Interactions between inhalable aged microplastics and lung surfactant: Potential pulmonary health risks.

The relationship between microplastics (MPs) and human respiratory health has garnered significant attention since inhalation constitutes the primary pathway for atmospheric MP exposure. While recent studies have revealed respiratory risks associated with MPs, virgin MPs used as plastic surrogates in these experiments did not represent the MPs that occur naturally and that undergo aging effects. Thus, the effects of aged MPs on respiratory health remain unknown. We herein analyzed the interaction between inhalable aged MPs with lung surfactant (LS) extracted from porcine lungs vis-à-vis interfacial chemistry employing in-vitro experiments, and explored oxidative damage induced by aged MPs in simulated lung fluid (SLF) and the underlying mechanisms of action. Our results showed that aged MPs significantly increased the surface tension of the LS, accompanied by a diminution in its foaming ability. The stronger adsorptive capacity of the aged MPs toward the phospholipids of LS appeared to produce increased surface tension, while the change in foaming ability might have resulted from a variation in the protein secondary structure and the adsorption of proteins onto MPs. The adsorption of phospholipid and protein components then led to the aggregation of MPs in SLF, where the aged MPs exhibited smaller hydrodynamic diameters in comparison with the unaged MPs, likely interacting with biomolecules in bodily fluids to exacerbate health hazards. Persistent free radicals were also formed on aged MPs, inducing the formation of reactive oxygen species such as superoxide radicals (O2•-), hydrogen peroxide (HOOH), and hydroxyl radicals (•OH); this would lead to LS lipid peroxidation and protein damage and increase the risk of respiratory disease. Our investigation was the first-ever to reveal a potential toxic effect of aged MPs and their actions on the human respiratory system, of great significance in understanding the risk of inhaled MPs on lung health.

Identification, evolution, and expression analysis of OsBSK gene family in Oryza sativa Japonica.

BACKGROUND: As an essential component of the BR (brassinosteroid) signaling pathway, BSK (BR-signalling kinases) plays a vital role in plant growth, development, and stress regulation. There have been sporadic reports on the functions of members of this family in monocotyledonous model plant rice, but few reports have been reported on the phylogenetic analysis and gene expression profiling of the family genes. RESULTS: In this study, a total of 6 OsBSK members were identified at the genomic level by bioinformatics methods, distributed on four rice chromosomes. Through the evolution analysis of 74 BSK proteins from 22 species, it was found that BSKs originated from higher plants, were highly conserved, and could be divided into six subgroups. Among them, OsBSKs belonged to four subgroups or two significant groups. OsBSK family gene promoters contained a large number of light, abscisic acid (ABA), and methyl jasmonate (MeJA) response-related elements. At the same time, the qRT-PCR test also showed that the genes of this family were involved in response to a variety of hormones, biotic and abiotic stress treatments, and expression patterns of the family gene can be roughly divided into two categories, which were similar to the tissue expression patterns of genes in different growth stages. OsBSK1-1, OsBSK1-2, and OsBSK3 were mostly up-regulated. OsBSK2, OsBSK4, and OsBSK5 were mostly down-regulated or had little change in expression. CONCLUSIONS: This study revealed the origin and evolution of the BSK family and the farm-out of BSKs in rice growth, development, and stress response. It provides the theoretical reference for in-depth analysis of BR hormone, signal transduction, and molecular breeding design for resistance.

Study on the Role of Quartz in the Bio-Oxidation of Sulfide Minerals From Mine Solid Waste.

Sulfide-containing mine waste was oxidized to produce acid mine drainage, which lead to acidification of surrounding soil and downstream rivers and posed a threat to the surrounding environment. Quartz often coexists with sulfide minerals and affects the oxidation of sulfide minerals. In order to explore the role of quartz in the bio-oxidation of sulfide minerals in mine solid waste, the mixed minerals of quartz and sulfide minerals were bio-oxidized by Acidithiobacillus ferrooxidans. The results showed that quartz could improve the microbial activity and increase the acid production of sulfide minerals. The larger the proportion of quartz in bio-oxidation of sulfide minerals, the less the production of secondary minerals such as jarosite, and the larger the leaching amount of iron and sulfate. This research provides new ideas for speeding up the bio-oxidation of sulfide mineral to remove iron and sulfate. It provides a new way to solve acid pollution caused by oxidation of sulfide minerals.

