[The change rules and correlation between specific leaf weight and dry weight of rhizoma of Atractylodes macrocephala].

OBJECTIVE: To study the change rules of specific leaf weight, dry weight of rhizoma and the drying rate of annual and biennial Atractylodes macrocephala. METHODS: We measured the specific leaf weight and dry weight of rhizoma, and accounted the drying rate and correlation by dugging the annual and biennial plants each 15 days. There were 3 rhizoma types of biennial Atractylodes macrocephala with Drumstick/Frog type, Er-cha type and Wu-zi type. RESULTS: It showed the curve of specific leaf weight of annual plant was "N" style, which of biennial plant was linear. The dry weight and the drying rate of all rhizoma types increased point-blank during the whole growth period. CONCLUSION: The correlation between specific leaf weight and dry weight of rhizoma is obvious in biennial Atractylodes macrocephala but is contrary in annual Atractylodes macrocephala.

[Study on the soft-wood cutting of Atractylodes macrocephala].

OBJECTIVE: The research analyzed the effect of different elements on soft-wood cutting of Atractylodes macrocephala. METHODS: The experiment was studied by L9 (3(4)) orthogonal design with 3 factors and 3 levels. The research confirmed the best project of soft-wood cutting of Atractylodes macrocephala by using variance analysis and line grading law. RESULTS: The maximum livability and rooting rate were both 86.67% when it was treated with GuoGuangShengGen of 2,000 mg/L, 20 s, the ratio of turf to perlite was 1:3 and the matrix was the most favourable rate of soft-wood cutting of Atractylodes macrocephala. CONCLUSION: This method can improve Atractylodes macrocephala cutting suruival rate and provide the theoretical basis for cutting.

[Study on tissue culture and rapid propagation of Atractylodes macrocephala].

A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz.. The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation. MS + BA 1.0 mg/L + NAA 0.3 mg/L + GA3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also. MS + BA 3.0 mg/L + IBA 0.5 mg/L was suitable for the proliferation of axillary buds, with an induction rate of 95%. 1/2MS + IBA 0.1 - 0.5 mg/L was optimum for rooting, with a rooting percentage more than 90%. The survival rate of transplanted plantlets was more than 90%.