Mild poikilocapnic hypoxia increases very low frequency haemoglobin oxygenation oscillations in prefrontal cortex.

BACKGROUND: The aim of the study was to investigate the effect of mild cerebral hypoxia on haemoglobin oxygenation (HbO2), cerebrospinal fluid dynamics and cardiovascular physiology. To achieve this goal, four signals were recorded simultaneously: blood pressure, heart rate / electrocardiogram, HbO2 from right hemisphere and changes of subarachnoid space (SAS) width from left hemisphere. Signals were registered from 30 healthy, young participants (2 females and 28 males, body mass index = 24.5 ± 2.3 kg/m2, age 30.8 ± 13.4 years). RESULTS: We analysed the recorded signals using wavelet transform and phase coherence. We demonstrated for the first time that in healthy subjects exposed to mild poikilokapnic hypoxia there were increases in very low frequency HbO2 oscillations (< 0.052 Hz) in prefrontal cortex. Additionally, SAS fluctuation diminished in the whole frequency range which could be explained by brain oedema. CONCLUSIONS: Consequently the study provides insight into mechanisms governing brain response to a mild hypoxic challenge. Our study supports the notion that HbO2 and SAS width monitoring might be beneficial for patients with acute lung disease.

Coupling between Blood Pressure and Subarachnoid Space Width Oscillations during Slow Breathing.

The precise mechanisms connecting the cardiovascular system and the cerebrospinal fluid (CSF) are not well understood in detail. This paper investigates the couplings between the cardiac and respiratory components, as extracted from blood pressure (BP) signals and oscillations of the subarachnoid space width (SAS), collected during slow ventilation and ventilation against inspiration resistance. The experiment was performed on a group of 20 healthy volunteers (12 females and 8 males; BMI=22.1±3.2 kg/m2; age 25.3±7.9 years). We analysed the recorded signals with a wavelet transform. For the first time, a method based on dynamical Bayesian inference was used to detect the effective phase connectivity and the underlying coupling functions between the SAS and BP signals. There are several new findings. Slow breathing with or without resistance increases the strength of the coupling between the respiratory and cardiac components of both measured signals. We also observed increases in the strength of the coupling between the respiratory component of the BP and the cardiac component of the SAS and vice versa. Slow breathing synchronises the SAS oscillations, between the brain hemispheres. It also diminishes the similarity of the coupling between all analysed pairs of oscillators, while inspiratory resistance partially reverses this phenomenon. BP-SAS and SAS-BP interactions may reflect changes in the overall biomechanical characteristics of the brain.

Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: A Bispectral Analysis.

The aim of the study was to assess blood pressure-subarachnoid space (BP-SAS) width coupling properties using time-frequency bispectral analysis based on wavelet transforms during handgrip and cold tests. The experiments were performed on a group of 16 healthy subjects (F/M; 7/9) of the mean age 27.2 ± 6.8 years and body mass index of 23.8 ± 4.1 kg/m2. The sequence of challenges was first handgrip and then cold test. The handgrip challenge consisted of a 2-min strain, indicated by oral communication from the investigator, at 30% of maximum strength. The cold test consisted of 2 min of hand immersion to approximately wrist level in cold water of 4 °C, verified by a digital thermometer. Each test was preceded by 10 min at baseline and was followed by 10-min recovery recordings. BP and SAS were recorded simultaneously. Three 2-min stages of the procedure, baseline, test, and recovery, were analyzed. We found that BP-SAS coupling was present only at cardiac frequency, while at respiratory frequency both oscillators were uncoupled. Handgrip and cold test failed to affect BP-SAS cardiac-respiratory coupling. We showed similar handgrip and cold test cardiac bispectral coupling for individual subjects. Further studies are required to establish whether the observed intersubject variability concerning the BP-SAS coupling at cardiac frequency has any potential clinical predictive value.

Oscillations of Subarachnoid Space Width as a Potential Marker of Cerebrospinal Fluid Pulsatility.

In the cerebrospinal fluid (CSF) circulation, two components can be distinguished: bulk flow (circulation) and pulsatile flow (back and forth motion). CSF pulsatile flow is generated by both cardiac and respiratory cycles. Recent years have seen increased interest in cardiac- and respiratory-driven CSF pulsatility as an important component of cerebral homeostasis. CSF pulsatility is affected by cerebral arterial inflow and jugular outflow and potentially linked to white matter abnormalities in various diseases, such as multiple sclerosis or hypertension. In this review, we discuss the physiological mechanisms associated with CSF pulsation and its clinical significance. Finally, we explain the concept of using the oscillations of subarachnoid space width as a surrogate for CSF pulsatility.

