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1.
EMBO J ; 43(4): 595-614, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38267654

RESUMEN

Miro proteins are universally conserved mitochondrial calcium-binding GTPases that regulate a multitude of mitochondrial processes, including transport, clearance, and lipid trafficking. The exact role of Miro in these functions is unclear but involves binding to a variety of client proteins. How this binding is operated at the molecular level and whether and how it is important for mitochondrial health, however, remains unknown. Here, we show that known Miro interactors-namely, CENPF, Trak, and MYO19-all use a similar short motif to bind the same structural element: a highly conserved hydrophobic pocket in the first calcium-binding domain of Miro. Using these Miro-binding motifs, we identified direct interactors de novo, including MTFR1/2/1L, the lipid transporters Mdm34 and VPS13D, and the ubiquitin E3-ligase Parkin. Given the shared binding mechanism of these functionally diverse clients and its conservation across eukaryotes, we propose that Miro is a universal mitochondrial adaptor coordinating mitochondrial health.


Asunto(s)
Calcio , Mitocondrias , Humanos , Calcio/metabolismo , Mitocondrias/metabolismo , Ubiquitina-Proteína Ligasas/genética , Ubiquitina-Proteína Ligasas/metabolismo , Homeostasis , Lípidos , Proteínas Mitocondriales/metabolismo , Proteínas de Unión al GTP rho/metabolismo , Proteínas/metabolismo
2.
Mol Cell ; 77(5): 1066-1079.e9, 2020 03 05.
Artículo en Inglés | MEDLINE | ID: mdl-31902667

RESUMEN

Naturally occurring or drug-induced DNA-protein crosslinks (DPCs) interfere with key DNA transactions if not repaired in a timely manner. The unique family of DPC-specific proteases Wss1/SPRTN targets DPC protein moieties for degradation, including stabilized topoisomerase-1 cleavage complexes (Top1ccs). Here, we describe that the efficient DPC disassembly requires Ddi1, another conserved predicted protease in Saccharomyces cerevisiae. We found Ddi1 in a genetic screen of the tdp1 wss1 mutant defective in Top1cc processing. Ddi1 is recruited to a persistent Top1cc-like DPC lesion in an S phase-dependent manner to assist in the eviction of crosslinked protein from DNA. Loss of Ddi1 or its putative protease activity hypersensitizes cells to DPC trapping agents independently from Wss1 and 26S proteasome, implying its broader role in DPC repair. Among the potential Ddi1 targets, we found the core component of Pol II and show that its genotoxin-induced degradation is impaired in ddi1. We propose that the Ddi1 protease contributes to DPC proteolysis.


Asunto(s)
Daño del ADN , Reparación del ADN , ADN de Hongos/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/enzimología , Animales , ADN Nucleotidiltransferasas/genética , ADN Nucleotidiltransferasas/metabolismo , ADN-Topoisomerasas de Tipo I/genética , ADN-Topoisomerasas de Tipo I/metabolismo , ADN de Hongos/genética , Regulación Fúngica de la Expresión Génica , Hidrolasas Diéster Fosfóricas/genética , Hidrolasas Diéster Fosfóricas/metabolismo , Proteolisis , ARN Polimerasa II/genética , ARN Polimerasa II/metabolismo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Células Sf9 , Spodoptera , Transcripción Genética
3.
EMBO J ; 41(7): e109998, 2022 04 04.
Artículo en Inglés | MEDLINE | ID: mdl-35188676

RESUMEN

The organelles of eukaryotic cells differ in their membrane lipid composition. This heterogeneity is achieved by the localization of lipid synthesizing and modifying enzymes to specific compartments, as well as by intracellular lipid transport that utilizes vesicular and non-vesicular routes to ferry lipids from their place of synthesis to their destination. For instance, the major and essential phospholipids, phosphatidylethanolamine (PE) and phosphatidylcholine (PC), can be produced by multiple pathways and, in the case of PE, also at multiple locations. However, the molecular components that underlie lipid homeostasis as well as the routes allowing their distribution remain unclear. Here, we present an approach in which we simplify and rewire yeast phospholipid synthesis by redirecting PE and PC synthesis reactions to distinct subcellular locations using chimeric enzymes fused to specific organelle targeting motifs. In rewired conditions, viability is expected to depend on homeostatic adaptation to the ensuing lipostatic perturbations and on efficient interorganelle lipid transport. We therefore performed genetic screens to identify factors involved in both of these processes. Among the candidates identified, we find genes linked to transcriptional regulation of lipid homeostasis, lipid metabolism, and transport. In particular, we identify a requirement for Csf1-an uncharacterized protein harboring a Chorein-N lipid transport motif-for survival under certain rewired conditions as well as lipidomic adaptation to cold, implicating Csf1 in interorganelle lipid transport and homeostatic adaptation.


