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1.
PLoS Pathog ; 11(5): e1004864, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-26020637

RESUMEN

Quiescent CD4+ T cells restrict human immunodeficiency virus type 1 (HIV-1) infection at early steps of virus replication. Low levels of both deoxyribonucleotide triphosphates (dNTPs) and the biosynthetic enzymes required for their de novo synthesis provide one barrier to infection. CD4+ T cell activation induces metabolic reprogramming that reverses this block and facilitates HIV-1 replication. Here, we show that phospholipase D1 (PLD1) links T cell activation signals to increased HIV-1 permissivity by triggering a c-Myc-dependent transcriptional program that coordinates glucose uptake and nucleotide biosynthesis. Decreasing PLD1 activity pharmacologically or by RNA interference diminished c-Myc-dependent expression during T cell activation at the RNA and protein levels. PLD1 inhibition of HIV-1 infection was partially rescued by adding exogenous deoxyribonucleosides that bypass the need for de novo dNTP synthesis. Moreover, the data indicate that low dNTP levels that impact HIV-1 restriction involve decreased synthesis, and not only increased catabolism of these nucleotides. These findings uncover a unique mechanism of action for PLD1 inhibitors and support their further development as part of a therapeutic combination for HIV-1 and other viral infections dependent on host nucleotide biosynthesis.


Asunto(s)
Linfocitos T CD4-Positivos/virología , Desoxirribonucleótidos/metabolismo , Infecciones por VIH/virología , VIH-1/fisiología , Fosfolipasa D/metabolismo , Proteínas Proto-Oncogénicas c-myc/metabolismo , Replicación Viral , Apoptosis , Western Blotting , Linfocitos T CD4-Positivos/metabolismo , Proliferación Celular , Células Cultivadas , Replicación del ADN , Infecciones por VIH/inmunología , Infecciones por VIH/metabolismo , Humanos , Activación de Linfocitos , ARN Mensajero/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
2.
Am J Physiol Cell Physiol ; 309(1): C22-37, 2015 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-25924622

RESUMEN

The apolipoprotein L1 (APOL1) gene (APOL1) product is toxic to kidney cells, and its G1 and G2 alleles are strongly associated with increased risk for kidney disease progression in African Americans. Variable penetrance of the G1 and G2 risk alleles highlights the significance of additional factors that trigger or modify the progression of disease. In this regard, the effect of alternative splicing in the absence or presence of G1 or G2 alleles is unknown. In this study we investigated whether alternative splicing of non-G1, non-G2 APOL1 (APOL1 G0) affects its biological activity. Among seven APOL1 exons, exons 2 and 4 are differentially expressed in major transcripts. We found that, in contrast to APOL1 splice variants B3 or C, variants A and B1 demonstrate strong toxicity in human embryonic kidney (HEK293T) cells. Subsequently, we established that exon 4 is a major determinant of toxicity of variants A and B1 and that extracellular release of these variants is dispensable for their cytotoxicity. Although only variants A and B1 induced nuclear translocation of transcription factor EB (TFEB), a master regulator of lysosomal biogenesis and autophagy, exon 4-positive and -negative APOL1 variants stimulated perinuclear accumulation of unprocessed autophagosomes. Knockdown of endogenous TFEB did not attenuate APOL1 cytotoxicity, indicating that nuclear translocation of TFEB is dispensable for APOL1 toxicity. Our findings that a human podocyte cell line expresses exon 4-positive and -negative APOL1 transcripts suggest that these variants may play a differential role in podocyte pathology. In summary, we have identified exon 4 as a major determinant of APOL1 G0 cytotoxicity.


Asunto(s)
Apolipoproteínas/genética , Apolipoproteínas/metabolismo , Autofagia , Exones , Lipoproteínas HDL/genética , Lipoproteínas HDL/metabolismo , Podocitos/metabolismo , Transporte Activo de Núcleo Celular , Empalme Alternativo , Secuencia de Aminoácidos , Apolipoproteína L1 , Apolipoproteínas/química , Secuencia de Bases , Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice/genética , Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice/metabolismo , Regulación de la Expresión Génica , Células HEK293 , Humanos , Lipoproteínas HDL/química , Datos de Secuencia Molecular , Podocitos/patología , Interferencia de ARN , ARN Mensajero/metabolismo , Transducción de Señal , Relación Estructura-Actividad , Transcripción Genética , Transfección
3.
J Virol ; 88(1): 592-603, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-24173214