Modelling of subarachnoid space width changes in apnoea resulting as a function of blood flow parameters.

During apnoea, the pial artery is subjected to two opposite physiological processes: vasoconstriction due to elevated blood pressure and vasorelaxation driven by rising pH in the brain parenchyma. We hypothesized that the pial artery response to apnoea may vary, depending on which process dominate. Apnoea experiments were performed in a group of 19 healthy, non-smoking volunteers (9 men and 10 women). The following parameters were obtained for further analysis: blood pressure, the cardiac (from 0.5 to 5.0Hz) and slow (<0.5Hz) components of subarachnoid space width, heart rate, mean cerebral blood flow velocity in the internal carotid artery, pulsatility and resistivity index, internal carotid artery diameter, blood oxygen saturation and end-tidal carbon dioxide. The experiment consisted of three apnoeas, sequentially: 30s, 60s and maximal apnoea. The breath-hold was separated for 5minute rest. The control process is sophisticated, involving internal cross-couplings and cross-dependences. The aim of work was to find a mathematical dependence between data. Unexpectedly, the modelling revealed two different reactions, on the same experimental procedure. As a consequence, there are two subsets of cardiac subarachnoid space width responses to breath-hold in humans. A positive cardiac subarachnoid space width change to apnoea depends on changes in heart rate and cerebral blood flow velocity. A negative cardiac subarachnoid space width change to apnoea is driven by heart rate, mean arterial pressure and pulsatility index changes. The described above two different reactions to experimental breath-hold provides new insights into our understanding of the complex mechanisms governing the adaptation to apnoea in humans. We proposed a mathematical methodology that can be used in further clinical research.

Cerebral blood flow, sympathetic nerve activity and stroke risk in obstructive sleep apnoea. Is there a direct link?

Obstructive sleep apnoea (OSA) is significantly associated with the risk of stroke, and this association is independent of other risk factors, including hypertension, atrial fibrillation and diabetes mellitus. Therefore, additional pathogenic mechanisms may exist, which contribute to the increased risk of stroke. OSA is characterized by prolonged sympathetic overactivity; however the role of the sympathetic nervous system in regulating cerebral circulation remains a matter of controversy. Converging data indicate that brain perfusion is significantly distorted in OSA, with reported decreases in cerebral blood flow as well as intermittent surges in blood pressure and cerebral blood flow velocity. Based on recent research, there is accumulating evidence that sympathetic nerve activity is an important element in brain protection against excessive increases in perfusion pressure during blood pressure surges and flow during rapid eye movement sleep. The aim of this article was to review: (i) the current physiological knowledge related to the role of the sympathetic system in the regulation of cerebral blood flow, (ii) how the influence of the sympathetic system on cerebral vessels is affected by apnoea (increased PaCO(2)) and (iii) the potential significance of the pathological sympathetic system/PaCO(2) interplay in OSA. Sympathetic system seems to be at least partially involved in pathogenesis of distorted haemodynamics and stroke in OSA patients. However, there are still several open questions that need to be addressed before the effective therapeutic strategies can be implemented.

Effects of acute hypercapnia on the amplitude of cerebrovascular pulsation in humans registered with a non-invasive method.

