Surface Subsidence Monitoring of Mining Areas in Hunan Province Based on Sentinel-1A and DS-InSAR.

Monitoring the surface subsidence in mining areas is conducive to the prevention and control of geological disasters, and the prediction and early warning of accidents. Hunan Province is located in South China. The mineral resource reserves are abundant; however, large and medium-sized mines account for a low proportion of the total, and the concentration of mineral resource distribution is low, meaning that traditional mining monitoring struggles to meet the needs of large-scale monitoring of mining areas in the province. The advantages of Interferometric Synthetic Aperture Radar (InSAR) technology in large-scale deformation monitoring were applied to identify and monitor the surface subsidence of coal mining fields in Hunan Province based on a Sentinel-1A dataset of 86 images taken from 2018 to 2020, and the process of developing surface subsidence was inverted by selecting typical mining areas. The results show that there are 14 places of surface subsidence in the study area, and accidents have occurred in 2 mining areas. In addition, the railway passing through the mining area of Zhouyuan Mountain is affected by the surface subsidence, presenting a potential safety hazard.

Instability Monitoring and Numerical Analysis of Typical Coal Mines in Southwest China Based on DS-InSAR.

Most of the coal mines in Southwest China are located in mountainous areas with high vegetation coverage, and most activities are carried out under the mountains. The deformation monitoring and mechanical behavior analysis of the mining area helps reveal the typical mountain deformation and failure mechanism caused by underground mining activities and reduce the risk of mountain collapse in the mining area. In this manuscript, a research method for mountain stability in mining areas is proposed, which combines InSAR deformation monitoring with numerical analysis. Based on the high-precision deformation information obtained by DS-InSAR and the landslide range, a three-dimensional explicit finite difference numerical analysis method was used to reconstruct the landslide model. According to the layout of the coal mining working face, the variation mechanism of overlying stratum stress and the mountain slip in the coal mining process is inverted, and the mechanism of mountain failure and instability in the mining area is analysed. Based on the sentinel data, the experiment performed time series monitoring and inversion analysis of the mountain collapse in Nayong, Guizhou, China. The results show that mining activities a certain distance from the mountain will affect mountain stability, and there are specific mechanisms. From 2015 to 2017, the stress redistribution of overlying strata above the goaf area resulted in dense longitudinal cracks in the landslide body due to coal mining. The mountain is in a continuous damage state, and the supporting force to prevent collapse continues to decrease, resulting in a gradual decrease in landslide stability. Both the time series DS-InSAR monitoring results and numerical simulation results verify the actual occurrence and development of the on-site subsidence.

Molecular cloning and sequence analysis of two carbonic anhydrase in the swimming crab Portunus trituberculatus and its expression in response to salinity and pH stress.

Carbonic anhydrase (CA) is involved in ion transport, acid-base balance and pH regulation by catalyzing the interconversion of CO2 and HCO3(-). In this study, full-length cDNA sequences of two CA isoforms were identified from Portunus trituberculatus. One was Portunus trituberculatus cytoplasmic carbonic anydrase (PtCAc) and the other one was Portunus trituberculatus glycosyl-phosphatidylinositol-linked carbonic anhydrase (PtCAg). The sequence of PtCAc was formed by an ORF of 816 bp, encoding a protein of 30.18 kDa. The PtCAg was constituted by an ORF of 927 bp, encoding a protein of 34.09 kDa. The deduced amino acid sequences of the two CA isoforms were compared to other crustacean' CA sequences. Both of them reflected high conservation of the residues and domains essential to the function of the two enzymes. The tissue expression analysis of PtCAc and PtCAg were detected in gill, muscle, hepatopancreas, hemocytes and gonad. PtCAc and PtCAg gene expressions were studied under salinity and pH challenge. The results showed that when salinity decreased (30 to 20 ppt), the mRNA expression of PtCAc increased significantly at 24 and 48 h, and the highest value appeared at 24h. The mRNA expression of PtCAg had the same situation with PtCAc. However, when salinity increased (30 to 35 ppt), only the mRNA expression of PtCAc increased significantly at 48 h. When pH changed, only the mRNA expression of PtCAc increased significantly at 12h, which was under low pH situation. The mRNA expression of PtCAg increased significantly at 12-48 h, and there was no significant difference of the expression between the pH challenged group and the control group in other experimental time. The results provided the base of understanding CA' function and the underlying mechanism in response to environmental changes in crustaceans.

Molecular cloning, characterization and recombinant expression of crustacean hyperglycemic hormone in white shrimp Litopenaeus vannamei.

Crustacean hyperglycemic hormone (CHH) plays an important role in crustacean. In the present study, a full-length cDNA of CHH was cloned from the eyestalk of Litopenaeus vannamei by RACE approach for the first time. The full-length cDNA of LvCHH was 846 bp, containing a 5' untranslated region (UTR) of 65 bp, a 3' UTR of 436 bp with a canonical polyadenylation signal-sequence AATAA and a poly (A) tail, and an open reading frame (ORF) of 345 bp. The ORF encoded a polypeptide of 114 amino acids including a 24 amino acid signal peptide. The calculated molecular mass of the mature protein (74 amino acids) was 8.76 kDa with an estimated pI of 6.78. The sequence of LvCHH was submitted in NCBI GenBank under the accession number HM748790.2. Phylogenetic analysis revealed that LvCHH was clustered with CHH of other crustaceans. Tissue distribution analysis revealed that the expression of LvCHH mRNA was observed in all tissues but gill, and was highest in heart. Specific primers containing Xho I and BamH I restriction sites respectively, were designed based on the obtained ORF sequence of LvCHH gene and the cloning sites of expression vector pET-32a (+). The recombinant plasmid LvCHH-pET32a, was used to transform Escherichia coli BL21 (DE3). LvCHH was successfully expressed by means of SDS-PAGE and western blot analysis. We detected gill Na(+)/K(+)-ATPase activity after rLvCHH protein injection and found that All the experimental group Na(+)/K(+)-ATPase activity presented peak change among 0-6h, and the peaks of all treated groups occurred in 1 h. 20 and 30 µg/shrimp(-1) groups showed significant increase (P<0.05) in 1h post-injection. L. vannamei were exposed for 96h to hypo- and hyper-salinity challenge. Hypo-salinity caused a significant rise (P<0.05) in the mRNA expression of CHH and gill Na(+)/K(+)-ATPase activity at 12h and 24h respectively, then the CHH mRNA expression declining by 24h, and returned to control group level by 48 h, and the Na(+)/K(+)-ATPase activity tended to be stable after 72 h, and higher than that of control. The hyper-salinity challenge had the same trend at mRNA expression with the hypo-salinity group. The Na(+)/K(+)-ATPase activity had no significant change under the low salinity challenge. All these results indicate that LvCHH is an important hormone involved in the osmosis responses of swimming shrimps, and can provide further information of crustacean osmoregulation physiological mechanism.