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1.
EMBO J ; 43(10): 2015-2034, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38627599

RESUMEN

Circadian clocks temporally coordinate daily organismal biology over the 24-h cycle. Their molecular design, preserved between fungi and animals, is based on a core-oscillator composed of a one-step transcriptional-translational-negative-feedback-loop (TTFL). To test whether this evolutionarily conserved TTFL architecture is the only plausible way for achieving a functional circadian clock, we adopted a transcriptional rewiring approach, artificially co-opting regulators of the circadian output pathways into the core-oscillator. Herein we describe one of these semi-synthetic clocks which maintains all basic circadian features but, notably, it also exhibits new attributes such as a "lights-on timer" logic, where clock phase is fixed at the end of the night. Our findings indicate that fundamental circadian properties such as period, phase and temperature compensation are differentially regulated by transcriptional and posttranslational aspects of the clockworks.


Asunto(s)
Relojes Circadianos , Transcripción Genética , Relojes Circadianos/genética , Animales , Ritmo Circadiano/genética , Evolución Molecular , Regulación de la Expresión Génica
2.
Cell ; 153(7): 1421-2, 2013 Jun 20.
Artículo en Inglés | MEDLINE | ID: mdl-23791169

RESUMEN

Age-related decline in mammalian circadian rhythm has been recognized for decades, but the underlying molecular mechanisms have remained elusive. In this issue of Cell, Chang and Guarente use brain-specific SIRT1 knockout mice and transgenic mice overexpressing SIRT1 to develop an enticing model for how SIRT1 helps maintain the robustness of the aging circadian clock.


Asunto(s)
Envejecimiento , Relojes Circadianos , Sirtuina 1/metabolismo , Núcleo Supraquiasmático/metabolismo , Animales , Masculino
3.
Mol Cell ; 74(4): 771-784.e3, 2019 05 16.
Artículo en Inglés | MEDLINE | ID: mdl-30954403

RESUMEN

In the negative feedback loop driving fungal and animal circadian oscillators, negative elements (FREQUENCY [FRQ], PERIODS [PERs], and CRYPTOCHROMES [CRYs]) are understood to inhibit their own expression, in part by promoting the phosphorylation of their heterodimeric transcriptional activators (e.g., White Collar-1 [WC-1]-WC-2 [White Collar complex; WCC] and BMAL1/Circadian Locomotor Output Cycles Kaput [CLOCK]). However, correlations between heterodimer activity and phosphorylation are weak, contradictions exist, and mechanistic details are almost wholly lacking. We report mapping of 80 phosphosites on WC-1 and 15 on WC-2 and elucidation of the time-of-day-specific code, requiring both a group of phosphoevents on WC-1 and two distinct clusters on WC-2, that governs circadian repression, leading to feedback loop closure. Combinatorial control via phosphorylation also governs rhythmic WCC binding to the promoters of clock-controlled genes mediating the essential first step in circadian output, a group encoding both transcription factors and signaling proteins. These data provide a basic mechanistic understanding for fundamental events underlying circadian negative feedback and output, key aspects of circadian biology.


Asunto(s)
Ritmo Circadiano/genética , Proteínas de Unión al ADN/genética , Proteínas Fúngicas/genética , Neurospora crassa/genética , Factores de Transcripción/genética , Factores de Transcripción ARNTL/genética , Retroalimentación Fisiológica , Regulación Fúngica de la Expresión Génica , Neurospora crassa/fisiología , Fosforilación , Regiones Promotoras Genéticas/genética , Transducción de Señal/genética
4.
PLoS Biol ; 21(1): e3001961, 2023 01.
Artículo en Inglés | MEDLINE | ID: mdl-36603054