AIM: The aim of the study was to assess non-invasively the effects of acute hypercapnia on the amplitude of cerebrovascular pulsation (CVP) in humans. METHODS: Experiments were carried out in four male volunteers aged 25, 26, 31 and 49. Changes in blood flow through the pial arteries were induced using two interventions: (A) breathing a gas mixture containing 5% CO(2) for 2 minutes and (B) intravenous administration of 1g acetazolamide. The amplitude of CVP and width of subarachnoid space (SAS) were measured non-invasively using near-infrared transillumination/backscattering sounding (NIR-T/BSS), while cerebral blood flow was assessed by single photon emission computed tomography (SPECT) and mean blood flow velocity in the left anterior cerebral artery by transcranial Doppler. RESULTS: Inhalation of a gas mixture containing 5% CO(2) evoked an increase in the amplitude of CVP (202.5% ± SE 10.1), normalized number of counts (22.6% ± SE 3.5%) and mean blood flow velocity in the left cerebral anterior artery (37.6%± SE 11.7%), while resistive index decreased (-8.7% ± SE 2.3%) and the width of SAS decreased (-8.0 ± SE 0.45). Acetazolamide also produced an increase in the amplitude of CVP (23.7% ± SE 5.4%), normalized number of counts (7.9% ± SE 1.1%), and mean blood flow velocity in the left cerebral anterior artery (62.8% ± SE 13.7%), while resistive index decreased (-7.9% ± SE 1.7%), and the width of SAS decreased (-13.4% ± SE 3.4%). CONCLUSION: Acute hypercapnia causes an increase in the amplitude of CVP pulsation in humans. NIR-T/BSS allows for non-invasive bedside monitoring of the amplitude of CVP. NIR-T/BSS is consistent with transcranial Doppler and SPECT.

Flow-induced changes in pial artery compliance registered with a non-invasive method in rabbits.

AIM: This study was performed: 1) to assess the relationship between blood flow velocity in the internal carotid artery (CBF(ICA)) and pial artery pulsation (cc-TQ) and 2) to evaluate flow-induced changes in pial artery compliance. METHODS: Experiments were performed on 10 crossbred male rabbits. Heart rate (HR), blood pressure (BP), left ventricle ejection fraction (LVEF), CBF(ICA), the systolic-diastolic blood volume fraction in the brain circulation (CBF(SDF)) and cc-TQ were recorded after glucagon and acetazolamide administration. cc-TQ was measured with near-infrared transillumination back scattering sounding (NIR-T/BSS), LVEF and CBF(SDF) with gated scintigraphy and BP and CBF(ICA) with electromagnetic pressure and flow transducers, respectively. Doses of drugs were chosen to exert a haemodynamic effect but not change BP or intracranial pressure. RESULTS: Acetazolamide and glucagon evoked significant increases in cc-TQ, CBF(SDF), CBF(ICA), LVEF and HR. The following interdependencies were found: 1) changes after acetazolamide administration compared to baseline: CBF(SDF) vs. LVEF (r=0.73, p<0.05), cc-TQ vs. CBF(SDF) (r=-0.67, p<0.05), cc-TQ vs. LVEF (r=-0.76, p<0.05), 2) changes after glucagon administration compared to baseline: CBF(ICA) vs. BP (r=0.73, p<0.05), CBF(SDF) vs. LVEF (r=0.87, p<0.05), cc-TQ vs. HR (r=0.85, p<0.05), cc-TQ vs. CBF(ICA) (r=-0.74, p<0.05). CONCLUSION: In the absence of systemic BP changes, pial arteries are significantly affected by changes in CBF(ICA). Pial arteries counteract changes in CBF(ICA) and CBF(SDF). The ability of pial arteries to stabilise CBF(ICA) is impaired after acetazolamide administration. Changes in cardiac output directly affect the brain's microcirculation. NIR-T/BSS recordings allow for non-invasive assessment of changes in pial artery compliance.

Effects of the Valsalva maneuver on pial artery pulsation and subarachnoid width in healthy adults.

AIM: The objective was to characterize the effects of Valsalva maneuver (VM) on the amplitude of cerebrovascular pulsation (CVP), and to explore the direct interactions between the cerebral vasculature and the cerebrospinal fluid compartment in VMIII. METHODS: Twenty-nine healthy volunteers between the ages of 25 and 40 (29.3 ± SE 4.0) were studied. Changes in the amplitude of CVP (cc-TQ) and width of subarachnoid space (SAS; sas-TQ) were recorded with NIR-T/BSS sensor. Changes in arterial blood pressure (ABP) and heart rate were measured using Finapres. Cerebral blood flow velocity (CBFV) in the left middle cerebral artery was recorded with transcranial doppler. RESULTS: sas-TQ remained unchanged, while cc-TQ increased in VMI (+40% vs. baseline). In VMIIa, sas-TQ increase (+3.4% vs. baseline) and deep decrease in cc-TQ (-81% vs. baseline) were observed. sas-TQ decrease started in VMIIb (-2.7% vs. baseline), with simultaneous slight increase in cc-TQ (-58% vs. baseline). In VMIII deep sas-TQ decrease (-6.2% vs. baseline) was associated with huge increase in cc-TQ (+110% vs. baseline; r=-0.56, p<0.01). During VMIV sas-TQ increased (-4.8% vs. baseline) while cc-TQ decreased (+38% vs. baseline). The drop of cc-TQ in VMIIa was significantly greater than corresponding changes in CBFV and ABP. Increase in cc-TQ in VMIII preceded CBFV and ABP changes in VMIV. CONCLUSION: The VM evokes significant changes in the amplitude of CVP. Changes in small vessel pulsation precede changes in CBFV. There are direct interactions between cc-TQ and sas-TQ in VMIII. NIR-T/BSS allows for continuous, non-invasive monitoring of the amplitude of CVP and width of the SAS.

