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OBJECTIVE: This study aimed to estimate whether the potential short-term advantages of laparoscopic pancreaticoduodenectomy (LPD) could allow patients to recover in a more timely manner and achieve better long-term survival than with open pancreaticoduodenectomy (OPD) in patients with pancreatic or periampullary tumors. BACKGROUND: LPD has been demonstrated to be feasible and may have several potential advantages over OPD in terms of shorter hospital stay and accelerated recovery than OPD. METHODS: This noninferiority, open-label, randomized clinical trial was conducted in 14 centers in China. The initial trial included 656 eligible patients with pancreatic or periampullary tumors enrolled from May 18, 2018, to December 19, 2019. The participants were randomized preoperatively in a 1:1 ratio to undergo either LPD (n=328) or OPD (n=328). The 3-year overall survival (OS), quality of life, which was assessed using the 3-level version of the European Quality of Life-5 Dimensions, depression, and other outcomes were evaluated. RESULTS: Data from 656 patients [328 men (69.9%); mean (SD) age: 56.2 (10.7) years] who underwent pancreaticoduodenectomy were analyzed. For malignancies, the 3-year OS rates were 59.1% and 54.3% in the LPD and OPD groups, respectively ( P =0.33, hazard ratio: 1.16, 95% CI: 0.86-1.56). The 3-year OS rates for others were 81.3% and 85.6% in the LPD and OPD groups, respectively ( P =0.40, hazard ratio: 0.70, 95% CI: 0.30-1.63). No significant differences were observed in quality of life, depression and other outcomes between the 2 groups. CONCLUSION: In patients with pancreatic or periampullary tumors, LPD performed by experienced surgeons resulted in a similar 3-year OS compared with OPD. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT03138213.
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Laparoscopía , Neoplasias Pancreáticas , Masculino , Humanos , Persona de Mediana Edad , Pancreaticoduodenectomía/métodos , Estudios de Seguimiento , Calidad de Vida , Laparoscopía/métodos , Tiempo de Internación , Estudios Retrospectivos , Complicaciones Posoperatorias/cirugíaRESUMEN
Five countries in the Lancang-Mekong region, including Myanmar, Laos, Thailand, Cambodia, and Vietnam, are facing the threat of deforestation, despite having a high level of forest coverage. Quantitatively assessing the forest ecosystem status and its variations based on remote sensing products for vegetation parameters is a crucial prerequisite for the ongoing phase of our future project. In this study, we analyzed forest health in the year 2020 using four vegetation indicators: forest coverage index (FCI), leaf area index (LAI), fraction of green vegetation cover (FVC), and gross primary productivity (GPP). Additionally, we introduced an ecosystem quality index (EQI) to assess the quality of forest health. To understand the long-term trends in the vegetation indicators and EQI, we also performed a linear regression analysis from 2010 to 2020. The results revealed that Laos ranked as the top-performing country for forest ecosystem status in the Lancang-Mekong region in 2020. However, the long-term trend analysis results showed that Cambodia experienced the most significant decline across all indicators, while Vietnam and Thailand demonstrated varying degrees of improvement. This study provides a quality assessment of forest health and its variations in the Lancang-Mekong region, which is crucial for implementing effective conservation strategies.
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Ecosistema , Tecnología de Sensores Remotos , Bosques , Cambodia , TailandiaRESUMEN
Chromatin accessibility is essential for transcriptional activation of genomic regions. It is well established that transcription factors (TFs) and histone modifications (HMs) play critical roles in chromatin accessibility regulation. However, there is a lack of studies that quantify these relationships. Here we constructed a two-layer model to predict chromatin accessibility by integrating DNA sequence, TF binding, and HM signals. By applying the model to two human cell lines (GM12878 and HepG2), we found that DNA sequences had limited power for accessibility prediction, while both TF binding and HM signals predicted chromatin accessibility with high accuracy. According to the HM model, HM features determined chromatin accessibility in a cell line shared manner, with the prediction power attributing to five core HM types. Results from the TF model indicated that chromatin accessibility was determined by a subset of informative TFs including both cell line-specific and generic TFs. The combined model of both TF and HM signals did not further improve the prediction accuracy, indicating that they provide redundant information in terms of chromatin accessibility prediction. The TFs and HM models can also distinguish the chromatin accessibility of proximal versus distal transcription start sites with high accuracy.