RESUMEN

Compensation is a defining principle of a true circadian clock, where its approximately 24-hour period length is relatively unchanged across environmental conditions. Known compensation effectors directly regulate core clock factors to buffer the oscillator's period length from variables in the environment. Temperature Compensation mechanisms have been experimentally addressed across circadian model systems, but much less is known about the related process of Nutritional Compensation, where circadian period length is maintained across physiologically relevant nutrient levels. Using the filamentous fungus Neurospora crassa, we performed a genetic screen under glucose and amino acid starvation conditions to identify new regulators of Nutritional Compensation. Our screen uncovered 16 novel mutants, and together with 4 mutants characterized in prior work, a model emerges where Nutritional Compensation of the fungal clock is achieved at the levels of transcription, chromatin regulation, and mRNA stability. However, eukaryotic circadian Nutritional Compensation is completely unstudied outside of Neurospora. To test for conservation in cultured human cells, we selected top hits from our fungal genetic screen, performed siRNA knockdown experiments of the mammalian orthologs, and characterized the cell lines with respect to compensation. We find that the wild-type mammalian clock is also compensated across a large range of external glucose concentrations, as observed in Neurospora, and that knocking down the mammalian orthologs of the Neurospora compensation-associated genes CPSF6 or SETD2 in human cells also results in nutrient-dependent period length changes. We conclude that, like Temperature Compensation, Nutritional Compensation is a conserved circadian process in fungal and mammalian clocks and that it may share common molecular determinants.


Asunto(s)
Relojes Circadianos , Neurospora crassa , Nutrientes , Estabilidad del ARN , Humanos , Relojes Circadianos/genética , Ritmo Circadiano/genética , Proteínas Fúngicas/metabolismo , Regulación Fúngica de la Expresión Génica , Glucosa/metabolismo , Neurospora crassa/genética , Neurospora crassa/metabolismo , Estabilidad del ARN/genética , Nutrientes/metabolismo
5.
Mol Cell ; 69(2): 165-168, 2018 01 18.
Artículo en Inglés | MEDLINE | ID: mdl-29276084

RESUMEN

Some longstanding dogmas in the circadian field warrant reexamination in light of recent studies focused on the role of post-translational modifications and intrinsic disorder in core circadian clock proteins of mice and fungi. Such dogmas include the role of turnover in circadian feedback loops and the origin myths describing evolutionary relatedness among circadian clocks. In this Essay, the authors recapitulate recent findings on circadian clock protein regulation by taking an unconventional approach in the form of a dialog between Wizard and Apprentice.


Asunto(s)
Relojes Circadianos/fisiología , Ritmo Circadiano/fisiología , Animales , Evolución Biológica , Proteínas CLOCK , Retroalimentación Fisiológica , Hongos , Humanos , Ratones , Fosforilación , Procesamiento Proteico-Postraduccional/fisiología
6.
J Biol Chem ; : 107508, 2024 Jun 27.
Artículo en Inglés | MEDLINE | ID: mdl-38944116

RESUMEN

In the Neurospora circadian system, the White Collar Complex (WCC) formed by WC-1 and WC-2 drives expression of the frequency (frq) gene whose product FRQ feedbacks to inhibit transcriptional activity of WCC. Phosphorylation of WCC has been extensively studied, but the extent and significance of other post-translational modifications (PTM) has been poorly studied. To this end, we used mass-spectrometry to study alkylation sites on WCC, resulting in discovery of nine acetylation sites. Mutagenesis analysis showed most of the acetylation events individually do not play important roles in period determination. Moreover, mutating all the lysines falling in either half of WC-1 or all the lysine residues in WC-2 to arginines did not abolish circadian rhythms. In addition, we also found nine mono-methylation sites on WC-1, but like acetylation, individual ablation of most of the mono-methylation events did not result in a significant period change. Taken together, the data here suggest that acetylation or mono-methylation on WCC is not a determinant of the pace of the circadian feedback loop. The finding is consistent with a model in which repression of WCC's circadian activity is controlled mainly by phosphorylation. Interestingly, light-induced expression of some light-responsive genes has been modulated in certain wc-1 acetylation mutants, suggesting that WC-1 acetylation events differentially regulate light responses.

