RESUMEN
Alteration in the buffering capacity of the proteostasis network is an emerging feature of Alzheimer's disease (AD), highlighting the occurrence of endoplasmic reticulum (ER) stress. The unfolded protein response (UPR) is the main adaptive pathway to cope with protein folding stress at the ER. Inositol-requiring enzyme-1 (IRE1) operates as a central ER stress sensor, enabling the establishment of adaptive and repair programs through the control of the expression of the transcription factor X-box binding protein 1 (XBP1). To artificially enforce the adaptive capacity of the UPR in the AD brain, we developed strategies to express the active form of XBP1 in the brain. Overexpression of XBP1 in the nervous system using transgenic mice reduced the load of amyloid deposits and preserved synaptic and cognitive function. Moreover, local delivery of XBP1 into the hippocampus of an 5xFAD mice using adeno-associated vectors improved different AD features. XBP1 expression corrected a large proportion of the proteomic alterations observed in the AD model, restoring the levels of several synaptic proteins and factors involved in actin cytoskeleton regulation and axonal growth. Our results illustrate the therapeutic potential of targeting UPR-dependent gene expression programs as a strategy to ameliorate AD features and sustain synaptic function.
Asunto(s)
Enfermedad de Alzheimer , Animales , Ratones , Enfermedad de Alzheimer/genética , Enfermedad de Alzheimer/terapia , Enfermedad de Alzheimer/metabolismo , Estrés del Retículo Endoplásmico/genética , Ratones Transgénicos , Proteómica , Proteostasis/genética , Transducción de Señal/fisiología , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Respuesta de Proteína Desplegada/genéticaRESUMEN
Altered proteostasis is a salient feature of Alzheimer's disease (AD), highlighting the occurrence of endoplasmic reticulum (ER) stress and abnormal protein aggregation. ER stress triggers the activation of the unfolded protein response (UPR), a signaling pathway that enforces adaptive programs to sustain proteostasis or eliminate terminally damaged cells. IRE1 is an ER-located kinase and endoribonuclease that operates as a major stress transducer, mediating both adaptive and proapoptotic programs under ER stress. IRE1 signaling controls the expression of the transcription factor XBP1, in addition to degrade several RNAs. Importantly, a polymorphism in the XBP1 promoter was suggested as a risk factor to develop AD. Here, we demonstrate a positive correlation between the progression of AD histopathology and the activation of IRE1 in human brain tissue. To define the significance of the UPR to AD, we targeted IRE1 expression in a transgenic mouse model of AD. Despite initial expectations that IRE1 signaling may protect against AD, genetic ablation of the RNase domain of IRE1 in the nervous system significantly reduced amyloid deposition, the content of amyloid ß oligomers, and astrocyte activation. IRE1 deficiency fully restored the learning and memory capacity of AD mice, associated with improved synaptic function and improved long-term potentiation (LTP). At the molecular level, IRE1 deletion reduced the expression of amyloid precursor protein (APP) in cortical and hippocampal areas of AD mice. In vitro experiments demonstrated that inhibition of IRE1 downstream signaling reduces APP steady-state levels, associated with its retention at the ER followed by proteasome-mediated degradation. Our findings uncovered an unanticipated role of IRE1 in the pathogenesis of AD, offering a novel target for disease intervention.
Asunto(s)
Enfermedad de Alzheimer/metabolismo , Hipocampo/metabolismo , Proteínas de la Membrana/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Transducción de Señal/fisiología , Enfermedad de Alzheimer/genética , Enfermedad de Alzheimer/patología , Péptidos beta-Amiloides/metabolismo , Precursor de Proteína beta-Amiloide/metabolismo , Animales , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Estrés del Retículo Endoplásmico/fisiología , Hipocampo/patología , Humanos , Potenciación a Largo Plazo/fisiología , Proteínas de la Membrana/genética , Ratones , Ratones Transgénicos , Neuronas/metabolismo , Neuronas/patología , Proteínas Serina-Treonina Quinasas/genética , Memoria Espacial/fisiología , Respuesta de Proteína Desplegada/fisiologíaRESUMEN
For a deeper understanding of the phylogenetic relationships of Echinococcus genotypes and species in different intermediate hosts, we analyzed samples from human and bovine hydatid cysts. For this, segments of the cytochrome oxidase (COX1) and NADH dehydrogenase (ND1) mitochondrial genes were used. To obtain sufficient amounts of the ND1 marker to be sequenced properly, a new variant of the PCR assay was implemented. Phylogenetic analysis with both markers showed that most of the analyzed samples correspond to genotype G1. However, a sample from cysts of a bovine lung (Q21), with the COX1 marker, was grouped in a node together with a sample belonging to genotype G3. In the phylogenetic tree obtained with the ND1 marker, this sample was grouped with sequences of genotypes G3, G2, and G4. Analyzing the single nucleotide polymorphic (SNP) sites of both markers, it was observed that the Q21 sequence is almost identical to the G3 sequence and differ in only one SNP from the G2 sequence, and is completely different from G4. These results are noteworthy, since neither G2 nor G3 genotypes have been described previously in Chile, raising the possibility that the G3 genotype is present in these latitudes. This information is highly relevant; it can be employed to uncover additional unknown details of transmission cycles of this important parasite.