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Cromatina , Epigenómica , Sitios de Unión , Cromatina/genética , Inmunoprecipitación de Cromatina , Simulación por Computador , Humanos , Unión ProteicaRESUMEN
BACKGROUND: Pancreatic cancer (PC) has become the fourth most lethal among human cancers. Long noncoding RNAs (lncRNAs) have been reported to play a role in the progression of a variety of cancers. However, the role of lncRNA SNHG1 in PC is not clear. METHODS: Real-time Quantitative PCR Detection System (qPCR) was used to detect the expression of SNHG1 in PC cells. Then, the SNHG1 knockdown cell was constructed with si-SNHG1. AsPC-1 and PANC1 cells were used to analyze the ability of cell proliferation, invasion, and migration. MTT assay was used to analyze the proliferation ability. Transwell experiments and wound healing experiments were used to detect the capacity of invasion and migration. Finally, Western blot analysis was used to explore the mechanism of SNHG1 in PC. RESULTS: SNHG1 was significantly upregulated in PC cells. Knockdown of SNHG1 could obviously suppress cell proliferation, invasion, and migration. Furthermore, SNHG1 knockdown inhibited the activation of the Notch-1 signaling pathway and inhibited the expression of N-cadherin, Hes1, Vimentin, Notch-1. The inhabitation was reversed when Notch-1 was overexpressed in si-SNHG1 cells. CONCLUSION: The lncRNA SNHG1 promotes cell growth and metastasis in PC through activation of the Notch-1 signaling pathway in PC.
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Neoplasias Pancreáticas/metabolismo , Neoplasias Pancreáticas/patología , ARN Largo no Codificante/metabolismo , Receptor Notch1/fisiología , Animales , Apoptosis/genética , Apoptosis/fisiología , Movimiento Celular/genética , Movimiento Celular/fisiología , Proliferación Celular/genética , Proliferación Celular/fisiología , Regulación Neoplásica de la Expresión Génica/genética , Regulación Neoplásica de la Expresión Génica/fisiología , Células HT29 , Humanos , Etiquetado Corte-Fin in Situ , Ratones , Ratones Desnudos , Neoplasias Pancreáticas/genética , ARN Largo no Codificante/genética , Receptor Notch1/genética , Receptor Notch1/metabolismo , Transducción de Señal/genética , Transducción de Señal/fisiologíaRESUMEN
BACKGROUND/AIMS: Due to the semi-permeable membrane and biocompatibility, microcapsules have a very promising future in cell transplantation, drug carrier and large-scale cell culture. However, the current design prevents it from playing a role in tissue scaffold. METHODOLOGY: To address this pitfall, we invented a self-assembled botryoidal microcapsule by means of electrostatic interactions. The diameter of the scaffold was adjustable between 1 and 5cm, while that of the standard microcapsule is ordinarily 500µm. RESULTS: Tests showed that it had better mechanical strength, as well as characteristics of biocompatibility and permeability. CONCLUSIONS: A botryoidal microcapsule is also effective at blocking the entrance of IgG and has the potential to function both as a favorable carrier and as a scaffold for artificial organs.
