Quality of different obturation techniques to fill perforating internal root resorption: a micro-computed tomographic study.

BACKGROUND: This study aimed to assess the quality of various obturation techniques to fill perforation caused by internal root resorption using Micro-computed Tomography. METHODS: Cone-beam computed tomography images of a maxillary central incisor tooth with perforating internal resorptive defect were used to create a 3D printed model of the affected tooth. The replicas were divided into four groups based on the obturation technique used. The techniques included Group 1: a polydimethylsiloxane-based sealer (GuttaFlow-2) with gutta-percha. Group 2: same as Group 1 except for using a pre-mixed Bioceramic-based sealer (NeoSEALER Flo). Group 3: the defect was filled entirely using the NeoSealer Flo Bioceramic-based sealer. Group 4: the samples were obturated using the warm vertical compaction technique with a resin-based sealer (ADSeal). The resin models were then scanned a micro-computed scanner to evaluate the percentage of voids in each group. RESULTS: The results showed that NeoSEALER Flo groups had significantly the highest volume of voids while GuttaFlow-2 and warm vertical compaction groups had the lowest void volume. CONCLUSIONS: GuttaFlow-2 and warm vertical compaction techniques performed best in filling the internal resorptive defect.

Shape optimization of a 2-unit cantilevered posterior resin-bonded fixed dental prosthesis.

STATEMENT OF PROBLEM: The cantilevered resin-bonded fixed dental prosthesis (RBFDP) is a feasible and minimally invasive treatment option to restore a single missing tooth, especially when the missing tooth space is small (<7 mm) and cost-effectiveness is essential. However, its long-term survival needs to be improved by increasing its structural strength and interfacial adhesion. PURPOSE: The purpose of this study was to improve the interfacial bonding and to enhance the structural strength of a 2-unit inlay-retained cantilevered RBFDP with a 2-step numerical shape optimization. MATERIAL AND METHODS: A finite element model of a mandibular first molar with a second premolar pontic was constructed. A load of 200 N simulating the average occlusal force was applied on the mesial fossa of the pontic. In the first step, an in-house user-defined material subroutine was used to generate the cavity preparation. The subroutine iteratively changed the tooth tissues next to the pontic to composite resin according to the local stresses until convergence was achieved. In the second step, the subroutine was used to optimize the placement of fibers in the pontic by placing fibers in high-stress regions. To assess the debonding resistance and load capacity of the optimized and conventional designs, further analyses were conducted to compare their stresses at the tooth-restoration interface and those within the restoration. RESULTS: Shape optimization resulted in a shovel-shaped cavity preparation and a pontic with fibers placed near the occlusal surface of the connector region. With the optimized cavity preparation only, the maximum principal stress within the restoration and the tooth structure was reduced from 639.4 MPa to 525.4 MPa and from 381.7 MPa to 352.8 MPa, respectively. With the embedded fibers, the shovel-shaped cavity preparation reduced the maximum interfacial tensile stress by approximately 70% (conventional: 189.6 MPa versus optimized: 57.0 MPa) and the peak maximum principal stress of the veneering composite resin by 45% (conventional: 638.8 MPa versus optimized: 356.5 MPa). The peak maximum principal stress was also reduced for the remaining tooth structure by approximately 30% (conventional: 372.2 MPa versus optimized: 253.1 MPa). CONCLUSIONS: Shape optimization determined that a shovel-shaped retainer with fibers placed near the occlusal surface of the connector area can collectively reduce the interfacial and structural stresses of the 2-unit cantilevered fiber-reinforced RBFDP. This may offer a more conservative treatment option for replacing a single missing tooth.

Effects of concentration of sodium hypochlorite as an endodontic irrigant on the mechanical and structural properties of root dentine: A laboratory study.

