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Amoxicillin, a member of the penicillin family, is primarily utilized for the treatment of various bacterial infections affecting ears, nose, throat, urinary tract, and skin. Given its widespread application in medicine, agriculture, environment, and food industry, the precise and sensitive detection of amoxicillin is important. This study introduces a novel approach to developing a sensitive and selective fluorescent aptasensor relying on fluorescence resonance energy transfer (FRET) for the specific detection of amoxicillin. The carboxyfluorescein-labeled aptamer serves as a energy donor, while MXene functions as an energy acceptor, and acting as a quencher. To achieve optimal detection efficiency, a dual optimization strategy utilizing RSM-CCD and ANN-GA was used to fine-tune experimental conditions. The fluorescence measurements revealed an expansive linear range extending from 100 to 2400 ng mL-1, accompanied by an exceptionally low detection limit of 1.53 ng mL-1. Additionally, it shows an excellent selectivity towards amoxicillin over other antibiotics commonly found in water matrices. The aptasensor demonstrates good stability and reproducibility; effectiveness of the aptasensor was validated by testing in real water samples. This remarkable sensitivity and broad dynamic range affirm the efficacy aptasensor in accurately detecting varying concentrations of amoxicillin in wastewater bodies.
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Amoxicilina , Técnicas Biosensibles , Amoxicilina/análisis , Amoxicilina/química , Transferencia Resonante de Energía de Fluorescencia/métodos , Aptámeros de Nucleótidos/química , Fluorescencia , Contaminantes Químicos del Agua/análisis , Agua/químicaRESUMEN
This study explores the biosynthesis of silver nanoparticles (AgNPs) using the Streptomyces tuirus S16 strain, presenting an eco-friendly alternative to mitigate the environmental and health risks of chemical synthesis methods. It focuses on optimizing medium culture conditions, understanding their physicochemical properties, and investigating their potential photothermal-based antibacterial application. The S16 strain was selected from soils contaminated with heavy metals to exploit its ability to produce diverse bioactive compounds. By employing the combination of Response Surface Methodology (RSM) and Artificial Neural Network (ANN)-Genetic Algorithm (GA) strategies, we optimized AgNPs synthesis, achieving an improvement of nearly 2.45 times the initial yield under specific conditions (Bennet's medium supplemented with glycerol [5 g/L] and casamino-acid [3 g/L] at 30 °C for 72 h). A detailed physicochemical characterization was conducted. Notably, the AgNPs were well dispersed, and a carbonaceous coating layer on their surface was confirmed using energy-dispersive X-ray spectroscopy. Furthermore, functional groups were identified using Fourier-transform infrared spectroscopy, which helped enhance the AgNPs' stability and biocompatibility. AgNPs also demonstrated efficient photothermal conversion under light irradiation (0.2 W/cm2), with temperatures increasing to 41.7 °C, after 30 min. In addition, treatment with light irradiation of E. coli K-12 model effectively reduced the concentration of AgNPs from 105 to 52.5 µg/mL, thereby enhancing the efficacy of silver nanoparticles in contact with the E. coli K-12.
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Antibacterianos , Nanopartículas del Metal , Metales Pesados , Redes Neurales de la Computación , Plata , Microbiología del Suelo , Plata/farmacología , Plata/química , Nanopartículas del Metal/química , Antibacterianos/farmacología , Antibacterianos/química , Algoritmos , Streptomyces/metabolismo , Streptomyces/efectos de la radiación , Pruebas de Sensibilidad Microbiana , Contaminantes del Suelo , Escherichia coli/efectos de los fármacos , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Azithromycin (AZM), an antibacterial considered one of the most consumed drugs, especially during the period against the Covid 19 pandemic, and it is one of the persistent contaminants that can be released into aquatic ecosystems. The purpose of this study is to determine the efficacy of a Fenton-like process (chlorine/iron) for the degradation of AZM in an aqueous medium by determining the impact of several factors (the initial concentration of (FeSO4, NaClO, pollutant), and the initial pH) on the degradation rate. The Response Surface Methodology (RSM) based on the Box-Wilson design as well as the Artificial Neural Network (ANN) modeling combined with a genetic algorithm (GA) approaches were used to determine the optimal levels of the selected variables and the optimal rate of degradation. The quadratic model of multi-linear regression developed indicated that the optimal conditions were a concentration of chlorine of 600 µM, the concentration of AZM is 32.8 mg/L, the mass of the catalyst FeSO4 is 3.5 mg and a pH of 2.5, these optimal values gave a predicted and experimental yield of 64.05% and 70% respectively, the lack of fit test in RSM modeling (F0 = 3.31 which is inferior to Fcritic (0.05, 10.4) = 5.96) indicates that the true regression function is not linear therefore, the ANN-GA modeling as non-linear regression indicated that the optimal conditions were a concentration of chlorine of 256 µM, the concentration of AZM is 5 mg/L, the mass of the catalyst FeSO4 is 9.5 mg and a pH of 2.8, these optimal values gave a predicted and experimental yield of 79.69% and close to 80% respectively, Furthermore, biotoxicity tests were conducted to confirm the performance of our process using bio-indicators called daphnia (Daphnia magna), which demonstrated the efficacy of the like-Fenton process after 4 h of degradation.
