Characterization of Exopolysaccharides Having Potential Antiviral Properties from Priestia Aryabattai Strain MK1 and Bacillus sp. Strain MK2.

Viral diseases are a serious threat to humans while the most antiviral drugs have low efficiency and side effects on human health. Therefore, using microbial biopolymers as the drugs alternate to treat viral infections seems cost-effective and human friendly option. In the present study, thirty-four exopolysaccharides (EPSs) producing bacteria were isolated, and EPSs production capacity of five salt-tolerant isolates was determined under 0, 100 and 150 mM NaCl. Among these, two isolates exhibiting high anti-coliphage activity were identified through 16S rRNA gene analysis. Moreover, the EPSs were characterized by Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis, and their composition was determined. Five salt-tolerant bacteria (MK1, MK2, MK10, MK22 and MK29) exhibited higher production of EPSs at 100 mM NaCl compared to that under non-saline control. At 100 mM NaCl, the yield of EPSs ranged between 105 and 330 mg 100 mL-1 broth. The EPSs produced by the isolates MK1 and MK2 exhibited higher anti-coliphage activity (plaque forming unit decreased from 43 × 106 mL-1 to 3 × 106 and 4 × 106 mL-1, respectively), and were comprised of glucose, fructose, galactose, sucrose, lactose and xylose sugars. FTIR spectroscopy depicted that EPSs are mainly composed of hydroxyl, aliphatic, carboxyl, sulfate and phosphate functional groups, which could have bound coliphage and thus conferred higher anti-coliphage activities to the EPSs. Phylogenetic analysis revealed that MK1 and MK2 isolates formed clades within genus Priestia and Bacillus sequences, respectively. High EPSs production capacity of bacterial isolates under saline condition and high anti-coliphage activity of the EPSs implies that bacterial biopolymers could be useful in antiviral drugs therapy.

Remediation of heavy metal contaminated soils by using Solanum nigrum: A review.

Heavy metals are among the major environmental pollutants and the accumulation of these metals in soils is of great concern in agricultural production due to the toxic effects on crop growth and food quality. Phytoremediation is a promising technique which is being considered as an alternative and low-cost technology for the remediation of metal-contaminated soils. Solanum nigrum is widely studied for the remediation of heavy metal-contaminated soils owing to its ability for metal uptake and tolerance. S. nigrum can tolerate excess amount of certain metals through different mechanism including enhancing the activities of antioxidant enzymes and metal deposition in non-active parts of the plant. An overview of heavy metal uptake and tolerance in S. nigrum is given. Both endophytic and soil microorganisms can play a role in enhancing metal tolerance in S. nigrum. Additionally, optimization of soil management practices and exogenous application of amendments can also be used to enhance metal uptake and tolerance in this plant. The main objective of the present review is to highlight and discuss the recent progresses in using S. nigrum for remediation of metal contaminated soils.

Citric acid assisted phytoremediation of cadmium by Brassica napus L.

Phytoextraction is an eco-friendly and cost-effective technique for removal of toxins, especially heavy metals and metalloids from contaminated soils by the roots of high biomass producing plant species with subsequent transport to aerial parts. Lower metal bioavailability often limits the phytoextraction. Organic chelators can help to improve this biological technique by increasing metal solubility. The aim of the present study was to investigate the possibility of improving the phytoextraction of Cd by the application of citric acid. For this purpose, plants were grown in hydroponics under controlled conditions. Results indicated that Cd supply significantly decreased the plant growth, biomass, pigments, photosynthetic characteristics and protein contents which were accompanied by a significant increase in Cd concentration, hydrogen peroxide (H2O2), electrolyte leakage, malondialdehyde (MDA) accumulation and decrease in antioxidant capacity. The effects were dose dependent with obvious effects at higher Cd concentration. Application of CA significantly enhanced Cd uptake and its accumulation in plant roots, stems and leaves. Citric acid alleviated Cd toxicity by increasing plant biomass and photosynthetic and growth parameters alone and in combination with Cd and by reducing oxidative stress as observed by reduction in MDA and H2O2 production and decreased electrolyte leakage induced by Cd stress. Application of CA also enhanced the antioxidant enzymes activity alone and under Cd stress. Thus, the data indicate that exogenous CA application can increase Cd uptake and minimize Cd stress in plants and may be beneficial in accelerating the phytoextraction of Cd through hyper-accumulating plants such as Brassica napus L.

