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N Engl J Med ; 370(25): 2408-17, 2014 Jun 19.
Artículo en Inglés | MEDLINE | ID: mdl-24896819

RESUMEN

A 14-year-old boy with severe combined immunodeficiency presented three times to a medical facility over a period of 4 months with fever and headache that progressed to hydrocephalus and status epilepticus necessitating a medically induced coma. Diagnostic workup including brain biopsy was unrevealing. Unbiased next-generation sequencing of the cerebrospinal fluid identified 475 of 3,063,784 sequence reads (0.016%) corresponding to leptospira infection. Clinical assays for leptospirosis were negative. Targeted antimicrobial agents were administered, and the patient was discharged home 32 days later with a status close to his premorbid condition. Polymerase-chain-reaction (PCR) and serologic testing at the Centers for Disease Control and Prevention (CDC) subsequently confirmed evidence of Leptospira santarosai infection.


Asunto(s)
Encéfalo/patología , Líquido Cefalorraquídeo/microbiología , ADN Bacteriano/análisis , Leptospira/genética , Leptospirosis/diagnóstico , Meningoencefalitis/diagnóstico , Análisis de Secuencia de ADN/métodos , Adenosina Desaminasa/deficiencia , Adolescente , Agammaglobulinemia/complicaciones , Biopsia , Fiebre/etiología , Cefalea/etiología , Humanos , Leptospira/aislamiento & purificación , Leptospirosis/complicaciones , Leptospirosis/microbiología , Masculino , Meningoencefalitis/complicaciones , Meningoencefalitis/microbiología , Inmunodeficiencia Combinada Grave/complicaciones
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