Asunto(s)
Bovinos/parasitología , Equinococosis/veterinaria , Echinococcus granulosus/clasificación , Genotipo , Animales , Chile , ADN de Helmintos/genética , Equinococosis/parasitología , Echinococcus granulosus/genética , Complejo IV de Transporte de Electrones/genética , Genes Mitocondriales , Marcadores Genéticos , NADH Deshidrogenasa/genética , Filogenia , Reacción en Cadena de la Polimerasa/veterinaria , Polimorfismo de Nucleótido SimpleRESUMEN
BACKGROUND: Hydatid disease or cystic echinococcosis, caused by the parasite Echinococcus granulosus, has a worldwide distribution, affecting people of working age and can cause high levels of morbidity and even death. AIM: To estimate the economic impact at the human and animal level caused by the disease in Chile. MATERIAL AND METHODS: We analyzed information about the disease obtained from reports and publications emanated from the Chilean Ministry of Health, United Nations Food and Agriculture Organization, the U.S. National Institute of Statistics and the National Agricultural Service. Animal derived costs were estimated evaluating the expenses for pharmacological treatment of infected dogs and animal production losses derived from confiscations and reductions in meat production. RESULTS: The total number of patients who underwent surgery to remove a hydatid cyst in Chile during 2012, was estimated as 767 individuals. The annual costs derived only from surgical treatment, were estimated in USD 2.46 million. Summing the costs of sick leaves and loss of productivity, the costs at the human level ascended to USD 3.13 million. Considering human and animal costs, the annual economic burden of the disease was estimated in USD 14.35 million. CONCLUSIONS: The Analysis of the regional distribution of human and animal hydatidosis, suggests a significant environmental contamination with parasite eggs in high incidence regions such as Aysén, Araucanía, BioBío and Coquimbo. The efficiency of control programs for the disease would be greatly improved if the causes for these regional contaminations are elucidated.
Asunto(s)
Costo de Enfermedad , Equinococosis/economía , Costos de la Atención en Salud , Crianza de Animales Domésticos/economía , Animales , Chile/epidemiología , Enfermedades de los Perros/tratamiento farmacológico , Enfermedades de los Perros/economía , Enfermedades de los Perros/epidemiología , Perros , Equinococosis/epidemiología , Equinococosis/terapia , Equinococosis/veterinaria , Humanos , Incidencia , Ausencia por Enfermedad/economíaRESUMEN
Research has shown that children of undocumented Latinx parents in the United States are at greater risk for negative long-term effects on their mental health and overall well-being. Chief among these concerns are the negative effects of disrupted attachment processes, as deported parents are often taken from their families by force and required to parent from afar, if they can continue parenting at all. Despite the ubiquity of deported families, little is known about the effects of deportation on the attachment of left-behind children and the subsequent potential disruptive effect of deportation on their adult relationships. This phenomenological study aims to understand how adults who have experienced parental deportation in their childhood describe the effects of that event on their adult intimate relationships. Themes of (1) ambiguous loss; (2) inability to trust others; (3) fear of separation from loved ones; and (4) shame emerged and are discussed considering existing literature on attachment theory, immigration, and the Latinx population. Treatment implications are also discussed.