7.
Proc Natl Acad Sci U S A ; 119(31): e2203078119, 2022 08 02.
Artículo en Inglés | MEDLINE | ID: mdl-35881801

RESUMEN

The transcription-translation negative feedback loops underlying animal and fungal circadian clocks are remarkably similar in their molecular regulatory architecture and, although much is understood about their central mechanism, little is known about the spatiotemporal dynamics of the gene products involved. A common feature of these circadian oscillators is a significant temporal delay between rhythmic accumulation of clock messenger RNAs (mRNAs) encoding negative arm proteins, for example, frq in Neurospora and Per1-3 in mammals, and the appearance of the clock protein complexes assembled from the proteins they encode. Here, we report use of single-molecule RNA fluorescence in situ hybridization (smFISH) to show that the fraction of nuclei actively transcribing the clock gene frq changes in a circadian manner, and that these mRNAs cycle in abundance with fewer than five transcripts per nucleus at any time. Spatial point patterning statistics reveal that frq is spatially clustered near nuclei in a time of day-dependent manner and that clustering requires an RNA-binding protein, PRD-2 (PERIOD-2), recently shown also to bind to mRNA encoding another core clock component, casein kinase 1. An intrinsically disordered protein, PRD-2 displays behavior in vivo and in vitro consistent with participation in biomolecular condensates. These data are consistent with a role for phase-separating RNA-binding proteins in spatiotemporally organizing clock mRNAs to facilitate local translation and assembly of clock protein complexes.


Asunto(s)
Proteínas CLOCK , Relojes Circadianos , Ritmo Circadiano , Proteínas Fúngicas , Neurospora crassa , Proteínas Circadianas Period , ARN Mensajero , Proteínas CLOCK/genética , Proteínas CLOCK/metabolismo , Relojes Circadianos/genética , Ritmo Circadiano/genética , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Hibridación Fluorescente in Situ , Neurospora crassa/genética , Neurospora crassa/metabolismo , Proteínas Circadianas Period/genética , Proteínas Circadianas Period/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Transcripción Genética
8.
J Biol Chem ; 299(7): 104850, 2023 07.
Artículo en Inglés | MEDLINE | ID: mdl-37220856

RESUMEN

In the negative feedback loop composing the Neurospora circadian clock, the core element, FREQUENCY (FRQ), binds with FRQ-interacting RNA helicase (FRH) and casein kinase 1 to form the FRQ-FRH complex (FFC) which represses its own expression by interacting with and promoting phosphorylation of its transcriptional activators White Collar-1 (WC-1) and WC-2 (together forming the White Collar complex, WCC). Physical interaction between FFC and WCC is a prerequisite for the repressive phosphorylations, and although the motif on WCC needed for this interaction is known, the reciprocal recognition motif(s) on FRQ remains poorly defined. To address this, we assessed FFC-WCC in a series of frq segmental-deletion mutants, confirming that multiple dispersed regions on FRQ are necessary for its interaction with WCC. Biochemical analysis shows that interaction between FFC and WCC but not within FFC or WCC can be disrupted by high salt, suggesting that electrostatic forces drive the association of the two complexes. As a basic sequence on WC-1 was previously identified as a key motif for WCC-FFC assembly, our mutagenetic analysis targeted negatively charged residues of FRQ, leading to identification of three Asp/Glu clusters in FRQ that are indispensable for FFC-WCC formation. Surprisingly, in several frq Asp/Glu-to-Ala mutants that vastly diminish FFC-WCC interaction, the core clock still oscillates robustly with an essentially wildtype period, indicating that the interaction between the positive and negative elements in the feedback loop is required for the operation of the circadian clock but is not a determinant of the period length.