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Alginatos/química , Materiales Biocompatibles , Quitosano/química , Ingeniería de Tejidos/métodos , Andamios del Tejido , Albúminas/metabolismo , Animales , Biomarcadores/metabolismo , Cápsulas , Células Cultivadas , Quitosano/análogos & derivados , Ácido Glucurónico/química , Hepatocitos/metabolismo , Ácidos Hexurónicos/química , Inmunoglobulina G/metabolismo , L-Lactato Deshidrogenasa/metabolismo , Masculino , Ratones , Ratones Endogámicos BALB C , Permeabilidad , Electricidad Estática , Factores de TiempoRESUMEN
We presented a framework to evaluate the land use transformations over the Eurasian Steppe (EUS) driven by human activities from 2000 to 2020. Framework involves three main components: (1) evaluate the spatial-temporal dynamics of land use transitions by utilizing the land change modeler (LCM) and remote sensing data; (2) quantifying the individual contributions of climate change and human activities using improved residual trend analysis (IRTA) and pixel-based partial correlation coefficient (PCC); and (3) quantifying the contributions of land use transitions to Leaf Area Index Intensity (LAII) by using the linear regression. Research findings indicate an increase in cropland (+1.17 % = 104,217 km2) over EUS, while a - 0.80 % reduction over Uzbekistan and - 0.16 % over Tajikistan. From 2000 to 2020 a slight increase in grassland was observed over the EUS region by 0.05 %. The detailed findings confirm an increase (0.24 % = 21,248.62 km2) of grassland over the 1st half (2000-2010) and a decrease (-0.19 % = -16,490.50 km2) in the 2nd period (2011-2020), with a notable decline over Kazakhstan (-0.54 % = 13,690 km2), Tajikistan (-0.18 % = 1483 km2), and Volgograd (-0.79 % = 4346 km2). Area of surface water bodies has declined with an alarming rate over Kazakhstan (-0.40 % = 10,261 km2) and Uzbekistan (-2.22 % = 8943 km2). Additionally, dominant contributions of human activities to induced LULC transitions were observed over the Chinese region, Mongolia, Uzbekistan, and Volgograd regions, with approximately 87 %, 83 %, 92 %, and 47 %, respectively, causing effective transitions to 12,997 km2 of cropland, 24,645 km2 of grassland, 16,763 km2 of sparse vegetation in China, and 12,731.2 km2 to grassland and 15,356.1 km2 to sparse vegetation in Mongolia. Kazakhstan had mixed climate-human impact with human-driven transitions of 48,568 km2 of bare land to sparse vegetation, 27,741 km2 to grassland, and 49,789 km2 to cropland on the eastern sides. Southern regions near Uzbekistan had climatic dominancy, and 8472 km2 of water bodies turned into bare soil. LAII shows an increasing trend rate of 0.63 year-1, particularly over human-dominant regions. This study can guide knowledge of oscillations and reduce adverse impacts on ecosystems and their supply services.
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Ecosistema , Monitoreo del Ambiente , Humanos , Tecnología de Sensores Remotos , Actividades Humanas , Agua , ChinaRESUMEN
As the most influential atmospheric oscillation on Earth, the El Niño/Southern Oscillation (ENSO) can significantly change the surface climate of the tropics and subtropics and affect the high latitudes of northern hemisphere areas through atmospheric teleconnection. The North Atlantic Oscillation (NAO) is the dominant pattern of low-frequency variability in the Northern Hemisphere. As the dominant oscillations in the Northern Hemisphere, the ENSO and NAO have been affecting the giant grassland belt in the world, the Eurasian Steppe (EAS), in recent decades. In this study, the spatio-temporal anomaly patterns of grassland growth in the EAS and their correlations with the ENSO and NAO were investigated using four long-term leaf area index (LAI) and one normalized difference vegetation index (NDVI) remote sensing products from 1982 to 2018. The driving forces of meteorological factors under the ENSO and NAO were analyzed. The results showed that grassland in the EAS has been turning green over the past 36 years. Warm ENSO events or positive NAO events accompanied by increased temperature and slightly more precipitation promoted grassland growth, and cold ENSO events or negative NAO events with cooling effects over the whole EAS and uneven precipitation decreased deteriorated the EAS grassland. During the combination of warm ENSO and positive NAO events, a more severe warming effect caused more significant grassland greening. Moreover, the co-occurrence of positive NAO with cold ENSO or warm ENSO with negative NAO kept the characteristic of the decreased temperature and rainfall in cold ENSO or negative NAO events, and deteriorate the grassland more severely.
