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In this investigation, two new thermophilic bacteria were isolated. The new isolates were characterized by 16S rRNA, biochemical, morphological, and physiological analyzes and the isolates were identified as Geobacillus stearothermophilus strain Gecek20 and thermophilic Anoxybacillus flavithermus strain Gecek19. Various biological activities of extracellular Ag-NPs synthesized from thermophilic G. stearothermophilus strain Gecek20 and thermophilic A. flavithermus strain Gecek19 were evaluated. The produced NPs were analyzed by SEM, SEM-EDX, and XRD analyses. The antioxidant abilities of new synthesized Ag-NPs from thermophilic G. stearothermophilus strain Gecek20 (T1-Ag-NPs) and new synthesized Ag-NPs from thermophilic A. flavithermus strain Gecek19 (T2-Ag-NPs) were studied by DPPH inhibition and metal chelating ability. The highest DPPH and metal chelating abilities of T1-Ag-NPs and T2-Ag-NPs at 200 mg/L concentration were 93.17 and 90.85%, and 75.80 and 83.64%, respectively. The extracellular green synthesized T1-Ag-NPs and T2-AgN-Ps showed DNA nuclease activity at all tested concentrations. Moreover, both new synthesized Ag-NPs had antimicrobial activity against the strains studied, especially on Gram positive bacteria. T1-Ag-NPs and T2-AgNPs also showed powerful Escherichia coli growth inhibition. The highest biofilm inhibition percentages of T1-Ag-NPs and T2-Ag-NPs against Pseudomonas aeruginosa and Staphylococcus aureus were 100.0%, respectively, at 500 mg/L.
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Anoxybacillus , Geobacillus stearothermophilus , Nanopartículas del Metal , ARN Ribosómico 16S , Plata/farmacología , Escherichia coliRESUMEN
In this research, nanoparticles derived from water extract of Centaurea solstitialis leaves were used as green adsorbent in Fenton reaction for Reactive Red 180 (RR180) and Basic Red 18 (BR18) dyes removal. At optimum operating conditions, nanoparticles proved high performance in the tested dyes removal with more than 98% of removal elimination. The free-radical scavenging, DNA nuclease, biofilm inhibition capability, antimicrobial activity, microbial cell viability, and antimicrobial photodynamic therapy activities of the iron oxide nanoparticles (FeO-NPs) derived from water and methanol extract of plant were investigated. Each of the following analysis: SEM-EDX, XRD, and Zeta potential was implemented for the prepared NPs characterization and to describe their morphology, composition and its behavior in an aqueous solution, respectively. It was found that, the DPPH scavenging activities increased when the amount of nanoparticles increased. The highest radical scavenging activity achieved with FeO-NPs derived from water extract of plant as 97.41% at 200 mg/L. The new green synthesized FeO-NPs demonstrated good DNA cleavage activity. FeO-NPs showed good in vitro antimicrobial activities against human pathogens. The results showed that both synthesized FeO-NPs displayed 100% antimicrobial photodynamic therapy activity after LED irradiation. The water extract of FeO-NPs and methanol extract of FeO-NPs also showed a significant biofilm inhibition.
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Antiinfecciosos , Nanopartículas del Metal , Nanopartículas , Humanos , Agua , Metanol , Nanopartículas/química , Antiinfecciosos/farmacología , Colorantes/química , Nanopartículas Magnéticas de Óxido de Hierro , Nanopartículas del Metal/química , Antibacterianos/farmacología , Antibacterianos/química , Pruebas de Sensibilidad MicrobianaRESUMEN
This study aimed to synthesize Ag NPs as a green catalyst for photocatalytic activity and to examine their biological activities. It was determined that they have high activity in catalytic and biological activities. The green synthesis which is an environmentally friendly and inexpensive method was used to synthesize Ag-NPs using Linum usitatissimum as a reducing agent. Transmission electron microscopy (TEM), infrared to Fourier transform infrared (FTIR) spectroscopy, UV-Visible (UV-Vis) spectroscopy, and X-ray diffraction (XRD) were used to characterize the Ag NPs. In UV-Vis examination, Ag-NPs had intense peaks in the 435 nm region. The antibacterial activity of Ag NPs was investigated, and Ag NPs showed a high lethal effect against S. aureus, E. coli, B. subtilis, and MRSA. In addition, Ag NPs were tested for anticancer activity against the HT-29 colon cancer cell line, MDA-MB-231 breast cancer cell line, healthy cell line L929-Murine Fibroblast cell Lines, and MIA PaCa-2 human pancreatic cancer cell line at various concentrations (1-160 µg/mL) and showed a high anticancerogenic properties against MDA-MB-231 cells. Ag NPs showed the ability of DNA cleavage activity. Also, the antioxidant activity of Ag NPs against DPPH was found to be 80% approximately. Furthermore, the photocatalytic activity of Ag NPs against methylene blue (MB) was determined to be 67.13% at the 180th min. In addition, it was observed that biogenic Ag NPs have high electrocatalytic activity for hydrogen peroxide (H2O2) detection. In the sensor based on Ag NPs, linearity from 1 µM to 5 µM was observed with a detection limit (LOD) of 1.323 µM for H2O2. According to these results, we conclude that the biogenic Ag NPs synthesized using Linum usitatissimum extract can be developed as an efficient biological agent as an antibacterial and anticancer also can be used as a photocatalyst for industrial wastewater treatment to prevent wastewater pollution.