Alleviation of cadmium toxicity by silicon is related to elevated photosynthesis, antioxidant enzymes; suppressed cadmium uptake and oxidative stress in cotton.

Biotic systems face immense environmental hazards such as accumulation of heavy metals, particularly in agricultural ecosystems that might cause deterioration of yield and quality of crops. In this study, we evaluated the role of silicon (Si) in alleviating the heavy metal (Cd) stress tolerance in cotton by analyzing the induced Physio-chemical changes. Cotton plants were grown in hydroponic culture with three different Cd levels (0, 1 and 5µM) along with two Si treatment levels (0 and 1mM). The data showed that Cd alone reduced the plant growth as well as the efficiency of antioxidant activity as compared to control plants. Plant growth, gas exchange characteristics (net photosynthetic rate, stomatal conductance, transpiration rate, water use efficiency) chlorophyll contents, and carotenoids as well as the performance of antioxidant enzymes were improved by the exogenous application of Si. The adverse effects of Cd on plant growth were alleviated by the exogenous application of Si. It was observed that Si effectively mitigated the adverse effects of Cd on cotton plants and markedly enhanced the growth, biomass and photosynthetic parameters while decreased the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and electrolytic leakage (EL). The antioxidant enzyme activities in cotton leaves and roots increased significantly, when Si was added to control as well as Cd stressed plants. In conclusion, Si improved the growth and photosynthesis attributes of cotton plants by mitigating the adverse effects of Cd stress through reduced EL, MDA and H2O2 contents and improved activities of antioxidant enzymes.

Exploring the Impacts of Genotype-Management-Environment Interactions on Wheat Productivity, Water Use Efficiency, and Nitrogen Use Efficiency under Rainfed Conditions.

Wheat production under rainfed conditions is restrained by water scarcity, elevated temperatures, and lower nutrient uptake due to possible drought. The complex genotype, management, and environment (G × M × E) interactions can obstruct the selection of suitable high yielding wheat cultivars and nitrogen (N) management practices prerequisite to ensure food security and environmental sustainability in arid regions. The agronomic traits, water use efficiency (WUE), and N use efficiencies were evaluated under favorable and unfavorable weather conditions to explore the impacts of G × M × E on wheat growth and productivity. The multi-N rate (0, 70, 140, 210, and 280 kg N ha-1) field experiment was conducted under two weather conditions (favorable and unfavorable) using three wheat cultivars (AUR-809, CHK-50, and FSD-2008) in the Pothowar region of Pakistan. The experiments were laid out in randomized complete block design (RCBD), with split plot arrangements having cultivars in the main plot and N levels in the subplot. The results revealed a significant decrease in aboveground biomass, grain yield, crop N-uptake, WUE, and N use efficiency (NUE) by 15%, 22%, 21%, 18%, and 8%, respectively in the unfavorable growing season (2014-2015) as compared to favorable growing season (2013-2014) as a consequence of less rainfall and heat stress during the vegetative and reproductive growth phases, respectively. FSD-2008 showed a significantly higher aboveground biomass, grain yield, crop N-uptake, WUE, and NUE as compared to other wheat cultivars in both years. Besides, N140 appeared as the most suitable dose for wheat cultivars during the favorable growing season. However, any further increase in N application rates beyond N140 showed a non-significant effect on yield and yield components. Conversely, the wheat yield increased significantly up to 74% from N0 to N70 during the unfavorable growing season, and there was no substantial difference between N70-N280. The findings provide opportunities for maximizing yield while avoiding excessive N loss by selecting suitable cultivars and N application rates for rainfed areas of Pothowar Plateau by using meteorological forecasting, amount of summer rainfall, and initial soil moisture content.

Relief Role of Lysine Chelated Zinc (Zn) on 6-Week-Old Maize Plants under Tannery Wastewater Irrigation Stress.

