Toxicity of Vanadium during Development of Sea Urchin Embryos: Bioaccumulation, Calcium Depletion, ERK Modulation and Cell-Selective Apoptosis.

Vanadium toxicology is a topic of considerable importance as this metal is widely used in industrial and biomedical fields. However, it represents a potential emerging environmental pollutant because wastewater treatment plants do not adequately remove metal compounds that are subsequently released into the environment. Vanadium applications are limited due to its toxicity, so it is urgent to define this aspect. This metal is associated with sea urchin embryo toxicity as it perturbs embryogenesis and skeletogenesis, triggering several stress responses. Here we investigated its bioaccumulation and the correlation with cellular and molecular developmental pathways. We used cytotoxic concentrations of 1 mM and 500 µM to perform quantitative analyses, showing that vanadium accumulation interferes with calcium uptake during sea urchin development and provokes a disruption in the biomineralization process. At the end of the whole treatment, the accumulation of vanadium was about 14 and 8 µg for embryos treated respectively with 1 mM and 500 µM, showing a dose-dependent response. Then, we monitored the cell signaling perturbation, analyzing key molecular markers of cell survival/cell death mechanisms and the DNA fragmentation associated with apoptosis. This paper clarifies vanadium's trend to accumulate directly into embryonic cells, interfering with calcium uptake. In addition, our results indicate that vanadium can modulate the ERK pathway and activate a cell-selective apoptosis. These results endorse the sea urchin embryo as an adequate experimental model to study metal-related cellular/molecular responses.

Metal Release from Microplastics to Soil: Effects on Soil Enzymatic Activities and Spinach Production.

Microplastics (MPs) represent emergent pollutants in terrestrial ecosystems. Microplastics can cause the release of metal and damage to crop quality. The present research aimed to evaluate the effects of Mater-bi (Bio-MPs) and polyethylene (PE-MPs) MPs at different concentrations on soil properties and on the growth of Spinacia oleracea L. Plants were grown in 30 pots filled with soil mixed with 0.5, 1 and 2% d.w. of Bio-MPs and PE-MPs and in 5 pots filled only with soil, considered as controls (K). At the end of the vegetative cycle, the spinach plants were evaluated for the epigeal (EPI) and hypogeal (HYPO) biomasses and the ratio of HYPO/EPI was calculated. In the soil, the total and the available fractions of Cr, Cu, Ni and Pb and the hydrolase (HA), ß-glucosidase (ß-glu), dehydrogenase (DHA) and urease (U) activities were evaluated. The results revealed that the addition of Bio-MPs increased soil total Cr, Cu and Pb and available Cu concentrations, and the addition of PE-MPs increased Pb availability. In soil contaminated by both Bio-MPs and PE-MPs, HA and ß-glu activities were stimulated, whereas DHA activity was reduced. The HYPO and HYPO/EPI biomasses were reduced only in soils contaminated by the 2% Bio-MPs.

Does the Element Availability Change in Soils Exposed to Bioplastics and Plastics for Six Months?

Plastic sheets are widely used in farming soil to improve the productivity of cultures. Due to their absorption capacity, plastic sheets can alter element and metal content in soils, and in turn affect soil properties. The use of biodegradable films is an attractive eco-sustainable alternative approach to overcome the environmental pollution problems due to the use of plastic films but their impacts on soil are scarcely studied. The aim of the research was to evaluate the impact of conventional plastic and bioplastic sheets on total and available concentrations of elements (Al, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn) in soils. The research was performed in mesocosm trials, filled with soil covered by conventional plastic and bioplastic sheets. After six months of exposure, soils were characterized for pH, water content, concentrations of organic and total carbon and total nitrogen, and total and available Al, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn element concentrations. The results highlighted that soils covered by bioplastic sheets showed higher total and available concentrations of elements and higher contamination factors, suggesting that bioplastic sheets represented a source of metals or a less-effective sink to these background metals in soils, compared to conventional plastic ones.

Soil Biological Responses under Different Vegetation Types in Mediterranean Area.