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Lino , Nanopartículas del Metal , Animales , Humanos , Ratones , Peróxido de Hidrógeno , Staphylococcus aureus , Nanopartículas del Metal/química , Escherichia coli , Antibacterianos/farmacología , Antibacterianos/química , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos X , Pruebas de Sensibilidad MicrobianaRESUMEN
In this study, a new phthalonitrile derivative namely 4-[(2,4-difluorophenyl)ethynyl]phthalonitrile (1) and its metal phthalocyanines (2 and 3) were synthesized. The resultant compounds were conjugated to silver nanoparticles and characterized using transmission electron microscopy (TEM) images. The biological properties of compounds (1-3), their nanoconjugates (4-6), and silver nanoparticles (7) were examined for the first time in this study. The antioxidant activities of biological candidates (1-7) were studied by applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The highest antioxidant activity was obtained 97.47 % for 200â mg/L manganese phthalocyanine-silver nanoconjugates (6). The antimicrobial and antimicrobial photodynamic therapy (APDT) activities of biological candidates (1-7) were examined using a micro-dilution assay. The highest MIC value was obtained 8â mg/L for nanoconjugate 6 against E.â hirae. The studied compounds and their silver nanoconjugates exhibited high APDT activities against all the studied microorganisms. The most effective APDT activities were obtained 4â mg/L for nanoconjugates (5 and 6) against L.â pneumophila and E.â hirae, respectively. All the studied biological candidates displayed high cell viability inhibition activities against E.â coli cell growth. The biofilm inhibition activities of the tested biological candidates were also investigated against S.â aureus and P.â Aeruginosa. Biological candidates (1-6) can be considered efficient metal nanoparticle-based materials for multi-disciplinary biological applications.
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Antiinfecciosos , Nanopartículas del Metal , Nanoconjugados/química , Plata/farmacología , Plata/química , Nanopartículas del Metal/química , Staphylococcus aureus , Escherichia coli , Antiinfecciosos/farmacología , Antibacterianos/farmacología , Antibacterianos/química , Pruebas de Sensibilidad MicrobianaRESUMEN
Antibacterial membranes have attracted researchers' interest in recent years as a possible approach for dealing with biofouling on the membrane surface. This research aims to see if blending AZ63 Mg alloy into a polyethersulphone (PES) membrane can improve antifouling and separation properties. The composite membranes' pure water flux continued to increase from pristine PES to PES/AZ63 2.00 wt%. The results showed that PES/AZ63 2.00 wt% membrane supplied the highest permeate flux of E. coli. The steady-state fluxes of AZ63 composite membranes were 113.24, 104.38 and 44.79 L/m2h for PES/AZ63 2.00 wt%, 1.00 wt%, and 0.50 wt%, respectively. The enhanced biological activity of AZ63 was studied based on antioxidant activity, DNA cleavage, antimicrobial, anti-biofilm, bacterial viability inhibition and photodynamic antimicrobial therapy studies. The maximum DPPH scavenging activity was determined as 81.25% with AZ63. AZ63 indicated good chemical nuclease activity and also showed moderate antimicrobial activity against studied strains. The highest biofilm inhibition of AZ63 was 83.25% and 71.63% towards P. aeruginosa and S. aureus, respectively. The cell viability inhibition activity of AZ63 was found as 96.34% against E. coli. The photodynamic antimicrobial therapy results displayed that AZ63 demonstrated 100% bacterial inhibition when using E. coli.