Asunto(s)
Deportación , Emigración e Inmigración , Adulto , Niño , Humanos , Estados Unidos , Padres/psicología , Responsabilidad Parental , Salud MentalRESUMEN
Insulin-like growth factor-1 (IGF-1) signaling is a key pathway in the control of cell growth and survival. Three critical nodes in the IGF-1 signaling pathway have been described in cardiomyocytes: protein kinase Akt/mammalian target of rapamycin (mTOR), Ras/Raf/extracellular signal-regulated kinase (ERK), and phospholipase C (PLC)/inositol 1,4,5-triphosphate (InsP3 )/Ca(2+) . The Akt/mTOR and Ras/Raf/ERK signaling arms govern survival in the settings of cardiac stress and hypertrophic growth. By contrast, PLC/InsP3 /Ca(2+) functions to regulate metabolic adaptability and gene transcription. Autophagy is a catabolic process involved in protein degradation, organelle turnover, and nonselective breakdown of cytoplasmic components during nutrient starvation or stress. In the heart, autophagy is observed in a variety of human pathologies, where it can be either adaptive or maladaptive, depending on the context. We proposed the hypothesis that IGF-1 protects the heart by rescuing the mitochondrial metabolism and the energetics state, reducing cell death and controls the potentially exacerbate autophagic response to nutritional stress. In light of the importance of IGF-1 and autophagy in the heart, we review here IGF-1 signaling and autophagy regulation in the context of cardiomyocyte nutritional stress.
Asunto(s)
Factor I del Crecimiento Similar a la Insulina/metabolismo , Miocardio/metabolismo , Miocitos Cardíacos/metabolismo , Estrés Fisiológico , Autofagia , Proliferación Celular , Humanos , Mitocondrias/metabolismo , Miocitos Cardíacos/fisiología , Transducción de SeñalRESUMEN
The objective of the trial was to evaluate three vaccination schemes against Clostridium perfringens (CP) alpha-toxoid through drinking water to determine if they can protect against clinical signs of necrotic enteritis and coccidiosis in broiler chickens. Three hundred 1-day-old Cobb 500 male chicks were used in 4 treatments with 10 repetitions. Each group received 1 of the following treatments over the course of 29 days: T1, no vaccination; T2, vaccination on Day 1; T3, vaccination on Day 7; and T4, vaccination on Days 7 and 17. The birds were vaccinated with inactivated CP toxoid type A, administered via drinking water. During the first 14 days, a high-protein diet (27%) consisting of corn, soy, and fish meal was fed. On Day 14 Eimeria acervulina (EA), Eimeria maxima (EMx), Eimeria tenella (ET), Eimeria necatrix, and Eimeria brunetti were used in a coccidial challenge. The field isolate CP type A was then inoculated on Days 18, 19, and 20. Ten birds were slaughtered by treatment to obtain serology samples for antibody titers and intestine samples for CP and Eimeria lesion score and gut integrity indicators. Productive performance was assessed using complete randomized design and compared statistically using the Tukey test, whereas intestinal integrity variables and antibodies against CP alpha toxin were assessed using a Kruskal-Wallis nonparametric method. The results revealed that the treatments had an effect on productive performance (P < 0.05); T3 had better body weight and weight gain than T1. In terms of lesion score at Day 21, T4 had a lower lesion score by EA, EMx, and ET than T1. Cell desquamation in T2 was lower than in T4, and excess mucus (EM) in T1 was the worst in gut integrity indicators at Day 21. On the other hand, T2 had more EM than T3 and T4 at Day 25. In the measurement of antibodies, no statistical differences (P > 0.05) were found. These findings indicate that vaccination on Day 7 (T3) outperformed double vaccination on Days 7 and 17 (T4) and single on Day 1 (T2), in terms of productive performance, gut integrity, and lesion scores; and on the last day of the experiment T3 had the best performance in immunology response.