Asunto(s)
Lípidos de la Membrana , Orgánulos , Transporte Biológico , Homeostasis , Metabolismo de los Lípidos/genética , Lípidos de la Membrana/genética , Lípidos de la Membrana/metabolismo , Orgánulos/metabolismo , Fosfolípidos/genética , Fosfolípidos/metabolismo
4.
PLoS Biol ; 20(3): e3001576, 2022 03.
Artículo en Inglés | MEDLINE | ID: mdl-35320264

RESUMEN

Mitochondria and the complex endomembrane system are hallmarks of eukaryotic cells. To date, it has been difficult to manipulate organelle structures within single live cells. We developed a FluidFM-based approach to extract, inject, and transplant organelles from and into living cells with subcellular spatial resolution. The technology combines atomic force microscopy, optical microscopy, and nanofluidics to achieve force and volume control with real-time inspection. We developed dedicated probes that allow minimally invasive entry into cells and optimized fluid flow to extract specific organelles. When extracting single or a defined number of mitochondria, their morphology transforms into a pearls-on-a-string phenotype due to locally applied fluidic forces. We show that the induced transition is calcium independent and results in isolated, intact mitochondria. Upon cell-to-cell transplantation, the transferred mitochondria fuse to the host cells mitochondrial network. Transplantation of healthy and drug-impaired mitochondria into primary keratinocytes allowed monitoring of mitochondrial subpopulation rescue. Fusion with the mitochondrial network of recipient cells occurred 20 minutes after transplantation and continued for over 16 hours. After transfer of mitochondria and cell propagation over generations, donor mitochondrial DNA (mtDNA) was replicated in recipient cells without the need for selection pressure. The approach opens new prospects for the study of organelle physiology and homeostasis, but also for therapy, mechanobiology, and synthetic biology.


Asunto(s)
ADN Mitocondrial , Mitocondrias , Calcio , Homeostasis , Mitocondrias/fisiología , Orgánulos
5.
PLoS Genet ; 17(3): e1009414, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33690632

RESUMEN

Indole-3-acetic acid (IAA) is the most common, naturally occurring phytohormone that regulates cell division, differentiation, and senescence in plants. The capacity to synthesize IAA is also widespread among plant-associated bacterial and fungal species, which may use IAA as an effector molecule to define their relationships with plants or to coordinate their physiological behavior through cell-cell communication. Fungi, including many species that do not entertain a plant-associated life style, are also able to synthesize IAA, but the physiological role of IAA in these fungi has largely remained enigmatic. Interestingly, in this context, growth of the budding yeast Saccharomyces cerevisiae is sensitive to extracellular IAA. Here, we use a combination of various genetic approaches including chemical-genetic profiling, SAturated Transposon Analysis in Yeast (SATAY), and genetic epistasis analyses to identify the mode-of-action by which IAA inhibits growth in yeast. Surprisingly, these analyses pinpointed the target of rapamycin complex 1 (TORC1), a central regulator of eukaryotic cell growth, as the major growth-limiting target of IAA. Our biochemical analyses further demonstrate that IAA inhibits TORC1 both in vivo and in vitro. Intriguingly, we also show that yeast cells are able to synthesize IAA and specifically accumulate IAA upon entry into stationary phase. Our data therefore suggest that IAA contributes to proper entry of yeast cells into a quiescent state by acting as a metabolic inhibitor of TORC1.


