Goat and cow milk differ in altering the microbiota composition and neurotransmitter levels in insomnia mouse models.

Milk can improve sleep, and the effects of different animal milks vary. Accordingly, we evaluated the effectiveness of goat milk and cow milk in alleviating insomnia. The findings demonstrated that both goat milk and cow milk significantly increased the length of time that mice with insomnia slept compared to the model group and lowered the relative abundance of Colidextribacter, Escherichia-Shigella, and Proteus in these mice. A notable finding was that goat milk considerably increased the relative abundance of Dubosiella, Bifidobacterium, Lactobacillus, and Mucispirillum, whereas cow milk dramatically increased the relative abundance of Lactobacillus and Acinetobacter. Diazepam therapy could lengthen the slumber of mice; however, analysis of bacteria indicated that although the relative abundance of dangerous bacteria such as Mucispirillum, Parasutterella, Helicobacter, and Romboutsia increased, that of Blautia and Faecalibaculum decreased. Both Listeria and Clostridium experienced a large increase in relative abundance. Additionally, goat milk provided efficient restoration of neurotransmitters including 5-HT, GABA, DA, and NE. Besides that, the expression of genes and proteins for CREB, BDNF, and TrkB in the hypothalamus was up-regulated, and the pathophysiology of the hypothalamus was improved. Overall, the effects of goat and cow milk on insomnia in mouse models differed, and goat milk is preferred over cow milk.

Health Effects of Dietary Oxidized Milk Administration in Offspring Mice during Pregnancy and Lactation with Metabolomic Strategies.

Milk is an important source of nutrients during pregnancy. Previous studies have consistently shown that oxidation in milk and dairy products can induce oxidative stress, inflammation, and fibrosis in the liver and kidney. However, the mechanism underlying these effects remains largely unexplored. This study aimed to investigate the effects of oxidized milk on fecal metabolism and liver and kidney function of offspring mice. Oxidative modification of milk was performed using H2O2-Cu or heating, causing varying degrees of oxidative damage. Kunming female mice were fed with a H2O2-Cu, heat, or normal control diet until their offspring were 3 weeks old. Feces were collected for the metabolomics study based on mass spectrometry. Forty-two potentially significant metabolic biomarkers were screened, and each group's relative intensity was compared. The results showed that oxidized milk mainly regulated isoleucine metabolism, proline metabolism, and tricarboxylic acid cycle. In addition, the histopathological analysis showed accumulation of protein and lipid oxidation products in the liver and kidney tissues after intake of oxidized milk, which induced oxidative stress, increased the levels of inflammatory factors, and significantly increased the expression of genes and proteins involved in inflammatory pathways. The above results suggest that intake of oxidized milk during gestation may increase the risk of liver and kidney injury in male offspring by interfering with amino acid and energy metabolism, highlighting the potential health risks of oxidized milk in humans.