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1.
Lipids ; 34(7): 649-59, 1999 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-10478922

RESUMEN

Two cDNA clones with homology to known desaturase genes were isolated from the fungus Mortierella alpina. The open reading frame in one clone encoded 399 amino acids and exhibited delta12-desaturase activity when expressed in Saccharomyces cerevisiae in the presence of endogenous fatty acid substrate oleic acid. The insert in another clone contained an open reading frame encoding 457 amino acids and exhibited delta6-desaturase activity in S. cerevisiae in the presence of exogenous fatty acid substrate linoleic acid. Expression of the delta12-desaturase gene under appropriate media and temperature conditions led to the production of linoleic acid at levels up to 25% of the total fatty acids in yeast. When linoleic acid was provided as an exogenous substrate to the yeast cultures expressing the delta6-desaturase activity, the level of gamma-linolenic acid reached 10% of the total yeast fatty acids. Co-expression of both the delta6- and delta12-desaturase cDNA resulted in the endogenous production of gamma-linolenic acid. The yields of gamma-linolenic acid reached as high as 8% of total fatty acids in yeast.


Asunto(s)
Ácido Graso Desaturasas/genética , Mortierella/genética , Recombinación Genética , Saccharomyces cerevisiae/metabolismo , Ácido gammalinolénico/biosíntesis , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Cartilla de ADN , ADN Complementario , Ésteres , Ácidos Grasos/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Datos de Secuencia Molecular , Saccharomyces cerevisiae/genética , Homología de Secuencia de Aminoácido
2.
Lipids ; 36(2): 139-43, 2001 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-11269694

RESUMEN

Conjugated linoleic acid (CLA; 18:2), a group of positional and geometric isomers of linoleic acid (LA; 18:2n-6), has been shown to modulate immune function through its effect on eicosanoid synthesis. This effect has been attributed to a reduced production of n-6 polyunsaturated fatty acid (PUFA), the precursor of eicosanoids. Since delta6-desaturase is the rate-limiting enzyme of the n-6 PUFA production, it is our hypothesis that CLA, which has similar chemical structure to LA, interacts directly with delta6-desaturase. A unique and simple model, i.e., baker's yeast (Saccharomyces cerevisiae) transformed with fungal delta6-desaturase gene, previously established, was used to investigate the direct effect of CLA on delta6-desaturase. This model allows LA to be converted to y-linolenic acid (GLA; 18:3n-6) but not GLA to its metabolite(s). No metabolites of CLA were found in the lipids of the yeast transformed with delta6-desaturase. The inability to convert CLA to conjugated GLA was not due to the failure of yeast cells to take up the CLA isomers. CLA mixture and individual isomers significantly inhibited the activity of delta6-desaturase of the transformed yeast in vivo. Even though its uptake by the yeast was low, CLA c9,t11 isomer was found to be the most potent inhibitor of the four isomers tested, owing to its high inhibitory effect on delta6-desaturase. Since CLA did not cause significant changes in the level of delta6-desaturase mRNA, the inhibition of GLA production could not be attributed to suppression of delta6-desaturase gene expression at the transcriptional level.


Asunto(s)
Ácido Graso Desaturasas/metabolismo , Ácido Linoleico/metabolismo , Levaduras/metabolismo , Ácido Graso Desaturasas/efectos de los fármacos , Ácido Graso Desaturasas/genética , Regulación Enzimológica de la Expresión Génica , Ácido Linoleico/química , Ácido Linoleico/farmacología , Linoleoil-CoA Desaturasa , Transformación Genética , Levaduras/genética , Ácido gammalinolénico/metabolismo
3.
J Biol Chem ; 273(45): 29360-6, 1998 Nov 06.
Artículo en Inglés | MEDLINE | ID: mdl-9792636

RESUMEN

A DNA fragment with homology to Delta6-desaturases from borage and cyanobacteria was isolated after polymerase chain reaction amplification of Mortierella alpina cDNA with oligonucleotide primers corresponding to the conserved regions of known Delta6-desaturase genes. This fragment was used as a probe to isolate a cDNA clone with an open reading frame encoding 446 amino acids from a M. alpina library. Expression of this open reading frame from an inducible promoter in Saccharomyces cerevisiae in the presence of various substrates revealed that the recombinant product had Delta5-desaturase activity. The effects of growth and induction conditions as well as host strain on activity of the recombinant Delta5-desaturase in S. cerevisiae were evaluated. Expression of the M. alpina Delta5-desaturase cDNA in transgenic canola seeds resulted in the production of taxoleic acid (Delta5,9-18:2) and pinolenic acid (Delta5,9,12-18:3), which are the Delta5-desaturation products of oleic and linoleic acids, respectively.


Asunto(s)
Ácido Graso Desaturasas/metabolismo , Mortierella/enzimología , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Cartilla de ADN , ADN Complementario , Ácido Graso Desaturasas/genética , Cromatografía de Gases y Espectrometría de Masas , Datos de Secuencia Molecular , Plantas Modificadas Genéticamente , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/genética , Semillas/genética , Homología de Secuencia de Aminoácido
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