RESUMEN
Glechoma hederacea L. is a medicinal plant that is known in traditional medicine for its anti-inflammatory, antibacterial, antiviral, and anticancer properties. This study evaluated the potential for commercial production of G. hederacea and compared the chemical composition and activity of 70% ethanol extracts and steam-distilled essential oils from wild-grown and cultivated G. hederacea collected in different harvesting periods. The main compounds identified in the 70% ethanol extracts were phenolic acids (chlorogenic and rosmarinic acids) and flavonoid O-glycosides. The essential oil varied in the three accessions in the range of 0.32-2.98 mL/kg-1 of dry weight. The extracts possessed potent antioxidant and anti-inflammatory properties in LPS-treated bone-marrow-derived macrophages. The results of flow cytometry show that extracts from different vegetation periods reduced the conversion of macrophages to the proinflammatory phenotype M1. The chemical composition varied the most with the different harvesting periods, and the most suitable periods were the flowering and vegetative phases for the polyphenolic compounds and essential oils, respectively. G. hederacea can be successfully grown under organic farming conditions, and cultivation does not significantly affect the chemical composition and biological activity compared to wild-grown plants.
RESUMEN
The effect of cultivation practises on both the phytochemical profile and biological activity of aqueous ethanol extracts of Chelidonium majus L. was studied. Extracts were prepared from aerial parts of the same plant population collected in the wild and grown under organic farming conditions. Both qualitative and quantitative analyses of alkaloids and flavonoid derivatives were performed by LC/MS methods, and the cytotoxicity of lyophilised extracts was studied in B16-F10, HepG2, and CaCo-2 cells. Coptisine was the dominant alkaloid of extracts prepared from wild-grown plants, whereas after cultivation, chelidonine was the most abundant alkaloid. The total alkaloid content was significantly increased by cultivation. Ten flavonol glycoconjugates were identified in C. majus extracts, and quantitative analysis did not reveal significant differences between extracts prepared from wild-grown and cultivated specimens. Treatment with C. majus extracts resulted in a dose-dependent increase in cytotoxicity in all three cell lines. The extracts prepared from cultivated specimens showed higher cytotoxicity than the extracts prepared from wild-grown plants. The strongest cytotoxic effect of cultivated C. majus was observed in B16-F10 cells (IC50 = 174.98 ± 1.12 µg/mL). Cultivation-induced differences in the phytochemical composition of C. majus extracts resulted in significant increases in the cytotoxic activities of the preparations.
RESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Prunus padus L. has been traditionally used in European ethnomedicine as a treatment for internal and external purposes and is mainly used to reduce inflammation, pain and fever. The activities of P. padus flower extracts are not well characterized, and additional experimental studies at the molecular level are needed to confirm the ethnobotanical findings. AIM OF THE STUDY: To assess the potential of P. padus flower extract (PPFE) as a source of bioactive compounds through the characterization of its chemical composition and antioxidant, anti-collagenase, and anti-inflammatory activities. MATERIALS AND METHODS: The ethanolic extract (1:10 w/v in ethanol solution) from P. padus flowers was subjected to phytochemical analysis and evaluation of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Anti-collagenase activity was determined using a spectrophotometric method in vitro. The effect of PPFE on inflammation was evaluated by measuring specific markers using flow cytometry and assessing pro-inflammatory cytokine (IL-6) release by bone marrow-derived macrophages (BMDMs) ex vivo. RESULTS: The major components of the ethanolic extract of P. padus flowers were quercetin diglycosides, chlorogenic acid and N',Nâ³-dicaffeoyl,Nâ´-coumaroyl spermidine. The total phenolic content of PPFE was 85.19 mg GAE/g extract, and the EC50 value in the DPPH assay was 0.55 mg/ml. PPFE exhibited the ability to inhibit collagenase activity in a dose-dependent manner. Preincubation of BMDMs with PPFE reduced the population of M1 (pro-inflammatory) and increased the population of M2 (anti-inflammatory) macrophages. Furthermore, PPFE decreased pro-inflammatory cytokine IL-6 release from BMDMs. CONCLUSIONS: PPFE is a rich source of bioactive compounds and possesses considerable anti-inflammatory properties, supporting its use in ethnomedicine for the reduction of inflammatory processes.