RESUMEN

Apolipoprotein L1 (APOL1) is a major component of the human innate immune response against African trypanosomes. Although the mechanism of the trypanolytic activity of circulating APOL1 has been recently clarified, the intracellular function(s) of APOL1 in human cells remains poorly defined. Like that of many genes linked to host immunity, APOL1 expression is induced by proinflammatory cytokines gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α). Additionally, IFN-γ-polarized macrophages that potently restrict HIV-1 replication express APOL1, which suggests that APOL1 may contribute to HIV-1 suppression. Here, we report that APOL1 inhibits HIV-1 replication by multiple mechanisms. We found that APOL1 protein targeted HIV-1 Gag for degradation by the endolysosomal pathway. Interestingly, we found that APOL1 stimulated both endocytosis and lysosomal biogenesis by promoting nuclear localization of transcription factor EB (TFEB) and expression of TFEB target genes. Moreover, we demonstrated that APOL1 depletes cellular viral accessory protein Vif, which counteracts the host restriction factor APOBEC3G, via a pathway involving degradation of Vif in lysosomes and by secretion of Vif in microvesicles. As a result of Vif depletion by APOL1, APOBEC3G was not degraded and reduced infectivity of progeny virions. In support of this model, we also showed that endogenous expression of APOL1 in differentiated U937 monocytic cells stimulated with IFN-γ resulted in a reduced production of virus particles. This finding supports the hypothesis that induction of APOL1 contributes to HIV-1 suppression in differentiated monocytes. Deciphering the precise mechanism of APOL1-mediated HIV-1 restriction may facilitate the design of unique therapeutics to target HIV-1 replication.


Asunto(s)
Apolipoproteínas/fisiología , Infecciones por VIH/inmunología , VIH-1/aislamiento & purificación , Inmunidad Innata , Lipoproteínas HDL/fisiología , Apolipoproteína L1 , Diferenciación Celular , Línea Celular , Endocitosis , Citometría de Flujo , Productos del Gen gag/inmunología , VIH-1/fisiología , Humanos , Interferones/inmunología , Macrófagos/inmunología , Replicación Viral
4.
J Virol ; 85(15): 7699-709, 2011 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-21613400

RESUMEN

Cholesterol plays an essential role in the life cycle of several enveloped viruses. Many of these viruses manipulate host cholesterol metabolism to facilitate their replication. HIV-1 infection of CD4(+) T cells activates the sterol regulatory element-binding protein 2 (SREBP2) transcriptional program, which includes genes involved in cholesterol homeostasis. However, the role of SREBP2-dependent transcription in HIV-1 biology has not been fully examined. Here, we identify TFII-I, a gene critical for HIV-1 transcription in activated T cells, as a novel SREBP2 target gene. We found TFII-I expression increased after HIV-1 infection or activation of human primary CD4(+) T cells. We show that inhibition of SREBP2 activity reduced TFII-I induction in response to these stimuli. More importantly, small interfering RNA (siRNA)-mediated gene silencing of either SREBP2 or TFII-I significantly reduced HIV-1 production in CD4(+) T cells. We also found that TFII-I potentiates Tat-dependent viral gene expression, consistent with a role at the level of HIV-1 transcription. Collectively, our results demonstrate for the first time that HIV-1 transcription in T cells is linked to cholesterol homeostasis through control of TFII-I expression by SREBP2.


Asunto(s)
Colesterol/metabolismo , VIH-1/genética , Homeostasis/fisiología , Activación de Linfocitos , Proteína 2 de Unión a Elementos Reguladores de Esteroles/fisiología , Linfocitos T/inmunología , Transcripción Genética/fisiología , Secuencia de Bases , Línea Celular , Cartilla de ADN , Citometría de Flujo , Humanos , Unión Proteica , Proteína 2 de Unión a Elementos Reguladores de Esteroles/metabolismo , Factores de Transcripción TFII/genética
5.
iScience ; 25(1): 103717, 2022 Jan 21.
Artículo en Inglés | MEDLINE | ID: mdl-35072009

RESUMEN

Two variants at the APOL1 gene, encoding apolipoprotein L1, account for more than 70% of the increased risk for chronic kidney disease in individuals of African ancestry. While the initiating event for APOL1 risk variant cell injury remains to be clarified, we explored the possibility of blocking APOL1 toxicity at a more upstream level. We demonstrate that deletion of the first six amino acids of exon 4 abrogates APOL1 cytotoxicity by impairing APOL1 translocation to the lumen of ER and splicing of the signal peptide. Likewise, in orthologous systems, APOL1 lethality was partially abrogated in yeast strains and flies with reduced dosage of genes encoding ER translocon proteins. An inhibitor of ER to Golgi trafficking reduced lethality as well. We suggest that targeting the MSALFL sequence or exon 4 skipping may serve as potential therapeutic approaches to mitigate the risk of CKD caused by APOL1 renal risk variants.

