RESUMEN
PURPOSE: To assess the efficacy and safety of various intraocular lenses (IOLs), including standard monofocal, bifocal, trifocal, extended depth of focus (EDOF), and enhanced monofocal IOLs, post-cataract surgery through a network meta-analysis. METHODS: A systematic search of PubMed, Cochrane Library, and Web of Science was conducted to identify relevant studies from the past 5 years. Parameters such as binocular visual acuities, spectacle independence, contrast sensitivity (CS), and optical quality were used to evaluate efficacy and safety. Data from the selected studies were analyzed using Review Manager 5.4 and STATA 17.0 software. RESULTS: Twenty-eight Randomized Controlled Trials (RCTs) comprising 2465 subjects were included. Trifocal IOLs exhibited superior uncorrected near visual acuity (UNVA) compared to monofocal IOLs (MD: -0.35; 95% CI: -0.48, -0.22). Both trifocal (AcrySof IQ PanOptix IOLs group MD: -0.13; 95% CI: -0.21, -0.06) and EDOF IOLs (MD: -0.13; 95% CI: -0.17, -0.09) showed better uncorrected intermediate visual acuity (UIVA) than monofocal IOLs. Trifocal IOLs ranked highest in spectacle independence at various distances (AT LISAtri 839MP group: SUCRA 97.5% for distance, 80.7% for intermediate; AcrySof IQ PanOptix group: SUCRA 83.0% for near). CONCLUSIONS: For cataract patients who want to treat presbyopia, trifocal IOLs demonstrated better visual acuity and spectacle independence at near distances. Different types of trifocal IOL characteristics differ. EDOF and enhanced monofocal IOLs have improved visual quality at intermediate distances.Therefore, It is very important to select the appropriate IOLs based on the lens characteristics and patient needs.
Asunto(s)
Extracción de Catarata , Lentes Intraoculares , Presbiopía , Agudeza Visual , Humanos , Presbiopía/cirugía , Presbiopía/fisiopatología , Agudeza Visual/fisiología , Extracción de Catarata/métodos , Sensibilidad de Contraste/fisiología , Implantación de Lentes Intraoculares/métodos , Diseño de Prótesis , Visión Binocular/fisiología , Refracción Ocular/fisiologíaRESUMEN
BACKGROUND: Diabetic retinopathy (DR) is a common complication of diabetes mellitus, which is a major reason of blindness. Baicalin (BAI) is a flavonoid extracted from Scutellaria baicalensis, whose pharmacological characterizes have been widely reported in various diseases. However, it remains unclear the effect of BAI on DR. The study aimed to confirm the protective effect of BAI on DR. METHODS: ARPE-19 cells and HRMECs were exposed to the high glucose (HG) environment to construct a cell injury model. After treatment with HG and BAI, cell viability, apoptosis, inflammatory cytokines and ROS generations were determined in ARPE-19 cells and HRMECs. Subsequently, microRNA-145 (miR-145) inhibitor and its negative control were transfected into ARPE-19 cells, and the regulatory effects on HG-and BAI-co-treated cells were detected. NF-κB and p38MAPK signaling pathways were finally examined to state the underling mechanisms. RESULTS: HG treatment significantly induced ARPE-19 cells and HRMECs injury in vitro. BAI significantly promoted cell proliferation, reduced apoptosis, as well as inhibited the release of IL-1ß, IL-6, IL-8 and ROS level in HG-injured ARPE-19 cells and HRMECs. Additionally, the expression level of miR-145 was up-regulated in HG-and BAI-co-treated cells. More importantly, miR-145 inhibition reversed the protective effect of BAI on HG-injured ARPE-19 cells. Besides, we observed that BAI inhibited the activations of NF-κB and p38MAPK pathways by up-regulating miR-145. CONCLUSIONS: Results demonstrated that BAI exhibited the protective effect against HG-induced cell injury by up-regulation of miR-145.