Evaluación de tres esquemas de vacunación contra la toxina alfa de Clostridium perfringens y sus efectos sobre el rendimiento, el nivel de lesiones intestinales y los títulos de anticuerpos séricos en pollos de engorde. El objetivo del ensayo fue evaluar tres programas de vacunación contra Clostridium perfringens (CP) con un alfa-toxoide a través del agua de bebida para determinar si protegían contra signos clínicos de enteritis necrótica y coccidiosis en pollos de engorde. Para ello se emplearon 300 pollitos machos Cobb 500 de un día de edad, distribuidos en 4 tratamientos con 10 repeticiones. Cada grupo recibió, durante 29 días, uno de los siguientes tratamientos: T1: sin vacunación; T2: vacunación en el día uno; T3: vacunación en el día siete y T4, vacunación en los días siete y 17. Las aves fueron vacunadas con toxoide inactivado de C. perfringens tipo A, que se administró en el agua de bebida. Durante los primeros 14 días se alimentó con una dieta alta en proteína (27%) que consistía en maíz, soya y harina de pescado. El desafío coccidial se realizó en el día 14 con Eimeria acervulina (EA), Eimeria maxima (EMx), Eimeria tenella (ET), Eimeria necatrix and Eimeria brunetti. Posteriormente, en los días 18, 19 y 20 se inoculó una cepa aislada de campo de C. perfringens tipo A. Se sacrificaron diez aves por tratamiento para obtener muestras de sueros para determinar los títulos de anticuerpos y muestras de intestino para determinar la puntuación de lesiones por C. perfringens, por Eimeria y los indicadores de integridad intestinal. El comportamiento productivo se analizó bajo un diseño completamente al azar (DCA) y la comparación estadística se realizó mediante la prueba de Tukey, mientras que para las variables de integridad intestinal y los títulos de anticuerpos contra alfa toxina de C. perfringens se utilizó el método no paramétrico Kruskal-Wallis. Los resultados mostraron que el comportamiento productivo fue influenciado por los tratamientos (P < 0.05); el tratamiento T3 mostró el mejor peso corporal y ganancia de peso en comparación con el tratamiento T1. Con relación al puntaje de lesiones en el día 21, el tratamiento T4 tuvo el menor puntaje de lesiones por E. acervulina, E. maxima y E. tenella en comparación con el tratamiento T1. La descamación celular en el tratamiento T2 fue menor que en el T4 y el exceso de moco en el tratamiento T1 fue peor entre los indicadores de integridad intestinal en el día 21. Por otro lado, el tratamiento T2 tenía más exceso de moco en comparación con los tratamientos T3 y T4 en el día 25. No se encontraron diferencias estadísticas (P > 0.05) en la medición de títulos de anticuerpos. Estos hallazgos indican que la vacunación en el día siete (T3) superó a la vacunación doble en los días 7 y 17 (T4) y única en el día uno (T2), en términos de rendimiento productivo, integridad intestinal y puntajes de lesiones, además en el último día del experimento, el tratamiento T3 tuvo el mejor desempeño en la respuesta inmunológica.
Asunto(s)
Infecciones por Clostridium , Coccidiosis , Agua Potable , Eimeria tenella , Eimeria , Enteritis , Enfermedades de las Aves de Corral , Animales , Masculino , Alimentación Animal/análisis , Pollos , Infecciones por Clostridium/prevención & control , Infecciones por Clostridium/veterinaria , Clostridium perfringens/fisiología , Coccidiosis/prevención & control , Coccidiosis/veterinaria , Dieta/veterinaria , Eimeria/fisiología , Enteritis/prevención & control , Enteritis/veterinaria , Enfermedades de las Aves de Corral/prevención & control , ToxoidesRESUMEN
Alzheimer's disease (AD) is the most common neurodegenerative disorder, characterized by protein accumulation in the brain as a main neuropathological hallmark. Among them, Aß42 peptides tend to aggregate and create oligomers and plaques. Macroautophagy, a form of autophagy characterized by a double-membrane vesicle, plays a crucial role in maintaining neuronal homeostasis by degrading protein aggregates and dysfunctional organelles as a quality control process. Recently, DEF8, a relatively uncharacterized protein, has been proposed as a participant in vesicular traffic and autophagy pathways. We have reported increased DEF8 levels in lymphocytes from mild cognitive impairment (MCI) and early-stage AD patients and a neuronal profile in a murine transgenic AD model. Here, we analyzed DEF8 localization and levels in the postmortem frontal cortex of AD patients, finding increased levels compared to healthy controls. To evaluate the potential function of DEF8 in the nervous system, we performed an in silico assessment of its expression and network profiles, followed by an in vivo evaluation of a neuronal Def8 deficient model using a Drosophila melanogaster model of AD based on Aß42 expression. Our findings show that DEF8 is an essential protein for maintaining cellular homeostasis in the nervous system, and it is upregulated under stress conditions generated by Aß42 aggregation. This study suggests DEF8 as a novel actor in the physiopathology of AD, and its exploration may lead to new treatment avenues.