The knowledge of the effects of fire on soil properties is of particular concern in Mediterranean areas, where the effects of vegetation type are still scarce also. This research aimed: to assess the properties of burnt soils under different vegetation types; to highlight the soil abiotic properties driving the soil microbial biomass and activity under each vegetation type; to compare the biological response in unburnt and burnt soils under the same vegetation type, and between unburnt and burnt soils under different vegetation types. The soils were collected at a Mediterranean area where a large wildfire caused a 50% loss of the previous vegetation types (holm oak: HO, pine: P, black locust: BL, and herbs: H), and were characterized by abiotic (pH, water, and organic matter contents; N concentrations; and C/N ratios) and biotic (microbial and fungal biomasses, microbial respiration, soil metabolic quotient, and hydrolase and dehydrogenase activities) properties. The biological response was evaluated by the Integrative Biological Responses (IBR) index. Before the fire, organic matter and N contents were significantly higher in P than H soils. After the fire, significant increases of pH, organic matter, C/N ratio, microbial biomass and respiration, and hydrolase and dehydrogenase activities were observed in all the soils, especially under HO. In conclusion, the post-fire soil conditions were less favorable for microorganisms, as the IBR index decreased when compared to the pre-fire conditions.

Impact of Anthropic Activities on Soil Quality under Different Land Uses.

Anthropization often leads to land use transformation, causing deep changes to soil properties and its quality. Land use change could be an environmental and socioeconomic problem, as it impacts soil quality and ecosystem services. There is an urgent need to understand the pressures affecting soil quality. The aim of the work is to quantify the impact of different land uses on soil abiotic and biotic properties and on its quality. To achieve the aims, soils from different land uses (forest, urban and agricultural) were collected in the surroundings of Naples and analyzed for pH, water content, contents of C and N, C/N ratio and total and available concentrations of Cu, Ni and Pb, microbial and fungal biomasses, basal respiration and metabolic quotient. Then, a soil quality index (SQI) was calculated for each land use. The results showed that soil abiotic and biotic properties of the agricultural sites differed from those of forest and urban sites. At agricultural sites, microbial abundances decreased due to low amount of C and N and to high amount of Cu and Pb. This caused low use efficiency of energetic substrates and a reduced soil quality of agricultural sites as compared to forest and urban sites.

Combined Effects of Wildfire and Vegetation Cover Type on Volcanic Soil (Functions and Properties) in A Mediterranean Region: Comparison of Two Soil Quality Indices.

Mediterranean regions are the most impacted by fire in Europe. The effects of fire on soil greatly vary according to several factors such as vegetation cover type, but they are scarcely studied. Therefore, this research aimed at evaluating the combined impacts of fire and vegetation on single soil characteristics and on the overall soil quality and functionality through two soil quality indices, simple additive (SQI) and a weighted function (SQIFUNCT). In order to reach the aims, burnt and unburnt soils were collected under different vegetation cover types (herbs and shrubs, black locust, pine and holm oak) within the Vesuvius National Park. The soils were analyzed for the main abiotic (water and organic matter content, total C, N, Ca, K, Cu and Pb concentrations, C/N ratio) and biotic (microbial and fungal biomasses, basal respiration, ß-glucosidase activity) characteristics. On the basis of the investigated soil characteristics, several soil functions (water retention, nutrient supply, contamination content, microorganism habitat and activities), and the soil quality indices were calculated. The results showed that the impact of fire on soil quality and functionality was mediated by the vegetation cover type. In fact, fire occurrence led to a decrease in water and C/N ratio under herbs, a decrease in C concentration under holm oak and a decrease in Cu and Pb concentrations under pine. Although the soil characteristics showed significant changes according to vegetation cover types and fire occurrence, both the additive and weighted function soil quality indices did not significantly vary according to both fire occurrence and the vegetation cover type. Among the different vegetation cover types, pine was the most impacted one.

Do Wildfires Cause Changes in Soil Quality in the Short Term?