Comparison of near infrared spectroscopy (NIRS) and near-infrared transillumination-backscattering sounding (NIR-T/BSS) methods.

The aim of the study was to compare simultaneously recorded a NIR-T/BSS and NIRS signals from healthy volunteers. NIR-T/BSS is a device which give an ability to non-invasively detect and monitor changes in the subarachnoid space width (SAS). Experiments were performed on a group of 30 healthy volunteers (28 males and 2 females, age 30.8 ± 13.4 years, BMI = 24.5 ± 2.3 kg/m2). We analysed recorded signals using analysis methods based on wavelet transform (WT) for the wide frequency range from 0.0095 to 2 Hz. Despite the fact that both devices use a similar radiation source both signals are distinct from each other. We found statistically significant differences for WT amplitude spectra between both signals. Additionally, we showed different relationships of both signals to blood pressure. Collectively, based on the present findings and those of previous studies, we can conclude that the combination of NIR-T/BSS or NIRS signals and time-frequency analysis opens new frontiers in science, and give possibility to understand and diagnosis of various neurodegenerative and ageing related diseases to improve diagnostic procedures and patient prognosis.

Cerebrovascular pulsation and width of subarachnoid space during electroconvulsive therapy.

BACKGROUND: The mechanism of electroconvulsive therapy (ECT) has not been comprehensively explained. A new noninvasive method of monitoring intracranial homeostasis during and after ECT provides additional insight into the mechanism of ECT. The investigations on changes in the width of the subarachnoid space (SAS) and intracranial pulsation amplitude by near infrared-transillumination/backscattering sounding method can throw new light on changes in type and volume induced by ECT. METHOD: In this study, a new method of noninvasive investigation of width of SAS and intracranial pulsation parameters was used. This method has been called near infrared-transillumination/backscattering sounding. The method consists of light beam analysis in near infrared whereby the light beam is emitted by an emitting diode. The light beam passes through head's anatomic layers, is reflected multiple times in SAS, and returns outward-to be recorded by the receiving diode. The novelty of this method is the patented invention that allows skin flow under the probe to be eliminated and thereby "to look" under the skull bone. RESULTS: After ECT, a highly significant increase in pulsation amplitude was observed. The pulsation amplitude rose by a factor of 2.5 times after a mean of 90 seconds after the ECT stimulus. A pause in SAS pulsation-not longer than 26 seconds-directly after administration of electroshocks was observed. After approximately 90 seconds, the amplitude decreased slowly, and this was consistent in all analyzed cases. A decrease in SAS width was also observed.

Impact of slow breathing on the blood pressure and subarachnoid space width oscillations in humans.

The aim of the study was to assess cardiac and respiratory blood pressure (BP) and subarachnoid space (SAS) width oscillations during the resting state for slow and fast breathing and breathing against inspiratory resistance. Experiments were performed on a group of 20 healthy volunteers (8 males and 12 females; age 25.3 ± 7.9 years; BMI = 22.1 ± 3.2 kg/m2). BP and heart rate (HR) were measured using continuous finger-pulse photoplethysmography. SAS signals were recorded using an SAS monitor. Oxyhaemoglobin saturation (SaO2) and end-tidal CO2 (EtCO2) were measured using a medical monitoring system. Procedure 1 consisted of breathing spontaneously and at controlled rates of 6 breaths/minute and 6 breaths/minute with inspiratory resistance for 10 minutes. Procedure 2 consisted of breathing spontaneously and at controlled rates of 6, 12 and 18 breaths/minute for 5 minutes. Wavelet analysis with the Morlet mother wavelet was applied for delineation of BP and SAS signals cardiac and respiratory components. Slow breathing diminishes amplitude of cardiac BP and SAS oscillations. The overall increase in BP and SAS oscillations during slow breathing is driven by the respiratory component. Drop in cardiac component of BP amplitude evoked by slow-breathing may be perceived as a cardiovascular protective mechanism to avoid target organ damage. Further studies are warranted to assess long-term effects of slow breathing.