Asunto(s)
Enfermedad de Alzheimer , Animales , Humanos , Enfermedad de Alzheimer/metabolismo , Péptidos beta-Amiloides/metabolismo , Autofagia/genética , Encéfalo/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Fragmentos de Péptidos/metabolismoRESUMEN
In this article, we present partial findings from a thematic analysis study that examined integrating emotionally focused therapy (EFT) and eye-movement desensitization and reprocessing (EMDR) as clinical frameworks in couple therapy. The purpose of the study is to better understand how therapists integrate EFT and EMDR therapy in their clinical work. Thirteen licensed therapists (n = 13) trained in EFT and EMDR were interviewed about their experiences integrating these two models in their couple therapy practice. The findings included in this article are related to how these models complement each other as well as the clinical benefits associated with their integration. Findings provide preliminary evidence that there are benefits and challenges when integrating both models, although we emphasize complementarity in this article. Limitations and implications for future research on the integration and efficacy of these two models are also discussed.
Asunto(s)
Terapia de Parejas , Terapia Centrada en la Emoción , Desensibilización y Reprocesamiento del Movimiento Ocular , HumanosRESUMEN
Alzheimer's disease (AD) is the most prevalent age-associated neurodegenerative disease. A decrease in autophagy during aging contributes to brain disorders by accumulating potentially toxic substrates in neurons. Rubicon is a well-established inhibitor of autophagy in all cells. However, Rubicon participates in different pathways depending on cell type, and little information is currently available on neuronal Rubicon's role in the AD context. Here, we investigated the cell-specific expression of Rubicon in postmortem brain samples from AD patients and 5xFAD mice and its impact on amyloid ß burden in vivo and neuroblastoma cells. Further, we assessed Rubicon levels in human-induced pluripotent stem cells (hiPSCs), derived from early-to-moderate AD and in postmortem samples from severe AD patients. We found increased Rubicon levels in AD-hiPSCs and postmortem samples and a notable Rubicon localization in neurons. In AD transgenic mice lacking Rubicon, we observed intensified amyloid ß burden in the hippocampus and decreased Pacer and p62 levels. In APP-expressing neuroblastoma cells, increased APP/amyloid ß secretion in the medium was found when Rubicon was absent, which was not observed in cells depleted of Atg5, essential for autophagy, or Rab27a, required for exosome secretion. Our results propose an uncharacterized role of Rubicon on APP/amyloid ß homeostasis, in which neuronal Rubicon is a repressor of APP/amyloid ß secretion, defining a new way to target AD and other similar diseases therapeutically.
Asunto(s)
Enfermedad de Alzheimer , Proteínas Relacionadas con la Autofagia , Neuroblastoma , Enfermedades Neurodegenerativas , Enfermedad de Alzheimer/metabolismo , Péptidos beta-Amiloides/metabolismo , Precursor de Proteína beta-Amiloide/metabolismo , Animales , Proteínas Relacionadas con la Autofagia/metabolismo , Humanos , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Ratones , Ratones Transgénicos , Neuroblastoma/metabolismo , Enfermedades Neurodegenerativas/metabolismo , Neuronas/metabolismoRESUMEN
BACKGROUND: Disturbances in the autophagy/endolysosomal systems are proposed as early signatures of Alzheimer's disease (AD). However, few studies are available concerning autophagy gene expression in AD patients. OBJECTIVE: To explore the differential expression of classical genes involved in the autophagy pathway, among them a less characterized one, DEF8 (Differentially expressed in FDCP 8), initially considered a Rubicon family member, in peripheralblood mononuclear cells (PBMCs) from individuals with mild cognitive impairment (MCI) and probable AD (pAD) and correlate the results with the expression of DEF8 in the brain of 5xFAD mice. METHOD: By real-time PCR and flow cytometry, we evaluated autophagy genes levels in PBMCs from MCI and pAD patients. We evaluated DEF8 levels and its localization in brain samples of the 5xFAD mice by real-time PCR, western blot, and immunofluorescence. RESULTS: Transcriptional levels of DEF8 were significantly reduced in PBMCs of MCI and pAD patients compared with healthy donors, correlating with the MoCA and MoCA-MIS cognitive tests scores. DEF8 protein levels were increased in lymphocytes from MCI but not pAD, compared to controls. In the case of brain samples from 5xFAD mice, we observed a reduced mRNA expression and augmented protein levels in 5xFAD compared to age-matched wild-type mice. DEF8 presented a neuronal localization. CONCLUSION: DEF8, a protein proposed to act at the final step of the autophagy/endolysosomal pathway, is differentially expressed in PBMCs of MCI and pAD and neurons of 5xFAD mice. These results suggest a potential role for DEF8 in the pathophysiology of AD.