Asunto(s)
Hongos/efectos de los fármacos , Hongos/enzimología , Ácidos Indolacéticos/farmacología , Diana Mecanicista del Complejo 1 de la Rapamicina/antagonistas & inhibidores , Inhibidores de Proteínas Quinasas/farmacología , Elementos Transponibles de ADN , Relación Dosis-Respuesta a Droga , Activación Enzimática , Hongos/genética , Ácidos Indolacéticos/química , Diana Mecanicista del Complejo 1 de la Rapamicina/metabolismo , Inhibidores de Proteínas Quinasas/química , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/enzimología , Saccharomyces cerevisiae/genética , Transducción de Señal/efectos de los fármacos
6.
PLoS Genet ; 15(3): e1008050, 2019 03.
Artículo en Inglés | MEDLINE | ID: mdl-30856164

RESUMEN

CENP-F is a large, microtubule-binding protein that regulates multiple cellular processes including chromosome segregation and mitochondrial trafficking at cytokinesis. This multiplicity of functions is mediated through the binding of various partners, like Bub1 at the kinetochore and Miro at mitochondria. Due to the multifunctionality of CENP-F, the cellular phenotypes observed upon its depletion are difficult to interpret and there is a need to genetically separate its different functions by preventing binding to selected partners. Here we engineer a CENP-F point-mutant that is deficient in Miro binding and thus is unable to localize to mitochondria, but retains other localizations. We introduce this mutation in cultured human cells using CRISPR/Cas9 system and show it causes a defect in mitochondrial spreading similar to that observed upon Miro depletion. We further create a mouse model carrying this CENP-F variant, as well as truncated CENP-F mutants lacking the farnesylated C-terminus of the protein. Importantly, one of these truncations leads to ~80% downregulation of CENP-F expression. We observe that, despite the phenotypes apparent in cultured cells, mutant mice develop normally. Taken together, these mice will serve as important models to study CENP-F biology at organismal level. In addition, because truncations of CENP-F in humans cause a lethal disease termed Strømme syndrome, they might also be relevant disease models.


Asunto(s)
Proteínas Cromosómicas no Histona/metabolismo , Proteínas de Microfilamentos/metabolismo , Proteínas Mitocondriales/metabolismo , Proteínas de Unión al GTP rho/metabolismo , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Animales , Sistemas CRISPR-Cas , Línea Celular , Proteínas Cromosómicas no Histona/química , Proteínas Cromosómicas no Histona/genética , Anomalías del Ojo/genética , Humanos , Atresia Intestinal/genética , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos DBA , Ratones Transgénicos , Microcefalia/genética , Proteínas de Microfilamentos/química , Proteínas de Microfilamentos/genética , Mitocondrias/metabolismo , Proteínas Mitocondriales/química , Proteínas Mitocondriales/genética , Mutación Puntual , Prenilación , Unión Proteica , Dominios y Motivos de Interacción de Proteínas , Proteínas de Unión al GTP rho/química , Proteínas de Unión al GTP rho/genética
7.
J Cell Sci ; 131(21)2018 10 29.
Artículo en Inglés | MEDLINE | ID: mdl-30373893

RESUMEN

The intracellular environment of eukaryotic cells is highly complex and compact. The limited volume of the cell, usually a few hundred femtoliters, is not only occupied by numerous complicated, diverse membranous and proteinaceous structures, these structures are also highly dynamic due to constant remodeling and trafficking events. Consequently, intracellular interactions are more than just opportunities to exchange molecules; they also involve components physically navigating around each other in a highly confined space. While the biochemical interactions between organelles have been intensely studied in the past decades, the mechanical properties of organelles and the physical interactions between them are only beginning to be unraveled. Indeed, recent studies show that intracellular organelles are, at times, under extreme mechanical strain both in widely used experimental systems as well as in vivo In this Hypothesis, we highlight known examples of intracellular mechanical challenges in biological systems and focus on the coping mechanisms of two important organelles, the nucleus and mitochondria, for they are the best studied in this aspect. In the case of mitochondria, we propose that ER-mitochondrial contact sites at thin cell peripheries may induce mitochondrial fission by mechanically constricting mitochondrial tubules. We also briefly discuss the mechano-responsiveness of other organelles and interesting directions for future research.