AIM: The use of high-concentration sodium hypochlorite (NaOCl) as an endodontic irrigant remains controversial because of its potential impact on the fracture strength of endodontically treated teeth. This study evaluated the effects of using different NaOCl concentrations, with 2-min-ethylenediaminetetraacetic acid (EDTA) as the final active irrigant, on the biomechanical and structural properties of root dentine. METHODOLOGY: A new test method, which is more clinically relevant, was utilized to calculate the fracture strength of root dentine. Bovine incisors were used to obtain root dentine discs. The root canals were enlarged to mean diameter of 2.90 mm with a taper of 0.06. The resulting discs were divided into five groups (n = 20) and treated with different concentrations of NaOCl (5.25%, 2.5%, and 1.3%) for 30 min plus 17% EDTA for 2 min. The discs were then loaded to fracture by a steel rod with the same taper through the central hole. The fractured specimens were examined by scanning electron microscopy to evaluate changes in the dimensions of the remaining intertubular dentine and the tubular radius. Micro-hardness was also measured with a Knoop diamond indenter along a radius to determine the depth of dentine eroded by the irrigation. Results were analysed by one-way anova and the Tukey test. The level of significance was set at α = 0.05. RESULTS: The damage by NaOCl increased with its concentration. 5.25% NaOCl greatly reduced the fracture strength of root dentine from 172.10 ± 30.13 MPa to 114.58 ± 26.74 MPa. The corresponding reduction in micro-hardness at the root canal wall was 34.1%. The damages reached a depth of up to 400 µm (p < .05). Structural changes involved the degradation of the intratubular wall leading to enlarged dentinal tubules and the loss of intertubular dentine. Changes in the microstructural parameters showed positive linear relationships with the fracture strength. CONCLUSIONS: With the adjunctive use of EDTA, NaOCl caused destruction to the intratubular surface near the root canal and, consequently, reduced the root dentine's mechanical strength. The higher the concentration of NaOCl, the greater the effect. Therefore, endodontists should avoid using overly high concentration of NaOCl for irrigation to prevent potential root fracture in endodontically treated teeth.

In vitro efficacy of a non-instrumentation technique to remove intracanal multispecies biofilm.

AIM: The aim of this study was to assess the efficacy of a non-instrumentation technique to disinfect root canals infected by a human dental plaque-derived multispecies biofilm. METHODOLOGY: Twenty-two mandibular incisors were accessed, autoclaved and inoculated with dental plaque. The Center for Disease Control biofilm reactor was used to promote contamination of the root canal space. In the conventional technique (control), the specimens were instrumented until size 35/04 and irrigated with 6% NaOCl. In the non-instrumentation technique, a glide path was established using K-files size 10-20 and specimens were immediately cleaned with the GentleWave System. Samples were obtained for culture and 16S rRNA gene sequencing. Differences in abundances of genera were evaluated using Kruskal-Wallis test, and differences in alpha diversity were compared using anova. Alpha and beta diversity indices were calculated using mothur. The Shannon and Chao1 indices were used to measure alpha diversity. The Bray-Curtis dissimilarity was used to measure beta diversity. Differences in community composition were evaluated using analysis of similarity with Bonferroni correction for multiple comparisons. RESULTS: The total numbers of reads in biological samples ranged from 126 to 45 286. Significantly fewer reads were obtained from samples following cleaning by either method (p < .0001), and significantly fewer reads were obtained in post-cleaning samples following conventional versus non-instrumentation cleaning regiment (p = .002). Communities in pre-treatment samples were similar in both groups; however, significantly greater relative abundances of Streptococcus, Veillonella and Campylobacter were observed following cleaning using non-instrumentation technique (Kruskal-Wallis p = .009, .033, and .001, respectively). Whilst no significant differences were observed in Shannon alpha diversity, the Chao1 index was significantly lower in post-cleaning samples. CONCLUSIONS: Significant shifts in composition were observed following cleaning by using both regimens, but the impact of this change was greater following a conventional cleaning technique.

Validation of a shape-optimized 2-unit cantilevered inlay-retained fiber-reinforced resin-bonded dental prosthesis.