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Incrustaciones Biológicas , Incrustaciones Biológicas/prevención & control , Escherichia coli , Staphylococcus aureus , Membranas Artificiales , Antibacterianos/farmacología , Antibacterianos/químicaRESUMEN
The search for novel antimicrobial agents is of huge importance. Nanomaterials can come to the rescue in this case. The aim of this study was to assess the cytotoxicity and antimicrobial effects of rare-earth-based orthovanadate nanoparticles. The cytotoxicity against host cells and antimicrobial activity of LaVO4:Eu3+ and GdVO4:Eu3+ nanoparticles were analyzed. Effects of nanomaterials on fibroblasts were assessed by MTT, neutral red uptake and scratch assays. The antimicrobial effects were evaluated by the micro-dilution method estimating the minimum inhibitory concentration (MIC) of nanoparticles against various strains of microorganisms, DNA cleavage and biofilm inhibition. GdVO4:Eu3+ nanoparticles were found to be less toxic against eukaryotic cells compared with LaVO4:Eu3+. Both nanoparticles exhibited antimicrobial activity and the highest MIC values were 64 mg/L for E. hirae, E. faecalis and S. aureus shown by GdVO4:Eu3+ nanoparticles. Nanoparticles demonstrated good DNA cleavage activity and induction of double-strand breaks in supercoiled plasmid DNA even at the lowest concentrations used. Both nanoparticles showed the biofilm inhibition activity against S. aureus at 500 mg/L and reduced the microbial cell viability. Taken the results of host toxicity and antimicrobial activity studies, it can be assumed that GdVO4:Eu3+ nanoparticles are more promising antibacterial agents compared with LaVO4:Eu3+ nanoparticles.
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Antiinfecciosos , Nanoestructuras , Antibacterianos/farmacología , Antiinfecciosos/farmacología , Pruebas de Sensibilidad Microbiana , Staphylococcus aureus , Vanadatos/farmacologíaRESUMEN
Nanotechnology can be applied to design antibacterial agents to combat antibiotic resistance. The aim of the present study was to assess the antimicrobial effects and cytotoxicity of GdYVO4:Eu3+ nanoparticles (NPs). Biofilm inhibition activity, antimicrobial activity, bacterial viability inhibition and DNA cleavage activity of GdYVO4:Eu3+ NPs were studied. In addition, the impact of GdYVO4:Eu3+ NPs on the mitochondrial membrane potential (ΔΨM) of host immune cells and, hence, their apoptosis was analyzed by JC-1 staining using flow cytometry. GdYVO4:Eu3+ NPs demonstrated good antimicrobial, cell viability inhibition and DNA cleavage activities. In addition, GdYVO4:Eu3+ NPs showed good biofilm inhibition activity against S. aureus and P. aeruginosa and inhibition percentages were 89.15% and 79.54%, respectively. However, GdYVO4:Eu3+ NPs promoted mitochondrial depolarization and apoptosis of leukocytes at high concentrations. GdYVO4:Eu3+ nanoparticles are promising antibacterial agents. However, more efforts should be exerted to ensure their safety.
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Antiinfecciosos , Nanopartículas del Metal , Nanopartículas , Antibacterianos/farmacología , Antiinfecciosos/farmacología , Biopelículas , Pruebas de Sensibilidad Microbiana , Pseudomonas aeruginosa , Staphylococcus aureusRESUMEN
Dermatophytes are among the most successful fungal pathogens in humans, but their virulence mechanisms have not yet been fully characterized. Dermatophytic fungi secrete proteases in vivo, which are responsible for fungal colonization and degradation of the keratinized tissue during infection. In the present study, we used PCR to investigate the presence of genes encoding fungalysins (MEP) and subtilisins (SUB) in three dermatophyte species whose incidence is increasing in Europe: the anthropophilic Trichophyton rubrum (n = 58), zoophilic Microsporum canis (n = 33), and Trichophyton benhamiae (n = 6). MEP2 and SUB4 genes were significantly correlated with T. rubrum; MEP3 and SUB1 were mostly frequently harbored by M. canis; and MEP1, 2, and 4 and SUB3-7 were most frequently harbored by T. benhamiae isolates (p < 0.05). Furthermore, MEP1-5 and SUB1-3 genes were significantly more prevalent among human clinical isolates of M. canis (n = 17) than among asymptomatic cat isolates of M. canis (n = 16; p < 0.05). Unidentified MEP and/or SUB genes in some isolates in the current study may suggest that other gene repertoires may be involved in the degradation of keratin. The presented analysis of the incidence of MEP and SUB virulence genes in three dermatophyte species of diverse origins provides an insight into the host-fungus interaction and dermatophyte pathogenesis.