Asunto(s)
Relojes Circadianos , Proteínas Fúngicas , Neurospora crassa , Relojes Circadianos/genética , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Neurospora crassa/efectos de los fármacos , Neurospora crassa/genética , Neurospora crassa/metabolismo , Factores de Transcripción/metabolismo , Dominios Proteicos , Eliminación de Gen , Cloruro de Sodio/farmacología , Mutación , Expresión Génica
9.
Cell ; 137(4): 749-60, 2009 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-19450520

RESUMEN

Temperature compensation of circadian clocks is an unsolved problem with relevance to the general phenomenon of biological compensation. We identify casein kinase 2 (CK2) as a key regulator of temperature compensation of the Neurospora clock by determining that two long-standing clock mutants, chrono and period-3, displaying distinctive alterations in compensation encode the beta1 and alpha subunits of CK2, respectively. Reducing the dose of these subunits, particularly beta1, significantly alters temperature compensation without altering the enzyme's Q(10). By contrast, other kinases and phosphatases implicated in clock function do not play appreciable roles in temperature compensation. CK2 exerts its effects on the clock by directly phosphorylating FREQUENCY (FRQ), and this phosphorylation is compromised in CK2 hypomorphs. Finally, mutation of certain putative CK2 phosphosites on FRQ, shown to be phosphorylated in vivo, predictably alters temperature compensation profiles effectively phenocopying CK2 mutants.


Asunto(s)
Quinasa de la Caseína II/fisiología , Ritmo Circadiano , Neurospora crassa/enzimología , Neurospora crassa/fisiología , Quinasa de la Caseína II/química , Quinasa de la Caseína II/genética , Dosificación de Gen , Mutación , Monoéster Fosfórico Hidrolasas/metabolismo , Fosfotransferasas/metabolismo , Subunidades de Proteína/química , Subunidades de Proteína/genética , Subunidades de Proteína/fisiología , Temperatura
10.
Fungal Genet Biol ; 164: 103763, 2023 01.
Artículo en Inglés | MEDLINE | ID: mdl-36481248

RESUMEN

Fungal cells are quite unique among life in their organization and structure, and yet implementation of many tools recently developed for fluorescence imaging in animal systems and yeast has been slow in filamentous fungi. Here we present analysis of properties of fluorescent proteins in Neurospora crassa as well as describing genetic tools for the expression of these proteins that may be useful beyond cell biology applications. The brightness and photostability of ten different fluorescent protein tags were compared in a well-controlled system; six different promoters are described for the assessment of the fluorescent proteins and varying levels of expression, as well as a customizable bidirectional promoter system. We present an array of fluorescent proteins suitable for use across the visible light spectrum to allow for 4-color imaging, in addition to a photoconvertible fluorescent protein that enables a change in the color of a small subset of proteins in the cell. These tools build on the rich history of cell biology research in filamentous fungi and provide new tools to help expand research capabilities.


Asunto(s)
Neurospora crassa , Animales , Neurospora crassa/genética , Neurospora crassa/metabolismo , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Colorantes/metabolismo
11.
Cell ; 134(2): 212-4, 2008 Jul 25.
Artículo en Inglés | MEDLINE | ID: mdl-18662537

RESUMEN

The transcriptional activator CLOCK is a histone acetyltransferase that is required for the circadian expression of many genes. Asher et al. (2008) and Nakahata et al. (2008) now demonstrate that the NAD(+)-dependent enzyme SIRT1 functions as a histone deacetylase that counteracts the activity of CLOCK. These results broaden our understanding of the impact of cellular metabolism on the circadian system.