Asunto(s)
Flavonoides/farmacología , Glucosa/toxicidad , MicroARNs/biosíntesis , Epitelio Pigmentado de la Retina/efectos de los fármacos , Apoptosis/efectos de los fármacos , Línea Celular , Retinopatía Diabética/tratamiento farmacológico , Evaluación Preclínica de Medicamentos , Humanos , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , MicroARNs/antagonistas & inhibidores , MicroARNs/genética , FN-kappa B/antagonistas & inhibidores , FN-kappa B/metabolismo , Especies Reactivas de Oxígeno , Epitelio Pigmentado de la Retina/citología , Regulación hacia Arriba/efectos de los fármacos , Proteínas Quinasas p38 Activadas por Mitógenos/antagonistas & inhibidores , Proteínas Quinasas p38 Activadas por Mitógenos/fisiologíaRESUMEN
Background: Retinoblastoma (RB) is the commonest primary intraocular malignancy during childhood. Circular RNAs (circRNAs) act as regulators in RB development, and hsa_circ_E2F5 (circ_0084811 in this study) was found to be highly expressed in RB cells, so we wanted to identify its detailed molecular mechanism. Methods: The expression level of circ_0084811 in RB cells was tested by RT-qPCR and its effects on RB cells were evaluated through functional assays. The regulatory mechanism that circ_0084811 may exert in RB progression was testified through mechanism experiments. Results: High circ_0084811 expression in RB cells facilitated cell proliferation but inhibited cell apoptosis. The enrichment of acetylation of histone 3 lysine 27 (H3K27ac) in circ_0084811 promoter induced circ_0084811 upregulation. Moreover, circ_0084811 regulated E2F transcription factor 5 (E2F5) expression via sponging microRNA-18a-5p (miR-18a-5p) and microRNA-18b-5p (miR-18b-5p). Conclusion: circ_0084811 modulated RB progression via the miR-18a-5p/miR-18b-5p/E2F5 axis.
Asunto(s)
MicroARNs , Neoplasias de la Retina , Retinoblastoma , Apoptosis/genética , Línea Celular Tumoral , Movimiento Celular/fisiología , Proliferación Celular/genética , Factor de Transcripción E2F5 , Humanos , MicroARNs/genética , MicroARNs/metabolismo , Neoplasias de la Retina/genética , Retinoblastoma/genéticaRESUMEN
A new lycopodane-type Lycopodium alkaloid, 6α-hydroxy-5,15-oxide-lycopodane (1), and seven known alkaloids were isolated from the whole plants of Huperzia serrata. Their structures were elucidated by means of spectroscopic methods. 12-Deoxyhuperzine O (2) was reported as a naturally occurring alkaloid for the first time, and showed an antagonist effect on the N-methyl-d-aspartate receptor with an IC(50) value of 0.92 µM.
Asunto(s)
Alcaloides/aislamiento & purificación , Medicamentos Herbarios Chinos/aislamiento & purificación , Huperzia/química , Alcaloides/química , Alcaloides/farmacología , Animales , Corteza Cerebral/efectos de los fármacos , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/farmacología , Concentración 50 Inhibidora , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Ratas , Ratas Sprague-Dawley , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Membranas Sinápticas/efectos de los fármacosRESUMEN
In order to further understand the interaction processes between the Lycopodium alkaloids and acetylcholinesterase, the binding properties of N-acetyl huperzine A (1), huperzine B (2) and huperzine F (3) with Torpediniforms Nacline acetylcholinesterase (TnAchE) were investigated by 1H NMR methods. The nonselective, selective and double-selective spin-lattice relaxation rates were acquired in the absence and presence of TnAchE at a ratio of [ligand]/[protein]=1:0.005. The selective relaxation rates show protons of 1-3 have dipole-dipole interaction with protons of TnAchE at the binding interface. The molecular rotational correlation time of bound ligands was calculated by double-selective relaxation rate at 298 K, which showed that 1-3 had high affinity with the protein. The results indicate that investigation of 1H NMR relaxation data is a useful method to locate the new Lycopodium alkaloids as AchE inhibitors.