Tannery wastewater mainly comes from leather industries. It has high organic load, high salinity, and many other pollutants, including chromium (Cr). Tannery wastewater is generally used for crop irrigation in some areas of Pakistan and worldwide, due to the low availability of good quality of irrigation water. As tannery wastewater has many nutrients in it, its lower concentration benefits the plant growth, but at a higher concentration, it damages the plants. Chromium in tannery wastewater accumulates in plants, and causes stress at physiological and biochemical levels. In recent times, the role of micronutrient-amino acid chelated compounds has been found to be helpful in reducing abiotic stress in plants. In our present study, we used lysine chelated zinc (Zn-lys) as foliar application on maize (Zea mays L.), growing in different concentrations of tannery wastewater. Zinc (Zn) is required by plants for growth, and lysine is an essential amino acid. Maize plants were grown in tannery wastewater in four concentrations (0, 25%, 50%, and 100%) and Zn-lys was applied as a foliar spray in three concentrations (0 mM, 12.5 mM, and 25 mM) during plant growth. Plants were cautiously harvested right after 6 weeks of treatment. Foliar spray of Zn-lys on maize increased the biomass and improved the plant growth. Photosynthetic pigments such as total chlorophyll, chlorophyll a, chlorophyll b and contents of carotenoids also increased with Zn-lys application. In contrast to control plants, the hydrogen peroxide (H2O2) contents were increased up to 12%, 50%, and 68% in leaves, as well as 16%, 51% and 89% in roots at 25%, 50%, and 100% tannery water application, respectively, without Zn-lys treatments. Zn-lys significantly reduced the damages caused by oxidative stress in maize plant by decreasing the overproduction of H2O2 and malondialdehyde (MDA) in maize that were produced, due to the application of high amount of tannery wastewater alone. The total free amino acids and soluble protein decreased by 10%, 31% and 64% and 18%, 61% and 122% at 25%, 50% and 100% tannery water treatment. Zn-lys application increased the amino acids production and antioxidant activities in maize plants. Zn contents increased, and Cr contents decreased, in different parts of plants with Zn-lys application. Overall, a high concentration of tannery wastewater adversely affected the plant growth, but the supplementation of Zn-lys assertively affected the plant growth and enhanced the nutritional quality, by enhancing Zn and decreasing Cr levels in plants simultaneously irrigated with tannery wastewater.

Effect of foliar-applied iron complexed with lysine on growth and cadmium (Cd) uptake in rice under Cd stress.

Contamination of soils with cadmium (Cd) is a serious problem worldwide. Rice (Oryza sativa L.) is reported to accumulate relatively higher Cd contents in consumable parts and is considered a main source of Cd toxicity to humans from rice-derived products. The aim of this pot trial was to investigate the effect of foliar-applied iron (Fe) complexed with lysine on growth, photosynthesis, Cd concentration in plants, oxidative stress, and activities of antioxidants of rice in soil contaminated with Cd. Rice seedlings (30-day-old) were transferred to the soil, and after 2 weeks, different concentrations of Fe-lysine (0, 1.5, 3.0, 4.5, 6.0, and 7.5 mg L-1) were applied as a foliar spray once in a week for 4 weeks and plant samples were taken after 10 weeks of growth in the soil under ambient conditions. Foliar supply of Fe-lysine complex significantly enhanced the plant height, dry weights of plants, concentration of chlorophyll, and gas exchange attributes in Cd-stressed rice. Fe-lysine decreased the Cd concentrations in plants while increasing the Fe concentrations in rice seedlings being maximum with Fe-lysine of 6.0 mg L-1. Electrolyte leakage decreased while activities of key antioxidant enzymes increased with Fe-lysine compared to the control. According to the present results, Fe-lysine complex can effectively be used to reduce Cd concentrations in rice and probably in other crop species.

Management of tannery wastewater for improving growth attributes and reducing chromium uptake in spinach through citric acid application.

The use of chromium (Cr)-contaminated tannery wastewater for irrigation is a common practice, especially in developing countries like Pakistan. This practice is due to the shortage of good quality irrigation water for crop growth as well as the issue of tannery wastewater disposal. The current study was done to evaluate the effect of citric acid (CA) (0, 1.0, and 2.0 mM) on the growth and Cr uptake by spinach irrigated with different mixtures of tap water and tannery wastewater (100:0, 50:50, and 0:100 tap water to wastewater ratio). Plants were grown for 8 weeks under ambient conditions. Results showed that 50:50% tap water and wastewater increased plant height, dry weights of shoots and roots, total chlorophyll contents, and gas exchange attributes than the plants treated with only tap water or only wastewater. Increasing wastewater ratio increased electrolyte leakage (EL) in plants and enhanced the leaf key antioxidant enzyme activities as well as increased Cr contents. Foliar application of CA increased the plant dry weights, photosynthesis, and enzyme activities, whereas reduced the EL and Cr concentrations in plants than respective treatments without CA application. It can be concluded that 50:50 tap water and wastewater irrigation along with foliar CA application might be an effective strategy for increasing vegetable growth with reduced metal concentrations.