Asunto(s)
Ritmo Circadiano , Sirtuinas/metabolismo , Transactivadores/metabolismo , Acetilación , Animales , Proteínas CLOCK , Histona Desacetilasas/metabolismo , Humanos
12.
Fungal Genet Biol ; 156: 103615, 2021 11.
Artículo en Inglés | MEDLINE | ID: mdl-34425213

RESUMEN

Single molecule RNA-FISH (smFISH) is a valuable tool for analysis of mRNA spatial patterning in fixed cells that is underutilized in filamentous fungi. A primary complication for fixed-cell imaging in filamentous fungi is the need for enzymatic cell wall permeabilization, which is compounded by considerable variability in cell wall composition between species. smFISH adds another layer of complexity due to a requirement for RNase free conditions. Here, we describe the cloning, expression, and purification of a chitinase suitable for supplementation of a commercially available RNase-free enzyme preparation for efficient permeabilization of the Neurospora cell wall. We further provide a method for smFISH in Neurospora which includes a tool for generating numerical data from images that can be used in downstream customized analysis protocols.


Asunto(s)
Neurospora crassa , Pared Celular , Digestión , Neurospora crassa/genética , ARN , Ribonucleasas/genética
13.
Mol Cell ; 52(6): 832-43, 2013 Dec 26.
Artículo en Inglés | MEDLINE | ID: mdl-24316221

RESUMEN

Protein conformation dictates a great deal of protein function. A class of naturally unstructured proteins, termed intrinsically disordered proteins (IDPs), demonstrates that flexibility in structure can be as important mechanistically as rigid structure. At the core of the circadian transcription/translation feedback loop in Neurospora crassa is the protein FREQUENCY (FRQ), shown here shown to share many characteristics of IDPs. FRQ in turn binds to FREQUENCY-Interacting RNA Helicase (FRH), whose clock function has been assumed to relate to its predicted helicase function. However, mutational analyses reveal that the helicase function of FRH is not essential for the clock, and a region of FRH distinct from the helicase region is essential for stabilizing FRQ against rapid degradation via a pathway distinct from its typical ubiquitin-mediated turnover. These data lead to the hypothesis that FRQ is an IDP and that FRH acts nonenzymatically, stabilizing FRQ to enable proper clock circuitry/function.


Asunto(s)
Proteínas CLOCK/metabolismo , Ritmo Circadiano , Proteínas Fúngicas/metabolismo , Proteínas Intrínsecamente Desordenadas/metabolismo , Neurospora crassa/enzimología , ARN Helicasas/metabolismo , Proteínas CLOCK/genética , Ritmo Circadiano/genética , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas Fúngicas/genética , Genotipo , Proteínas Intrínsecamente Desordenadas/genética , Mutación , Neurospora crassa/genética , Neurospora crassa/crecimiento & desarrollo , Fenotipo , Unión Proteica , Dominios y Motivos de Interacción de Proteínas , Estabilidad Proteica , Proteolisis , ARN Helicasas/genética , Proteínas Recombinantes/metabolismo , Factores de Tiempo
14.
PLoS Genet ; 14(1): e1007192, 2018 01.
Artículo en Inglés | MEDLINE | ID: mdl-29351294

RESUMEN

Circadian clocks are ubiquitous in eukaryotic organisms where they are used to anticipate regularly occurring diurnal and seasonal environmental changes. Nevertheless, little is known regarding pathways connecting the core clock to its output pathways. Here, we report that the HAD family phosphatase CSP-6 is required for overt circadian clock output but not for the core oscillation. The loss of function Δcsp-6 deletion mutant is overtly arrhythmic on race tubes under free running conditions; however, reporter assays confirm that the FREQUENCY-WHITE COLLAR COMPLEX core circadian oscillator is functional, indicating a discrete block between oscillator and output. CSP-6 physically interacts with WHI-2, Δwhi-2 mutant phenotypes resemble Δcsp-6, and the CSP-6/WHI-2 complex physically interacts with WC-1, all suggesting that WC-1 is a direct target for CSP-6/WHI-2-mediated dephosphorylation and consistent with observed WC-1 hyperphosphorylation in Δcsp-6. To identify the source of the block to output, known clock-controlled transcription factors were screened for rhythmicity in Δcsp-6, identifying loss of circadian control of ADV-1, a direct target of WC-1, as responsible for the loss of overt rhythmicity. The CSP-6/WHI-2 complex thus participates in the clock output pathway by regulating WC-1 phosphorylation to promote proper transcriptional/translational activation of adv-1/ADV-1; these data establish an unexpected essential role for post-translational modification parallel to circadian transcriptional regulation in the early steps of circadian output.