Study on Tailings Covering System Constructed by Geological Polymerization of Mine Waste, Part 1: Material Characterization and Cover Construction.

Acid mine drainage (AMD) is a serious and persistent environmental pollution problem. At present, many studies have focused on the tailings pond's cover systems. This paper introduced the research results of using tin tailings from Laili mountain to make the covering layer of tailings pond. The first part included a detailed description of tailings characterization and acid production potential. On this basis, the hard layer (HL) was prepared and its feasibility as oxidation barrier was evaluated. It was found that when the proportion of tailings waste was 70%, the immobilization efficiency of heavy metals can reach more than 99.45%, and the pH of leaching solution was about 10.8. Moreover, the beneficial effect of solid waste addition on the HL was also verified. This suggests that HL as a post-mining restorative strategy has strong positive influence on pollution control.

Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.

The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors. The results showed that 2 mg/L of Fe(III) promoted the biofilm mass and improved the biofilm activity, but 16 mg/L of Fe(III) adversely affected biofilm development. Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm. The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS. This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.

Exposure to O3 and NO2 on the interfacial chemistry of the pulmonary surfactant and the mechanism of lung oxidative damage.

Exposure to ozone (O3) and nitrogen dioxide (NO2) are related to pulmonary dysfunctions and various lung diseases, but the underlying biochemical mechanisms remain uncertain. Herein, the effect of inhalable oxidizing gas pollutants on the pulmonary surfactant (PS, extracted from porcine lungs), a mixture of active lipids and proteins that plays an important role in maintaining normal respiratory mechanics, is investigated in terms of the interfacial chemistry using in-vitro experiments; and the oxidative stress induced by oxidizing gases in the simulated lung fluid (SLF) supplemented with the PS is explored. The results showed that O3 and NO2 individually increased the surface tension of the PS and reduced its foaming ability; this was accompanied by the surface pressure-area isotherms of the PS monolayers shifting toward lower molecular areas, with O3 exhibiting more severe effects than NO2. Moreover, both O3 and NO2 produced reactive oxygen species (ROS) resulting in lipid peroxidation and protein damage to the PS. The formation of superoxide radicals (O2•-) was correlated with the decomposition of O3 and the reactions of O3 and NO2 with antioxidants in the SLF. These radicals, in the presence of antioxidants, led to the formation of hydrogen peroxide and hydroxyl radicals (•OH). Additionally, the direct oxidation of unsaturated lipids by O3 and NO2 further caused an increase in the ROS content. This change in the ROS chemistry and increased •OH production tentatively explain how inhalable oxidizing gases lead to oxidative stress and adverse health effects. In summary, our results indicated that inhaled O3 and NO2 exposure can significantly alter the interfacial properties of the PS, oxidize its active ingredients, and induce ROS formation in the SLF. The results of this study provide a basis for the elucidation of the potential hazards of inhaled oxidizing gas pollutants in the human respiratory system.

Interactions between CuO NPs and PS: The release of copper ions and oxidative damage.

Copper oxide nanoparticles (CuO NPs) can adversely affect lung health possibly by inducing oxidative damage through the release of copper ions. However, the migration and transformation processes of CuO NPs in lung lining fluid is still unclear, and there are still conflicting reports of redox reactions involving copper ions. To address this, we examined the release of copper ions from CuO NPs in simulated lung fluid supplemented with pulmonary surfactant (PS), and further analyzed the mechanisms of PS-CuO NPs interactions and the health hazards. The results showed that the phospholipid of PS was adsorbed on the particle surface, which not only induced aggregation of the particles but also provided a reaction environment for the interaction of PS with CuO NPs. PS was able to promote the release of ions from CuO NPs, of which the protein was a key component. Lipid peroxidation, protein destabilization, and disruption of the interfacial chemistry also occurred in the PS-CuO NPs interactions, during which copper ions were present only as divalent cations. Meanwhile, the contribution of the particle surface cannot be neglected in the oxidative damage to the lung caused by CuO NPs. Through reacting with biomolecules, CuO NPs accomplished ion release and induced oxidative damage associated with PS. This research was the first to reveal the mechanism of CuO NPs releasing copper ions and inducing lipid oxidative damage in the presence of PS, which provides a new idea of transition metal-induced health risk in human body.