Asunto(s)
Orgánulos/metabolismo , Animales , Fenómenos Biomecánicos , Citoplasma/metabolismo , Humanos , Transporte de Proteínas
8.
Biol Chem ; 401(6-7): 811-820, 2020 05 26.
Artículo en Inglés | MEDLINE | ID: mdl-32049644

RESUMEN

Endosymbiosis, the beginning of a collaboration between an archaeon and a bacterium and a founding step in the evolution of eukaryotes, owes its success to the establishment of communication routes between the host and the symbiont to allow the exchange of metabolites. As far as lipids are concerned, it is the host that has learnt the symbiont's language, as eukaryote lipids appear to have been borrowed from the bacterial symbiont. Mitochondria exchange lipids with the rest of the cell at membrane contact sites. In fungi, the endoplasmic reticulum-mitochondria encounter structure (ERMES) is one of the best understood membrane tethering complexes. Its discovery has yielded crucial insight into the mechanisms of intracellular lipid trafficking. Despite a wealth of data, our understanding of ERMES formation and its exact role(s) remains incomplete. Here, I endeavour to summarise our knowledge on the ERMES complex and to identify lingering gaps.


Asunto(s)
Membrana Celular/metabolismo , Retículo Endoplásmico/metabolismo , Lípidos/química , Mitocondrias/metabolismo , Membrana Celular/química , Metabolismo de los Lípidos , Mitocondrias/química
9.
Mol Microbiol ; 102(5): 775-791, 2016 12.
Artículo en Inglés | MEDLINE | ID: mdl-27566438

RESUMEN

To fuel the tremendously fast replication of Plasmodium liver stage parasites, the endoplasmic reticulum (ER) must play a critical role as a major site of protein and lipid biosynthesis. In this study, we analysed the parasite's ER morphology and function. Previous studies exploring the parasite ER have mainly focused on the blood stage. Visualizing the Plasmodium berghei ER during liver stage development, we found that the ER forms an interconnected network throughout the parasite with perinuclear and peripheral localizations. Surprisingly, we observed that the ER additionally generates huge accumulations. Using stimulated emission depletion microscopy and serial block-face scanning electron microscopy, we defined ER accumulations as intricate dense networks of ER tubules. We provide evidence that these accumulations are functional subdivisions of the parasite ER, presumably generated in response to elevated demands of the parasite, potentially consistent with ER stress. Compared to higher eukaryotes, Plasmodium parasites have a fundamentally reduced unfolded protein response machinery for reacting to ER stress. Accordingly, parasite development is greatly impaired when ER stress is applied. As parasites appear to be more sensitive to ER stress than are host cells, induction of ER stress could potentially be used for interference with parasite development.


Asunto(s)
Retículo Endoplásmico/ultraestructura , Plasmodium berghei/ultraestructura , Animales , Retículo Endoplásmico/metabolismo , Estrés del Retículo Endoplásmico , Hígado/parasitología , Malaria/parasitología , Microscopía/métodos , Microscopía Electrónica de Rastreo , Plasmodium berghei/metabolismo , Proteínas Protozoarias/metabolismo , Respuesta de Proteína Desplegada
10.
Biochem Soc Trans ; 44(2): 510-6, 2016 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-27068963

RESUMEN

During mitosis, cells undergo massive deformation and reorganization, impacting on all cellular structures. Mitochondria, in particular, are highly dynamic organelles, which constantly undergo events of fission, fusion and cytoskeleton-based transport. This plasticity ensures the proper distribution of the metabolism, and the proper inheritance of functional organelles. During cell cycle, mitochondria undergo dramatic changes in distribution. In this review, we focus on the dynamic events that target mitochondria during mitosis. We describe how the cell-cycle-dependent microtubule-associated protein centromeric protein F (Cenp-F) is recruited to mitochondria by the mitochondrial Rho GTPase (Miro) to promote mitochondrial transport and re-distribution following cell division.


