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1.
New Phytol ; 239(5): 1903-1918, 2023 09.
Artículo en Inglés | MEDLINE | ID: mdl-37349864

RESUMEN

The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer-za.227 and cer-ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown. Cuticular waxes and permeabilities were measured in cer-za.227 and cer-ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer-za alleles were generated by genome editing. The CER-ZA protein was characterized after expression in yeast and Arabidopsis cer4-3. Cer-za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl-CoA reductase (FAR1). The cer-ye.267 mutation is located to HORVU4Hr1G063420 encoding ß-ketoacyl-CoA synthase (KAS1) and is allelic to cer-zh.54. The amounts of intracuticular waxes were strongly decreased in cer-ye.267. The cuticular water loss and permeability of cer-za.227 were similar to wild-type (WT), but were increased in cer-ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration. The differential decrease in intracuticular waxes between cer-za.227 and cer-ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.


Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Hordeum , Proteínas de Saccharomyces cerevisiae , Hordeum/genética , Hordeum/metabolismo , Hojas de la Planta/metabolismo , Agua/metabolismo , Saccharomyces cerevisiae/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Ceras/metabolismo , Mutación/genética , Epidermis de la Planta/metabolismo , Proteínas Nucleares/metabolismo , Proteínas de Arabidopsis/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Proteínas Inhibidoras de las Quinasas Dependientes de la Ciclina/genética , Proteínas Inhibidoras de las Quinasas Dependientes de la Ciclina/metabolismo
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