STATEMENT OF PROBLEM: Current designs of fiber-reinforced composite (FRC) resin-bonded fixed dental prostheses (RBFDPs) have a limited lifespan, failing mainly through veneer-fiber delamination, debonding, and fracture. PURPOSE: The purpose of this in vitro study was to validate a new inlay-retained 2-unit cantilevered RBFDP with an optimized cavity and fiber layout proposed in a previous study by using simulated occlusal loading. MATERIAL AND METHODS: Two groups of specimens (n=20), 1 with and 1 without glass fibers, were used to test the influence of the cavity design and that of the fiber layout on their load capacity, respectively. The specimens without fibers were directly cut from a resin-ceramic block by using a computer-aided manufacturing system, while those with fibers were manually fabricated with unidirectional glass fibers and composite resin in a silicone mold. The specimens with and without fibers were attached to abutments made of the same resin-ceramic with a cyanoacrylate-based adhesive and a resin-based dental cement, respectively. An increasing compressive load was applied on the mesial fossa of the premolar pontic until failure. Cracking in the specimens during loading was monitored with a 2-channel acoustic emission (AE) system. RESULTS: All the specimens without fiber reinforcement debonded from the abutments. Those using the optimized shovel-shaped cavity design had a mean ±standard deviation failure load (50.0 ±17.3 N) that was 193% higher than that of those with the conventional step-box design (17.1 ±6.2 N; P<.001). No significant difference was found between the groups for the mean number of AE events per specimen (step-box: 49 ±34 versus shovel-shaped: 63 ±34; P=.427), the mean amplitude of each event (58.4 ±1.3 dB versus 59.5 ±2.4 dB; P=.299), or the mean time to failure (283.2 ±122.3 seconds versus 297.5 ±66.7 seconds; P=.798). Between the groups of specimens with reinforcing fibers, the mean failure load of the conventional design was approximately half that of the optimized one. Again, no significant difference was found for the mean number of AE events per specimen (conventional: 28 ±18 versus optimized: 52 ±53; P=.248) or the mean amplitude for each AE event (64.9 ±4.2 dB versus 61.7 ±5.2 dB; P=.187). The connectors of 8 fiber-reinforced specimens with the conventional design fractured; the other 2 debonded from the abutments. Half of the shape-optimized fiber-reinforced specimens had fractured abutments, but the cantilevers remained intact, 4 specimens fractured at the connector, and only 1 debonded from its abutment. CONCLUSIONS: The shape-optimized 2-unit cantilevered FRC RBFDP had a higher load capacity than the conventional design.

Effects of tightening torque on screw stress and formation of implant-abutment microgaps: A finite element analysis.

STATEMENT OF PROBLEM: The mechanical behavior of the conical connection implant with different torque levels requires evaluation. PURPOSE: The purpose of this finite element analysis study was to investigate the impact of abutment screw torque on the formation of microgaps at the implant-to-abutment interface of a conical connection under oblique loading. This is important because it is thought that bacteria can invade the internal implant space through the abutment-implant microgaps, causing peri-implantitis. MATERIAL AND METHODS: Three-dimensional finite element analyses of the conical implant-abutment connection were performed by using screw torques of 20 Ncm and 30 Ncm. Oblique loads from 10 N to 280 N were applied to the prosthesis placed on the implant. The maximum von Mises stress in the abutment screw, the microgap formation process, and the critical load for bridging the internal implant space were evaluated. RESULTS: The stresses in the abutment screw under oblique loading had limited sensitivity to the screw torque. However, the residual stress in the screw with a 30-Ncm torque was 35% higher than that with a 20-Ncm torque in the absence of an external load. The area in contact at the implant-to-abutment interface decreased with increasing load for both torque values. The critical load for bridging the internal implant space was 160 N for a screw torque of 20 Ncm and 220 N for a screw torque of 30 Ncm. The maximum gap size was approximately 470 µm with all the loads. CONCLUSIONS: Increasing the screw torque can reduce the formation of microgaps at the implant-to-abutment interface. However, this will result in higher mean stress in the abutment screw, which may reduce its fatigue life and consequently that of the prosthesis. Further research is needed to evaluate the relationship between the abutment screw torque and microleakage in implant-supported restorations.

Technical note: Artificial Resynthesis Technology for the experimental formation of dental microwear textures.

