Solid slow-release carbon source assembled microbial fuel cell for promoting superior nitrogen removal in an aerobic granular sludge bioreactor.

Although the coupling process of microbial fuel cell (MFC) and activated sludge is widely used for organic matter removal and electric energy recovery, the problem of high effluent nitrate still exists due to the lack of influent carbon source. Herein, a poly (butanediol succinate) (PBS) assembled MFC was established in an aerobic granular sludge (AGS) bioreactor for simultaneous promoting nitrogen removal and electricity generation. Compared to AGS-Control group, the total inorganic nitrogen (TIN) and COD removal efficiencies of AGS-MFC group were improved to 84.3 ± 2.6% and 93.5 ± 0.5% after 100-days operation. The average output voltage and the maximum power density of the MFC module were 223.7 mV and 59.6 mW/m2, respectively. Through high-throughput sequencing analysis, Thauera-related denitrifying bacteria had the highest relative abundances (20.0% and 31.4%) in both bioreactors. The relative abundance of Nitrosomonas-related ammonia oxidizing bacteria (AOB) in AGS-MFC (1.8%) was enriched than AGS-Control (1.1%). In MFC module, Thauera (16.2%) with denitrification and power generation was dominant in anodic biofilms under PBS enhancement. This study provides scientific basis for the application of submersible MFC enhanced deep nitrogen removal under aerobic conditions.

Microbial fuel cell enhanced pollutants removal in a solid-phase biological denitrification reactor: System performance, bioelectricity generation and microbial community analysis.

A novel electrochemical system of microbial fuel cell (MFC) coupled solid-phase denitrification biofilm reactor (DBR) system was established to explore the effect of simultaneous power generation and pollutant removal under different HRTs (Ⅰ:48 h; Ⅱ :24 h). The average removal rates of methyl orange, Cr (VI) and NO3--N in test group were 93.0, 98.6 and 95.5% within 60 days, while those were 53.1, 72.1 and 72.7% in control. The maximum power density was 61.2 (Ⅰ) and 16.1 mW/m2 (Ⅱ), while average output voltage was 122 (Ⅰ) and 83.6 mV (Ⅱ). Components 1 and 2 in soluble microbial products were identified, and the humic-like and fulvic acid-like substances varied through different layers. Pseudomonas produced electricity in anode, while denitrified in denitrification layer. Importantly, symbiotic cooperation was absolutely dominant in network analysis of both anodic and denitrifying biofilms. MFC significantly improved DBR's ability to treatment co-polluted wastewater.

Coupling of nitrifying granular sludge into microbial fuel cell system for wastewater treatment: System performance, electricity production and microbial community shift.

A novel electrochemical system coupling of nitrifying granular sludge (NGS) into microbial fuel cell (MFC) system was conducted for simultaneous electricity production and wastewater treatment under sequencing batch mode. After 60 days operation, the contaminants of organic and NH4+-N removal efficiencies of the system were high of 95.43% and 98.55%, respectively. The maximum output voltage and power density of the MFC were average at 170 mV and 33.24 mW/m2, respectively. According to EEM-PARAFAC model, the soluble microbial products (SMP) released from anode and cathode chambers could be identified two fluorescence components. Additionally, the fluorescence score of protein-like substances changed more obvious than those of humic-like and fulvic acid-like substances. Geobacter and Nitrospiraceae were the dominant functional populations in the anode and cathode chambers, respectively. The result could provide a potential application technology based on NGS-MFC for simultaneously treatment of organic matter and ammonia.