RESUMEN
OBJECTIVE: The purpose of this pilot study was to assess the functional outcome of patients with pelvic fracture after Lushan earthquake using a newly developed tool based on the International Classification of Functioning, Disability and Health (ICF). METHODS: Ten patients diagnosed as pelvic fracture were recruited in this study from April 20th to 30th, 2013 in West China Hospital, Sichuan University. Data were collected using the international comprehensive version of ICF Core Set for patients with musculoskeletal conditions. Descriptive statistics analysis were used. RESULTS: Pelvic fracture caused by Lushan earthquake lead to a series of severe injuries complicated with multiple organ dysfunction. In the assessment of body functions, almost all the patients with pelvic fracture showed impairments including energy and drive, sleep, sensation of pain, mobility and repair function of skin. In the assessment of body structures, the damages were mainly distributed in pelvic region, lower extremity, trunk as well as areas of skin. In the assessment of activities and participations, limitations and restrictions were related to changing and maintaining basic body position, transferring, walking andself-care. CONCLUSION: ICF Core Set could provide a comprehensive, systematic and evidence-based assessments from the perspective of body function, body structure as well as activities and participations for patients with pelvic fracture after Lushan earthquake. Future study with large sample size is needed to validate the assessment of ICF Core Set for patients with musculoskeletal injuries after earthquake and other natural disasters.
Asunto(s)
Evaluación de la Discapacidad , Terremotos , Fracturas Óseas/patología , Pelvis/patología , China , Desastres , Humanos , Proyectos PilotoRESUMEN
This study was designed to determine the effects of pulsed electromagnetic fields (PEMF) on the mRNA expression of the receptor activator of NF-kappa-B (RANK) and carbonic anhydrase II (CA II) in ovariectomized rat osteoclast-like cells. Marrow cells were harvested from femora and tibiae of rats, from which the ovaries had been totally excised, and cultured in 6-well chamber slides. After 1 day of incubation, the marrow cells were exposed to PEMF for 3 days with 3.8 mT, 8 Hz, and 40 min per day. Osteoclast-like cells were confirmed by both tartrate resistant acid phosphatase (TRAP) stain and bone resorption assay. The expression of RANK and CA II mRNA was determined with real-time fluorescent-nested quantitative polymerase chain reaction. Compared with the sham group, the level of serum estradiol in the ovariectomized group was significantly decreased ( p < 0.05). The numbers of multinucleated, TRAP-positive osteoclast-like cells and resorption pits formed were observed. In invitro study, the expression of RANK and CA II were measured in sham, ovariectomized without PEMF, and ovariectomized with PEMF treatment. Compared with the ovariectomized (PEMF) experimental group and sham group, CA II mRNA expression was significantly increased in the ovariectomized control group ( p < 0.05, 0.01, respectively). Compared with the sham group, RANK mRNA expression was significantly increased in the ovariectomized control group ( p < 0.05). These data suggest that PEMF could regulate the expression of RANK and CA II mRNA in the marrow culture system.
Asunto(s)
Huesos/efectos de la radiación , Anhidrasa Carbónica II/genética , Campos Electromagnéticos , Osteoclastos/efectos de la radiación , ARN Mensajero/efectos de la radiación , Receptor Activador del Factor Nuclear kappa-B/genética , Fosfatasa Ácida , Animales , Biomarcadores/análisis , Biomarcadores/metabolismo , Células de la Médula Ósea/citología , Células de la Médula Ósea/metabolismo , Células de la Médula Ósea/efectos de la radiación , Resorción Ósea/fisiopatología , Huesos/citología , Huesos/metabolismo , Células Cultivadas , Regulación hacia Abajo/fisiología , Estrógenos/sangre , Estrógenos/deficiencia , Femenino , Regulación de la Expresión Génica/genética , Regulación de la Expresión Génica/efectos de la radiación , Humanos , Osteoclastos/citología , Osteoclastos/metabolismo , Osteoporosis Posmenopáusica/genética , Osteoporosis Posmenopáusica/metabolismo , Osteoporosis Posmenopáusica/terapia , Ovariectomía , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Coloración y EtiquetadoRESUMEN
OBJECTIVE: To analysis the related factors of efficacy of re-platinum chemotherapy in platinum-sensitive recurrent ovarian carcinoma. METHODS: Forty-one patients with platinum-sensitive recurrent ovarian cancer from Jan. 1998 to Oct. 2002 were reviewed, 37 factors were analyzed, including age, the optimal of primary surgery, number of chemotherapy cycles, standard chemotherapy or not, serum CA(125) level, follow up or not, relapse interval, the site and the number of relapse lesion, et al. RESULTS: Response rate of chemotherapy was 56%, the toxicity was not serious. Response rate of standard chemotherapy was 76% (19/25), not standard chemotherapy was 25% (4/16), standard chemotherapy or not was the only factor related to efficacy (P < 0.05). CONCLUSION: Re-platinum chemotherapy to platinum-sensitive recurrent ovarian carcinoma is reasonable, standard chemotherapy or not affects the response rate.