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Arthrodermataceae/genética , Arthrodermataceae/patogenicidad , Péptido Hidrolasas/metabolismo , Subtilisina/metabolismo , Animales , Arthrodermataceae/metabolismo , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Humanos , Péptido Hidrolasas/genética , Subtilisina/genética , Trichophyton/genética , Trichophyton/metabolismo , Trichophyton/patogenicidadRESUMEN
HBV is a DNA virus and the causative agent of hepatitis B infection. Hepatitis B is a contagious disease and is still a major health problem all over the world. When the infection become chronic, it may cause serious diseases such as fibrosis, cirrhosis and/or hepatocellular carcinoma. Interferon/pegylated interferon by intravenous route and nucleoside/nucleotide (NA) analogues such as lamivudine, adefovir, entecavir, telbivudine and tenofovir given by oral route are used in the treatment. Antivirals given by oral route are mostly preferred in the treatment. However, because of the replication strategy and biological properties of HBV, mutations that cause antiviral resistance against these drugs can occur at different rates, although they can vary from drug to drug over time. It is possible that drug resistant virus may transmit from patient to healthy individuals. Therefore, there is a possibility of infection with drug-resistant HBV before treatment. Antiviral resistance mutations are divided into four categories; i) Nucleos(t)ide analog resistance (NAr)-related mutations, ii) primary drug resistance mutations, iii) secondary/compensatory mutations, iv) putative antiviral resistance mutations and pre-treatment variations. Recent studies have focused particularly on putative mutations and pre-treatment variations. The aim of this study was to better understanding of the antiviral resistance profiles of chronic hepatitis B (CHB) patients treated and untreated with NA, and help to prevent unnecessary drug use, minimize the side effects and economic damages. A total of 124 patients who have received nucleoside analog (NA) drug treatments (n= 72) and patients without NA treatment (n= 52) were included in the study. Viral DNA was isolated from the plasma samples of the patients. A DNA fragment, which is 551 bp, was amplified and sequenced including the binding side of all nucleoside analogs containing the B, C and D domains located in the reverse transcriptase region in the HBV genome. Different types of mutations were detected in 13 (18.05%) of 72 treated patients and in 18 (34.61%) of 52 untreated patients (p< 0.05). Primary drug resistance mutations such as rtI169T, rtA181T/V, rtT184A/C/F/G/I/L/M/S, rtA194T, rtS202C/G/I, rtM204I/V/S, rtN236T, rt M250I/L/V and rtV173L were not detected in any of the patient samples. However, potential drug resistance mutations such as rtR164R, rtG165D/A, rtG172Q, rtS176N, rtF178V, rtA181G, rtS185N/G/C, rtV207M, rtQ215H/S, rtL231V, rtI233K, rtN238S, rtV253T, rtC256G/S and rtI266R/V were detected in untreated patient samples in B, C, D and D domains of reverse transcriptase region. Our results have suggested that the detection of pretreatment variations could be helpful for choosing the correct antiviral drug for the better treatment management.
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Farmacorresistencia Viral , Virus de la Hepatitis B , Hepatitis B Crónica , Antivirales/farmacología , Farmacorresistencia Viral/genética , Virus de la Hepatitis B/efectos de los fármacos , Virus de la Hepatitis B/genética , Hepatitis B Crónica/tratamiento farmacológico , Hepatitis B Crónica/virología , Humanos , Mutación , Inhibidores de la Transcriptasa Inversa/farmacologíaRESUMEN
Cryptococcus neoformans is a basidiomycetous encapsulated yeast that can cause life-threatening infections in immunosuppressed humans and animals. C.neoformans/Cryptococcus gattii infections are considered to be acquired via inhalation of aerosolized particles from the environment. Avian guano, decaying tree hollows and soil are known as environmental niches. In recent years, colonization of the woody structures of different trees such as Eucalyptus camaldulensis, Tamarix hispida, Platanus orientalis and Punica granatum has been reported in the environmental study of the western Anatolian region. Based on the results of previous studies, our country may have intensive Cryptococcus colonization niches in the western regions. The aim of this study was to investigate the presence of the colonization of C.neoformans niche in chestnut (Castanea spp.) trees on higher altitudes. In the study, the colonization of C.neoformans was screened on chestnut trees (Castanea spp.) in Aydin-Ödemis-Denizli geographical area. This area consists of mountainous terrain between the fertile plain formed by two major rivers.This region is one of the widespreading areas of chestnut farming in Anatolia. Two hundred and fourteen chestnut trees that had deep fissures or trunk hollows were screened during mid-summer 2017. A swabbing technique was used, and all samples were cultured on Staib agar medium containing biphenyl and antibiotics. Cultures were checked for ten days for suspicious brown colonies. Suspicious yeast colonies were tested for the identification of pathogenic Cryptococcus by conventional methods and canavanine-glycine-bromothymol agar reactions. ITS 1-4 primers were used for strain PCR tests. We determined the mating type and serotypes by PCR analysis of the STE20 genes using STE20 (Aa), STE20 (Aα), STE20 (Da), and STE20 (Dα) primers. V8 agar medium was used for mating cultivation. Only one (0.47%) strain of C.neoformans was isolated from 214 screened trees. This strain was confirmed by ITS 1-4 sequencing. The serotype A MATα mating type was observed. Basidium, basidiospores and clamp connections in hyphal structure were noted with MATα mating on V8 agar medium. In this study, the first C.neoformans isolate from a chestnut tree (Castanea sativa) was determined from Denizli region. Further studies of distribution of human pathogenic Cryptococcus will be helpful to determine the risk areas for the living organisms in our region.
