RESUMEN
Paeonia emodi is one of the ethno therapeutically important Himalayan plants used to cure various diseases. However, a systematic investigation of the effect of altitude on phytochemical, antioxidant, and antimicrobial activity has not been reported so far. The present study assessed the variation in the bioactive compounds, antioxidant and antimicrobial activity of the leaf, and rhizome of P. emodi collected from different altitudes. Phytochemicals such as phenols, flavonoids, flavanol, tannins, emodin, and paeoniflorin were found in all the sampled populations, but the quantity varied significantly across the altitude. In leaf, phenolics, flavonoids, and tannins content positively correlated with altitude (p < 0.01), but flavanol did not show any connection. Similarly, in the rhizome, positive relation with altitude (p < 0.01) was observed in phenol, flavonoids, and paeoniflorin. Antioxidant activity measured by 1, 1-diphenyl- 2 picrylhydrazyl (DPPH) and nitric oxide assays showed a positive correlation (p < 0.05) with altitude. 2, 2-azinobis (3-ethylbenzothiazoline-6-sulphonic acid), ferric reducing antioxidant power, and hydroxyl ion assays did not show any relation with altitude. Antimicrobial activity was higher in the case of rhizome for the minimum inhibitory concentration and positively correlated with phenolics, flavonoids, and flavanol (p < 0.05). The present study further revealed that the secondary metabolites in the leaf and rhizome extracts of P. emodi are an excellent source of antioxidant and antimicrobial activity, thus validating the species' therapeutic potential. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-022-01242-z.
RESUMEN
Medicinal plants are global sources of herbal products, drugs, and cosmetics. They are disappearing rapidly due to anthropogenic pressure, overexploitation, unsustainable harvesting, lack of knowledge on cultivation, and the availability of quality plating materials. In this context, standardized in-vitro propagation protocol was followed to produce Valeriana jatamansi Jones, and transferred in two locations at Kosi-Katarmal (GBP) Almora (1200 masl) and Sri Narayan Ashram (SNA) Pithoragarh (Altitude 2750 masl), Uttarakhand. Over the three years of growth, plants were gathered from both locations for determining biochemical and physiological parameters, and growth performance. The plants growing at Sri Narayan Ashram (SNA) showed considerably (p < 0.05) higher amounts of polyphenolics, antioxidant activities, and phenolic compounds. Similarly, physiological parameters (transpiration 0.004 mol m-2 s-1; photosynthesis 8.20 µmol m-2 s-1; stomatal conductance 0.24 mol m-2 s-1), plant growth performance (leaves number 40, roots number 30, root length 14 cm) and soil attributes (total nitrogen 9.30; potassium 0.025; phosphorus 0.34 mg/g, respectively) were found best in the SNA as compared to GBP. In addition, moderate polar solvent (i.e., acetonitrile and methanol) was found suitable for extracting higher bioactive constituents from plants. The findings from this study revealed that large-scale cultivation of V. jatamansi should promote at higher elevation areas such as Sri Narayan Ashram to harness the maximum potential of the species. Such a protective approach with the right interventions will be helpful to provide livelihood security to the local populace along with quality material for commercial cultivation. This can fulfill the demand through regular supply of raw material to the industries and simultaneously promote their conservation.
Asunto(s)
Valeriana , Fitoquímicos/química , Valeriana/anatomía & histología , Valeriana/química , Altitud , FilogeniaRESUMEN
Plant diseases are still the main problem for the reduction in crop yield and a threat to global food security. Additionally, excessive usage of chemical inputs such as pesticides and fungicides to control plant diseases have created another serious problem for human and environmental health. In view of this, the application of plant growth-promoting rhizobacteria (PGPR) for controlling plant disease incidences has been identified as an eco-friendly approach for coping with the food security issue. In this review, we have identified different ways by which PGPRs are capable of reducing phytopathogenic infestations and enhancing crop yield. PGPR suppresses plant diseases, both directly and indirectly, mediated by microbial metabolites and signaling components. Microbial synthesized anti-pathogenic metabolites such as siderophores, antibiotics, lytic enzymes, hydrogen cyanide, and several others act directly on phytopathogens. The indirect mechanisms of reducing plant disease infestation are caused by the stimulation of plant immune responses known as initiation of systemic resistance (ISR) which is mediated by triggering plant immune responses elicited through pathogen-associated molecular patterns (PAMPs). The ISR triggered in the infected region of the plant leads to the development of systemic acquired resistance (SAR) throughout the plant making the plant resistant to a wide range of pathogens. A number of PGPRs including Pseudomonas and Bacillus genera have proven their ability to stimulate ISR. However, there are still some challenges in the large-scale application and acceptance of PGPR for pest and disease management. Further, we discuss the newly formulated PGPR inoculants possessing both plant growth-promoting activities and plant disease suppression ability for a holistic approach to sustaining plant health and enhancing crop productivity.