Asunto(s)
Mitocondrias/fisiología , Mitosis , Secuencia de Aminoácidos , Animales , Ciclo Celular , GTP Fosfohidrolasas/metabolismo , Humanos , Homología de Secuencia de Aminoácido
11.
Biochim Biophys Acta ; 1833(11): 2526-41, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-23380708

RESUMEN

Membrane-bound organelles are a wonderful evolutionary acquisition of the eukaryotic cell, allowing the segregation of sometimes incompatible biochemical reactions into specific compartments with tailored microenvironments. On the flip side, these isolating membranes that crowd the interior of the cell, constitute a hindrance to the diffusion of metabolites and information to all corners of the cell. To ensure coordination of cellular activities, cells use a network of contact sites between the membranes of different organelles. These membrane contact sites (MCSs) are domains where two membranes come to close proximity, typically less than 30nm. Such contacts create microdomains that favor exchange between two organelles. MCSs are established and maintained in durable or transient states by tethering structures, which keep the two membranes in proximity, but fusion between the membranes does not take place. Since the endoplasmic reticulum (ER) is the most extensive cellular membrane network, it is thus not surprising to find the ER involved in most MCSs within the cell. The ER contacts diverse compartments such as mitochondria, lysosomes, lipid droplets, the Golgi apparatus, endosomes and the plasma membrane. In this review, we will focus on the common organizing principles underlying the many MCSs found between the ER and virtually all compartments of the cell, and on how the ER establishes a network of MCSs for the trafficking of vital metabolites and information. This article is part of a Special Issue entitled: Functional and structural diversity of endoplasmic reticulum.


Asunto(s)
Membrana Celular/metabolismo , Retículo Endoplásmico/metabolismo , Aparato de Golgi/metabolismo , Membranas Intracelulares/metabolismo , Orgánulos/metabolismo , Animales , Humanos , Transporte de Proteínas
12.
EMBO Rep ; 13(8): 667-9, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22791025

RESUMEN

The 105th Boehringer Ingelheim Fonds International Titisee Conference 'Lipids as Organizers of Cell Membranes' took place in March 2012, in Germany. Kai Simons and Gisou Van der Goot gathered cell biologists and biophysicists to discuss the interplay between lipids and proteins in biological membranes, with an emphasis on how technological advances could help fill the gap in our understanding of the lipid part of the membrane.


Asunto(s)
Membrana Celular/metabolismo , Lípidos de la Membrana/metabolismo , Animales , Difusión , Humanos , Membrana Dobles de Lípidos/metabolismo , Proteínas de la Membrana/metabolismo , Transducción de Señal
13.
Proc Natl Acad Sci U S A ; 108(34): 14151-6, 2011 Aug 23.
Artículo en Inglés | MEDLINE | ID: mdl-21825164

RESUMEN

Mitochondria are connected to the endoplasmic reticulum (ER) through specialized protein complexes. We recently identified the ER-mitochondria encounter structure (ERMES) tethering complex, which plays a role in phospholipid exchange between the two organelles. ERMES also has been implicated in the coordination of mitochondrial protein import, mitochondrial DNA replication, and mitochondrial dynamics, suggesting that these interorganelle contact sites play central regulatory roles in coordinating various aspects of the physiology of the two organelles. Here we purified ERMES complexes and identified the Ca(2+)-binding Miro GTPase Gem1 as an integral component of ERMES. Gem1 regulates the number and size of the ERMES complexes. In vivo, association of Gem1 to ERMES required the first of Gem1's two GTPase domains and the first of its two functional Ca(2+)-binding domains. In contrast, Gem1's second GTPase domain was required for proper ERMES function in phospholipid exchange. Our results suggest that ERMES is not a passive conduit for interorganellar lipid exchange, but that it can be regulated in response to physiological needs. Furthermore, we provide evidence that the metazoan Gem1 ortholog Miro-1 localizes to sites of ER-mitochondrial contact, suggesting that some of the features ascribed to Gem1 may be evolutionarily conserved.


Asunto(s)
Secuencia Conservada , Retículo Endoplásmico/metabolismo , GTP Fosfohidrolasas/metabolismo , Mitocondrias/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/enzimología , Animales , Células COS , Cardiolipinas/biosíntesis , Chlorocebus aethiops , Saccharomyces cerevisiae/citología , Tinción con Nitrato de Plata
15.
FEBS Lett ; 598(10): 1292-1298, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38268324

RESUMEN

Lipid trafficking is critical for the biogenesis and expansion of organelle membranes. Lipid transport proteins (LTPs) have been proposed to facilitate lipid transport at contact sites between organelles. Despite the fundamental importance of LTPs in cell physiology, our knowledge on the mechanisms of interorganelle lipid distribution remains poor due to the scarcity of assays to monitor lipid flux in vivo. In this review, we highlight the recent development of a versatile method named METALIC (Mass tagging-Enabled Tracking of Lipids in Cells), which uses a combination of enzymatic mass tagging and mass spectrometry to track lipid flux between organelles inside living cells. We discuss the methodology, its distinct advantages, limitations as well as its potential to unearth the pipelines of lipid transport and LTP function in vivo.