Wildfires have high frequency and intensity in the Mediterranean ecosystems that deeply modify the soil abiotic (i.e., pH, contents of water, organic matter and elements) and biotic properties (i.e., biomass and activity). In 2017, an intense wildfire occurred inside the Vesuvius National Park (Southern Italy), destroying approximately 50% of the existing plant cover. So, the research aimed to evaluate the fire effects on soil quality through single soil abiotic and biotic indicators and through an integrated index (SQI). To achieve the aim, soil samples were collected inside the Vesuvius National Park at 12 sampling field points before fire (BF) and after fire (AF). The findings highlighted that in AF soil, the contents of water and total carbon, element availability, respiration and the dehydrogenase activity were lower than in BF soil; in contrast, pH and hydrolase activity were significantly higher in AF soil. The microbial biomass and activity were affected by Al, Cr and Cu availability in both BF and AF soils. Despite the variations in each investigated soil abiotic and biotic property that occurred in AF soil, the overall soil quality did not significantly differ as compared to that calculated for the BF soil. The findings provide a contribution to the baseline definition of the properties and quality of burnt soil and highlight the short-term effects of fire on volcanic soil in the Mediterranean area.

Evaluation of tourism impact on soil metal accumulation through single and integrated indices.

Recently, surface soils of remote or protected areas, that receive pollutants from the surroundings or in situ activities through dry and wet deposition, exceed the baseline content of heavy metals. In the last decades, the use of single and integrated indices is a powerful tool to process, analyze and convey information about metal accumulation degree for decision makers to better manage protected areas. Single indices provide information about only one metal, whereas the integrated ones give a holistic evaluation. The aim of the research was to assess the temporal trends of three single (Contamination Factor, Enrichment Factor and Geo-accumulation Index) and two integrated (Pollution Load Index, Nemerow index) indices in order to evaluate if the touristic impact caused soil metal (Cr, Cu, Ni and Pb) accumulation. In autumn 2016 and in spring 2016 and 2017, the surface soils (0-10 cm) were collected at eight sites inside the Vesuvius National Park (Southern Italy), characterized by intense tourism from spring to autumn. The metal concentrations were measured in the soils and used to calculate the indices. In addition, the Ecological Risk Factor was calculated and the phytotoxicological assays were performed. The findings showed that the surface soils of the Vesuvius National Park were polluted by Cr, Cu, Ni and Pb, according to both single and integrated indices. The touristic impact would seem to be the main cause of soil metal accumulation, as the highest values of the calculated indices were detected for samples collected at the end of the touristic season and the lowest at the beginning of the touristic season. Anyway, Cu and Pb would seem also to derive by ex situ anthropogenic sources, whereas Cr and Ni also by natural sources, such as spontaneous fires and substrate weathering. Finally, the soils showed phytotoxic effects and low ecological risks.

Quercus ilex L. leaves as filters of air Cd, Cr, Cu, Ni and Pb.

The study of particulate matter pollution in urban areas is of great concern due its adverse effects on human health. Roadside vegetation, intercepting and filtering it, contributes to improve air quality. The aim of the research was to investigate the capability of Q. ilex leaves, already known good biomonitors of air quality, in filtering air metals. Besides, the main derivation (i.e. air or root uptake) of the investigated metals in leaf tissue was evaluated. The concentrations of Cd, Cr, Cu, Ni and Pb were measured in three groups of Q. ilex leaves (unwashed, water washed and chloroform washed). Besides, several leaf traits (i.e. length, width, petiole length, leaf area, leaf mass area and thickness) were evaluated. The findings highlighted that Cd, Cr, Cu, Ni and Pb in Q. ilex leaves exceeded the chemical fingerprint. In particular, Cd and Pb appeared the main contaminants of the investigated area as also after water washing of the leaves, their concentrations exceeded the chemical fingerprint. Ni, Cr and Pb appeared to be accumulated on leaf deposit; whereas, Cd appeared mainly adsorbed to leaf cuticle. Higher leaf width, lower leaf area and shorter petiole favoured leaf metal accumulation. Root uptake and translocation to leaves cannot be excluded for Cr and Ni; whereas, leaf Cu content would seem to depend on both leaf deposit and soil content. In conclusion, Q. ilex can be useful in filtering air metal pollution, especially for Ni, Cd, Cr and Pb, and improving air quality.

Overall plant responses to Cd and Pb metal stress in maize: Growth pattern, ultrastructure, and photosynthetic activity.

