The delivery of foreign genes into fertilized fish eggs using high-velocity microprojectiles.

Fertilized eggs of loach (Misgurnus fossilis), rainbow trout (Salmo gairdneri) and zebrafish (Brachydanio rerio) were bombarded with high-velocity tungsten microprojectiles covered with plasmid DNA containing sequences of beta-galactosidase and neomycin phosphotransferase genes. About 70% of the eggs survived the bombardment. The activity of both transferred genes was revealed in the fish developed from the bombarded eggs. Neomycin phosphotransferase gene sequences were detected by means of PCR amplification and Southern hybridization in the total DNA of zebrafish that survived after G418 treatment.

[High velocity mechanical injection of foreign DNA into fish oocytes]. / Vysokoskorostnaia mekhanicheskaia in'ektsiia chuzherodnoi DNK v iaitsekletki ryby.

High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died as a result of bombardment. Experiments revealed marked activity of both enzymes in developing fishes. Neo gene DNA sequences were found in total danio DNA using PCR technique.

[The search for factors limiting growth rate]. / Poisk faktorov, ogranichivaiushchikh skorost' rosta.

Search for a factor limiting the growth rate is described the salmon fry taken as an example. A simple dependence between the specific growth rate and DNA content (of the nuclei) in muscles was found. Age changes in the growth rate are due to the fact that the appearance of new nuclei lags behind the growth of muscle cells. A few models were proposed on this basis which correspond to various growth types.