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Cambio Climático , El Niño Oscilación del Sur , FríoRESUMEN
BACKGROUND: The bioartificial liver (BAL) is considered a possible alternative method for treating liver failure. The core of the BAL system is culturing liver cells in vitro with high density and activity. Microcarrier culture is a mode of high-density culture. We set out to prepare a novel porous microcarrier to improve the activity of liver cells in vitro. METHODS: Chitosan was used to prepare a novel porous spherical microcarrier with interconnected structure. The chitosan porous microcarriers (CPMs) were modified with gelatin to improve their biocompatibility. CPMs were co-cultured with liver cells, HL-7702 (L-02), to evaluate their effect on cell culture. RESULTS: The average size of the CPMs was about 400 µm in diameter and their apertures were less than 30 µm. The pores of the microcarrier were interconnected. After fixation by sodium tripolyphosphate, the structure of the first freeze-dried CPMs was stable. To further improve the biocompatibility, the surface of CPMs was modified with gelatin through chemical crosslinking (GM-CPMs). Comparing the proliferation curves of L-02 cells cultured on simple CPMs, GM-CPMs and tissue culture polystyrene (TCPS, a mode of planar cell culture), the proliferation rates were similar in the first 5 days and the cells proliferated until day 8 in culture with microcarriers. The OD value of liver cells cultured on GM-CPMs was 1.97-fold higher than that on TCPS culture at day 8. Levels of urea and albumin in supernatants of cells cultured on GM-CPMs increased steadily for 8 days, and were clearly higher than those of cells cultured on TCPS (P<0.05). CONCLUSIONS: The novel CPMs were promising microcarriers for hepatocyte culture and the GM-CPM seemed better. Porous microcarrier culture was beneficial for hepatocyte function and activity.
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Técnicas de Cultivo de Célula , Quitosano/química , Hepatocitos/fisiología , Hígado Artificial , Ingeniería de Tejidos/métodos , Albúminas/metabolismo , Línea Celular , Proliferación Celular , Forma de la Célula , Gelatina/química , Hepatocitos/metabolismo , Humanos , Tamaño de la Partícula , Porosidad , Factores de Tiempo , Urea/metabolismoRESUMEN
Melanoma is one of the most aggressive cancer types whose prognosis is determined by both the tumor cell-intrinsic and -extrinsic features as well as their interactions. In this study, we performed systematic and unbiased analysis using The Cancer Genome Atlas (TCGA) melanoma RNA-seq data and identified two gene signatures that captured the intrinsic and extrinsic features, respectively. Specifically, we selected genes that best reflected the expression signals from tumor cells and immune infiltrate cells. Then, we applied an AutoEncoder-based method to decompose the expression of these genes into a small number of representative nodes. Many of these nodes were found to be significantly associated with patient prognosis. From them, we selected two most prognostic nodes and defined a tumor-intrinsic (TI) signature and a tumor-extrinsic (TE) signature. Pathway analysis confirmed that the TE signature recapitulated cytotoxic immune cell related pathways while the TI signature reflected MYC pathway activity. We leveraged these two signatures to investigate six independent melanoma microarray datasets and found that they were able to predict the prognosis of patients under standard care. Furthermore, we showed that the TE signature was also positively associated with patients' response to immunotherapies, including tumor vaccine therapy and checkpoint blockade immunotherapy. This study developed a novel computational framework to capture the tumor-intrinsic and -extrinsic features and identified robust prognostic and predictive biomarkers in melanoma.
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BACKGROUND: The benefit and safety of laparoscopic pancreatoduodenectomy (LPD) for the treatment of pancreatic or periampullary tumours remain controversial. Studies have shown that the learning curve plays an important role in LPD, yet there are no randomised studies on LPD after the surgeons have surmounted the learning curve. The aim of this trial was to compare the outcomes of open pancreatoduodenectomy (OPD) with those of LPD, when performed by experienced surgeons. METHODS: In this multicentre, open-label, randomised controlled trial done in 14 Chinese medical centres, we recruited patients aged 18-75 years with a benign, premalignant, or malignant indication for pancreatoduodenectomy. Eligible patients were randomly assigned (1:1) to undergo either LPD or OPD. Randomisation was centralised via a computer-generated system that used a block size of four. The patients and surgeons were unmasked to study group, whereas the data collectors, outcome assessors, and data analysts were masked. LPD and OPD were performed by experienced surgeons who had already done at least 104 LPD operations. The primary outcome was the postoperative length of stay. The criteria for discharge were based on functional recovery, and analyses were done on a modified intention-to-treat basis (ie, including patients who had a pancreatoduodenectomy regardless of whether the operation was the one they were assigned to). This trial is registered with Clinicaltrials.gov, number NCT03138213. FINDINGS: Between May 18, 2018, and Dec 19, 2019, we assessed 762 patients for eligibility, of whom 656 were randomly assigned to either the LPD group (n=328) or the OPD group (n=328). 