Regional resting state perfusion variability and delayed cerebrovascular uniform reactivity in subjects with chronic carotid artery stenosis.

The aim of this study was to assess regional perfusion at baseline and regional cerebrovascular resistance (CVR) to delayed acetazolamide challenge in subjects with chronic carotid artery stenosis. Sixteen patients (ten males) aged 70.94±7.71 with carotid artery stenosis ≥ 90% on the ipsilateral side and ≤ 50% on the contralateral side were enrolled into the study. In all patients, two computed tomography perfusion examinations were carried out; the first was performed before acetazolamide administration and the second 60 minutes after injection. The differences between mean values were examined by paired two-sample t-test and alternative nonparametric Wilcoxon's test. Normality assumption was examined using W Shapiro-Wilk test. The lowest resting-state cerebral blood flow (CBF) was observed in white matter (ipsilateral side: 18.4±6.2; contralateral side: 19.3±6.6) and brainstem (ipsilateral side: 27.8±8.5; contralateral side: 29.1±10.8). Grey matter (cerebral cortex) resting state CBF was below the normal value for subjects of this age: frontal lobe - ipsilateral side: 30.4±7.0, contralateral side: 33.7±7.1; parietal lobe - ipsilateral side: 36.4±11.3, contralateral side: 42.7±9.9; temporal lobe - ipsilateral side: 32.5±8.6, contralateral side: 39.4±10.8; occipital lobe - ipsilateral side: 24.0±6.0, contralateral side: 26.4±6.6). The highest resting state CBF was observed in the insula (ipsilateral side: 49.2±17.4; contralateral side: 55.3±18.4). A relatively high resting state CBF was also recorded in the thalamus (ipsilateral side: 39.7±16.9; contralateral side: 41.7±14.1) and cerebellum (ipsilateral side: 41.4±12.2; contralateral side: 38.1±11.3). The highest CVR was observed in temporal lobe cortex (ipsilateral side: +27.1%; contralateral side: +26.1%) and cerebellum (ipsilateral side: +27.0%; contralateral side: +34.6%). The lowest CVR was recorded in brain stem (ipsilateral side: +20.2%; contralateral side: +22.2%) and white matter (ipsilateral side: +18.1%; contralateral side: +18.3%). All CBF values were provided in milliliters of blood per minute per 100 g of brain tissue (ml/100g/min). Resting state circulation in subjects with carotid artery stenosis is low in all analysed structures with the exception of insula and cerebellum. Acetazolamide challenge yields relatively uniform response in both hemispheres in the investigated population. Grey matter is more reactive to acetazolamide challenge than white matter or brainstem.

Human subarachnoid space width oscillations in the resting state.

Abnormal cerebrospinal fluid (CSF) pulsatility has been implicated in patients suffering from various diseases, including multiple sclerosis and hypertension. CSF pulsatility results in subarachnoid space (SAS) width changes, which can be measured with near-infrared transillumination backscattering sounding (NIR-T/BSS). The aim of this study was to combine NIR-T/BSS and wavelet analysis methods to characterise the dynamics of the SAS width within a wide range of frequencies from 0.005 to 2 Hz, with low frequencies studied in detail for the first time. From recordings in the resting state, we also demonstrate the relationships between SAS width in both hemispheres of the brain, and investigate how the SAS width dynamics is related to the blood pressure (BP). These investigations also revealed influences of age and SAS correlation on the dynamics of SAS width and its similarity with the BP. Combination of NIR-T/BSS and time-frequency analysis may open up new frontiers in the understanding and diagnosis of various neurodegenerative and ageing related diseases to improve diagnostic procedures and patient prognosis.

New aspects in assessment of changes in width of subarachnoid space with near-infrared transillumination/backscattering sounding, part 1: Monte Carlo numerical modeling.