Dental microwear formation on the posterior dentition is largely attributed to an organism's diet. However, some have suggested that dietary and environmental abrasives contribute more to the formation process than food, calling into question the applicability of dental microwear to the reconstruction of diet in the fossil record. Creating microwear under controlled conditions would benefit this debate, but requires accurately replicating the oral environment. This study tests the applicability of Artificial Resynthesis Technology (ART 5) to create microwear textures while mitigating the challenges of past research. ART 5 is a simulator that replicates the chewing cycle, responds to changes in food texture, and simulates the actions of the oral cavity. Surgically extracted, occluding pairs of third molars (n = 2 pairs) were used in two chewing experiments: one with dried beef and another with sand added to the dried beef. High-resolution molds were taken at 0, 50, 100, 2500, and 5000 simulated chewing cycles, which equates to approximately 1 week of chewing. Preliminary results show that ART 5 produces microwear textures. Meat alone may produce enamel prism rod exposure at 5000 cycles, although attrition cannot be ruled out. Meat with sand accelerates the wear formation process, with enamel prism rods quickly obliterated and "pit-and-scratch" microwear forming at approximately 2500 cycles. Future work with ART 5 will incorporate a more thorough experimental protocol with improved controls, pH of the simulated oral environment, and grit measurements; however, these results indicate the potential of ART 5 in untangling the complex variables of dental microwear formation.

ECplot: an online tool for making standardized plots from large datasets for bioinformatics publications.

MOTIVATION AND RESULTS: We have implemented ECplot, an online tool for plotting charts from large datasets. This tool supports a variety of chart types commonly used in bioinformatics publications. In our benchmarking, it was able to create a Box-and-Whisker plot with about 67 000 data points and 8 MB total file size within several seconds. The design of the tool makes common formatting operations easy to perform. It also allows more complex operations to be achieved by advanced XML (Extensible Markup Language) and programming options. Data and formatting styles are stored in separate files, such that style templates can be made and applied to new datasets. The text-based file formats based on XML facilitate efficient manipulation of formatting styles for a large number of data series. These file formats also provide a means to reproduce published figures from raw data, which complement parallel efforts in making the data and software involved in published analysis results accessible. We demonstrate this idea by using ECplot to replicate some complex figures from a previous publication. AVAILABILITY AND IMPLEMENTATION: ECplot and its source code (under MIT license) are available at https://yiplab.cse.cuhk.edu.hk/ecplot/. CONTACT: kevinyip@cse.cuhk.edu.hk.

An anatomical investigation of the mandibular second molar using micro-computed tomography.

PURPOSE: An understanding of root anatomy is an important foundation for providing successful endodontic treatment. The aim of this study was to use micro-computed tomography (micro-CT) to investigate the root anatomy of the mandibular second molar. METHODS: Eighteen mandibular second molars were scanned using micro-CT. Images were reconstructed, and measurements and observations were recorded regarding pulpal floor anatomy, canal configuration, root wall thickness along the root, presence of calcifications in the pulp chamber and in canals, and apical anatomy. RESULTS/CONCLUSIONS: The most frequently found mesial root canal configuration was Vertucci Type 7 (1-2-1-2), which was seen in 33.3% of samples. Distal canals were most frequently Vertucci Type 1 (one canal), with 61.1% of samples showing this configuration. 11.1% of samples had two canals, 44.4% of samples had three canals, 33.3% of samples had four canals, and 11.1% of samples had five canals at some point along the length of the roots. Average root wall thickness between the mesiobuccal canal and the furcation was 1.23 mm. Mesiolingual canal root wall thickness was on average 1.29 mm, and the distal root furcation wall thickness averaged 1.41 mm. 77.8% of samples had calcifications present in both the pulp chamber and within the canals.

Comparison of the correlation of photoelasticity and digital imaging to characterize the load transfer of implant-supported restorations.