The Prognostic and Immune Significance of CILP2 in Pan-Cancer and Its Relationship with the Progression of Pancreatic Cancer.

Cartilage intermediate layer protein 2 (CILP2) facilitates interactions between matrix components in cartilage and has emerged as a potential prognostic biomarker for cancer. This study aimed to investigate the function and mechanisms of CILP2 in pan-cancer. We evaluated the pan-cancer expression, methylation, and mutation data of CILP2 for its clinical prognostic value. Additionally, we explored the immunological characteristics of CILP2 in pan-cancer and then focused specifically on pancreatic ductal adenocarcinoma (PAAD). The subtype analysis of PAAD identified subtype-specific expression and immunological characteristics. Finally, in vitro and in vivo experiments assessed the impact of CILP2 on pancreatic cancer progression. CILP2 exhibited high expression in most malignancies, with significant heterogeneity in epigenetic modifications across multiple cancer types. The abnormal methylation and copy number variations in CILP2 were correlated with poor prognoses. Upregulated CILP2 was associated with TGFB/TGFBR1 and more malignant subtypes. CILP2 exhibited a negative correlation with immune checkpoints in PAAD, suggesting potential for immunotherapy. CILP2 activated the AKT pathway, and it increased proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) in pancreatic cancer. We demonstrated that CILP2 significantly contributes to pancreatic cancer progression. It serves as a prognostic biomarker and a potential target for immunotherapy.

Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.

This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases. The sequencing batch biofilm reactors were developed to cultivate biofilms under different Al(III) dosages. The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm. The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm. Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth. The biofilm could gradually adapt to the inhibition caused by Al(III) at the biofilm maturation moment. Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.

Biotreatment of p-nitrophenol and nitrobenzene in mixed wastewater through selective bioaugmentation.

This work combined selective adsorption and bioaugmentation to treat mixed wastewater of nitrobenzene and p-nitrophenol. The mixed wastewater of nitrobenzene (217 mg/L) and p-nitrophenol (500 mg/L) was adjusted its pH to 8 and then passed through the adsorption column at 100 mL/h. In effluent the nitrobenzene concentration was less than 4 mg/L. Without the toxic inhibition of nitrobenzene, p-nitrophenol in effluent could be degraded within 60 h through bioaugmentation. About 23 mg/g of nitrobenzene adsorbed the dry resin HU-05 could be desorbed and degraded through bioaugmentation. During this process the adsorption capacity of the resin HU-05 was recovered partly. The recovered extent was limited by nitrobenzene bioavailability. The performance of the resin HU-05 kept stably in the recycle experiments of 60 days.

[Biological treatment of nitrobenzene and aniline in interactional inhibitory system through the selective bioaugmentation].

The mixed wastewater containing aniline and nitrobenzene was treated through combining adsorption and bioaugmentation. Tests were conducted to investigate the inhibition of aniline and nitrobenzene on another biodegradation, the condition of adsorption separation, the biodegradation process through bioaugmentation and the variation of resin performance. The experimental results showed that aniline and nitrobenzene interactional inhibited another biodegradation. Adjusting pH to 4 and absorption velocity to 110 mL/h, the mixed wastewater of aniline (330 mg/L) and nitrobenzene (44 mg/L) passed through the adsorption column containing 10 mL resin NDA-150 (7.2 g). In the adsorption effluent the nitrobenzene concentration was not more than 4 mg/L and aniline concentration had no change. The residual aniline in effluent could be degraded through bioaugmentation. During this process about 597 mg nitrobenzene had been adsorbed by resin, in which 224 mg could be desorbed and biodegraded through bioaugmentation. The volumetric loading to nitrobenzene was 315 mg/(L x d). Through bioregeneration the adsorption capacity of resin NDA-150 could be recovered partly, which extent was limited by the bioavailability to nitrobenzene. After about 70 d recycled experiment the performance of resin NDA-150 was stable.