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Tratamiento Farmacológico de COVID-19 , Daphnia , Animales , Azitromicina/toxicidad , Cloro/toxicidad , Ecosistema , Redes Neurales de la Computación , AguaRESUMEN
The present work aims the synthesis of a novel, low cost, and environmentally friendly PANI/PVA-CuNi composite by chemical oxidative polymerization of aniline monomer and polyvinyl alcohol (PVA) as film matrix; several percentages of copper (Cu) and Nickel (Ni) were used. UV-Visible spectroscopy, FTIR, SEM-EDX, and TGA were used to characterize the nanocomposites. While PANI/PVA-CuNi nanocomposites were investigated in adsorption experiments of methylene blue (MB) under different controlled conditions (time reaction, adsorbent dosage, initial dye concentration, stirring speed, temperature, and pH of the medium) also various kinetic models were employed to evaluate the efficiency of the adsorption. The results revealed that the10 mg of PANI/PVA-Cu50Ni50 and PANI/PVA-Ni composites Catalyst removed (94% and 93% of methylene blue in 180 min respectively at 10-5 M initial concentration of dye, pH of 13, stirring speed of 150 rpm, the temperature of 301 k. the kinetics data were properly fitted with the pseudo second-order model with a correlation coefficient of 0.98262 and 0.95881 using PANI/PVA-Cu50Ni50 and PANI/PVA-Ni, respectively.
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Alcohol Polivinílico , Contaminantes Químicos del Agua , Alcohol Polivinílico/química , Azul de Metileno/química , Contaminantes Químicos del Agua/química , Temperatura , Colorantes/química , Adsorción , Cinética , Concentración de Iones de HidrógenoRESUMEN
Conductive crystalline polypyrrole (Cryst-PPy), Nickel-polypyrrole (Ni-PPy), and copper- polypyrole (Cu-PPy) hybrid materials were prepared using a chemical polymerization method in an aqueous solution. Part I was focused on the Chemical synthesis of Cryst-PPy powder from an organic medium. Cryst-PPy powder was successfully synthesized by chemical route from an organic medium of acetonitrile with polyethylene oxide as a stabilizing agent and oxidizing agent like potassium peroxydisulfate. The morphological study was showed the presence of spherical nanoparticles and cubic microparticles giving rise to a denser structure of PPy. In the second part, the based electrodes composites were examined in the oxidation of phenol by an electrochemical process in an alkaline medium. To follow the yield of phenol degradation at the alkaline solution, UV-visible analysis was performed at the following operating conditions: current density of 0.58 mA cm-2, phenol initial concentration of 0.150 M and for 3 h processing; the rate of phenol elimination was 56%, 38% and 28% for Cu-PPy, Ni-PPy, and pure PPy electrodes respectively. Thus, can be found that the doped Cu-PPy electrodes electrode is a new material with high electrochemical oxidation ability for phenol degradation in aqueous solutions.
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Increasing antimicrobial resistance has led to use of novel technologies such as nanomaterials and nanocomposites that have shown effective antimicrobial and/or antifungal activities against several gram-positive and gram-negative bacteria. There have been limited studies on antimicrobial properties of the combined polymer nanocomposites with transitional bimetallic nanoparticles such as nickel (Ni) and copper (Cu). Thus, the main objective of this study was to synthesis, characterize and investigate the antibacterial and antifungal properties of NiCu-PANI/PVA nanocomposite. The nanocomposite films with different amount of Ni and Cu salts were synthesized by chemical oxidative polymerization of polyaniline using HCl as oxidant and PVA as a stabilizer. Optical, chemical composition, and morphological characteristics as well as thermal stability were evaluated using UV-Visible, FTIR, SEM-EDX, and TGA analyses. Antimicrobial properties were then determined using the disc diffusion assay against gram-negative bacteria (i.e., Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 700603, Proteus sp.,) and gram-positive bacteria (i.e., Staphylococcus aureus ATCC 2593). Fungal plant pathogens including Aspergillus niger and Fusarium oxysporum f. sp. pisi were also evaluated for determination of antifungal activity of NiCu-PANI/PVA films. Among the synthesized films, Ni65Cu35-PANI/PVA showed excellent antibacterial activity against all the bacteria strains examined in this study. The diameters of inhibition zones for Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Proteus sp., and Staphylococcus aureus ATCC 2593 were 23, 23, 17, and 18 mm, respectively indicating good antibacterial activities. Additionally, NiCu-PANI/PVA, particularly the films with higher Cu intake, showed better antifungal activity against Fusarium oxysporum f. sp. pisi. However, NiCu-PANI/PVA was ineffective against Aspergillus niger.