RESUMEN
The current review aims to gain knowledge on the biosynthesis and characterization of nanoparticles (NPs), their multifactorial role, and emerging trends of NPs utilization in modern science, particularly in sustainable agriculture, for increased yield to solve the food problem in the coming era. However, it is well known that an environment-friendly resource is in excessive demand, and green chemistry is an advanced and rising resource in exploring eco-friendly processes. Plant extracts or other resources can be utilized to synthesize different types of NPS. Hence NPs can be synthesized by organic or inorganic molecules. Inorganic molecules are hydrophilic, biocompatible, and highly steady compared to organic types. NPs occur in numerous chemical conformations ranging from amphiphilic molecules to metal oxides, from artificial polymers to bulky biomolecules. NPs structures can be examined by different approaches, i.e., Raman spectroscopy, optical spectroscopy, X-ray fluorescence, and solid-state NMR. Nano-agrochemical is a unification of nanotechnology and agro-chemicals, which has brought about the manufacture of nano-fertilizers, nano-pesticides, nano-herbicides, nano-insecticides, and nano-fungicides. NPs can also be utilized as an antimicrobial solution, but the mode of action for antibacterial NPs is poorly understood. Presently known mechanisms comprise the induction of oxidative stress, the release of metal ions, and non-oxidative stress. Multiple modes of action towards microbes would be needed in a similar bacterial cell for antibacterial resistance to develop. Finally, we visualize multidisciplinary cooperative methods will be essential to fill the information gap in nano-agrochemicals and drive toward the usage of green NPs in agriculture and plant science study.
Asunto(s)
Nanopartículas del Metal , Nanopartículas , Nanopartículas/química , Agricultura/métodos , Nanotecnología , Antibacterianos , Medición de Riesgo , Nanopartículas del Metal/químicaRESUMEN
In this communication we report a p-sulfonatocalix[4]arene coated ZnS quantum dots "cup type" highly stable optical probe for the detection and determination of menadione (VK(3)) with high sensitivity and selectivity. The detection of VK(3) depends on supramolecular host-guest chemistry.
Asunto(s)
Calixarenos/química , Fenoles/química , Puntos Cuánticos , Espectrometría de Fluorescencia , Sulfuros/química , Vitamina K 3/análisis , Compuestos de Zinc/química , Concentración OsmolarRESUMEN
A novel structurally simple calix[4]arene appended 8-amidoquinoline linked conjugate was synthesized and has been used as a turn-on fluorescence probe for Zn(2+) and turn off fluorescence probe for F(-). Moreover, this probe has been applied for Zn(2+) detection in blood serum upto 8.7 µM and fluoride detection upto 22 nM in waste water samples, using emission spectra.
Asunto(s)
Calixarenos , Cationes Bivalentes/sangre , Fluoruros/análisis , Aguas Residuales/química , Zinc/sangre , Colorantes Fluorescentes/química , Quinolinas/química , Espectrometría de Fluorescencia , Contaminantes Químicos del Agua/análisisRESUMEN
BACKGROUND: Mucoid degeneration of the anterior cruciate ligament is a rare pathological condition. This is a condition where the anterior cruciate ligament becomes bulky and seems like a celery stalk appearance. The most common symptom is a pain in the posterior aspect of the knee and consistent clinical finding is a mechanical block to an extension of the knee. This study aims to find out the functional outcome of arthroscopic debridement of this entity. METHODS: This is a prospective observational study from Jan 1st 2021 to Dec 31st 2021 done in 17 patients. The outcome was measured in terms of preoperative and postoperative visual analogue scale, Tegner Lysholm score and International Knee Documentation Committee score. RESULTS: Eleven females and six males were included in this study. The mean age of the patients was 51.41 years. Arthroscopic debridement of mucoid degeneration of the anterior cruciate ligament was done. Preoperative and 6 months postoperative VAS score was 8.12 and 2.00 respectively (p<0.05), preoperative and 6 months postoperative Tegner Lysholm score was 44.00 and 86.29 respectively (p<0.05), and preoperative and 6 months postoperative IKDC score was 29.06 and 79.94 respectively (p<0.05) Conclusions: Arthroscopic debridement serves as an effective way of treatment of mucoid degeneration of the anterior cruciate ligament.
Asunto(s)
Lesiones del Ligamento Cruzado Anterior , Ligamento Cruzado Anterior , Lesiones del Ligamento Cruzado Anterior/cirugía , Artroscopía/métodos , Desbridamiento/métodos , Femenino , Humanos , Articulación de la Rodilla/cirugía , Masculino , Persona de Mediana Edad , Nepal , Resultado del TratamientoRESUMEN
In the present work, we have reported a simple and cost effective colorimetric method for the detection of Fe(iii) in water. The method is based on the color change through the formation of an Fe(iii)-glycine complex at room temperature. This type of complex formation produces an intense color due to the ligand to metal charge transfer (LMCT). The rate of this type of complex formation depends appreciably on the Fe(iii) concentrations. An important aspect of the present work is that here the image analysis technique has been used successfully for the discrimination of the color obtained by the variation of the Fe(iii) concentration. The fundamental spectro-photochemical studies on the colorimetric detection of Fe(iii) by forming a metal ligand complex and thereafter the discrimination of the complex through image analysis can provide effective insight into the development of cost effective devices for the detection of liquid phase analytes.
RESUMEN
A simple, rapid, cost effective and extractive UV spectrophotometric method was developed for the determination of Gemcitabine HCl (GMCT) in bulk drug and pharmaceutical formulation. It was based on UV spectrophotometric measurements in which the drug reacts with gold nanoparticles (AuNP) and changes the original colour of AuNP and forms a dark blue coloured solution which exhibits absorption maximum at 688nm. The apparent molar absorptivity and Sandell's sensitivity coefficient were found to be 3.95×10(-5)lmol(-1)cm(-1) and 0.060µgcm(-2) respectively. Beer's law was obeyed in the concentration range of 2.0-40µgml(-1). This method was tested and validated for various parameters according to ICH guidelines. The proposed method was successfully applied for the determination of GMCT in pharmaceutical formulation (parental formulation). The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation <2%). As it is simple, cheap and less time consuming, it can be suitably applied for the estimation of GMCT in dosage forms.