A modified Monte Carlo method was used for numerical modeling of the propagation of near-infrared radiation (NIR) within the anatomical layers of the human head. The distribution of NIR transmission between particular anatomical layers in the measurement region (frontal tubers) of the head was obtained. The study demonstrates the effect of the cardiac pump function-dependent changes in the width of the subarachnoid space (SAS) on the intensity of the backscattered radiation. It was proved that the influence of this factor increases with increasing distance between the observation point and the location of the NIR source placed on the surface of the head. Moreover, with sufficiently small NIR detector-source distance, the contribution of the optic radiation propagated within the SAS to the total signal received is negligibly low, which gives a basis for estimation of the modulatory influence of blood circulation within the superficial skin layer on the total intensity of the backscattered radiation. The dimensions of anatomical layers used in the study are real values measured in a female patient, in whom--due to unique circumstances--it was possible to make measurements followed by recordings in clinical conditions, a situation essential for verification of the results of numerical modeling.

New aspects in assessment of changes in width of subarachnoid space with near-infrared transillumination-backscattering sounding, part 2: clinical verification in the patient.

The study presents comparison of near-infrared light propagation and near-infrared backscattered radiation power, as simulated with numerical modeling and measured live in a patient in clinical conditions with the use of the near-infrared transillumination-backscattering sounding (NIR-TBSS) technique. A unique chance for such precise comparative analysis was available to us in a clinical case of a female patient with scalp removed from one half of the head due to injury. The analysis performed indicates that the difference between the intensity of the signals in numerical modeling and live measurements is less than 4 dB. Analysis of the theoretical model also provides hints on the positioning of the two detectors relative to the source of radiation. Correctness of these predicted values is confirmed in practical application, when changes of signals received by the detectors are recorded, along with changes of the width of the subarachnoid space. What is more, the power distribution of the spectrum of near-infrared backscattered radiation returning to the detectors is confirmed in the real recording in the patient. An abridged description of the new method of NIR-TBSS is presented.

Increased inspiratory resistance affects the dynamic relationship between blood pressure changes and subarachnoid space width oscillations.

BACKGROUND AND OBJECTIVE: Respiration is known to affect cerebrospinal fluid (CSF) movement. We hypothesised that increased inspiratory resistance would affect the dynamic relationship between blood pressure (BP) changes and subarachnoid space width (SAS) oscillations. METHODS: Experiments were performed in a group of 20 healthy volunteers undergoing controlled intermittent Mueller Manoeuvres (the key characteristic of the procedure is that a studied person is subjected to a controlled, increased inspiratory resistance which results in marked potentiation of the intrathoracic negative pressure). BP and heart rate (HR) were measured using continuous finger-pulse photoplethysmography; oxyhaemoglobin saturation with an ear-clip sensor; end-tidal CO2 with a gas analyser; cerebral blood flow velocity (CBFV), pulsatility and resistive indices with Doppler ultrasound. Changes in SAS were recorded with a new method i.e. near-infrared transillumination/backscattering sounding. Wavelet transform analysis was used to assess the BP and SAS oscillations coupling. RESULTS: Initiating Mueller manoeuvres evoked cardiac SAS component decline (-17.8%, P<0.001), systolic BP, diastolic BP and HR increase (+6.3%, P<0.001; 6.7%, P<0.001 and +2.3%, P<0.05, respectively). By the end of Mueller manoeuvres, cardiac SAS component and HR did not change (+2.3% and 0.0%, respectively; both not statistically significant), but systolic and diastolic BP was elevated (+12.6% and +8.9%, respectively; both P<0.001). With reference to baseline values there was an evident decrease in wavelet coherence between BP and SAS oscillations at cardiac frequency in the first half of the Mueller manoeuvres (-32.3%, P<0.05 for left hemisphere and -46.0%, P<0.01 for right hemisphere) which was followed by subsequent normalization at end of the procedure (+3.1% for left hemisphere and +23.1% for right hemisphere; both not statistically significant). CONCLUSIONS: Increased inspiratory resistance is associated with swings in the cardiac contribution to the dynamic relationship between BP and SAS oscillations. Impaired cardiac performance reported in Mueller manoeuvres may influence the pattern of cerebrospinal fluid pulsatility.

Acute hypoxia diminishes the relationship between blood pressure and subarachnoid space width oscillations at the human cardiac frequency.