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Antiinfecciosos , Nanocompuestos , Compuestos de Anilina , Antibacterianos/farmacología , Antifúngicos/farmacología , Bacterias Gramnegativas , Bacterias Grampositivas , Pruebas de Sensibilidad Microbiana , Estrés Oxidativo , PolimerizacionRESUMEN
The photocatalytic degradation of pharmaceutical micropollutants of Penicillin G (PG) was investigated in a photoreactor at a laboratory scale. The impact of type of catalyst, pH, and initial concentration of PG were studied. Maximum removal efficiency was obtained at pH = 6.8, [ZnO]0 = 0.8 g L-1, and [PG]0 = 5 mg L-1 and reaction time of 150 min. The addition of persulfate sodium (PPS) enhanced the efficiency of the photocatalytic reaction. The efficiency of photolysis process in the presence of PPS was significantly improved to 72.72% compared to the classical photocatalysis system (56.71%). Optimum concentration of PPS to completely degraded PG was found to be 500 mg L-1. The QuEChERS extraction, GC-MS/MS method, and concentration technique showed favorable performance identification of the possible mechanism of PG degradation pathway. Toxicity of PG and its by-products were evaluated using microbioassays assessment based on nine selected bacterial strains. Results confirmed the effectiveness of the implemented system and its safe use via the bacteria Bacillus subtilis, which has illustrated significant activity. Due to the high efficiency, facility benefits, and low-cost of the suggested process, the process can be considered for the degradation of various pharmaceutical contaminants in pharmaceutical industry treatment under the optimal conditions.
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Espectrometría de Masas en Tándem , Contaminantes Químicos del Agua , Catálisis , Cinética , Penicilina G , FotólisisRESUMEN
This article was focused on the elaboration of NiFe-Polyaniline glucose sensors via electrochemical technique. Firstly, the PANi (polyaniline) fibers were synthesized by oxidation of the monomer aniline on FTO (fluorine tin oxide) substrate. Secondly, the Nickel-Iron nanoparticles (NiFe (NPs)) were obtained by the Chronoamperometry method on the Polyaniline surface. The NiFe-PANi hybrid electrode was characterized by scanning electron microscopy (SEM), force atomic microscopy (AFM), Fourier-transformed infrared (FTIR), and X-ray diffraction (XRD). The electrochemical glucose sensing performance of the NiFe alloy nanoparticle was studied by cyclic voltammetry and amperometry. The fabricated glucose sensor Ni-Fe hybrid material exhibited many remarkable sensing performances, such as low-response time (4 s), sensitivity (1050 µA mM-1 cm-2), broad linear range (from 10 µM -1 mM), and low limit of detection (LOD) (0.5 µM, S/N = 3). The selectivity, reliability, and stability of the NiFe hybrid material for glucose oxidation were also investigated. All the results demonstrated that the NiFe-PANi/FTO hybrid electrode is very promising for application in electrochemical glucose sensing.
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Compuestos de Anilina/química , Técnicas Biosensibles/instrumentación , Glucosa/análisis , Hierro/química , Nanopartículas del Metal/química , Níquel/química , Técnicas Electroquímicas/métodos , Límite de Detección , Microscopía de Fuerza Atómica , Microscopía Electrónica de Rastreo , Reproducibilidad de los Resultados , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos XRESUMEN
AIM: The Developmental Origin of Health and Disease refers to the concept that early exposure to toxicants or nutritional imbalances during perinatal life induces changes that enhance the risk of developing noncommunicable diseases in adulthood. Patients/materials & methods: An experimental model with an adult chronic germ cell death phenotype resulting from exposure to a xenoestrogen was used. RESULTS: A reciprocal negative feedback loop involving decreased EZH2 protein level and increased miR-101 expression was identified. In vitro and in vivo knockdown of EZH2 induced an apoptotic process in germ cells through increased levels of apoptotic factors (BIM and BAD) and DNA repair alteration via topoisomerase 2B deregulation. The increased miR-101 levels were observed in the animal blood, meaning that miR-101 may be a part of a circulating mark of germ cell death. CONCLUSION: miR-101-EZH2 pathway deregulation could represent a novel pathophysiological epigenetic basis for adult germ cell disease with environmental and developmental origins.