STATEMENT OF PROBLEM: Whether splinting or not splinting adjacent implants together can optimize the stress/strain transfer to the supporting structures remains controversial. PURPOSE: The purpose of this study was to compare the photoelasticity and digital image correlation (DIC) in analyzing the stresses/strains transferred by an implant-supported prosthesis. MATERIAL AND METHODS: A polymethylmethacrylate model was made with a combination of acrylic resin replicas of a mandibular first premolar and second molar and threaded implants replacing the second premolar and first molar. Splinted (G1/G3) and nonsplinted (G2/G4) metal-ceramic screw-retained crowns were loaded with (G1/G2) and without (G3/G4) the presence of the second molar. Vertical static loads were applied to the first molar implant-supported crown (50 N-photoelasticity; 250 N-DIC). The resulting isochromatic fringes in the photoelastic models were photographed, and a single-camera 2-dimensional DIC system recorded the deformation at the surface of the resin models. RESULTS: Residual stresses were present in the photoelastic model after screw fixation of the crowns. The following average photoelastic stress results (MPa) were found around the loaded implant: G1 (20.06), G2 (23.49), G3 (30.86), G4 (37.64). Horizontal strains (εxx, %) between the molars averaged over the length of the loaded implant were found by DIC: G1 (0.08 ± 0.09), G2 (0.13 ± 0.10), G3 (0.13 ± 0.11), G4 (0.16 ± 0.11). Splinted crowns transferred lower stresses to the supporting bone when the second molar was absent. The second molar optimized the stress distribution between the supporting structures even for nonsplinted restorations. CONCLUSIONS: Both methods presented similar results and seemed capable of indicating where issues associated with stress/strain concentrations might arise. However, DIC, while apparently less sensitive than photoelasticity, is not restricted to the use of light-polarizing materials.

A new insight into the mechanism of the tabletability flip phenomenon.

Tabletability is an outcome of interparticulate bonding area (BA) - bonding strength (BS) interplay, influenced by the mechanical properties, size and shape, surface energetics of the constituent particles, and compaction parameters. Typically, a more plastic active pharmaceutical ingredient (API) exhibits a better tabletability than less plastic APIs due to the formation of a larger BA during tablet compression. Thus, solid forms of an API with greater plasticity are traditionally preferred if other critical pharmaceutical properties are comparable. However, the tabletability flip phenomenon (TFP) suggests that a solid form of an API with poorer tabletability may exhibit better tabletability when formulated with plastic excipients. In this study, we propose another possible mechanism of TFP, wherein softer excipient particles conform to the shape of harder API particles during compaction, leading to a larger BA under certain pressures and, hence, better tabletability. In this scenario, the BA-BS interplay is dominated by BA. Accordingly, TFP should tend to occur when API solid forms are formulated with a soft excipient. We tested this hypothesis by visualizing the deformation of particles in a model compressed tablet by nondestructive micro-computed tomography and by optical microscopy when the particles were separated from the tablet. The results confirmed that soft particles wrapped around hard particles at their interfaces, while an approximately flat contact was formed between two adjacent soft particles. In addition to the direct visual evidence, the BA-dominating mechanism was also supported by the observation that TFP occurred in the p-aminobenzoic acid polymorph system only when mixed with a soft excipient.

Effect of a 10-Methacryloyloxydecyl Dihydrogen Phosphate-treated Bioceramic Sealer on the Bond Strength of an Endodontic Fiber Post: Multilayer Composite Disk Models and Ultra-highspeed Imaging Analysis.