Asunto(s)
Metabolismo de los Lípidos , Humanos , Transporte Biológico , Animales , Espectrometría de Masas/métodos , Orgánulos/metabolismo , Lípidos/química
16.
Nat Commun ; 15(1): 6601, 2024 Aug 04.
Artículo en Inglés | MEDLINE | ID: mdl-39097570

RESUMEN

Understanding protein function is pivotal in comprehending the intricate mechanisms that underlie many crucial biological activities, with far-reaching implications in the fields of medicine, biotechnology, and drug development. However, more than 200 million proteins remain uncharacterized, and computational efforts heavily rely on protein structural information to predict annotations of varying quality. Here, we present a method that utilizes statistics-informed graph networks to predict protein functions solely from its sequence. Our method inherently characterizes evolutionary signatures, allowing for a quantitative assessment of the significance of residues that carry out specific functions. PhiGnet not only demonstrates superior performance compared to alternative approaches but also narrows the sequence-function gap, even in the absence of structural information. Our findings indicate that applying deep learning to evolutionary data can highlight functional sites at the residue level, providing valuable support for interpreting both existing properties and new functionalities of proteins in research and biomedicine.


Asunto(s)
Biología Computacional , Proteínas , Proteínas/metabolismo , Proteínas/química , Biología Computacional/métodos , Aprendizaje Profundo , Bases de Datos de Proteínas , Algoritmos , Humanos
17.
J Cell Sci ; 123(Pt 9): 1389-93, 2010 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-20410371

RESUMEN

Organelles are separate yet interdependent units of eukaryotic cells. They provide an appropriate milieu for the catalysis of many biochemical reactions, and they must establish physical links to communicate and exchange metabolites throughout the cell. Interorganelle communication is an important, yet still poorly understood, aspect of cell biology. We recently identified a protein complex that we refer to as ERMES [endoplasmic reticulum (ER)-mitochondria encounter structure], the main function of which is to provide a tethering force between the ER and the mitochondria. This complex, composed of both ER and mitochondrial transmembrane proteins, is located at the interface of the two organelles and serves to zipper them together. Previous work had implicated several ERMES components in many different physiological processes. The identification of ERMES as an interorganelle tether allows us to re-examine the amply documented phenotypic consequences of the loss of this complex in the light of this new function, thus providing a unique opportunity to assess the physiological relevance of ER-mitochondria junctions. These phenotypes hint at a broad role for ER-mitochondria connections in regulating mitochondrial and cell physiology. In this Hypothesis, we speculate on the potential role of ER-mitochondria connections as hubs in regulating several aspects of mitochondrial biology, including the regulation of mitochondrial membrane biosynthesis, genome replication, Ca(2+) signaling and protein import. Finally, we discuss how cells might use ER-mitochondria communication to fine-tune these processes according to their metabolic needs.


Asunto(s)
Retículo Endoplásmico/metabolismo , Mitocondrias/metabolismo , Animales , Señalización del Calcio , Replicación del ADN/genética , Genoma Mitocondrial/genética , Humanos , Fosfolípidos/metabolismo , Transporte de Proteínas
18.
Biochem Soc Trans ; 40(2): 445-50, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22435828

RESUMEN

Cellular organelles need to communicate in order to co-ordinate homoeostasis of the compartmentalized eukaryotic cell. Such communication involves the formation of membrane contact sites between adjacent organelles, allowing privileged exchange of metabolites and information. Using a synthetic protein designed to artificially tether the ER (endoplasmic reticulum) to mitochondria, we have discovered a yeast protein complex naturally involved in establishing and maintaining contact sites between these two organelles. This protein complex is physiologically involved in a plethora of mitochondrial processes, suggesting that ER-mitochondria connections play a central co-ordinating role in the regulation of mitochondrial biology. Recent biochemical characterization of this protein complex led to the discovery that GTPases of the Miro family are part of ER-mitochondria connections. The yeast Miro GTPase Gem1 localizes to ER-mitochondria interface and influences the size and distribution of mitochondria. Thus Miro GTPases may serve as regulators of the ER-mitochondria connection.