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Criptococosis , Cryptococcus neoformans , Microbiología Ambiental , Fagaceae , Árboles , Cryptococcus neoformans/aislamiento & purificación , Fagaceae/microbiología , Árboles/microbiología , TurquíaRESUMEN
Candida parapsilosis, although a human commensal, acts as an opportunistic pathogen associated with nosocomial infections, with a rising incidence worldwide. Its ecological characteristics are poorly understood. Human-made environments within dwellings, such as dishwashers and water distribution systems, represent major sources of fungi such as C. parapsilosis. Here, we investigated the presence of members of the C. parapsilosis complex in 99 washing machines in various dwellings in the city of Mersin, Turkey. We sampled three sites in each washing machine: (i) the washing powder drawers, (ii) fabric softener drawers, and (iii) rubber seals around the washing machine doors. Additionally, we recorded the type of cleanser used by each customer. Of note, 25.3% of sampled washing machines harbored C. parapsilosis strains, later identified as the members of the C. parapsilosis sensu stricto via internal transcribed spacer (ITS) sequencing. Out of the 29 isolates obtained, biofilm-forming ability and proteinase and esterase activities were recorded in 14, 11, and 4 of the isolates, respectively. Our results suggest that the washing machines investigated abundantly harbored C. parapsilosis sensu stricto; however, no single preferred isolation site or association with cleanser type was observed (P > .05). Furthermore, C. parapsilosis isolates grew at temperatures ranging from 10°C to 37°C, at pH values ranging from 4 to 10, and were found to tolerate 5-10% NaCl. Domestic laundry appliances as a potential source of C. parapsilosis infections are discussed.
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Candida parapsilosis/aislamiento & purificación , Microbiología Ambiental , Contaminación de Equipos , Artículos Domésticos , Candida parapsilosis/enzimología , Candida parapsilosis/genética , Candida parapsilosis/crecimiento & desarrollo , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Detergentes , Ecosistema , Humanos , Técnicas de Tipificación Micológica , Infecciones Oportunistas/microbiología , Análisis de Secuencia de ADN , TurquíaRESUMEN
It is known that heavy metal containing nanomaterials can easily prevent the formation of microbial cultures. The emergence of new generation epidemic diseases in the last 2 years has increased the importance of both personal and environmental hygiene. For this reason, in addition to preventing the spread of diseases, studies on alternative disinfectant substances are also carried out. In this study, the antibacterial activity of nanoflower and nanocube, which are easily synthesized and nanoparticle species containing iron, were compared. The antioxidant abilities of new synthesized NF@FeO(OH) and NC@α-Fe2O3 were tested by DPPH scavenging activity assay. The highest DPPH inhibition was achieved with NC@α-Fe2O3 as 71.30% at 200 mg/L. NF@FeO(OH) and NC@α-Fe2O3 demonstrated excellent DNA cleavage ability. The antimicrobial capabilities of NF@FeO(OH) and NC@α-Fe2O3 were analyzed with micro dilution procedure. In 500 mg/L, the antimicrobial activity was 100%. In addition to these, the biofilm inhibition of NF@FeO(OH) and NC@α-Fe2O3 were investigated against S. aureus and P. aeruginosa and it was found that they showed significant antibiofilm inhibition. It is suggested that additional studies can be continued to be developed and used as an antibacterial according to the results of the nanoparticles after various toxicological test systems. Supplementary Information: The online version contains supplementary material available at 10.1007/s13204-023-02822-5.