Asunto(s)
Enfermedad de Alzheimer/metabolismo , Autofagia/fisiología , Encéfalo/metabolismo , Disfunción Cognitiva/metabolismo , Péptidos y Proteínas de Señalización Intracelular/fisiología , Anciano , Anciano de 80 o más Años , Enfermedad de Alzheimer/genética , Enfermedad de Alzheimer/patología , Animales , Biomarcadores/metabolismo , Encéfalo/patología , Disfunción Cognitiva/genética , Disfunción Cognitiva/patología , Femenino , Humanos , Péptidos y Proteínas de Señalización Intracelular/genética , Masculino , Ratones , Ratones Transgénicos , Persona de Mediana EdadRESUMEN
El presente trabajo tiene como objetivo, caracterizar la violenciaen pareja en personas con seropositividad (prevalencia,tipos de acciones violentas, variables de riesgo/ protectorasy consecuencias), para lo cual se realizó una revisión sistemáticaa través del protocolo PRISMA. La búsqueda de artículos serealizó en Scopus, Web of Science, Eric, Scielo y Pubmedpublicados hasta 2021. Se encontraron 113 artículos, de loscuales 22 cumplieron con los criterios de elegibilidad. Losresultados indican que la violencia en pareja en personasseropositivas, además de manifestarse de manera física,psicológica, patrimonial, sexual y verbal, se puede presentar através del uso del VIH para ejercer la violencia. La prevalenciavaría en función del contexto geográfico en un intervalo del19,6% al 43,1%; la cual es superior en población migrantey en hombres que tienen sexo con hombres (HSH) mujeresseropositivas, parejas no heterosexuales y en parejas en la queambos miembros son seropositivos. Las variables de riesgo/protectoras identificadas se asociaron con las dimensiones:informativas, motivacionales, de habilidades conductuales,sociodemográficas, culturales, de salud, sociofamiliares ypolíticas. Asimismo, se evidenciaron consecuencias sociales,económicas y de salud.(AU)
The objective of this work is to characterize partner violencein people with seropositivity (prevalence, types of violentactions, risk/protective variables, and consequences), for whicha systematic review was carried out through the PRISMAprotocol. The search for articles was carried out in Scopus,Web of Science, Eric, Scielo and Pubmed published up to 2021. 113 articles were found, of which 22 met the eligibilitycriteria. The results indicate that intimate partner violencein seropositive people, in addition to manifesting itself ina physical, psychological, patrimonial, sexual, and verbalway, can be presented using HIV to exercise violence. Theprevalence varies depending on the geographical contextin a range from 19.6% to 43.1%, which is higher in themigrant population and in men who have sex with men(MSM), seropositive women, non-heterosexual couples andin couples in which both members are seropositive. Therisk/protective variables identified were associated with thedimensions: informational, motivational, behavioral skills,sociodemographic, cultural, health, socio-family and political.Likewise, social, economic and health consequences wereevidenced.(AU)
O objetivo deste trabalho é caracterizar a violência conjugalem pessoas com soropositividade (prevalência, tipos de açõesviolentas, variáveis de risco/proteção e consequências), paraa qual foi realizada uma revisão sistemática por meio doprotocolo PRISMA. A busca de artigos foi realizada no Scopus,Web of Science, Eric, Scielo e Pubmed publicados até 2021.Foram encontrados 113 artigos, dos quais 22 atenderamaos critérios de elegibilidade. Os resultados indicam que aviolência por parceiro íntimo em pessoas soropositivas, alémde se manifestar de forma física, psicológica, patrimonial,sexual e verbal, pode se apresentar por meio do uso do HIVpara exercer a violência. A prevalência varia de acordo como contexto geográfico em uma faixa de 19,6% a 43,1%; queé maior na população migrante e em homens que fazemsexo com homens (HSH), mulheres soropositivas, casais nãoheterossexuais e em casais em que ambos os membros sãosoropositivos. As variáveis de risco/proteção identificadasforam associadas às dimensões: informacional, motivacional,habilidades comportamentais, sociodemográficas, culturais,de saúde, sociofamiliares e políticas. Da mesma forma, foramevidenciadas consequências sociais, econômicas e de saúde.(AU)
Asunto(s)
Humanos , Masculino , Femenino , Violencia Doméstica , Violencia de Pareja , Seropositividad para VIH , Violencia de GéneroRESUMEN
