Improved algorithm with adaptive regularization for tomographic reconstruction of gas distributions using DOAS measurements.

Differential optical absorption spectroscopy (DOAS) is notably well suited for the retrieval of UV-absorbing trace gases present in the atmosphere. We combine multi-axis DOAS observations to perform a tomographic reconstruction of the distribution of gases emitted from different sources. We use a new algorithm based on a regularized minimization approach embedding key physical aspects of the solution to constrain the inversion. In this work, we take into account that the spatial sampling of the plume being scanned by the instruments is not homogeneous. Therefore, we introduce an adaptive approach with a locally tuned regularization weight according to the uncertainty levels introduced by the sampling scheme. We tested our approach on reconstructions of simulated gas distributions and different configurations applicable to multi-axis DOAS. Finally, our approach is applied to experimental data for the retrieval of the distribution of ${\rm NO}_2$NO2 within a plume cross section emitted from a group of stacks.

Components of Genetic Variation Associated with Second and Third Chromosome Gene Arrangements in Drosophila melanogaster.

The effects of naturally occuring combinations of second and third chromosome gene arrangements of Drosophila melanogaster on two quantitative traits were partitioned into parameters of additive, dominance and interaction components of genetic variation. Development time and preadult survival of the gene arrangement genotypes were measured under four experimental conditions. Gene arrangement effects, when significant, were predominantly additive under all conditions. Experimental conditions, however, did influence gene arrangement effects. A second chromosome effect on development time was detected when amount of food or temperature was reduced, but not under optimal conditions. A third chromosome additive effect on development was observed under all experimental conditions. A consistent interaction effect between second and third chromosome gene arrangements was detected only at low temperature. Gene arrangement effects on survival were not as consistent as for development time, but also depended on experimental conditions.

Esterase B1 activity variation within and among insecticide resistant, susceptible and heterozygous strains of Culex quinquefasciatus (Diptera: Culicidae).

Amplification of the esterase B1 gene of Culex quinquefasciatus Say results in high titers of an esterase enzyme that confers resistance to organophosphate insecticides. Esterase activity of individuals was measured in samples from an organophosphate resistant strain (Tem-R), a susceptible strain (S-Lb), and their reciprocal F1 progeny. Within-strain variation, as measured by coefficients of variation, was fairly consistent between sexes within strains and among strains (average, 12%). On average, individuals from the Tem-R strain had about 120 times the esterase activity of individuals from the S-Lab strain. The mean esterase activities of the F1 strains were significantly higher than the average of the Tem-R and S-Lab strain mean esterase activities, suggesting enhanced expression of the amplified esterase B1 genes in F1 individuals. Reciprocal F1 strains did not differ significantly in esterase activity or resistance, indicating that maternal effects do not influence either of these measures in these strains. The levels of esterase activity of the strains are discussed in relation to their resistance.

Baseline susceptibility to bacterial insecticides in populations of Culex pipiens complex (Diptera: Culicidae) from California and from the Mediterranean Island of Cyprus.

Bacterial insecticides play an increasingly important role in mosquito control. To establish guidelines for detecting resistance at an early stage, information on natural variation in susceptibility of insect populations to these insecticides is needed. Between 1990 and 1993, the susceptibility of Culex pipiens L. complex to Bacillus thuringiensis subsp. israelensis de Barjac and/or Bacillus sphaericus Neide was determined in 31 collections from California. These collections were undertaken before the widespread use of B. thuringiensis subsp. israelensis and before the registration of B. sphaericus in California. Seven collections from the Mediterranean island of Cyprus, where no microbial insecticides have been used, also were tested. The 1990-1991 California collections exhibited limited variation in susceptibility to B. thuringiensis subsp. israelensis. LC50 and LC95 values spanned about a three-fold and four-fold range, respectively. The 1993 Cyprus collections exhibited both higher mean LC values, and greater variability in those values, than the California collections. The LC50s for the Cyprus collections varied over a 10-fold range, whereas the LC50s varied over a 12.5-fold range. Variation in susceptibility to B. sphaericus among the 1991 California collections was about five-fold at the LC50 and LC95. No significant geographic variation in susceptibility to B. thuringiensis subsp. israelensis was observed among regions within California. Although variation in susceptibility was limited among California collections, the greater variability observed among the Cyprus collections and between the Cyprus and California collections illustrates the importance of establishing regional baselines to monitor accurately for changes in susceptibility.

Quantitative genetic variation of esterase activity associated with a gene amplification in Culex quinquefasciatus.

Amplification of the esterase B1 gene is responsible for insecticide resistance in the mosquito Culex quinquefasciatus. We used a mating scheme to isolate chromosomes carrying amplified esterase genes from a long-selected laboratory strain (Tem-R) to determine whether observed variation in esterase activity had a genetic basis. The amplified esterase genes segregated as a block and a possible newly arisen esterase B1 copy-number variant was found among the progeny of females which carried amplified B1 genes on only one homologue. A quantitative genetic analysis found significant genetic variation of esterase activity among families which carried different amplification-bearing chromosomes from the Tem-R strain. Esterase B1 copy-number variation among these Tem-R chromosomes is the most likely basis for the observed genetic variation in esterase activity.