Asunto(s)
Ritmo Circadiano/genética , Proteínas Fúngicas/fisiología , Hidrolasas/fisiología , Neurospora crassa/genética , Monoéster Fosfórico Hidrolasas/fisiología , Relojes Circadianos/genética , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Proteínas de Unión al ADN/fisiología , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Regulación Fúngica de la Expresión Génica , Hidrolasas/genética , Neurospora crassa/enzimología , Organismos Modificados Genéticamente , Fosforilación , Unión Proteica , Transducción de Señal/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Factores de Transcripción/fisiología
15.
Trends Biochem Sci ; 41(10): 834-846, 2016 10.
Artículo en Inglés | MEDLINE | ID: mdl-27498225

RESUMEN

From cyanobacteria to mammals, organisms have evolved timing mechanisms to adapt to environmental changes in order to optimize survival and improve fitness. To anticipate these regular daily cycles, many organisms manifest ∼24h cell-autonomous oscillations that are sustained by transcription-translation-based or post-transcriptional negative-feedback loops that control a wide range of biological processes. With an eye to identifying emerging common themes among cyanobacterial, fungal, and animal clocks, some major recent developments in the understanding of the mechanisms that regulate these oscillators and their output are discussed. These include roles for antisense transcription, intrinsically disordered proteins, codon bias in clock genes, and a more focused discussion of post-transcriptional and translational regulation as a part of both the oscillator and output.


Asunto(s)
Péptidos y Proteínas de Señalización del Ritmo Circadiano/genética , Ritmo Circadiano/genética , Retroalimentación Fisiológica , Biosíntesis de Proteínas , Procesamiento Proteico-Postraduccional , Transcripción Genética , Animales , Ritmo Circadiano/efectos de la radiación , Péptidos y Proteínas de Señalización del Ritmo Circadiano/metabolismo , Codón , Secuencia Conservada , Cianobacterias/genética , Cianobacterias/metabolismo , Cianobacterias/efectos de la radiación , Hongos/genética , Hongos/metabolismo , Hongos/efectos de la radiación , Interacción Gen-Ambiente , Humanos , Proteínas Intrínsecamente Desordenadas/genética , Proteínas Intrínsecamente Desordenadas/metabolismo , Luz , Fototransducción
16.
EMBO J ; 35(15): 1707-19, 2016 08 01.
Artículo en Inglés | MEDLINE | ID: mdl-27340124

RESUMEN

In the Neurospora crassa circadian clock, a protein complex of frequency (FRQ), casein kinase 1a (CK1a), and the FRQ-interacting RNA Helicase (FRH) rhythmically represses gene expression by the white-collar complex (WCC). FRH crystal structures in several conformations and bound to ADP/RNA reveal differences between FRH and the yeast homolog Mtr4 that clarify the distinct role of FRH in the clock. The FRQ-interacting region at the FRH N-terminus has variable structure in the absence of FRQ A known mutation that disrupts circadian rhythms (R806H) resides in a positively charged surface of the KOW domain, far removed from the helicase core. We show that changes to other similarly located residues modulate interactions with the WCC and FRQ A V142G substitution near the N-terminus also alters FRQ and WCC binding to FRH, but produces an unusual short clock period. These data support the assertion that FRH helicase activity does not play an essential role in the clock, but rather FRH acts to mediate contacts among FRQ, CK1a and the WCC through interactions involving its N-terminus and KOW module.