This study provides a full description of the responses of the crop energy plant Zea mays to stress induced by Cd and Pb, in view of a possible extensive use in phytoattenuation of metal-polluted soils. In this perspective, (i) the uptake capability in root and shoot, (ii) the changes in growth pattern and cytological traits, and (iii) the photosynthetic efficiency based on photochemistry and the level of key proteins were investigated in hydroponic cultures. Both metals were uptaken by maize, with a translocation factor higher for Cd than Pb, but only Cd-treated plants showed a reduced growth compared to control (i.e., a lower leaf number and a reduced plant height), with a biomass loss up to 40%, at the highest concentration of metal (10-3 M). The observation of cytological traits highlighted ultrastructural damages in the chloroplasts of Cd-treated plants. A decline of Rubisco and D1 was observed in plants under Cd stress, while a relevant increase of the same proteins was found in Pb-treated plants, along with an increase of chlorophyll content. Fluorescent emission measurements indicated that both metals induced an increase of NPQ, but only Cd at the highest concentration determined a significant decline of Fv/Fm. These results indicate a different response of Z. mays to individual metals, with Pb triggering a compensative response and Cd inducing severe morpho-physiological alterations at all investigated levels. Therefore, Z. mays could be successfully exploited in phytoattenuation of Pb-polluted soil, but only at very low concentrations of Cd to avoid severe plant damages and biomass loss.

Soil element fractions affect phytotoxicity, microbial biomass and activity in volcanic areas.

Soil quality is strongly affected by microbial biomass that is involved in organic matter mineralization and the supply of nutrients to plants. The effects of trace elements on soil microbial biomass and activity are still controversial, and the contents of the elements in different forms, more than the total amounts, may affect soil microbial community. Volcanic soils are peculiar environments because of their chemical characteristics. Therefore, the aims of this research were to evaluate in volcanic soils: i) the elemental composition; ii) the elemental availability; and iii) the effects of elemental fractions on soil microbial biomass and activity. In order to reach the aims, the BCR sequential extraction method was applied in order to separate 22 elements in different soil fractions: acid soluble, reducible, oxidisable and residual. The studied biological parameters were: microbial and fungal biomasses, soil respiration, metabolic quotient, coefficient of endogenous mineralization, dehydrogenase and hydrolase activities, and phytotoxicity. Among the investigated elements, Al, B, Ba, Ca, Cr, Fe, K, Mg, Mn, Na, Ni, Ti, V and Zn were scarcely available; Cd appeared to be the most ready available element; Zn was mainly present in the acid soluble and in the residual fractions. Microbial biomass and activity appeared to be mainly affected by the reducible and oxidasable fractions of the investigated elements more than the acid soluble or residual ones. With the exception of La and V, the elemental content in the various fractions would seem to stimulate the microbial biomass and activity. Finally, the investigated volcanic soils showed phytotoxic properties.

Total and fraction content of elements in volcanic soil: Natural or anthropogenic derivation.

Soil element composition derives from parent material disaggregation during pedogenesis and weathering processes but also by anthropogenic inputs. Elements are present in soils in different chemical forms that affect their availability and mobility. The aim of the study was to evaluate the main derivation, natural or anthropogenic, of elements in the soils of the Vesuvius National Park (a natural environment strongly affected by human impacts). Besides, the effects of age of the lava from which soils derive, different vegetation covers, traffic fluxes along the two roads connecting the Vesuvius crater and altitudes of the sites on the pseudo-total element concentrations and on their contents in different fraction of soil were investigated. To reach the aims, BCR (Bureau Commun de Référence) sequential extraction was performed in order to determine the distribution of elements into: acid-soluble, reducible, oxidizable and residual fractions. The relationship between the main environmental media and distribution of elements was discussed using non-metric multidimensional scaling (NMDS). The findings showed that, with the exception of Cd, Cu, Pb and Zn that would seem to derive also from human activities, the other investigated elements (Al, As, B, Ba, Ca, Cd, Cr, Cu, Fe, K, La, Mg, Mn, Na, Ni, P, Pb, Si, Ti, V, W and Zn) mainly had a natural derivation. Among the investigated elements, only Cd could represent a potential high risk for the studied andosols. The highest element accumulations in the soils at low altitude could be attributable to an integrated effect of plant cover, vicinity of downtowns and traffic flux. The acid-soluble fraction of elements appeared more linked to lava age; the reducible and oxidizable ones to plant cover; the residual one to the chemical composition of the parent material that gave origin to the soils.