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Disinfection can be accomplished by adding external chemical agents to kill harmful microorganisms or by removing them using membranes. However, most chemicals are toxic for humans and animals if it is consumed above a certain concentration. Likewise, membranes have fouling problems. The aim of this study is to investigate the effect of diode laser, which is an environmentally friendly application, on pathogenic microorganisms such as Escherichia coli (ATCC 10536), Staphylococcus aureus (ATCC 6538) and Candida albicans. To reveal the effect of diode laser on aforementioned, various parameters have been studied on how diode laser type, laser irradiation time, laser power density, laser penetration efficiency and biofilm inhibition affect microorganisms. As a result of the study, it was observed that the blue laser was more effective than red and green lasers, and the inhibition rates for 15â min at 0.36 W/cm2 laser power density were 65.9% > 34.52% > 43.63% for S. aureus, E. coli and C. albicans, respectively. After 30â min of blue laser irradiation, the microbial growth inhibitions were found as 85.39%, 41.18% and 54.55% for S. aureus, E. coli and C. albicans, respectively. The highest biofilm inhibition was 94.61% when S. aureus cells were exposed to blue laser irradiation for 60â min. The microbial growth kinetics on three microorganisms were tested by using at 0.54â W/cm2 laser power density for 28 h, and there were not observed any microbial development in microbial cultures. Moreover, blue laser irradiation was successfully disinfected wastewater and natural milk at 0.54â W/cm2 laser power density.
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Láseres de Semiconductores , Staphylococcus aureus , Humanos , Animales , Staphylococcus aureus/efectos de la radiación , Desinfección , Aguas Residuales , Leche , Escherichia coli/efectos de la radiación , Bacterias GrampositivasRESUMEN
The control over bacterial diseases requires the development of novel antibacterial agents. The use of antibacterial nanomedicines is one of the strategies to tackle antibiotic resistance. The study was designed to assess the antimicrobial activity of cerium oxide (CeO2 ) nanoparticles (NP) of two different sizes (CeO2 NP1 [1-2 nm] and CeO2 NP2 [10-12 nm]) and their cytotoxicity towards eukaryotic cells. The antimicrobial activity, effects of nanoparticles on DNA cleavage, microbial cell viability, and biofilm formation inhibition were analyzed. The impact of cerium oxide nanoparticles on eryptosis of erythrocytes was estimated using annexin V staining by flow cytometry. The newly synthesized CeO2 NP1 and CeO2 NP2 displayed moderate antimicrobial activities. CeO2 NP1 and CeO2 NP2 exhibited single-strand DNA cleavage ability. CeO2 NPs were found to show 100% microbial cell viability inhibition at a concentration of 500 mg/L. In addition, CeO2 NP1 and CeO2 NP2 inhibited the biofilm formation of S. aureus and P. aeruginosa. Larger cerium oxide nanoparticles were found to be less toxic against erythrocytes compared with the smaller ones. CeO2 nanoparticles demonstrate moderate antimicrobial activity and low cytotoxicity towards erythrocytes, which make them promising antibacterial agents.
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Antiinfecciosos , Cerio , Nanopartículas del Metal , Nanopartículas , Staphylococcus aureus , Antibacterianos/farmacología , Antiinfecciosos/farmacología , Cerio/farmacologíaRESUMEN
In the present study, Ag nanoparticles (AgNPs) were synthesized from Salvia officinalis and Thymus vulgaris, known as phytotherapy plants. The obtained silver nanoparticles were characterized using SEM, XRD, FTIR, and UV/Vis spectra. The antioxidant capacities of Salvia officinalis-mediated AgNP (SO-AgNP) and Thymus vulgaris-mediated AgNP (TV-AgNP) were analyzed in vitro using 1,1-diphenyl-2-picrylhydrazyl and iron chelating activity assays. DPPH activities were 83.74% and 57.17% for SO-AgNP and TV-AgNP at concentration 200 mg/L, respectively. Both green synthesized AgNPs exhibited good iron chelating activity. In addition, the DNA cleavage activities of SO-AgNPs and TV-AgNP were investigated with agarose gel electrophoresis technique. SO-AgNPs and TV-AgNP showed single-strand DNA cleavage activity. AgNPs showed that the SO-AgNP and TV-AgNp were effective against bacteria and fungi, and antimicrobic activities were assessed as minimal inhibition concentration (MIC). Remarkably, green synthesized AgNPs showed highly effective cell viability and biofilm inhibition effect. AgNPs also demonstrated slightly antimicrobial photodynamic activity after LED irradiation.