PURPOSE: Multiple materials are found in the root canal after fiber-post cementation. The layer of a bioceramic-based (BC) sealer may affect the bond strength (σBS) of the fiber post in the root canal. The purpose of this study was to employ multilayer composite-disk models in diametral compression to investigate whether the bond strength between a fiber post and root dentin can be increased by the application of a primer on the BC sealer. MATERIALS AND METHODS: The multilayers of materials in the root canal required 3D finite-element (FE) stress analyses (FEA) to provide precise σBS values. First, BC sealer was characterized using x-ray powder diffraction (XRD) to determine when the sealer completely set and the types of crystals formed to select which primer to apply to the sealer. We selected a 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP)-based primer to treat the BC sealer before post cementation. Ultra-highspeed (UHS) imaging was utilized to analyze the crack initiation interface. The obtained failure force was used in FE analysis to calculate σBS. RESULTS: UHS imaging validated the fracture interface at the post-dentin junction as FEA simulations predicted. σBS values of the fiber posts placed with various material combinations in the root canal were 21.1 ± 3.4 (only cement/ post), 22.2 ± 3.4 (BC sealer/cement/post) and 28.6 ± 4.3 MPa (10-MDP primer treated BC sealer/cement/post). The 10-MDP-treated BC sealer exhibited the highest σBS (p < 0.05). CONCLUSION: The multilayer composite disk model proved reliable with diametral compression testing. The presence of BC sealer in the root canal does not reduce σBS of the fiber post. Conditioning the BC sealer layer with 10-MDP primer before fiber-post cemen-tation increases σBS.

Post-failure analysis of model resin-composite restorations subjected to different chemomechanical challenges.

OBJECTIVE: The current study aimed to evaluate the effects of different combinations of chemical and mechanical challenges on the failure load, failure mode and composition of the resulting fracture surfaces of resin-composite restorations. METHODS: Three resin composites were used to fill dentin disks (2 mm inner diameter, 5 mm outer diameter, and 2 mm thick) made from bovine incisor roots. The model restorations, half of which were preconditioned with a low-pH buffer (48 h under pH 4.5), were subjected to diametral compression with either a monotonically increasing load (fast fracture) or a cyclic load with a continuously increasing amplitude (accelerated fatigue). The load or number of cycles to failure was noted. SEM was performed on the fracture surfaces to determine the proportions of dentin, adhesive, and resin composite. RESULTS: Both cyclic fatigue and acid preconditioning significantly reduced the failure load and increased the proportion of interfacial failure in almost all the cases, with cyclic fatigue having a more pronounced effect. Cyclic fatigue also increased the amount of adhesive/hybrid layer present on the fracture surfaces, but the effect of acid preconditioning on the composition of the fracture surfaces varied among the resin composites. SIGNIFICANCE: The adhesive or hybrid layer was found to be the least resistant against the chemomechanical challenges among the components forming the model restoration. Increasing such resistance of the tooth-restoration interface, or its ability to combat the bacterial actions that lead to secondary caries following interfacial debonding, can enhance the longevity of resin-composite restorations.

Validation of a double-semicircular notched configuration for mechanical testing of orthodontic thermoplastic aligner materials.

The potential of using specimens with a double-semicircular-notched configuration for performing tensile tests of orthodontic thermoplastic aligner materials was explored. Unnotched and double-semicircular-notched specimens were loaded in tension using a universal testing machine to determine their tensile strength, while finite element analysis (FEA) and digital image correlation (DIC) were used to estimate stress and strain, respectively. The shape did affect the tensile strength, demonstrating the importance of unifying the form of the specimen. During the elastic phase under tension, double-semicircular-notched specimens showed similar behavior to unnotched specimens. However, great variance was observed in the strain patterns of the unnotched specimens, which exhibited greater chance of end-failure, while the strain patterns of the double-semicircular-notched specimens showed uniformity. Considerable agreement between the theoretical (FEA) and practical models (DIC) further confirmed the validity of the double-semicircular-notched models.

A finite element study on the risk of bone loss around posterior short implants in an atrophic mandible.

PURPOSE: This study aimed to evaluate the risk of bone loss around single short molar crown-supporting implants in an atrophic mandible. METHODS: Implants of different lengths (L = 4 or 6 mm) and diameters (Ø = 4.1 or 4.8 mm) were placed in the molar area of an atrophic mandible. Additional control mandible models were simulated for 4.1 mm diameter implants (L = 4, 6, 8, and 10 mm). A vertical masticatory load of 200 N was applied to three or six occlusal contact areas (3ca or 6ca) of the prosthetic crown. The bone strain energy density (SED) of 109.6 µJ/mm3 was assumed to be the pathological threshold for cortical bone. The peri-implant bone resorption risk index (PIBRri) was calculated by dividing the maximum SED of the crestal cortical bone by the SED pathological threshold. RESULTS: Increasing the implant length from 4 to 6 mm, implant diameter from 4.1 to 4.8 mm, and number of contact areas from 3 to 6 reduced the SED and PIBRri values by approximately 20%, 35%, and 40%, respectively, when comparing pairs of models that isolated a specific variable. All models with 6ca had a low bone resorption risk (PIBRri<0.8), while the Ø4.1 short implant with 3ca had a medium (0.8≤PIBRri≤1.0) or high (PIBRri>1.0) resorption risk. CONCLUSIONS: Increasing the diameter or occlusal contact area of a 4 mm short implant in an atrophic mandible resulted in reduced bone resorption risks, similar to or lower than those observed in a regular mandible with standard-length implants.