6.
J Virol ; 83(2): 512-21, 2009 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-18987139

RESUMEN

The human cytidine deaminase APOBEC3G (A3G) is a part of a cellular defense system against human immunodeficiency virus type 1 (HIV-1) and other retroviruses. Antiretroviral activity of A3G can be severely blunted in the presence of the HIV-1 protein Vif. However, in some cells expressing the enzymatically active low-molecular-mass form of A3G, HIV-1 replication is restricted at preintegration steps, before accumulation of Vif. Here, we show that A3G can be secreted by cells in exosomes that confer resistance to both vif-defective and wild-type HIV-1 in exosome recipient cells. Our results also suggest that A3G is the major exosomal component responsible for the anti-HIV-1 activity of exosomes. However, enzymatic activity of encapsidated A3G does not correlate with the observed limited cytidine deamination in HIV-1 DNA, suggesting that A3G-laden exosomes restrict HIV-1 through a nonenzymatic mechanism. Real-time PCR quantitation demonstrated that A3G exosomes reduce accumulation of HIV-1 reverse transcription products and steady-state levels of HIV-1 Gag and Vif proteins. Our findings suggest that A3G exosomes could be developed into a novel class of anti-HIV-1 therapeutics.


Asunto(s)
Citidina Desaminasa/inmunología , Exosomas/inmunología , VIH-1/inmunología , Desaminasa APOBEC-3G , Línea Celular , ADN Viral/genética , ADN Viral/metabolismo , Humanos , Reacción en Cadena de la Polimerasa , Proteínas Virales/biosíntesis , Replicación Viral/inmunología
7.
Exp Cell Res ; 315(11): 1875-88, 2009 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-19327352

RESUMEN

We hereby report studies that suggest a role for serum exosomes in the anchorage-independent growth (AIG) of tumor cells. In AIG assays, fetal bovine serum is one of the critical ingredients. We therefore purified exosomes from fetal bovine serum and examined their potential to promote growth of breast carcinoma cells in soft agar and Matrigel after reconstituting them into growth medium (EEM). In all the assays, viable colonies were formed only in the presence of exosomes. Some of the exosomal proteins we identified, have been documented by others and could be considered exosomal markers. Labeled purified exosomes were up-taken by the tumor cells, a process that could be competed out with excess unlabeled vesicles. Our data also suggested that once endocytosed by a cell, the exosomes could be recycled back to the conditioned medium from where they can be up-taken by other cells. We also demonstrated that low concentrations of exosomes activate MAP kinases, suggesting a mechanism by which they maintain the growth of the tumor cells in soft agar. Taken together, our data demonstrate that serum exosomes form a growth promoting platform for AIG of tumor cells and may open a new vista into cancer cell growth in vivo.


Asunto(s)
Neoplasias de la Mama/sangre , Neoplasias de la Mama/patología , Adhesión Celular/fisiología , Exosomas/fisiología , Proteínas Sanguíneas/fisiología , Neoplasias de la Mama/fisiopatología , Línea Celular Tumoral , Exosomas/ultraestructura , Femenino , Humanos , Sistema de Señalización de MAP Quinasas , Microscopía Electrónica de Transmisión , Proteómica , Espectrometría de Masas en Tándem , alfa-2-Glicoproteína-HS
8.
Sci Rep ; 9(1): 15485, 2019 10 29.
Artículo en Inglés | MEDLINE | ID: mdl-31664093