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New asymmetric Si(IV)Pc (1), monomeloxicammonotriethyleneglycolmonomethylether (phthalocyaninano)silicone, axially ligated with meloxicam as a non-steroidal anti-inflammatory drug (NSAID), or triethylene glycol monomethyl ether and symmetric Si(IV)Pc (2), diclofenac(phthalocyaninano)silicone, axially ligated with two diclofenac as NSAID, were synthesized and characterized as antioxidant and antimicrobial agents together with polyoxo-SiPc (3), ditriethyleneglycolmonomethylether(phthalocyaninano)silicone, and SiPc(OH)2 (4), dihydroxy(phthalocyaninano)silicone. The photophysical and photochemical properties of these compounds were investigated. Then, antioxidant assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferrous ion chelating activities, were performed for these Si(IV) phthalocyanine derivatives (1, 2, 3 and 4). The highest DPPH scavenging activity of 73.48% was achieved with compound 2 and the highest ferrous chelating ability of 66.42% was obtained with compound 3. The results of the antioxidant assays indicated that Pc derivatives 1, 2 and 3 have remarkable superoxide radical scavenging activities, and moderate 2,2-diphenyl-1-picrylhydrazyl activities and metal chelating activities. The antimicrobial effects of the Si(IV) phthalocyanine compounds were studied against six pathogenic bacteria and two pathogenic microfungi. The results for the antimicrobial activity of these compounds indicated that Enterococcus faecalis (ATCC 29212) was the most sensitive microorganism and Pseudomonas aeruginosa (ATCC 27853) and Legionella pneumophila subsp. pneumophila (ATCC 33152) were the most resistant microorganisms against the tested compounds. The DNA cleavage ability and microbial cell viability of these compounds were studied. The studied compounds demonstrated excellent DNA nuclease activity and exhibited 100% cell viability inhibition at 500 mg L-1. Also, the antimicrobial photodynamic therapy of the compounds was tested against Escherichia coli (ATCC 25922) and significant photodynamic antimicrobial activity was observed. In addition, the effect of phthalocyanines on biofilm inhibition produced by Staphylococcus aureus (ATCC 25923) was also tested and 3 showed excellent biofilm inhibition of 82.14%.
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Antiinfecciosos , Antioxidantes , Antibacterianos/química , Antiinfecciosos/química , Antiinflamatorios/farmacología , Antiinflamatorios no Esteroideos/farmacología , Antioxidantes/química , Quelantes/farmacología , Diclofenaco , Escherichia coli , Indoles/química , Indoles/farmacología , Isoindoles , Pruebas de Sensibilidad Microbiana , Siliconas/farmacologíaRESUMEN
The emergence of nanoscience and its effect on the development of diverse scientific fields, particularly materials chemistry, are well known today. In this study, a new di-substituted phthalonitrile derivative, namely 4,5-bis((4-(dimethylamino)phenyl)ethynyl)phthalonitrile (1), and its octa-substituted metal phthalocyanines {M = Co (2), Zn (3)} were prepared. All the newly synthesized compounds were characterized using a number of spectroscopic approaches, including FT-IR, mass, NMR, and UV-vis spectroscopy. The resultant compounds modified the surface of the gold nanoparticles (NG-1-3). Characterization of the newly synthesized conjugates was carried out by transmission electron microscopy. The antioxidant activity of compounds 1-3, NG-1-3, and NG was evaluated using the DPPH scavenging process and the highest radical scavenging activity was obtained with compounds 1, NG-1, 2, and NG-2 (100%). The antimicrobial activity of compounds 1-3, NG-1-3, and NG was studied using a microdilution assay and the most effective antimicrobial activity was obtained for NG-3 against all the tested microorganisms. The newly synthesized compounds demonstrated high DNA cleavage activity. Compounds 1-3, NG-1-3, and NG significantly inhibited the microbial cell viability of E. coli and exhibited perfect antimicrobial photodynamic therapeutic activity with 100% inhibition after 20 min LED irradiation. Besides, the biofilm inhibition activity of compounds 1-3, NG-1-3, and NG on the growth of S. aureus and P. aeruginosa were examined and compounds 1-3 and NG-1-3, especially NG-1-3, displayed high biofilm inhibition activities.