Asunto(s)
Acetilcolinesterasa/química , Alcaloides/química , Inhibidores de la Colinesterasa/química , Lycopodium/química , Hidrógeno/química , Resonancia Magnética Nuclear Biomolecular , Quinolizinas/química , Sesquiterpenos/químicaRESUMEN
AIM: To summarize preoperative evaluation and outcome of corneal transplantation for limbal dermoids for ten years. METHODS: Eighty-five patients diagnosed with limbal dermoids and treated with corneal transplantation were analyzed retrospectively. All patients were further divided into two groups according to absence or presence of neovascularization surrounding the dermoids in the corneal stroma. Eighty-two eyes were treated with tumor excision combined with partial lamellar sclerokeratoplasty, and the other three eyes were performed by penetrating keratoplasty. The size and location of the tumor, the associated ocular and systemic anomalies, the depth of the corneal penetration of tumor tissues, the preoperative and postoperative best-corrected visual acuity (BCVA), graft survival and cosmetic outcome, and surgical complications were recorded respectively. RESULTS: The average age at surgery was 5.3y (range, 3mo-36y). The mean size of dermoids was 6.1±1.6 mm. The 43.5% of eyes (37/85) were present with hair at the surface of the dermoid and 72.9% of dermoids were located inferotemporal of the eye. Amplyopia was present in 34.1% of patients (29/85) and 9.4% of patients (8/85) had lipodermoids. Eighteen patients suffered from Goldenhar's syndrome with an accessory ear. The 75% of patients in group 1 had involvement of the corneal deep stroma down to Descemet's membrane without involving it, but 71.4% of patients had Descemet's membrane involvement in group 2. Preoperative BCVA ranged from counting fingers to 20/20. Postoperatively 81.1% had a BCVA of 20/800 or better. There was no significant difference between the post-surgical BCVA of the two groups (t=1.584, P>0.05). The grafts of 70.5% patients were present as 1+ opacity, 21.1% as 2+ opacity, 8.2% as 3+ opacity and none as 4+ opacity. Surgical complications included graft rejection, microperforation, prolonged reepithelialization, steroid glaucoma, interface neovascularization, and interface hemorrhage. CONCLUSION: The dermoids with neovascularization surrounding them in the corneal stroma invaded deeper tissues in the cornea than those with no neovascularization surrounding them in the corneal stroma. Therefore, surgeons should take care to avoid corneal perforation during the corneal transplantation operation. The majority of patients markedly improved their cosmetic appearance after surgery.
RESUMEN
Sixteen serratane-type triterpenoids including three new compounds, 14beta,15beta-epoxyserratan-3beta,21beta,29-triol (1), serrat-14-en-3beta,21beta,29-triol (2) and serrat-14-en-3alpha,21beta,24,29-tetraol (3), were isolated from the whole plant of Huperzia serrata (Thunb) Trev. The structures of these new compounds (1-3) were elucidated on the basis of spectral analysis.
Asunto(s)
Huperzia , Fitoterapia , Triterpenos/química , Humanos , Espectroscopía de Resonancia Magnética , Componentes Aéreos de las Plantas , Raíces de PlantasRESUMEN
OBJECTIVE: To reveal the pharmacological activities of the components for their further utilization and development by studying the chemical constituents of Citrus changshan-huyou. METHOD: The structures were determined by repeated silica gel chromatographic separation and spectral analysis. RESULT: Five compounds were obtained, and identified as 3-oxo friedelin (I), limonin (II), beta-sitosterol (III), 8-(2',3'-dihydroxy-4'-methylbutane)-7-methoxycoumarin (IV), sucrose (V). CONCLUSION: The five compounds were obtained from this plant for the first time.
Asunto(s)
Citrus/química , Limoninas/aislamiento & purificación , Plantas Medicinales/química , Sitoesteroles/aislamiento & purificación , Frutas/química , Limoninas/química , Sitoesteroles/química , Sacarosa/química , Sacarosa/aislamiento & purificaciónRESUMEN
Two new Lycopodium alkaloids, N-oxidehuperzine E (1) and N-oxidehuperzine F (2), along with two known alkaloids, huperzines E (3) and F (4), were isolated from Huperzia serrata (Thunb.) Trev. Their structures were elucidated by spectroscopic and chemical transformations.