Foliar application of aspartic acid lowers cadmium uptake and Cd-induced oxidative stress in rice under Cd stress.

Cadmium (Cd) contamination of farmland soils is a widespread problem around the globe, and rice (Oryza sativa L.) tends to accumulate more Cd and is considered as one of the major sources of Cd intake in humans, especially consuming rice-derived products. The current study investigated the effects of foliar applied aspartic acid (Asp) on growth parameters, biomass, chlorophyll concentration, gas exchange characteristics, Cd uptake, and antioxidative capacity in the shoots and roots of rice seedlings exposed to Cd stress. For this, 30-day-old rice nursery was transferred in the soil with aged Cd contamination (2.86 mg kg-1). After 2 weeks of growth, different concentrations (0, 10, 15, and 20 mg L-1) of Asp were foliar applied four times with a 7-day interval, and the crop was harvested after 10 weeks of transplanting. Foliar applied Asp increased the plant height, shoot and root dry weight, chlorophyll concentration, and gas exchange parameters, while it reduced the Cd concentrations in both shoots and roots as well as shoot to root Cd translocation factor compared to the control. Foliar application of Asp reduced the malondialdehyde content and electrolyte leakage in rice parts compared to the control in a dose-additive manner. The activities of key antioxidant enzymes increased while peroxidase activity decreased by exogenous Asp. The increase in plant weight and photosynthesis might be due to lower Cd concentrations in plants which may reduce the oxidative stress and also help the plants to minimize direct damage caused by Cd to the photosynthetic organs.

Phytoremediation strategies for soils contaminated with heavy metals: Modifications and future perspectives.

Presence of heavy metals in agricultural soils is of major environmental concern and a great threat to life on the earth. A number of human health risks are associated with heavy metals regarding their entry into food chain. Various physical, chemical and biological techniques are being used to remove heavy metals and metalloids from soils. Among them, phytoremediation is a good strategy to harvest heavy metals from soils and have been proven as an effective and economical technique. In present review, we discussed various sources and harmful effects of some important heavy metals and metalloids, traditional phytoremediation strategies, mechanisms involved in phytoremediation of these metals, limitations and some recent advances in phytoremediation approaches. Since traditional phytoremediation approach poses some limitations regarding their applications at large scale, so there is a dire need to modify this strategy using modern chemical, biological and genetic engineering tools. In view of above, the present manuscript brings both traditional and advanced phytoremediation techniques together in order to compare, understand and apply these strategies effectively to exclude heavy metals from soil keeping in view the economics and effectiveness of phytoremediation strategies.

Silicon (Si) alleviates cotton (Gossypium hirsutum L.) from zinc (Zn) toxicity stress by limiting Zn uptake and oxidative damage.

Silicon (Si) is as an important fertilizer element, which has been found effective in enhancing plant tolerance to variety of biotic and a-biotic stresses. This study investigates the Si potential to alleviate zinc (Zn) toxicity stress in cotton (Gossypium hirsutum L.). Cotton plants were grown in hydroponics and exposed to different Zn concentration, 0, 25, and 50 µM, alone and/or in combination with 1 mM Si. Incremental Zn concentration in growth media instigated the cellular oxidative damage that was evident from elevated levels of hydrogen peroxide (H2O2), electrolyte leakage, and malondialdehyde (MDA) and consequently inhibited cotton growth, biomass, chlorophyll pigments, and photosynthetic process. Application of Si significantly suppressed Zn accumulation in various plant parts, i.e., roots, stems, and leaves and thus promoted biomass, photosynthetic, growth parameters, and antioxidant enzymes activity of Zn-stressed as well unstressed plants. In addition, Si reduced the MDA and H2O2 production and electrolyte leakage suggesting its role in protecting cotton plants from Zn toxicity-induced oxidative damage. Thus, the study indicated that exogenous Si application could improve growth and development of cotton crop experiencing Zn toxicity stress by limiting Zn bioavailability and oxidative damage.