RESUMEN
The objective was to determine the effects of dietary net energy (NE) during the grow-finish period on live performance and carcass characteristics of intact male pigs managed with immunological castration (Improvest) compared with physically castrated (PC) male pigs. The 101-d study began when 1,008 pigs (504 intact male pigs and 504 PC male pigs; 10 wk old) were allocated by weight to 48 pens with 21 intact males or 21 PC males per pen. Three dietary NE treatments were fed to pigs using five dietary phases (dietary programs were formulated according to standardized ileal digestible lysine requirements of Improvest males or PC males) to provide an average of 2,212 kcal/kg (low NE), 2,337 kcal/kg (medium NE), or 2,462 kcal/kg (high NE). The experiment was designed and analyzed as a 2â ×â 3 factorial with main effects of Improvest management and NE. For the overall study period, there were no significant interactions between Improvest management and NE (Pâ ≥â 0.19) for average daily feed intake (ADFI), average daily gain (ADG), or gain:feed (G:F). There were also no significant interactions between Improvest management and NE (Pâ ≥â 0.06) for carcass characteristics. Improvest males consumed less feed (5.3% lower ADFI; Pâ <â 0.01), grew faster (5.1% greater ADG; Pâ <â 0.01), and were more efficient (11.2% greater G:F; Pâ <â 0.01) compared with PC males. Hot carcass weight (HCW) did not differ (Pâ =â 0.16) between Improvest males and PC males (attributed to 1.6 percentage unit lower dressing percentage for Improvest males; Pâ <â 0.01); however, Improvest males were leaner (0.9 mm less backfat and 0.65% greater predicted lean yield; Pâ <â 0.01) compared with PC males. For the overall study period, pigs fed low NE and medium NE diets consumed 7.5% and 4.6% more feed (Pâ <â 0.01) than pigs fed high NE diets, respectively, and pigs fed low NE diets grew 1.7% slower (Pâ <â 0.02) than pigs fed medium NE and high NE diets. This resulted in pigs fed low NE diets having 4.4% lower G:F compared with pigs fed medium NE and 8.6% lower G:F compared with pigs fed high NE diets (Pâ <â 0.01). Pigs fed low NE had 3.0 kg lighter (Pâ <â 0.01) HCW compared with medium NE, while high NE had intermediate HCW that did not differ from the other two treatments. Overall, typical Improvest response levels for live performance and carcass characteristics when compared with PC males were achieved for each of the NE treatments evaluated in this study.
RESUMEN
The objective was to determine the effects of net energy (NE) during the grow-finish period on live performance and carcass characteristics of market gilts managed with immunological suppression of ovarian function and estrus (Improvest®; IMP) compared with market gilts not managed with Improvest (CON). The 104-d study began when 1,008 gilts (11 wk old; average starting weight of 30.8 kg) were allocated by weight to 48 pens with 21 gilts/pen. Half of the pens were randomly selected to be managed with Improvest while the other half of the pens were not managed with Improvest. Three dietary programs differing in their NE were formulated over five dietary phases (according to standardized ileal digestible lysine requirements) to provide an average of 2,218 kcal/kg (low NE), 2,343 kcal/kg (medium NE), or 2,468 kcal/kg (high NE). The experiment was designed as a 2â ×â 3 factorial with main effects of Improvest management and NE. For the overall study period, there were no significant interactions (Pâ ≥â 0.20) for average daily feed intake (ADFI), average daily gain (ADG), or Gain:Feed (G:F). There were also no significant interactions between Improvest management and NE (Pâ ≥â 0.30) for carcass characteristics. However, IMP gilts consumed more feed (6.8% greater ADFI; Pâ <â 0.01), grew faster (5.0% greater ADG; Pâ <â 0.01), were less efficient (1.8% lower G:F; Pâ <â 0.01), heavier (3.5 kg hot carcass weight; Pâ <â 0.01), and fatter (1.9 mm greater backfat thickness and 1.26% less predicted lean carcass yield; Pâ <â 0.01). No difference (Pâ =â 0.21) in carcass dressing percentage between IMP and CON gilts was reported. For the overall study period, gilts fed low NE and medium NE diets consumed more feed compared with gilts fed high NE diets (6.8% more ADFI for low NE and 5.7% more for medium NE; Pâ <â 0.01), and gilts fed low NE diets grew 2.5% slower (Pâ <â 0.01) than gilts fed medium NE diets, while gilts fed high NE diets were intermediate and not different from the other NE treatments. This resulted in gilts fed Low NE diets being the least efficient (3.8% lower G:F than medium NE and 7.1% lower G:F than High NE; Pâ <â 0.01). Overall, these data indicate that typical Improvest response levels were sustained at each of the NE treatments evaluated in this study as there were no significant interactions for Improvest management and NE; however, consideration should still be provided to the known production impacts of low NE diets.
RESUMEN
Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease caused by a positive RNA strand arterivirus. PRRS virus (PRRSV) interacts primarily with lung macrophages. Little is known how the virus subverts the innate immune response to initiate its replication in alveolar macrophages. Large-scale transcriptional responses of macrophages with different levels of susceptibility to PRRSV infection were compared over 30 h of infection. This study demonstrates a rapid and intense host transcriptional remodelling during the early phase of the replication of the virus which correlates with transient repression of type-I interferon transcript as early as 8 h post-infection. These results support the suggestion from previous studies that host innate immune response inhibits replication of European porcine reproductive and respiratory syndrome virus in macrophages by altering differential regulation of type-I interferon transcriptional response.