Effect of artificial aging on the bond durability of fissure sealants.

PURPOSE: To evaluate the effect of artificial aging on the bond durability of fissure sealants in vitro. MATERIALS AND METHODS: Twenty bovine incisors received 4 different sealant treatments and were divided into four groups: 1. Ultraseal XT plus (UX); 2. Enamel Loc (EL); 3. 35% phosphoric acid plus Enamel Loc (PEL); 4. Adper Prompt L-Pop plus Clinpro (PPC). Beam-shaped specimens were prepared and randomly divided into three subgroups. One subgroup underwent the microtensile bond strength (µTBS) test after 24-h storage in 37°C water. The other two subgroups were also subjected to the microtensile bond strength test after 5000 and 10,000 thermal cycles, respectively. Another twelve intact human third molars were sealed using 1 of 3 methods and were divided into 3 groups of 4 each: 1. Ultraseal XT plus; 2. Adper Prompt L-Pop plus Clinpro; and 3. Enamel Loc. Two specimens from each group were immersed in a 50% silver nitrate solution for 24 h, followed by exposure to fluorescent light for 8 h, before being scanned in a micro-CT (microcomputer tomography) machine. The other two were handled in the same way after undergoing 10,000 thermal cycles. The CT images obtained were evaluated. RESULTS: All samples from the EL group were broken during preparation, so no µTBS results were available. After 5000 thermal cycles, the bond strengths of the three other groups (UX, PEL, PPC) decreased significantly (p < 0.05). Longer thermocycling (10,000 cycles) resulted in more decreases in µTBS for group PEL and PPC, while the strength of the UX group remained relatively unchanged. After thermocycling, considerable silver penetration could be seen at the sealant/enamel interface of the EL group in micro-CT images. CONCLUSIONS: The etch-and-rinse procedure for sealant application promotes higher bond strength under artificial aging. Micro-CT, a nondestructive analytical tool, may be used to evaluate the sealant/enamel interface effectively.

Load capacity and fracture modes of instrumented tooth roots under axial compression.

OBJECTIVE: To investigate the influences of root canal instrumentation on the load capacity and fracture modes of tooth roots under axial compression by performing mechanical tests and finite element analysis (FEA). METHODS: Thirty bovine incisor roots were trimmed into cylinders of 5.0 mm diameter. They were randomly divided into two groups, one with root canals instrumented to ∼2.0 mm in diameter, and one without instrumentation. The specimens were fractured under uniaxial compression at a crosshead speed of 0.2 mm/min, and then micro-CT was used to reveal the fracture patterns in three dimensions. FEA was further performed, using the extended finite element method (XFEM), to compare the compression-induced stress distributions and the initiation and propagation of root fractures in both groups. RESULTS: The mean fracture load of the non-instrumented group (2334 ± 436 N) was statistically significantly higher than that of the instrumented group (1857 ± 377 N) (p < 0.01). Three types of root fractures were identified according to the path and length of the cracks: end-face crack, partial-length crack, and full-length crack. As to the fracture modes, the incidence of partial-length root fracture was the highest in both groups (60% for the non-instrumented group and 53.3% for the instrumented group), followed by that of full-length fracture (26.7% and 40%, respectively) and then end-face fracture (13.3% and 6.7%, respectively). The percentage of full-length fracture was slightly higher in the instrumented group. FEA showed that the compression induced higher Tresca stresses but lower maximum principal stresses in the canal walls of the instrumented group. The XFEM simulations predicted that the fracture of both groups initiated from the outer root surface near an end face and propagated axially to the middle third of the root and radially towards the root canal. These numerical results agreed well with our experimental findings. SIGNIFICANCE: Within the limitation of this study, it was found that root canal instrumentation could significantly decrease the load capacity of tooth roots and potentially increase their susceptibility to full-length root fracture under uniaxial compression.