Asunto(s)
Retículo Endoplásmico/metabolismo , Mitocondrias/metabolismo , Complejos Multiproteicos/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , GTP Fosfohidrolasas/metabolismo
19.
PLoS Biol ; 7(9): e1000181, 2009 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19721697

RESUMEN

In mammals, many aspects of behavior and physiology, and in particular cellular metabolism, are coordinated by the circadian timing system. Molecular clocks are thought to rely on negative feedback loops in clock gene expression that engender oscillations in the accumulation of transcriptional regulatory proteins, such as the orphan receptor REV-ERBalpha. Circadian transcription factors then drive daily rhythms in the expression of clock-controlled output genes, for example genes encoding enzymes and regulators of cellular metabolism. To gain insight into clock output functions of REV-ERBalpha, we carried out genome-wide transcriptome profiling experiments with liver RNA from wild-type mice, Rev-erbalpha knock-out mice, or REV-ERBalpha overexpressing mice. On the basis of these genetic loss- and gain-of-function experiments, we concluded that REV-ERBalpha participates in the circadian modulation of sterol regulatory element-binding protein (SREBP) activity, and thereby in the daily expression of SREBP target genes involved in cholesterol and lipid metabolism. This control is exerted via the cyclic transcription of Insig2, encoding a trans-membrane protein that sequesters SREBP proteins to the endoplasmic reticulum membranes and thereby interferes with the proteolytic activation of SREBPs in Golgi membranes. REV-ERBalpha also participates in the cyclic expression of cholesterol-7alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in converting cholesterol to bile acids. Our findings suggest that this control acts via the stimulation of LXR nuclear receptors by cyclically produced oxysterols. In conclusion, our study suggests that rhythmic cholesterol and bile acid metabolism is not just driven by alternating feeding-fasting cycles, but also by REV-ERBalpha, a component of the circadian clockwork circuitry.


Asunto(s)
Ácidos y Sales Biliares/metabolismo , Ritmo Circadiano/fisiología , Miembro 1 del Grupo D de la Subfamilia 1 de Receptores Nucleares/metabolismo , Transducción de Señal , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo , Animales , Relojes Biológicos/genética , Relojes Biológicos/fisiología , Western Blotting , Colesterol/metabolismo , Colesterol 7-alfa-Hidroxilasa/genética , Colesterol 7-alfa-Hidroxilasa/metabolismo , Ritmo Circadiano/genética , Análisis por Conglomerados , Retículo Endoplásmico/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Perfilación de la Expresión Génica , Aparato de Golgi/metabolismo , Homeostasis , Hígado/metabolismo , Receptores X del Hígado , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Ratones , Ratones Noqueados , Ratones Transgénicos , Miembro 1 del Grupo D de la Subfamilia 1 de Receptores Nucleares/genética , Análisis de Secuencia por Matrices de Oligonucleótidos , Receptores Nucleares Huérfanos/genética , Receptores Nucleares Huérfanos/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/genética , Triglicéridos/metabolismo
20.
Methods Mol Biol ; 2477: 349-379, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35524127

RESUMEN

Genome-wide transposon mutagenesis followed by deep sequencing allows the genome-wide mapping of growth-affecting loci in a straightforward and time-efficient way.SAturated Transposon Analysis in Yeast (SATAY) takes advantage of a modified maize transposon that is highly mobilizable in S. cerevisiae. SATAY allows not only the genome-wide mapping of genes required for growth in select conditions (such as genetic interactions or drug sensitivity/resistance), but also of protein sub-domains, as well as the creation of gain- and separation-of-function alleles. From strain preparation to the mapping of sequencing reads, we detail all the steps for the making and analysis of SATAY libraries in any S. cerevisiae lab, requiring only ordinary equipment and access to a Next-Gen sequencing platform.


Asunto(s)
Elementos Transponibles de ADN , Saccharomyces cerevisiae , Alelos , Mapeo Cromosómico , Elementos Transponibles de ADN/genética , Mutagénesis Insercional , Saccharomyces cerevisiae/genética
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