Asunto(s)
Relojes Circadianos , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Neurospora crassa/enzimología , ARN Helicasas/química , ARN Helicasas/metabolismo , Cristalografía por Rayos X , Proteínas Fúngicas/genética , Modelos Moleculares , Proteínas Mutantes/química , Proteínas Mutantes/genética , Unión Proteica , Conformación Proteica , Dominios Proteicos , ARN Helicasas/genética
17.
Bioinformatics ; 35(9): 1518-1526, 2019 05 01.
Artículo en Inglés | MEDLINE | ID: mdl-30247517

RESUMEN

MOTIVATION: Decreasing costs are making it feasible to perform time series proteomics and genomics experiments with more replicates and higher resolution than ever before. With more replicates and time points, proteome and genome-wide patterns of expression are more readily discernible. These larger experiments require more batches exacerbating batch effects and increasing the number of bias trends. In the case of proteomics, where methods frequently result in missing data this increasing scale is also decreasing the number of peptides observed in all samples. The sources of batch effects and missing data are incompletely understood necessitating novel techniques. RESULTS: Here we show that by exploiting the structure of time series experiments, it is possible to accurately and reproducibly model and remove batch effects. We implement Learning and Imputation for Mass-spec Bias Reduction (LIMBR) software, which builds on previous block-based models of batch effects and includes features specific to time series and circadian studies. To aid in the analysis of time series proteomics experiments, which are often plagued with missing data points, we also integrate an imputation system. By building LIMBR for imputation and time series tailored bias modeling into one straightforward software package, we expect that the quality and ease of large-scale proteomics and genomics time series experiments will be significantly increased. AVAILABILITY AND IMPLEMENTATION: Python code and documentation is available for download at https://github.com/aleccrowell/LIMBR and LIMBR can be downloaded and installed with dependencies using 'pip install limbr'. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.


Asunto(s)
Programas Informáticos , Genoma , Genómica , Espectrometría de Masas , Proteómica
18.
Cell Commun Signal ; 18(1): 181, 2020 11 11.
Artículo en Inglés | MEDLINE | ID: mdl-33176800

RESUMEN

INTRODUCTION: The circadian circuit, a roughly 24 h molecular feedback loop, or clock, is conserved from bacteria to animals and allows for enhanced organismal survival by facilitating the anticipation of the day/night cycle. With circadian regulation reportedly impacting as high as 80% of protein coding genes in higher eukaryotes, the protein-based circadian clock broadly regulates physiology and behavior. Due to the extensive interconnection between the clock and other cellular systems, chronic disruption of these molecular rhythms leads to a decrease in organismal fitness as well as an increase of disease rates in humans. Importantly, recent research has demonstrated that proteins comprising the circadian clock network display a significant amount of intrinsic disorder. MAIN BODY: In this work, we focus on the extent of intrinsic disorder in the circadian clock and its potential mechanistic role in circadian timing. We highlight the conservation of disorder by quantifying the extent of computationally-predicted protein disorder in the core clock of the key eukaryotic circadian model organisms Drosophila melanogaster, Neurospora crassa, and Mus musculus. We further examine previously published work, as well as feature novel experimental evidence, demonstrating that the core negative arm circadian period drivers FREQUENCY (Neurospora crassa) and PERIOD-2 (PER2) (Mus musculus), possess biochemical characteristics of intrinsically disordered proteins. Finally, we discuss the potential contributions of the inherent biophysical principals of intrinsically disordered proteins that may explain the vital mechanistic roles they play in the clock to drive their broad evolutionary conservation in circadian timekeeping. CONCLUSION: The pervasive conservation of disorder amongst the clock in the crown eukaryotes suggests that disorder is essential for optimal circadian timing from fungi to animals, providing vital homeostatic cellular maintenance and coordinating organismal physiology across phylogenetic kingdoms. Video abstract.