Asunto(s)
Alcaloides/química , Huperzia/química , Extractos Vegetales/química , Estructura Molecular , Resonancia Magnética Nuclear BiomolecularRESUMEN
Callianthones A ( 1) and B ( 2), a pair of new isomeric alpha-tetralones, together with a known alpha-tetralone ( 3) and four known flavonoids ( 4 - 7) were isolated from the 50 % EtOH extract of Pyrola calliantha. The structures and absolute configurations of the two new isomers were established to be (2 S,4 R) - and (2 S,4 S)-2,4-dihydroxy-2,7-dimethyl-3,4-dihydronaphthalen-1(2 H)-one ( 1 and 2, respectively) on the basis of spectral analysis, including 2 D NMR, model studies, and CD spectra.
Asunto(s)
Extractos Vegetales/aislamiento & purificación , Pyrola/química , Tetralonas/aislamiento & purificación , Isomerismo , Modelos Estructurales , Estructura Molecular , Extractos Vegetales/química , Tetralonas/químicaRESUMEN
Three new indole N-oxide alkaloids, fargesine (5-hydroxy-12-methyl-10,11,12,13-tetrahydro-1 H-azepino[5,4,3-cd]indole N(12)-oxide, 1), plectocomine 12-methyl-5- O-beta-D-glucopyranoside N(12)-oxide (2), and bufotenine 5-O-beta-D-glucopyranoside N(12)-oxide (3), were isolated from the root and stem of Evodia fargesii Dode along with five known compounds. The structures of the new alkaloids were elucidated on the basis of spectroscopic analysis.
Asunto(s)
Evodia , Fitoterapia , Extractos Vegetales/química , Alcaloides/química , Humanos , Indoles/química , Espectroscopía de Resonancia Magnética , Raíces de Plantas , Tallos de la PlantaRESUMEN
Four new eremophilanlides (1- 4) together with a known sesquiterpene were isolated from the roots and rhizomes of Ligularia hodgsonii. The structures of these new compounds were established as 6beta-(2'-methylbutanoyloxy)-10beta-acetoxy-3 beta, 8beta-dihydroxyeremophil-7(11)-en-8,12-olide (1), 6beta,10 beta-dihydroxyeremophil-7(11)-en-8alpha,12-olide (2), 3beta-acetoxy-6,9-dien-8-oxoeremophil-12-nor-11-ketone (3), and 3beta-acetoxy-6alpha, 8alpha-dihydroxyeremophil-7(11), 9-dien-8,12-olide (4) by means of mass, IR and NMR spectroscopy.
Asunto(s)
Asteraceae , Fitoterapia , Extractos Vegetales/química , Humanos , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Naftalenos/química , Raíces de Plantas , Sesquiterpenos Policíclicos , Rizoma , Sesquiterpenos/química , Espectrofotometría InfrarrojaRESUMEN
Two new steroid saponins, named terrestrinins A (1) and B (2), along with six known compounds were isolated from the Chinese medicine herb Tribulus terrestris, and their chemical structures were elucidated as 26-O-beta-D-glucopyranosyl-(25S)-furostan-4(5),20(22)-diene-3,12-dione (1) and 26-O-beta-D-glucopyranosyl-(25S)-5alpha-furostane-3beta,22alpha,26-triol-3-O-beta-D-xylopyranosyl(1 --> 3)-[(beta-D-xylopyranosyl(1 --> 2)]-beta-D-glucopyranosyl(1 --> 4)-[alpha-L-rhamnopyranosyl(1 --> 2)]-beta-D-galactopyranoside (2) on the basis of spectroscopic techniques.