The effect of abutment material stiffness on the mechanical behavior of dental implant assemblies: A 3D finite element study.

PURPOSE: This study aimed to evaluate the stress distribution and microgap formation in implant assemblies with conical abutments made of different materials under an oblique load. MATERIALS AND METHODS: The mechanical behavior of an implant assembly with a titanium abutment was analyzed and compared with that of an assembly with a Y-TZP abutment using finite element analysis (FEA). A torque of 20 Ncm was first applied to the abutment screw, followed by oblique loads of 10 N-280 N applied to the prosthesis placed on the implant. The maximum stress in the abutment screw, the microgap formation process, and the critical load for bridging the internal implant space were evaluated. RESULTS: No significant difference in stress distribution between the two cases was observed, with the stresses being mainly concentrated at the top half of the screw (the predicted maximum von Mises stress was approximately 1200 MPa at 280 N). The area in contact at the implant-to-abutment interface decreased with increasing load for both abutments, with the critical load for bridging the internal implant space being roughly 140 N. The maximum gap size being was approximately 470 µm with either abutment. CONCLUSION: There was no significant difference in the stress distribution or microgap formed between implant assemblies with titanium and Y-TZP abutments having an internal conical connection.

Interfacial integrity of bulk-fill resin composite restorations in deep Class-II cavities.

This study evaluated the interfacial integrity of deep (6 mm) Class-II (OM/OD) restorations placed using different bulk-fill resin composites [Filtek™ Bulk Fill Flowable Restorative (BF) and Filtek™ Bulk Fill Posterior Restorative (BP) (3M ESPE, St. Paul, MN, USA)] of different increment thicknesses (2 or 4 mm). BP was used for capping in all cases, while Filtek™ Z250 Universal Restorative (3M ESPE) was used as the control material. Interfacial debonding was measured during curing through acoustic emission (AE), followed by image analysis using micro-computed tomography and scanning electron microscopy. Microhardness testing was also conducted to assess degree of conversion. Depth of cure was adequate in all restorations. Specimens with 4-mm thick first increment of BF, which had a higher shrinkage strain, produced most AE events and debonding. Thus, bulk filling of deep cavities using bulk-fill resin composites with a high shrinkage strain should be avoided.

Inhibition of Caries around Restoration by Ion-Releasing Restorative Materials: An In Vitro Optical Coherence Tomography and Micro-Computed Tomography Evaluation.

The objective of this study was firstly to assess the demineralization inhibitory effect of ion-releasing restorations in enamel adjacent to restoration using a biofilm caries model and secondly to compare the effect to that in a chemical caries model. Fifty-six bovine incisors were filled with either Surefil one (SuO), Cention N (CN) (both ion-releasing materials), Ketac-Molar (GIC) or Powerfill resin composite (RC). The restored teeth were then randomly divided into 2 groups according to the used caries model (biofilm or chemical caries model). The micro-computed tomography (MicroCt) and optical coherence tomography (OCT) outcome measures used to evaluate demineralization inhibition effects were lesion depth, LD and increase in OCT integrated reflectivity, ΔIR, at five different depths. It was observed that all outcome measures of CN were statistically the same as those of GIC and conversely with those of RC. This was also the case for SuO except for LD, which was statistically the same as RC. When comparing the two caries models, LD of the biofilm model was statistically deeper (p < 0.05) than the chemical model for all four materials. In conclusion, CN and SuO have similar demineralization inhibitory effects as GIC, and the biofilm caries model is more discriminatory in differentiating demineralization inhibitory effects of ion-releasing restorative material.