31 patients in each group were excluded and 80 patients crossed over (33 from LPD to OPD, 47 from OPD to LPD). In the modified intention-to-treat analysis (297 patients in the LPD group and 297 patients in the OPD group), the postoperative length of stay was significantly shorter for patients in the LPD group than for patients in the OPD group (median 15·0 days [95% CI 14·0-16·0] vs 16·0 days [15·0-17·0]; p=0·02). 90-day mortality was similar in both groups (five [2%] of 297 patients in the LPD group vs six [2%] of 297 in the OPD group, risk ratio [RR] 0·83 [95% CI 0·26-2·70]; p=0·76). The incidence rate of serious postoperative morbidities (Clavien-Dindo grade of at least 3) was not significantly different in the two groups (85 [29%] of 297 patients in the LPD group vs 69 [23%] of 297 patients in OPD group, RR 1·23 [95% CI 0·94-1·62]; p=0·13). The comprehensive complication index score was not significantly different between the two groups (median score 8·7 [IQR 0·0-26·2] vs 0·0 [0·0-20·9]; p=0·06). INTERPRETATION: In highly experienced hands, LPD is a safe and feasible procedure. It was associated with a shorter length of stay and similar short-term morbidity and mortality rates to OPD. Nonetheless, the clinical benefit of LPD compared with OPD was marginal despite extensive procedural expertise. Future research should focus on identifying the populations that will benefit from LPD. FUNDING: National Natural Science Foundation of China and Tongji Hospital, Huazhong University of Science and Technology, China.
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Ampolla Hepatopancreática/cirugía , Laparoscopía/efectos adversos , Neoplasias Pancreáticas/cirugía , Pancreaticoduodenectomía/efectos adversos , Adulto , Anciano , Ampolla Hepatopancreática/patología , Estudios de Casos y Controles , China/epidemiología , Femenino , Humanos , Laparoscopía/métodos , Laparoscopía/mortalidad , Tiempo de Internación/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Evaluación de Resultado en la Atención de Salud , Neoplasias Pancreáticas/patología , Pancreaticoduodenectomía/métodos , Pancreaticoduodenectomía/mortalidad , Alta del Paciente/tendencias , Complicaciones Posoperatorias/epidemiología , Complicaciones Posoperatorias/mortalidad , Cirujanos/estadística & datos numéricosRESUMEN
In the recent few years, plenty of research has shown that microRNA (miRNA) is likely to be involved in the formation of many human diseases. So effectively predicting potential associations between miRNAs and diseases helps to understand the development and treatment of diseases. In this study, an edge perturbation based method is proposed for predicting potential miRNA-disease association (EPMDA). Different from the previous studies, we design an feature vector to describe each edge of a graph by structural Hamiltonian information. Moreover, the extracted features are used to train a multi-layer perception model to predict the candidate disease-miRNA associations. The experimental results on the HMDD dataset show that EPMDA achieves the AUC value of 0.9818 through 5-fold cross-validation, which improves the AUC values by approximately 3.5 percent compared to the latest method DeepMDA. For the leave-one-disease-out cross-validation, EPMDA achieves the AUC value of 0.9371, which improves the AUC values by approximately 7.4 percent compared to DeepMDA. In the case study, we verify the prediction performance of EPMDA on three human diseases. As a result, there are 42, 46, and 41 of the top 50 predicted miRNAs for these three diseases which are confirmed by the published experimental discoveries, respectively.
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Biología Computacional/métodos , Estudios de Asociación Genética/métodos , Predisposición Genética a la Enfermedad/genética , MicroARNs , Algoritmos , Área Bajo la Curva , Humanos , Aprendizaje Automático , MicroARNs/análisis , MicroARNs/genética , MicroARNs/metabolismoRESUMEN
BACKGROUND: This study investigates the characteristics of a special type of cancer of unknown primary site (CUP, type 2), which is a metastasis of a definite pathological diagnosis without a detectable primary site. PATIENTS AND METHODS: Patients diagnosed between 2004 and 2014 were identified from the Surveillance Epidemiology and End Results (SEER) database. The characteristics of type 2 CUP from different sources were analyzed. For each source of type 2 CUP, tumors of the corresponding Tn N0-X M1 stage were used as controls. RESULTS: A total of 8505 patients with type 2 CUP were included in this analysis. Type 2 CUP shows an increasing trend, while type 1 shows the opposite. Type 2 CUPs have significant differences with stage IV of the same pathological primary lesion. Many characteristics influenced the prognosis of type 2 CUP patients, including marital status, age, race, sex, registration time, lymph node metastasis, surgery, chemotherapy, and radiation. CONCLUSION: Our study suggests that identifying the source of metastasis is the key to the selection of treatment and the determination of the prognosis for CUP.