Visualization of two-dimensional phase gradients by subtraction of a reference periodic pattern.

We describe a simple method for visualization of phase objects. The phase object is placed between a printed two-dimensional periodic pattern and a CCD camera. The ray deflection that is due to the phase object distorts the image of the pattern. This image is subtracted from a reference image and then, by squaring and low-pass filtering, a measurement of the two-dimensional refractive-index changes is obtained. Because the optical system does not require special alignment or illumination, the method presented has potential application for detection of gas leaks in industrial environments.

Optical-fiber vibration sensor using step interferometry.

An all-fiber vibration sensor based on step interferometry is described. The sensor consists of a modified Michelson interferometer in which the ends of the reference and signal arms are assembled and fixed together to produce regular distributed interference fringes. Five photodetectors with relative phase shifts of π/2 placed on the fringes acquire five intensity patterns simultaneously. One reconstructs the vibration amplitude by using the well-known five-step algorithm. A vibration sensor with these characteristics was constructed, and its performance was investigated.

Current sensor using heterodyne detection.

Based on the Faraday effect for measuring ac current, we describe a fiber-optic sensor that uses laser-diode intensity modulation to perform heterodyne signal detection. The sensor output at the carrier frequency is used as a reference signal to normalize the results. The sensing element consists of a few coils low-birefringence fibers between polarizers. We built the current sensor described above and tested its performance--sensitivity and noise--as functions of the angle between polarizers.

Breeding structure of a colonising species: Aedes albopictus (Skuse) in the United States.

Allelic and genotypic frequencies were monitored at seven enzymatic loci in newly established Aedes albopictus (Skuse) populations in the United States. Populations were sampled within the cities of New Orleans, Louisiana, Houston, Texas, three counties surrounding Houston, Memphis, Tennessee, Jacksonville, Florida, and Evansville and Indianapolis, Indiana. Unique alleles and relatively high levels of heterozygosity were detected in New Orleans, Houston, surrounding counties and Indianapolis suggesting relatively large and independent introductions in these cities. No unique alleles and low heterozygosities were detected in Memphis, Evansville and Jacksonville suggesting that a population bottleneck may have accompanied the founding of these populations. The bottleneck may have resulted from a small number of founding individuals or may have been generated through repeated control efforts. Genetic distance estimates indicated that Houston, New Orleans and Indianapolis were genetically similar. Evansville and Memphis were also found to be similar. Significant differentiation of allele frequencies existed among and within cities. Variance in allele frequencies among all samples was partitioned into the variance among cities and among locations within cities. Most of the variance was attributable to local differentiation. The most parsimonious explanation of this result is that much genetic drift accompanied the establishment of local populations in cities and that there has been little subsequent gene flow. Analysis of genotypic frequencies detected a slight but consistent excess of homozygotes suggesting inbreeding.

Conversion of bright nondiffracting beams into dark nondiffracting beams by use of the topological properties of polarized light.

We present a method for the generation of an axial phase dislocation on a wave front, which is induced by topological properties of polarized light. This effect is shown to be useful for conversion of bright nondiffracting beams into dark nondiffracting beams. Experiments showing the generation of dark nondiffracting beams have been performed.

Alternating current sensor with second-harmonic detection.

We describe a novel optical sensor based on the Faraday effect for measuring ac. The sensing element is a piece of material of high Verdet constant placed between polarizers. When the angle between the polarizers differs by 45 degrees, a signal of twice the frequency of the ac is generated--in addition to the usual signal with the same frequency as the current. The quotient of these two signals allows a normalized output to be obtained that is proportional to the amplitude of the current. Experimental results demonstrating the practicality of the procedure are presented.

Bi-dimensional fluid inclinometer.

A gravity referenced optical inclinometer which uses a transparent fluid as angle-sensor element is described. Its principle of operation is based on light refraction at a free surface of the fluid. Measurements were carried out in an optical inclinometer built by the authors, and a resolution of 0.007° was obtained. The inclinometer permits real-time angle determinations along two perpendicular axis.

Robust one-beam interferometer with phase-delay control.

A robust one-beam interferometer with external phase-delay control is described. The device resembles a Mach-Zehnder interferometer in which the two arms are together in one collimated beam. However, the proposed device is not an amplitude-division interferometer but a wave-front division one. The phase-delay control occurs at the interferometer output with the help of two polarizing beam splitters, a quarter-wave plate, a Faraday rotator, and a polarizer. An additional phase delay is introduced by application of an electrical current to the Faraday rotator or by rotation of the polarizer (the latter is of topological origin), which permits the use of techniques of phase-stepping interferometry.

Precision synchronous polarimeter with linear response for the measurement of small rotation angles.

A synchronous polarimeter was set up for the measurement of small rotation angles of the polarization plane of light. The polarimeter is based on a polarizer-Faraday modulator-analyzer structure with a synchronous detection scheme, which produces a linear system response. The theoretical background is studied, and the system performance is investigated experimentally. We achieved an accuracy of the order of 10(-4) deg, or 5 mg/dl of glucose in a 1-cm light path.