Asunto(s)
Medicamentos Herbarios Chinos/química , Fitoterapia , Saponinas/química , Tribulus , Frutas , HumanosRESUMEN
Seven new and 15 known serratene-type triterpenoids were isolated from the whole plant of Huperzia serrata. The structures of these new triterpenoids were elucidated as 21alpha-hydroxyserrat-14-en-3beta-yl p-dihydrocoumarate (1), 21alpha-hydroxyserrat-14-en-3beta-yl dihydrocaffeate (2), 21alpha-hydroxyserrat-14-en-3beta-yl propanedioic acid monoester (3), 3alpha,21alpha-dihydroxyserrat-14-en-24-oic acid (4), 16-oxo-3alpha,21beta-dihydroxyserrat-14-en-24-al (5), 16-oxo-3alpha,21beta-dihydroxyserrat-14-en-24-oic acid (6), and 16-oxo-21beta-hydroxyserrat-14-en-3alpha-yl acetate (7), respectively, by means of spectroscopic analysis.
Asunto(s)
Medicamentos Herbarios Chinos/aislamiento & purificación , Huperzia/química , Plantas Medicinales/química , Triterpenos/química , Triterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , EstereoisomerismoRESUMEN
The binding properties of huperzine A (1) with Torpediniforms Nacline acetylcholinesterase (TnAChE) were investigated by (1)H NMR methods. The noselective, selective and double-selective spin-lattice relaxation rates were acquired in absent and present of TnAChE at a ratio [ligand]/[protein]=1:0.005. The selective relaxation rates shown protons of 1 had dipole-dipole interaction with protein active site protons. The motional correlation time of bound ligand was calculated by double-selective relaxation rate at 1 tau(2,3)=40.5 ns at 298 K, which showed 1 had high affinity with TnAChE. The experiments give a possible method to use TnAChE to locate the new huperzine A derivatives as AChE inhibitors.
Asunto(s)
Inhibidores de la Colinesterasa/aislamiento & purificación , Huperzia , Sesquiterpenos/aislamiento & purificación , Alcaloides , Animales , Inhibidores de la Colinesterasa/farmacología , Hidrógeno , Espectroscopía de Resonancia Magnética/métodos , Sesquiterpenos/química , Sesquiterpenos/farmacología , TorpedoRESUMEN
A known compound, serratidine (1), along with three hydroxylated serratidine alkaloids, 6alpha-hydroxyserrati dine (2), 4alpha-hydroxyserratidine (3) and 4alpha,6alpha-dihydroxyserratidine (4) were isolated from the CHCl3 fraction of basic materials of whole plant of the Chinese medicinal herb Huperzia serrata. The relative configurations of the above compounds were determined based on 2D NMR studies.
Asunto(s)
Alcaloides/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Plantas Medicinales/química , Alcaloides/química , Cromatografía en Capa Delgada , Medicina Tradicional China , Conformación Molecular , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Two new norditerpenoid alkaloids, 8-acetyl-14-p-methoxybenzoate of talatisamine (1) and 14-p-methoxybenzoate of talatisamine (2), were isolated from Aconitum bulleyanum Diels. The structures were elucidated on the basis of spectroscopic and chemical studies.
Asunto(s)
Aconitina/análogos & derivados , Aconitum , Alcaloides/química , Diterpenos/química , Extractos Vegetales/química , Aconitina/química , Aconitina/aislamiento & purificación , Alcaloides/aislamiento & purificación , Diterpenos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Estructura Molecular , Extractos Vegetales/aislamiento & purificaciónRESUMEN
Three new serratane-type epoxytriterpenoids were isolated from the whole plant of Huperzia serrata. The structures of these new compounds were characterized as 14 beta,15 beta-epoxy-3 beta-hydroxyserratan-21 beta-ol (1), 14 beta,15 beta-epoxy-3 beta-hydroxyserratan-21alpha-ol (2), and 14 beta,15 beta-epoxy-3 beta-hydroxyserratan-21alpha-ol-3 beta-O-acetate (3), respectively, on the basis of spectral data and for 1 by single-crystal X-ray analysis.
Asunto(s)
Huperzia/química , Triterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Triterpenos/químicaRESUMEN
Three new lycopodium alkaloids, 11alpha-hydroxyphlegmariurine B (2), 7alpha-hydroxyphlegmariurine B (3) and 7alpha,11alpha-dihydroxyphlegmariurine B (4) along with a known compound, phlegmariurine B (1), were isolated from the herb Huperzia serrata. The structures of the above compounds were elucidated based on spectroscopic studies.