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Neoplasias Primarias Desconocidas/epidemiología , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neoplasias Primarias Desconocidas/diagnóstico , Neoplasias Primarias Desconocidas/patología , Programa de VERFRESUMEN
The present study investigated the expression and potential influence of SHC SH2 domainbinding protein 1 (SHCBP1) in gastric cancer (GC) cells. SHCBP1 is closely related to cell proliferation and cell cycle progression, but its role in GC remains unclear. The TCGA database revealed that SHCBP1 is highly expressed in GC tissues. Furthermore, SHCBP1 was revealed to be highly expressed in GC cell lines MGC803 and SGC7901 cells, and downregulation of SHCBP1 significantly inhibited GC cell proliferation. Furthermore, SHCBP1 expression promoted cell cycle progression and inhibition of apoptosis. Since the CDK4, cyclin D1 and caspase family proteins play important roles in cell cycle and apoptosis regulation, it was examined whether there was an association between SHCBP1 and these signaling pathways in GC. Our results revealed that SHCBP1 promoted cell cycle progression by regulating the CDK4cyclin D1 cascade and suppressed caspase3, caspase PARPdependent apoptotic pathways. Cell invasion and metastasis experiments also revealed that SHCBP1 promoted tumor growth and invasiveness. These tumorpromoting functions of SHCBP1 may provide a potential molecular basis for the diagnosis and targeted therapy of GC.
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Biomarcadores de Tumor/metabolismo , Proteínas Adaptadoras de la Señalización Shc/metabolismo , Neoplasias Gástricas/patología , Apoptosis , Biomarcadores de Tumor/genética , Caspasa 3/metabolismo , Ciclo Celular , Línea Celular Tumoral , Movimiento Celular , Proliferación Celular , Ciclina D1/metabolismo , Quinasa 4 Dependiente de la Ciclina/metabolismo , Regulación hacia Abajo , Regulación Neoplásica de la Expresión Génica , Técnicas de Silenciamiento del Gen , Humanos , ARN Interferente Pequeño/metabolismo , Proteínas Adaptadoras de la Señalización Shc/genética , Transducción de Señal , Estómago/patología , Neoplasias Gástricas/diagnósticoRESUMEN
The existing protein complex detection methods can be broadly divided into two categories: unsupervised and supervised learning methods. Most of the unsupervised learning methods assume that protein complexes are in dense regions of protein-protein interaction (PPI) networks even though many true complexes are not dense subgraphs. Supervised learning methods utilize the informative properties of known complexes; they often extract features from existing complexes and then use the features to train a classification model. The trained model is used to guide the search process for new complexes. However, insufficient extracted features, noise in the PPI data and the incompleteness of complex data make the classification model imprecise. Consequently, the classification model is not sufficient for guiding the detection of complexes. Therefore, we propose a new robust score function that combines the classification model with local structural information. Based on the score function, we provide a search method that works both forwards and backwards. The results from experiments on six benchmark PPI datasets and three protein complex datasets show that our approach can achieve better performance compared with the state-of-the-art supervised, semi-supervised and unsupervised methods for protein complex detection, occasionally significantly outperforming such methods.