BACKGROUND: Acute hypoxia exerts strong effects on the cardiovascular system. Heart-generated pulsatile cerebrospinal fluid motion is recognised as a key factor ensuring brain homeostasis. We aimed to assess changes in heart-generated coupling between blood pressure (BP) and subarachnoid space width (SAS) oscillations during hypoxic exposure. METHODS: Twenty participants were subjected to a controlled decrease in oxygen saturation (SaO2 = 80%) for five minutes. BP and heart rate (HR) were measured using continuous finger-pulse photoplethysmography, oxyhaemoglobin saturation with an ear-clip sensor, end-tidal CO2 with a gas analyser, and cerebral blood flow velocity (CBFV), pulsatility and resistive indices with Doppler ultrasound. Changes in SAS were recorded with a recently-developed method called near-infrared transillumination/backscattering sounding. Wavelet transform analysis was used to assess the relationship between BP and SAS oscillations. RESULTS: Gradual increases in systolic, diastolic BP and HR were observed immediately after the initiation of hypoxic challenge (at fifth minute +20.1%, +10.2%, +16.5% vs. baseline, respectively; all P<0.01), whereas SAS remained intact (P = NS). Concurrently, the CBFV was stable throughout the procedure, with the only increase observed in the last two minutes of deoxygenation (at the fifth minute +6.8% vs. baseline, P<0.05). The cardiac contribution to the relationship between BP and SAS oscillations diminished immediately after exposure to hypoxia (at the fifth minute, right hemisphere -27.7% and left hemisphere -26.3% vs. baseline; both P<0.05). Wavelet phase coherence did not change throughout the experiment (P = NS). CONCLUSIONS: Cerebral haemodynamics seem to be relatively stable during short exposure to normobaric hypoxia. Hypoxia attenuates heart-generated BP SAS coupling.

Identification of Small Peptides of Acidic Collagen Extracts from Silver Carp Skin and Their Therapeutic Relevance.

BACKGROUND: Low-temperature techniques that prevent protein denaturation are being used to extract collagen from fish skin for cosmetic purposes. These extracts contain collagen with its triple helix structure preserved, as well as a number of other proteins. OBJECTIVES: The aim of the study was to investigate collagen extracts from the skin of silver carp for the presence of small-molecule peptides. MATERIAL AND METHODS: Liquid chromatography-mass spectrometry (HPLC-MS) was performed to analyze collagen extracts from silver carp skin for the presence of small-molecule peptides. RESULTS: A large number of different peptides were detected in the silver carp skin collagen extracts analyzed. Among the smaller peptides, the most abundant were those of 7-29 aminoacids originating from the following proteins: collagen Iα1, collagen Iα2, collagen Iα3, collagen VIα3, decorin, lumican, histone H2A, histone H2B and histone H4. CONCLUSIONS: The study demonstrated that, in addition to high-molecular-weight collagen proteins, acidic collagen extracts acquired from the skin of silver carp at temperatures up to 16°C also contain considerable amounts of small 7-29 amino-acid peptides. The application of these peptides could therefore be expected to result in beneficial clinical effects in patients in need of reconstructive treatment.

The Effect of Silver Carp Skin-Derived Peptides on the Activities of VEGF and Hsp70.1 Gene Transcription Promoters.

BACKGROUND: Studies conducted on human cell culture models have demonstrated that collagen-derived peptides can exert a beneficial effect in medicine. However, all these studies were conducted using animal collagen samples, most often originating from bovine or porcine skin. Currently attempts are being made to replace animal collagen with fish collagen. OBJECTIVES: The aim of the study was to compare the effect of silver carp skin-derived peptide extract on the transcriptional activities of human VEGF and hsp70.1 gene promoters inserted into the plasmids with secreted alkaline phosphatase as a reporter gene. MATERIAL AND METHODS: Changes in the activity of the promoters were investigated using a HEK293FT cell line transfected with pVEGF-SEAP or pHsp70-SEAP. The cells were cultured in dishes containing peptides separated using reverse-phase high performance liquid chromatography. RESULTS: The study demonstrated that the silver carp skin-derived peptide extract exerts both an inhibitory effect on the VEGF gene promoter and activating effect on the hsp70.1 gene promoter. Higher biological activity was recorded in the case of a freshly prepared peptide extract compared to one stored at 4°C for three months. CONCLUSIONS: The silver carp skin-derived collagen peptides influence VEGF and hsp70.1 gene promoters' transcriptional activity.