Human Excreta and Food Waste of a Typical Rural Area in China: Characteristics and Co-Fermentation.

Human excreta (HE) and food waste (FW) are the primary contaminants in rural regions. Prior to treating these contaminants, mastering their properties is required. In this study, the characteristics of the HE leaving the body and FW leaving the kitchen to the subsequent respective fermentation were studied. Moreover, two kinds of co-fermentation processes for HE and FW were also investigated on the basis of mastering the properties. The results showed that, for a healthy adult, fresh feces, urine, and FW produced were about 163 g/cap/d (57.3 gCOD/cap/d), 1.6 L/cap/d (6.7 gN/cap/d), and 250 g/cap/d (35.0 gCOD/cap/d), respectively. In HE, about 75% of nitrogen and phosphorus were contained in urine. It takes at least three days for crushed FW discharged via water flushing to settle completely, and the COD removal efficiency after precipitation was around 75%. Mixing HE with FW after discharge, i.e., the initial unit of the process was 20% more efficient in fermentation than mixing after the respective pre-fermentation. This paper presents the characteristics of HE and FW and provides the optimized co-fermentation process, which provides technical support for the realization of environmental sanitation in rural areas.

Energy recovery potential in coupling of sanitation and agriculture: Techno-economic analysis on resource-oriented sanitation.

The coupling of sanitation system and agriculture production is essential to mitigate the environmental burden and offset unsustainable fertilizer utilization by employing resource-oriented sanitation. Yet, the economic feasibility and energy recovery potential from domestic waste have rarely been investigated. To assess four scenarios (whether with kitchen waste separation; whether with energy recovery) in the resource-oriented sanitation system, an integrated assessment framework based on energy analysis and techno-economic analysis is employed to investigate the comprehensive sanitation system including both wastewater treatment and solid waste disposal. The results show that energy recovery from human excreta and kitchen waste can offset the energy consumption of the sanitation system and the energy surplus can even be 1067.70 kJ·PE-1·day-1. The optimum covering range of the regional recovery center was quantified from the balance between scale effect and spatial distribution, and the serving inhabitants need to be more than 2800.

A dual-response fluorescent probe for the discrimination of cysteine from glutathione and homocysteine.

A highly selective and sensitive turn-on fluorescent BODIPY-based probe for the simultaneous and selective detection of Cys and Hcy/GSH from dual emission channels was developed. The spatial steric hindrance of the methyl groups at 1- and 7-positions in BODIPY skeleton prevented intramolecular displacement of sulfur with amino group of Hcy but not of Cys. GSH molecular skeleton is larger and amino is far away from sulfydryl group, and the product of the reaction of probe with GSH canstay in thiol phase. Therefore, the probe was successfully applied to the detection of Cys from GSH/Hcy. The confocal microscopy experiments implied that this probe is a promising candidate for imaging of Cys and Hcy/GSH in Hela cells.

A water-soluble BODIPY-based fluorescent probe for rapid and selective detection of hypochlorous acid in living cells.

We designed and synthesized 4,4-di-(4'-methylmercaptophenoxy)-8-(N-methylpyridinium-2-yl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (probe 1) as a water-soluble BODIPY derivative for rapid and selective detection of hypochlorous acid. The pyridinium-2-yl linked at the meso position of BODIPY core was used to maintain highly fluorescent nature and to increase water solubility. Methylmercaptophenoxy was selected as responsive site installed on the boron atom (to replace the fluorine atom) and induced the photoinduced electron-transfer (PeT) effect to quench the fluorescence of BODIPY. The probe exhibited 83.9 µg mL-1 solubility in PBS (10 mM, pH 7.4), and possessed very low fluorescence (Φf = 0.0013). Upon addition of HClO, the probe could display a distinct response in 1 min and generate marked fluorescence enhancement by 100-fold due to the oxidation of thioether into sulfoxide to terminate PeT process. A limit of detection of 53 nM was calculated for HClO in the linear response range from 0 µM to 10 µM, and the probe was successfully applied to image HClO in living cells.