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Aprendizaje Automático , Mapeo de Interacción de Proteínas/métodos , Mapas de Interacción de Proteínas , Proteómica/métodos , Programas Informáticos , AlgoritmosRESUMEN
This study was performed to evaluate the feasibility of the splenic bed laparoscopic splenectomy approach (SBLS) for massive splenomegaly (≥30 cm) in patients with hypersplenism secondary to portal hypertension and liver cirrhosis. Patients who underwent laparoscopic splenectomy (LS) from January 2012 to December 2016 were retrospectively reviewed. We performed LS in 83 patients with massive splenomegaly (≥30 cm) secondary to portal hypertension and liver cirrhosis. Of these patients, 37 underwent the SBLS and 46 underwent anterior LS (ALS). Five patients in the ALS group and none in the SBLS group underwent conversion to open surgery. The operation time, intraoperative blood loss volume, transfusion volume, frequency of transfusion, hemorrhage of short gastric vessels, conversion rate, postoperative hospital stay, and incidence of pancreatic fistula were all significantly lower in the SBLS than ALS group (all P < 0.05). No death or postoperative bleeding occurred in the two groups, and there were no significant differences in age, gender, spleen size, hemoglobin level, platelet count, prothrombin time, Child-Pugh class, hypoproteinemia, or ascites (all P > 0.05). The SBLS is more feasible and effective than ALS in patients with massive splenomegaly (≥30 cm) secondary to portal hypertension and liver cirrhosis.
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Hipertensión Portal/complicaciones , Laparoscopía , Cirrosis Hepática/complicaciones , Esplenectomía , Esplenomegalia/cirugía , Adulto , Conversión a Cirugía Abierta , Estudios de Factibilidad , Femenino , Humanos , Tiempo de Internación , Masculino , Persona de Mediana Edad , Tempo Operativo , Estudios Retrospectivos , Esplenomegalia/etiología , Resultado del TratamientoRESUMEN
INTRODUCTION: Laparoscopic splenectomy for massive splenomegaly secondary to liver cirrhosis and portal hypertension in patients with an extremely low platelet count (< 1 × 109/l) presents several challenges. The posterolateral laparoscopic splenectomy approach may be a feasible and safe technique for these patients. AIM: To evaluate the feasibility and safety of the posterolateral laparoscopic splenectomy approach in patients with platelet counts < 1 × 109/l secondary to liver cirrhosis and portal hypertension. MATERIAL AND METHODS: In the period from January 2013 to December 2016, 11 patients with platelet counts < 1 × 109/l secondary to liver cirrhosis and portal hypertension underwent posterolateral laparoscopic splenectomy in our institution. Pre-, peri-, and postoperative medical managements were reviewed retrospectively. RESULTS: Patients' median platelet count was 0.7 × 109/l at the time of inpatient admission. The median operating time was 75 min, and the median intraoperative blood loss was 30 ml. One patient underwent intraoperative transfusion. The median duration of postoperative hospital stay was 5 days. No intra- or postoperative complications ensued, all patients were followed for 12-32 months (median: 24 months), and none had postoperative complications. CONCLUSIONS: The posterolateral laparoscopic splenectomy approach is a feasible, safe technique in the treatment of patients with platelet counts < 1 × 109/l secondary to liver cirrhosis and portal hypertension.
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Essential proteins are the proteins that are indispensable to the survival and development of an organism. Deleting a single essential protein will cause lethality or infertility. Identifying and analysing essential proteins are key to understanding the molecular mechanisms of living cells. There are two types of methods for predicting essential proteins: experimental methods, which require considerable time and resources, and computational methods, which overcome the shortcomings of experimental methods. However, the prediction accuracy of computational methods for essential proteins requires further improvement. In this paper, we propose a new computational strategy named CoTB for identifying essential proteins based on a combination of topological properties, subcellular localization information and orthologous protein information. First, we introduce several topological properties of the protein-protein interaction (PPI) network. Second, we propose new methods for measuring orthologous information and subcellular localization and a new computational strategy that uses a random forest prediction model to obtain a probability score for the proteins being essential. Finally, we conduct experiments on four different Saccharomyces cerevisiae datasets. The experimental results demonstrate that our strategy for identifying essential proteins outperforms traditional computational methods and the most recently developed method, SON. In particular, our strategy improves the prediction accuracy to 89, 78, 79, and 85 percent on the YDIP, YMIPS, YMBD and YHQ datasets at the top 100 level, respectively.