RESUMEN

APOL1 alleles G1 and G2 are associated with faster progression to lupus nephritis (LN)-associated end-stage renal disease (LN-ESRD) in African Americans. Increased levels of type I interferons (IFNs) and nucleosome-associated double-stranded DNA (dsDNA) fragments (nsDNA) are the hallmark of this disease. Here, we identify cyclic GMP-AMP synthase (cGAS) and interferon-inducible protein 16 (IFI16) as the major DNA sensors in human immortalized podocytes. We also show that nsDNA triggers the expression of APOL1 and IFNß via IRF3 activation through the cGAS/IFI16-STING pathway. We demonstrate that maximal APOL1 expression also requires the activation of type I IFN receptor (IFNAR) and STAT1 signaling triggered by IFNß produced in response to nsDNA, or by exogenous IFNß. Finally, we show that STAT1 activation is sufficient to upregulate IFI16, subsequently boosting APOL1 expression through a positive feedback mechanism. Collectively, we find that nsDNA-induced APOL1 expression is mediated by both IFNß-independent and dependent signaling pathways triggered by activation of the cGAS/IFI16-STING pathway. We propose that simultaneous inhibition of STING and the IFNAR-STAT1 pathway may attenuate IFI16 expression, reduce IFI16-cGAS cross-talk, and prevent excessive APOL1 expression in human podocytes in response to nsDNA.


Asunto(s)
Apolipoproteína L1/metabolismo , ADN/metabolismo , Proteínas de la Membrana/metabolismo , Proteínas Nucleares/metabolismo , Nucleosomas/metabolismo , Nucleotidiltransferasas/metabolismo , Fosfoproteínas/metabolismo , Podocitos/metabolismo , Transducción de Señal , Apolipoproteína L1/genética , Línea Celular Transformada , Humanos
9.
Viruses ; 11(7)2019 06 27.
Artículo en Inglés | MEDLINE | ID: mdl-31252545

RESUMEN

BACKGROUND: BK polyomavirus (BKV) reactivates from latency after immunosuppression in renal transplant patients, resulting in BKV-associated nephropathy (BKVAN). BKVAN has emerged as an important cause of graft dysfunction and graft loss among transplant patients. BKV infection in kidney transplant patients has increased over recent decades which correlates with the use of more potent immunosuppressive therapies. BKV infection of the Glomerular Vascular Unit (GVU) consisting of podocytes, mesangial cells, and glomerular endothelial cells could lead to glomerular inflammation and contribute to renal fibrosis. The effects of BKV on GVU infectivity have not been reported. METHODS: We infected GVU cells with the Dunlop strain of BKV. Viral infectivity was analyzed by microscopy, immunofluorescence, Western blot analysis, and quantitative RT-PCR (qRT-PCR). The expression of specific proinflammatory cytokines induced by BKV was analyzed by qRT-PCR. RESULTS: BKV infection of podocytes, mesangial cells, and glomerular endothelial cells was confirmed by qRT-PCR and positive staining with antibodies to the BKV VP1 major capsid protein, or the SV40 Large T-Antigen. The increased transcriptional expression of interferon gamma-induced protein 10 (CXCL10/IP-10) and interferon beta (IFNß) was detected in podocytes and mesangial cells at 96 h post-infection. CONCLUSIONS: All cellular components of the GVU are permissive for BKV replication. Cytopathic effects induced by BKV in podocytes and glomerular endothelial cells and the expression of CXCL10 and IFNß genes by podocytes and mesangial cells may together contribute to glomerular inflammation and cytopathology in BKVAN.


Asunto(s)
Virus BK/fisiología , Enfermedades Renales/virología , Glomérulos Renales/virología , Infecciones por Polyomavirus/virología , Complicaciones Posoperatorias/virología , Virus BK/genética , Citocinas/genética , Citocinas/inmunología , Humanos , Enfermedades Renales/genética , Enfermedades Renales/inmunología , Glomérulos Renales/inmunología , Trasplante de Riñón/efectos adversos , Infecciones por Polyomavirus/genética , Infecciones por Polyomavirus/inmunología , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/genética , Complicaciones Posoperatorias/inmunología , Activación Viral , Replicación Viral
10.
Sci Rep ; 8(1): 8739, 2018 06 07.
Artículo en Inglés | MEDLINE | ID: mdl-29880816