Asunto(s)
Antibacterianos/farmacología , Antioxidantes/farmacología , Complejos de Coordinación/farmacología , Escherichia coli/efectos de los fármacos , Antibacterianos/síntesis química , Antibacterianos/química , Antioxidantes/síntesis química , Antioxidantes/química , Compuestos de Bifenilo/antagonistas & inhibidores , Supervivencia Celular/efectos de los fármacos , Complejos de Coordinación/síntesis química , Complejos de Coordinación/química , Isoindoles/química , Isoindoles/farmacología , Nanopartículas del Metal , Metales Pesados/química , Metales Pesados/farmacología , Pruebas de Sensibilidad Microbiana , Picratos/antagonistas & inhibidoresRESUMEN
In this study, synthesis of silver nanoparticles (AgNPs) was carried out utilizing the red and green parts of the pistachio hulls then their several biological activities were investigated. The DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) activities of the AgNPs synthesized from red pistachio hulls extracts (PhR-AgNPs) and green pistachio hulls extracts (PhG-AgNPs) were investigated. The DPPH scavenging capability at 200 mg/L concentration of PhR-AgNPs was around 93.01% however PhG-AgNPs displayed 91.00%. The synthesized PhR-AgNPs and PhG-AgNPs acted on the E. coli plasmid DNA, causing its complete degradation and exhibiting effective chemical nuclease activity. Furthermore, PhR-AgNPs and PhG-AgNPs showed quite good antimicrobial activity against the studied strains with a range of the minimum inhibition concentration (MIC) of 8-16 mg/L. Moreover, it was observed that both pistachio hulls coated with AgNPs were highly effective in inhibiting the biofilm generation studied strains. Moreover, PhR-AgNP and PhG-AgNP displayed a completely inhibition effect on cellular viability of E. coli with 100% at 125 mg/L.
Asunto(s)
Nanopartículas del Metal , Pistacia , Antibacterianos/farmacología , Escherichia coli , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/farmacología , Plata/farmacologíaRESUMEN
In this study, the electrooxidation (EO) and membrane processes were used for chemical oxygen demand (COD) and total phenol (TPh) removal from wet scrubber wastewater (WSW). EO experiments were carried out using Al, Fe, SS, Ti, graphite, active carbon cloth electrodes and Box-Behnken design were used for optimization of maximum COD and TPh removal efficiency. Moreover, membrane filtration experiments were conducted to EO process using nanofiltration (NF270) and reverse osmosis membranes (SW30 and BW30). The maximum COD (55%) and TPh (50%) removal efficiency was achieved at pH of 8, 150 A/m2 current density, and 180 min reaction time in EO process. Membrane filtration results showed that COD removal efficiency was the highest for SW30 membrane (95.18%) compared to BW30 (91.15%) and NF270 (80.11%) membranes. TPh removal efficiency in the NF270, BW30, and SW30 membranes was 27.08%, 96.06%, and 98.02%, respectively. The effect of microbial cell viability of the raw and treated wet scrubber wastewater after electrooxidation and membrane filtration was also investigated using E. coli. In addition to these, biofilm inhibition of the raw wet scrubber wastewater and the treated WSW after EO and membrane filtration were tested and the highest biofilm inhibition was found as 76.43% and 72.58% against S. aureus and P. aeruginosa, respectively, in 1/20 diluted samples of the raw WSW. This study suggests that the integrated process using EO and pressure-driven membrane methods are an efficient strategy for COD and TPh removal from WSW.
Asunto(s)
Grafito , Contaminantes Químicos del Agua , Purificación del Agua , Análisis de la Demanda Biológica de Oxígeno , Electrodos , Escherichia coli , Filtración , Residuos Industriales/análisis , Fenol , Fenoles , Staphylococcus aureus , Eliminación de Residuos Líquidos/métodos , Aguas Residuales , Purificación del Agua/métodosRESUMEN
This study presents the preparation of a novel tetra-substituted phthalonitrile (1), namely, 3,6-bis(hexyloxy)-4,5-bis(4-(trifluoromethoxy)phenoxy)phthalonitrile (1) and its metal-free (2)/metal {M = Zn (3), Cu (4), Co (5), Lu(CH3COO) (6), Lu (7)} phthalocyanines. A series of various spectroscopic methods (UV-vis, FT-IR, mass, and 1H NMR spectroscopy) were performed for the characterization of the newly synthesized compounds. The potential of compounds 2, 3, and 6 as photosensitizing materials for photodynamic and sonophotodynamic therapies was evaluated by photophysical, photochemical, and sonochemical methods. The highest singlet quantum yields were obtained for the zinc phthalocyanine derivative 3 by performing photochemical and sonochemical methods. In addition, several biological activities of the new compounds 1-7 were investigated. The newly synthesized phthalocyanines exhibited excellent DPPH scavenging activity and also DNA nuclease activity. The antimicrobial activity of the new compounds was evaluated by the disc diffusion assay. Effective microbial cell viability inhibition was observed with phthalocyanine macromolecules. The photodynamic antimicrobial therapy of the phthalocyanines showed 100% bacterial inhibition when compared to the control. They also exhibited significant biofilm inhibition activity against S. aureus and P. aeruginosa. These results indicate that new phthalocyanines are promising photodynamic antimicrobial therapies for the treatment of infectious diseases.