared a group aged 19 to 43 months receiving the ESDM for 2 h per week over the course of 1 year with a concurrent, comparable, non-randomized control group receiving treatment as usual (TAU). Children were evaluated at baseline (T0) and after 6 months (T1) and 12 months (T2) of intervention. Feasibility was evaluated by parent and therapist questionnaires, retention rate, and therapist treatment fidelity. Both groups made similar gains in cognition and language abilities. The ESDM group made larger improvement in domains measured by the ESDM Curriculum Checklist, including communication, social skills, and maladaptive behaviors. Feasibility seemed well supported by retentions, therapists and parent satisfaction, and treatment fidelity. Our study further supports the feasibility of the ESDM implemented within the Italian public health system and suggests a better response in the ESDM-treated group than in the control group.

Short-term memory in habituation and dishabituation of newborn chicks' freezing response.

Cognitive models of habituation and dishabituation postulate that the latter is attributable to the perturbation of the model of the repeated stimulation stored in short-term memory (STM) by the occurrence of a new stimulus, called dishabituator. However, although both behavioral phenomena depend on STM, previous studies in Aplysia have found that dishabituation seems to require further steps of development of the STM system to emerge. Here, we addressed whether this is a universal condition for the appearance of the 2 forms of learning, namely whether dishabituation must necessarily follow habituation. To this aim, we studied habituation and dishabituation of the freezing response to a sudden acoustic stimulation in newly hatched chicks (1 day old vs. 3 days old). The results showed that in chicks, dishabituation was fully present a few hours after hatching, a pattern of results indicating that, in this precocial avian species, habituation and dishabituation share the same developmental trajectory and the underlying STM mechanisms are simultaneously operative soon after birth. (PsycINFO Database Record (c) 2018 APA, all rights reserved).