RESUMEN

APOL1 risk alleles G1 or G2 are associated with a kidney disease phenotype exclusively in people of recent African ancestry. Here we show that exon 4 encoding a part of the APOL1 signal peptide is constitutively spliced in major APOL1 transcripts expressed in kidney glomerular and tubular cells. We demonstrate that constitutive splicing of exon 4 results from a suboptimal hnRNP A1 binding motif found in exon 4. Accordingly, a robust binding of hnRNP A1 protein to a consensus hnRNP A1 cis-acting element in exon 4 results in almost complete exclusion of exon 4 from the APOL1 minigene transcripts. Blocking the 5' splice site at the exon 4/intron boundary with a specific antisense morpholino oligonucleotide excludes exon 4 from the splicing pattern of endogenous APOL1 transcripts. These transcripts are fully functional and produce APOL1 protein isoform that is not normally detectable in podocytes. Together with our previous data showing no cytotoxicity of overexpressed APOL1 isoform lacking exon 4, we propose that morpholino-induced APOL1 isoform switch may provide a new tool to identify in vivo molecular mechanism(s) by which risk alleles promote or mediate the kidney disease phenotype.


Asunto(s)
Empalme Alternativo , Apolipoproteína L1/biosíntesis , Exones , Morfolinos/farmacología , Podocitos/metabolismo , Sitios de Empalme de ARN , Apolipoproteína L1/genética , Células HEK293 , Ribonucleoproteína Nuclear Heterogénea A1/genética , Ribonucleoproteína Nuclear Heterogénea A1/metabolismo , Humanos , Enfermedades Renales/genética , Enfermedades Renales/metabolismo , Enfermedades Renales/patología , Podocitos/patología , Isoformas de Proteínas/biosíntesis , Isoformas de Proteínas/genética
11.
Virology ; 408(1): 119-27, 2010 Dec 05.
Artículo en Inglés | MEDLINE | ID: mdl-20937511

RESUMEN

Podocyte damage induced by HIV-1 is critical to the pathogenesis of HIV-1 associated nephropathy (HIVAN) and is believed to result from productive replication of the virus. Here we demonstrate that HIV-1 readily enters human podocytes by a dynamin-mediated endocytosis but does not establish productive infection. We provide evidence suggesting that viral nucleic acids and proteins detected in podocytes are delivered by viral particles internalized by the cells. Endocytosed HIV-1 is only transiently harbored by podocytes and is subsequently released to the extracellular milieu as fully infectious virus. Similarly, primary podocytes established from normal human urine do not support productive infection by HIV-1 but sustain replication of VSV-G pseudotyped virus that bypasses HIV-1 entry receptors. Moreover, transfected podocytes expressing CD4 and CXCR4 receptors support productive replication of HIV-1. This further confirms that lack of HIV-1 entry receptors is the major barrier preventing productive infection of podocytes in vitro.


Asunto(s)
VIH-1/fisiología , Podocitos/virología , Receptores del VIH/biosíntesis , Internalización del Virus , Replicación Viral , Línea Celular , Endocitosis , VIH-1/crecimiento & desarrollo , VIH-1/patogenicidad , Humanos , Receptores del VIH/genética , Liberación del Virus
12.
Virology ; 400(1): 68-75, 2010 Apr 25.
Artículo en Inglés | MEDLINE | ID: mdl-20153011

RESUMEN

Human cytidine deaminases, including APOBEC3G (A3G) and A3F, are part of a cellular defense system against retroviruses and retroelements including non-LTR retrotransposons LINE-1 (L1) and Alu. Expression of cellular A3 proteins is sufficient for inhibition of L1 and Alu retrotransposition, but the effect of A3 proteins transferred in exosomes on retroelement mobilization is unknown. Here, we demonstrate for the first time that exosomes secreted by CD4(+)H9 T cells and mature monocyte-derived dendritic cells encapsidate A3G and A3F and inhibit L1 and Alu retrotransposition. A3G is the major contributor to the inhibitory activity of exosomes, however, the contribution of A3F in H9 exosomes cannot be excluded. Additionally, we show that exosomes encapsidate mRNAs coding for A3 proteins. A3G mRNA, and less so A3F, was enriched in exosomes secreted by H9 cells. Exosomal A3G mRNA was functional in vitro. Whether exosomes inhibit retrotransposons in vivo requires further investigation.


Asunto(s)
Elementos Alu , Citidina Desaminasa/metabolismo , Citosina Desaminasa/metabolismo , Exosomas/enzimología , Exosomas/genética , Elementos de Nucleótido Esparcido Largo , Desaminasa APOBEC-3G , Linfocitos T CD4-Positivos/metabolismo , Línea Celular , Células Dendríticas/metabolismo , Humanos , Plásmidos/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Retroviridae/genética
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