When attacked, many decapod crustaceans perform tailflips, which are triggered by a neural circuit that includes lateral giant interneurons, medial giant interneurons, and fast flexor motor giant neurons (MoGs). Slipper lobsters (Scyllaridae) lack these giant neurons, and it has been hypothesized that behavioral (e.g., digging) and morphological (e.g., flattening and armor) specializations in this group caused the loss of escape-related giant neurons. To test this hypothesis, we examined a species of spiny lobster, Panulirus argus. Spiny lobsters belong to the sister taxon of the scyllarids, but they have a more crayfish-like morphology than scyllarids and were predicted to have escape-related giant neurons. Ventral nerve cords of P. argus were examined using paraffin-embedded sections and cobalt backfills. We found no escape-related giant neurons and no large axon profiles in the dorsal region of the nerve cord of P. argus. Cobalt backfills showed one fewer fast flexor motor neuron than in species with MoGs and none of the fast flexor motor neurons show any of the anatomical specializations of MoGs. This suggests that all palinuran species lack this giant escape circuit, and that the loss of rapid escape behavior preceded, and may have driven, alternative predator avoidance and anti-predator strategies in palinurans.
Asunto(s)
Ganglios de Invertebrados/fisiología , Neuronas/fisiología , Palinuridae/anatomía & histología , Palinuridae/fisiología , Animales , Astacoidea/anatomía & histología , Astacoidea/fisiología , Axones/fisiología , Reacción de Fuga/fisiología , Ganglios de Invertebrados/anatomía & histología , Conducta PredatoriaRESUMEN
Background: Hydatid disease or cystic echinococcosis, caused by the parasite Echinococcus granulosus, has a worldwide distribution, affecting people of working age and can cause high levels of morbidity and even death. Aim: To estimate the economic impact at the human and animal level caused by the disease in Chile. Material and Methods: We analyzed information about the disease obtained from reports and publications emanated from the Chilean Ministry of Health, United Nations Food and Agriculture Organization, the U.S. National Institute of Statistics and the National Agricultural Service. Animal derived costs were estimated evaluating the expenses for pharmacological treatment of infected dogs and animal production losses derived from confiscations and reductions in meat production. Results: The total number of patients who underwent surgery to remove a hydatid cyst in Chile during 2012, was estimated as 767 individuals. The annual costs derived only from surgical treatment, were estimated in USD 2.46 million. Summing the costs of sick leaves and loss of productivity, the costs at the human level ascended to USD 3.13 million. Considering human and animal costs, the annual economic burden of the disease was estimated in USD 14.35 million. Conclusions: The Analysis of the regional distribution of human and animal hydatidosis, suggests a significant environmental contamination with parasite eggs in high incidence regions such as Aysén, Araucanía, BioBío and Coquimbo. The efficiency of control programs for the disease would be greatly improved if the causes for these regional contaminations are elucidated.
Asunto(s)
Animales , Perros , Humanos , Costo de Enfermedad , Equinococosis/economía , Costos de la Atención en Salud , Crianza de Animales Domésticos/economía , Chile/epidemiología , Enfermedades de los Perros/tratamiento farmacológico , Enfermedades de los Perros/economía , Enfermedades de los Perros/epidemiología , Equinococosis/epidemiología , Equinococosis/terapia , Equinococosis/veterinaria , Incidencia , Ausencia por Enfermedad/economíaRESUMEN
En México, como en otros países, el envejecimiento demográfico ha sido un tema abordado en diversas investigaciones y con distintos énfasis disciplinares. Las implicaciones que traerá consigo contar con un gran número de personas con 65 años o más es un tema que ha adquirido gran relevancia en la actualidad. No obstante, hay que indicar que el envejecimiento no se vive igual en todas las latitudes, mientras que en muchos países europeos se cuentan con sistemas de salud y seguridad social para la población envejecida, en algunos países latinoamericanos el derecho a servicios de salud y la obtención deingresos una vez que se ha concluido la vida laboral siguen siendo temas de preocupación para los gobiernos. México forma parte de los países que enfrentan dificultades para atender a la población envejecida actual y la futura. Frente a este panorama las redes informales de apoyo, como la familia, son de gran importancia para aliviar las dificultades económicas y de salud a las que se enfrenta la población envejecida. Es en este contexto que el arreglo familiar en el que se encuentran las personas con 65 años o más podría responder a una estrategia para aliviar situaciones socioeconómicas precarias. En particular en este trabajo se tiene por objetivo ofrecer un panorama de la cobertura social de la población adulta mayor en México y su vinculación con el tipo de arreglo familiar en el que residen.
