RESUMEN
Natural products are important sources of chemical diversity leading to unique scaffolds that can be exploited in the discovery of new drug candidates or chemical probes. In this context, chemical and biological investigation of ferns and lycophytes occurring in Brazil is an approach adopted by our research group aiming at discovering bioactive molecules acting on neurodegeneration targets. In the present study, rosmarinic acid (RA) isolated from Blechnum brasiliense showed an in vitro multifunctional profile characterized by antioxidant effects, and monoamine oxidases (MAO-A and MAO-B) and catechol-O-methyl transferase (COMT) inhibition. RA showed antioxidant effects against hydroxyl (HO(â¢)) and nitric oxide (NO) radicals (IC50 of 29.4 and 140 µM, respectively), and inhibition of lipid peroxidation (IC50 of 19.6 µM). In addition, RA inhibited MAO-A, MAO-B and COMT enzymes with IC50 values of 50.1, 184.6 and 26.7 µM, respectively. The MAO-A modulation showed a non-time-dependent profile, suggesting a reversible mechanism of inhibition. Structural insights on RA interactions with MAO-A and COMT were investigated by molecular docking. Finally, RA (up to 5 mM) demonstrated no cytotoxicity on polymorphonuclear rat cells. Taken together, our results suggest that RA may be exploited as a template for the development of new antioxidant molecules possessing additional MAO and COMT inhibition effects to be further investigated on in vitro and in vivo models of neurodegenerative diseases.
Asunto(s)
Antioxidantes/farmacología , Apoptosis/efectos de los fármacos , Cinamatos/farmacología , Depsidos/farmacología , Helechos/química , Animales , Antioxidantes/metabolismo , Antioxidantes/uso terapéutico , Sitios de Unión , Catecol O-Metiltransferasa/química , Catecol O-Metiltransferasa/metabolismo , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Cinamatos/metabolismo , Cinamatos/uso terapéutico , Depsidos/metabolismo , Depsidos/uso terapéutico , Helechos/metabolismo , Humanos , Peróxido de Hidrógeno/toxicidad , Radical Hidroxilo/química , Radical Hidroxilo/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Simulación del Acoplamiento Molecular , Monoaminooxidasa/química , Monoaminooxidasa/metabolismo , Enfermedades Neurodegenerativas/tratamiento farmacológico , Óxido Nítrico/química , Óxido Nítrico/metabolismo , Estructura Terciaria de Proteína , Ratas , Ácido RosmarínicoRESUMEN
BACKGROUND: Ferns are a group of plants that have been little explored from a chemical and biological perspective but that have interesting potential, occurring in various parts of the world. OBJECTIVE: This work investigates the chemical profile and the biological effects of ferns from Brazil. MATERIALS AND METHODS: Analyses were performed using rapid performance liquid chromatography (RP-LC) with a diode array detector (DAD). Extracts were tested for their in vitro antioxidant activity, by the total reactive antioxidant potential method and for their antichemotactic potential, by the Boyden chamber method. Cytotoxic effects were assessed by lactate dehydrogenase levels, while the monoamine oxidase (MAO) assay was carried out using a fluorescence-based method. RESULTS: Different chemical compositions were found for the studied ferns, such as Asplenium gastonis, in which hesperidin was identified in its extract, while A. serra showed the presence of xanthone mangiferin. The most samples with highest antioxidant activity were the Asplenium serra, Lastreopsis amplissima and Cyathea dichromatolepis extracts, at 10 µg/mL. High antichemotactic activity was found for A. serra (94.06%) and Didymochlaena truncatula (93.41%), at 10 µg/mL. The extracts showed no cytotoxicity at the highest concentration. Against MAO-A, D. truncatula (82.61%), Alsophila setosa (82.21%), Cyathea phalerata (74.07%) and C. delgadii (70.32%) were the most active extracts (100 µg/mL). CONCLUSION: The hypothesis was considered that phenolics and triterpenes are responsible for these pronounced activities.