1.
N Z Med J
; 105(926): 12-3, 1992 Jan 22.
Artículo
en Inglés
| MEDLINE
| ID: mdl-1549267
2.
Anal Biochem
; 143(2): 325-32, 1984 Dec.
Artículo
en Inglés
| MEDLINE
| ID: mdl-6532247
RESUMEN
A method has been developed for extraction and purification of the major bile acids in human feces, and for their quantitative estimation using high-pressure liquid chromatography. Freeze-dried fecal material was extracted with alkaline ethanol and, after removal of neutral steroids, was subjected to thin-layer chromatography, followed by reversed-phase C18 silica cartridge (Sep-Pak) purification. The mixture was further separated into free, and glyco and tauro conjugates by ion-exchange chromatography. Subsequent resolution of individual bile acids was accomplished by HPLC using a counterion pairing method.