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2.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 48(4): 526-530, 2017 Jul.
Artículo en Zh | MEDLINE | ID: mdl-28752969

RESUMEN

OBJECTIVE: To investigate the protective effects of kaempferol on rat renal mesangial cells under high glucose condition and explore its mechanism. METHODS: The HBZY-1 cells were divided into normal glucose group (5.5 mmol/L), high glucose group (25 mmol/L), 10 µmol/L kaempferol+high glucose group, and 30 µmol/L kaempferol+high glucose group. Cell proliferative ability was measured by MTT; cell cycle was analyzed by flow cytometry; mRNA and protein levels were determined by Real-time PCR and Western blot, respectively. RESULTS: Kaempferol had no effect on the proliferative ability of rat renal mesangial cells under normal glucose (5.5 mmol/L) condition. High glucose (25 mmol/L) enhanced the cell proliferative ability, and this effect was antagonized by kaempferol (10-30 µmol/L) treatment. High glucose reduced the cell population at G0/G1 phase with an associated increase in S phase, and had no effect on G2/M phase; and kaempferol treatment restored high glucose-induced changes in cell cycle. Kaempferol also prevented high glucose-induced increase in fibronectin and connective tissue growth factor mRNA and protein expression levels. Kaempferol also prevented high glucose-induced increase in fibronectin and connective tissue growth factor mRNA and protein expression levels. Further, high glucose caused an increase in protein level of phosphorylated p38 mitogen-activated protein kinases (p38 MAPK), which was antagonized by kaempferol treatment. CONCLUSION: Our results suggest that kaempferol exerts its protective effect on rat renal mesangial cells under high glucose condition via p38 MAPK signaling pathway.


Asunto(s)
Proliferación Celular , Glucosa , Quempferoles/farmacología , Células Mesangiales/efectos de los fármacos , Animales , Ciclo Celular , Células Cultivadas , Factor de Crecimiento del Tejido Conjuntivo/metabolismo , Fibronectinas/metabolismo , Ratas , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo
3.
Medicine (Baltimore) ; 99(50): e23507, 2020 Dec 11.
Artículo en Inglés | MEDLINE | ID: mdl-33327292

RESUMEN

BACKGROUND: The pathogenesis of diabetic peripheral neuropathy is more complex and it is not yet clear, but studies have shown that microangiopathy and oxidative stress responses are closely related to their pathogenesis. At present, the treatment of improving microcirculation and antioxidant stress is mainly used in clinical. Alprostadil is a commonly used vasodilator, and alpha lipoic acid is an antioxidant, which can effectively reduce oxidative stress responses and delay the progression of diabetes mellitus and its complications. However, there is a lack of evidence-based medical evidence for alprostadil combined with alpha lipoic acid in the treatment of diabetic peripheral neuropathy, and this article aims to understand the clinical effectiveness and safety of alprostadil combined with alpha lipoic acid in the treatment of diabetic peripheral neuropath by a meta-analysis of published randomized controlled trials. METHODS: In this study, we obtain the relevant literature by retrieving 8 electronic databases, including PubMed, EMBASE, Web of Science, the Cochrane Library, CBM, CNKI, VIP, and WanFang Database. Retrieving a randomized controlled study of alprostadil combined with alpha lipoic acid in the treatment of diabetic peripheral neuropath, while the language of the literature is restricted and it only includes Chinese and English literature. For the publication of literature, the time is from the beginning of the database to August 31, 2020. In the English database, using the retrieval method of subject word combined free word. The two researchers read the titles and abstracts of all the literature independently based on the inclusion and exclusion criteria. If it cannot be determined whether the literature is included by reading the title and abstract, then download and read the full text of the literature. If there is a dispute between the two researchers about the literature, so it should discuss the dispute with the third researcher in order to reach a conclusion. Using the bias risk assessment tool of randomized controlled trials in Cochrane systematic review to evaluate the bias risk of the included literature; Using RevMan 5.3 software to conduct statistical analysis; Using funnel plot analysis to analyze the situation of literature publication bias. RESULTS: This study will provide a high-quality evidence on the effects of hydrolyzed protein formula milk on gastrointestinal diseases and physical development of premature infants. CONCLUSION: This study will draw reliable evidence-based medical evidence for alprostadil combined with Alpha lipoic acid in the treatment of diabetic peripheral neuropathy, thus providing help for the clinical treatment of diabetic peripheral neuropathy. REGISTRATION NUMBER: Open Science Framework (OSF), registration number: DOI 10.17605/OSF.IO/7S46G.


Asunto(s)
Alprostadil , Antioxidantes , Diabetes Mellitus Tipo 2 , Neuropatías Diabéticas , Ácido Tióctico , Vasodilatadores , Humanos , Alprostadil/administración & dosificación , Alprostadil/uso terapéutico , Antioxidantes/administración & dosificación , Antioxidantes/uso terapéutico , Neuropatías Diabéticas/tratamiento farmacológico , Quimioterapia Combinada , Ácido Tióctico/administración & dosificación , Ácido Tióctico/uso terapéutico , Vasodilatadores/administración & dosificación , Vasodilatadores/uso terapéutico , Revisiones Sistemáticas como Asunto , Metaanálisis como Asunto
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