RESUMEN
Melissococcus plutonius is a fastidious honeybee pathogen, and the addition of KH(2)PO(4) to culture medium is required for its growth. Using genome sequences and a newly developed vector, we showed that mutations in genes encoding Na(+)/H(+) antiporter and cation-transporting ATPase are involved in the potassium requirement for growth.
Asunto(s)
Abejas/microbiología , ATPasas Transportadoras de Calcio/genética , Enterococcaceae/crecimiento & desarrollo , Enterococcaceae/genética , Genoma Bacteriano/genética , Potasio/metabolismo , Intercambiadores de Sodio-Hidrógeno/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Mapeo Cromosómico , Biología Computacional , Enterococcaceae/metabolismo , Prueba de Complementación Genética , Vectores Genéticos/genética , Datos de Secuencia Molecular , Mutación/genética , Análisis de Secuencia de ADNRESUMEN
We made a low calorie diet(LCD)menu which added two commercial supporting nutritional supplements to a meal. Because a conventional formula food is very expensive, the patient was not able to afford it at home. Those supplements are a total enteral formula with enriched nutrient(ACURE EN800)and vitamin-mineral rich drink(V CRESC). The contents of vitamin and mineral in this menu satisfied the dietary reference intakes, though protein was a little low. However, we could keep the price low compared to the formula food. The patient was able to switch over to home LCD therapy with the menu.