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Biología Computacional/métodos , Proteínas/química , Proteínas/metabolismo , Algoritmos , Mapas de Interacción de ProteínasRESUMEN
Essential proteins are indispensable to the viability and reproduction of an organism. The identification of essential proteins is necessary not only for understanding the molecular mechanisms of cellular life but also for disease diagnosis, medical treatments and drug design. Many computational methods have been proposed for discovering essential proteins, but the precision of the prediction of essential proteins remains to be improved. In this paper, we propose a new method, LBCC, which is based on the combination of local density, betweenness centrality (BC) and in-degree centrality of complex (IDC). First, we introduce the common centrality measures; second, we propose the densities Den1(v) and Den2(v) of a node v to describe its local properties in the network; and finally, the combined strategy of Den1, Den2, BC and IDC is developed to improve the prediction precision. The experimental results demonstrate that LBCC outperforms traditional topological measures for predicting essential proteins, including degree centrality (DC), BC, subgraph centrality (SC), eigenvector centrality (EC), network centrality (NC), and the local average connectivity-based method (LAC). LBCC also improves the prediction precision by approximately 10 percent on the YMIPS and YMBD datasets compared to the most recently developed method, LIDC.
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Mapeo de Interacción de Proteínas/métodos , Proteínas de Saccharomyces cerevisiae/metabolismo , Biología Computacional , Estadística como AsuntoRESUMEN
The expression of Yes-associated protein (YAP) has been reported to be dysregulated in pancreatic cancer. However, its contributions to tumor formation and progression remain to be elucidated. The present study demonstrated that YAP overexpression promoted the epithelialmesenchymal transition (EMT) in a manner associated with pancreatic cancer invasion in vitro. RNA interferencemediated silencing of YAP attenuated cell invasion in vitro. Mechanistically, the present study demonstrated that YAP overexpression fosters pancreatic cancer progression by inducing the EMT in pancreatic cancer cells by activating the AKT cascade, which can counteract the effect of gemcitabine. These data suggested that the YAP acts synergistically to promote pancreatic cancer progression by hyperactivation of AKT signaling. The present study revealed YAP as a potential therapeutic target for pancreatic cancer and a biomarker for predicting gemcitabine treatment response.
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Proteínas Adaptadoras Transductoras de Señales/metabolismo , Resistencia a Antineoplásicos , Transición Epitelial-Mesenquimal , Neoplasias Pancreáticas/patología , Fosfoproteínas/metabolismo , Línea Celular Tumoral , Desoxicitidina/análogos & derivados , Desoxicitidina/farmacología , Progresión de la Enfermedad , Resistencia a Antineoplásicos/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Transición Epitelial-Mesenquimal/efectos de los fármacos , Humanos , Invasividad Neoplásica , Neoplasias Pancreáticas/enzimología , Fenotipo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Transducción de Señal/efectos de los fármacos , Factores de Transcripción , Regulación hacia Arriba/efectos de los fármacos , Proteínas Señalizadoras YAP , GemcitabinaRESUMEN
Chitosan-gelatin B microspheres with an open, interconnected, highly macroporous (100-200 µm) structure were prepared via a three-step protocol combining freeze-drying with an electrostatic and ionic cross-linking method. Saturated tripolyphosphate ethanol solution (85% ethanol) was chosen as the crosslinking agent to prevent destruction of the porous structure and to improve the biostability of the chitosan-gelatin B microspheres, with N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide/N-hydroxysuccinimide as a second crosslinking agent to react with gelatin A and fixed chitosan-gelatin B microspheres to attain improved biocompatibility. Water absorption of the three-dimensional macroporous chitosan-gelatin B microspheres (3D-P-CGMs) was 12.84, with a porosity of 85.45%. In vitro lysozyme degradation after 1, 3, 5, 7, 10, 14, and 21 days showed improved biodegradation in the 3D-P-CGMs. The morphology of human hepatoma cell lines (HepG2 cells) cultured on the 3D-P-CGMs was spherical, unlike that of cells cultured under traditional two-dimensional conditions. Scanning electron microscopy and paraffin sections were used to confirm the porous structure of the 3D-P-CGMs. HepG2 cells were able to migrate inside through the pore. Cell proliferation and levels of albumin and lactate dehydrogenase suggested that the 3D-P-CGMs could provide a larger specific surface area and an appropriate microenvironment for cell growth and survival. Hence, the 3D-P-CGMs are eminently suitable as macroporous scaffolds for cell cultures in tissue engineering and cell carrier studies. Copyright © 2014 John Wiley & Sons, Ltd.