Asunto(s)
Ritmo Circadiano/fisiología , Proteínas Intrínsecamente Desordenadas/metabolismo , Animales , Relojes Circadianos , Humanos , Proteínas Intrínsecamente Desordenadas/química , Conformación Proteica
19.
Mol Ecol ; 27(1): 216-232, 2018 01.
Artículo en Inglés | MEDLINE | ID: mdl-29134709

RESUMEN

Understanding the genetic basis of the switch from asexual to sexual lifestyles in response to sometimes rapid environmental changes is one of the major challenges in fungal ecology. Light appears to play a critical role in the asexual-sexual switch-but fungal genomes harbour diverse light sensors. Fungal opsins are homologous to bacterial green-light-sensory rhodopsins, and their organismal functions in fungi have not been well understood. Three of these opsin-like proteins were widely distributed across fungal genomes, but homologs of the Fusarium opsin-like protein CarO were present only in plant-associated fungi. Key amino acids, including potential retinal binding sites, functionally diverged on the phylogeny of opsins. This diversification of opsin-like proteins could be correlated with life history-associated differences among fungi in their expression and function during morphological development. In Neurospora crassa and related species, knockout of the opsin NOP-1 led to a phenotype in the regulation of the asexual-sexual switch, modulating response to both light and oxygen conditions. Sexual development commenced early in ∆nop-1 strains cultured in unsealed plates under constant blue and white light. Furthermore, comparative transcriptomics showed that the expression of nop-1 is light-dependent and that the ∆nop-1 strain abundantly expresses genes involved in oxidative stress response, genes enriched in NAD/NADP binding sites, genes with functions in proton transmembrane movement and catalase activity, and genes involved in the homeostasis of protons. Based on these observations, we contend that light and oxidative stress regulate the switch via light-responsive and ROS pathways in model fungus N. crassa and other fungi.


Asunto(s)
Fenómenos Ecológicos y Ambientales , Proteínas Fúngicas/metabolismo , Luz , Neurospora crassa/fisiología , Opsinas/metabolismo , Secuencia de Aminoácidos , Secuencia Conservada , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Regulación Fúngica de la Expresión Génica , Técnicas de Inactivación de Genes , Redes Reguladoras de Genes , Genes Fúngicos , Modelos Biológicos , Complejos Multienzimáticos/metabolismo , NADH NADPH Oxidorreductasas/metabolismo , Neurospora crassa/genética , Neurospora crassa/crecimiento & desarrollo , Neurospora crassa/efectos de la radiación , Oxidación-Reducción , Estrés Oxidativo/genética , Fenotipo , Filogenia , Estructura Secundaria de Proteína , Reproducción/efectos de la radiación , Regulación hacia Arriba/genética
20.
Appl Microbiol Biotechnol ; 102(9): 3849-3863, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-29569180

RESUMEN

Regulatable promoters are important genetic tools, particularly for assigning function to essential and redundant genes. They can also be used to control the expression of enzymes that influence metabolic flux or protein secretion, thereby optimizing product yield in bioindustry. This review will focus on regulatable systems for use in filamentous fungi, an important group of organisms whose members include key research models, devastating pathogens of plants and animals, and exploitable cell factories. Though we will begin by cataloging those promoters that are controlled by nutritional or chemical means, our primary focus will rest on those who can be controlled by a literal flip-of-the-switch: promoters of light-regulated genes. The vvd promoter of Neurospora will first serve as a paradigm for how light-driven systems can provide tight, robust, tunable, and temporal control of either autologous or heterologous fungal proteins. We will then discuss a theoretical approach to, and practical considerations for, the development of such promoters in other species. To this end, we have compiled genes from six previously published light-regulated transcriptomic studies to guide the search for suitable photoregulatable promoters in your fungus of interest.


Asunto(s)
Proteínas Fúngicas/genética , Regulación Fúngica de la Expresión Génica/efectos de la radiación , Genes Fúngicos/genética , Luz , Neurospora crassa/genética , Neurospora crassa/efectos de la radiación , Regiones Promotoras Genéticas/genética
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