In Mexico, as in other countries, demographic ageing has been an issue studied from diverse areas and with different disciplinary emphasis. Implications which will bring with it having a large number of people 65 years or older is a matter which has gained great relevance nowadays. However, it is important to indicate that aging is not living equal in all latitudes, while many European countries have strong health systems and social security for older persons, in some Latin American countries the right to health care and income once work has been completed remain matters of concern to Governments. Mexico is part of the countries that face difficulties addressing the present and the future ageing population. Faced with this panorama, informal networks of support, such as the family, are important to relieve economic hardship and health facing the older people. It isin this context that family arrangement in which are persons age 65 or older could respond to a "strategy" relieving precarious socio-economic situations. In particular the aim of this work is to provide an overview of the social coverage of the adult population in Mexico and linking it to the type of family arrangement in which they reside.
Asunto(s)
Humanos , Anciano , Anciano , Relaciones Familiares , Política PúblicaRESUMEN
En México, como en otros países, el envejecimiento demográfico ha sido un tema abordado en diversas investigaciones y con distintos énfasis disciplinares. Las implicaciones que traerá consigo contar con un gran número de personas con 65 años o más es un tema que ha adquirido gran relevancia en la actualidad. No obstante, hay que indicar que el envejecimiento no se vive igual en todas las latitudes, mientras que en muchos países europeos se cuentan con sistemas de salud y seguridad social para la población envejecida, en algunos países latinoamericanos el derecho a servicios de salud y la obtención deingresos una vez que se ha concluido la vida laboral siguen siendo temas de preocupación para los gobiernos. México forma parte de los países que enfrentan dificultades para atender a la población envejecida actual y la futura. Frente a este panorama las redes informales de apoyo, como la familia, son de gran importancia para aliviar las dificultades económicas y de salud a las que se enfrenta la población envejecida. Es en este contexto que el arreglo familiar en el que se encuentran las personas con 65 años o más podría responder a una estrategia para aliviar situaciones socioeconómicas precarias. En particular en este trabajo se tiene por objetivo ofrecer un panorama de la cobertura social de la población adulta mayor en México y su vinculación con el tipo de arreglo familiar en el que residen.(AU)
In Mexico, as in other countries, demographic ageing has been an issue studied from diverse areas and with different disciplinary emphasis. Implications which will bring with it having a large number of people 65 years or older is a matter which has gained great relevance nowadays. However, it is important to indicate that aging is not living equal in all latitudes, while many European countries have strong health systems and social security for older persons, in some Latin American countries the right to health care and income once work has been completed remain matters of concern to Governments. Mexico is part of the countries that face difficulties addressing the present and the future ageing population. Faced with this panorama, informal networks of support, such as the family, are important to relieve economic hardship and health facing the older people. It isin this context that family arrangement in which are persons age 65 or older could respond to a "strategy" relieving precarious socio-economic situations. In particular the aim of this work is to provide an overview of the social coverage of the adult population in Mexico and linking it to the type of family arrangement in which they reside.(AU)
Asunto(s)
Humanos , Anciano , Anciano , Relaciones Familiares , Política PúblicaRESUMEN
Se presenta el caso de una mujer de 19 años de edad, víctima de mordedura de araña 72 horas previas a su ingreso hospitalario, cuando se encontraba en una excursión en la selva ecuatoriana. Horas más tarde presentó parestesias de miembros inferiores, vesículas y fiebre. A las 24 horas de la mordedura acudió a una cUnica particular en la ciudad de Quito, donde presentó cuatro convulsiones tónico-c1ónicas generalizadas. Recibió corticoide IV, analgesia, vitamina K. Posteriormente transferida al Hospital Vozandes Quito, donde se evidencia episodio de contractura muscular de dorso y región cervical posterior, sin pérdida de la conciencia, pero con dolor importante. Evolución hospitalaria satisfactoria.