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Proteína Potenciadora del Homólogo Zeste 2/metabolismo , Células Germinativas/metabolismo , MicroARNs/metabolismo , Animales , Apoptosis , Proteínas Reguladoras de la Apoptosis/metabolismo , Muerte Celular , Daño del ADN , Epigénesis Genética , Estradiol/análogos & derivados , Estradiol/farmacología , Infertilidad Masculina/genética , Masculino , Ratas , Testículo/efectos de los fármacos , Testículo/patologíaRESUMEN
MiRNAs (microRNAs) are single-stranded non-coding RNAs of approximately 21-23 nucleotides in length whose main function is to inhibit gene expression by interfering with mRNA processes. MicroRNAs suppress gene expression by affecting mRNA (messenger RNAs) stability, targeting the mRNA for degradation, or both. In this review, we have examined how microRNA expression could be altered following exposure to chemicals and how they could represent appropriate tissue and more interestingly circulating biomarkers. Among the key questions before using the microRNA for evaluation of risk toxicity, it remains still to clarify how they could be causally involved in the adverse effects and how stable their changes are.
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Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos/genética , MicroARNs/genética , MicroARNs/metabolismo , ARN Mensajero/metabolismo , Xenobióticos/toxicidad , Animales , Biomarcadores , Expresión Génica , Humanos , MicroARNs/sangre , ARN Mensajero/genética , Medición de RiesgoRESUMEN
Mycotoxin zearalenone (ZEN) is a cereal contaminant produced by various species of Fusarium fungi. When interacting with estrogen receptors, ZEN leads to animal fertility disturbances and other reproductive pathologies. Few data are available on the effects of perinatal exposure to ZEN, particularly in the blood-testis barrier. The aim of this study was to assess the impact of ZEN in adult rats exposed neonatally. We focused on the expression and cellular localization of major ABC transporters expressed in adult rat testis, comparing ZEN effects with those of Estradiol Benzoate (EB) neonatal exposure. Dose-dependent and long term modulations of mRNA and protein levels of Abcb1, Abcc1, Abcg2, Abcc4 and Abcc5 were observed, along with Abcc4 protein cellular delocalization. ZEN exposure of SerW3 Sertoli cells showed modulation of Abcb1, Abcc4 and Abcc5. Comparison with EB exposure showed similar modulation profiles for Abcg2 but differential modulations for Abcb1, Abcc1, Abcc4 and Abcc5 in vivo, and a similar profile for Abcb1 modulation by ZEN and EB, but differential modulation for Abcc4 and Abcc5 in vitro. ZEN and EB effects were inhibited by in vitro addition of the pure anti-estrogen ICI 182.780, suggesting the at least partial implication of ZEN estrogenic activity in these modulations. These results suggested that ZEN neonatal exposure could affect the exposure of testis to ABC transporter substrates, and negatively influence spermatogenesis and male fertility.
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Transportadoras de Casetes de Unión a ATP/biosíntesis , Estrógenos no Esteroides/toxicidad , Testículo/efectos de los fármacos , Zearalenona/toxicidad , Envejecimiento/efectos de los fármacos , Envejecimiento/metabolismo , Animales , Animales Recién Nacidos , Western Blotting , Técnicas de Cultivo de Célula , Células Cultivadas , Relación Dosis-Respuesta a Droga , Estradiol/análogos & derivados , Estradiol/farmacología , Antagonistas de Estrógenos/farmacología , Femenino , Fulvestrant , Inmunohistoquímica , Masculino , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células de Sertoli/efectos de los fármacos , Células de Sertoli/metabolismo , Testículo/metabolismoRESUMEN
Different studies have pointed out that developmental exposure to environmental endocrine disruptors can induce long-term testicular germ cell death probably through epigenetic mechanisms. By using a model of early neonatal post-natal day (PND) 1 to 5 exposure of male rats to a xenoestrogen, estradiol benzoate (EB), we investigated the role of microRNA and DNA methyltransferases (DNMT) on the developmental effects of EB on the adult germ cell death process. Neonatal exposure to EB induced adult germ cell apoptosis together with a dose-dependent increase in miR-29a, miR-29b, and miR-29c expression. Increased miR-29 expression resulted in a decrease in DNMT1, DNMT3a, and DNMT3b and antiapoptotic myeloid cell leukemia sequence 1 (Mcl-1) protein levels as shown in 1) germ cells of adult rats exposed neonatally to EB and 2) in spermatogonial GC-1 transfected with miR-29. The DNMT decrease was associated with a concomitant increase in transcript levels of DNA methylation target genes, such as L1td1-1 ORF1 and ORF2, Cdkn2a, and Gstp1, in correlation with their pattern of methylation. Finally, GC-1 cell lines transfection with miR-29a, miR-29b, or miR-29c undergo apoptosis evidenced by Annexin-V expression. Together, the increased miR-29 with a subsequent reduction in DNMT and Mcl-1 protein levels may represent a basis of explanation for the adult expression of the germ cell apoptosis phenotype. These observations suggest that the increased expression of the "apoptomir" miR-29 family represents the upstream mechanism identified until now that is involved in adult germ cell apoptosis induced by a neonatal hormonal disruption.