Trace elements, dioxins and PCBs in different fish species and marine regions: Importance of the taxon and regional features.

Chemical contaminant concentrations in wild organisms are used to assess environmental status under the European Marine Strategy Framework Directive. However, this approach is challenged by the complex intra- and inter-species variability, and the different regional features. In this study, concentrations in trace elements (As, Cd, Hg and Pb), polychlorinated biphenyls (PCBs), polychlorodibenzo-para-dioxines (PCDDs) and polychlorodibenzofuranes (PCDFs) were monitored in 8 fish species sampled on the continental shelf of three French regions: the Eastern English Channel (EEC) and Bay of Biscay (BoB) in the Northeast Atlantic Ocean, and the Gulf of Lions (GoL) in Western Mediterranean Sea. Our objectives were to identify species or regions more likely to be contaminated and to assess how to take this variability into account in environmental assessment. While concentrations were higher in benthic and demersal piscivores, PCB and PCDD/F concentrations (lipid-weight) were similar in most teleost species. For Cd, Hg and Pb, the trophic group accumulating the highest concentrations depended on the contaminant and region. Concentrations in Hg, PCBs and PCDD/Fs were higher in the EEC and/or GoL than in BoB. Cadmium and Pb concentrations were highest in the BoB. Lipid content accounted for 35%-84% of organic contaminant variability. Lipid normalisation was employed to enhance robustness in the identification of spatial patterns. Contaminant patterns in chondrichthyans clearly differed from that in teleosts. In addition, trophic levels accounted for ≤1% and ≤33% of the contaminant variability in teleost fishes in the EEC and BoB, respectively. Therefore, developing taxa-specific thresholds might be a more practical way forward for environmental assessment than normalisation to trophic levels.

Modeling the impacts of multiple environmental stress factors on estuarine copepod populations.

Many studies have focused on natural stress factors that shape the spatial and temporal distribution of calanoid copepods, but bioassays have shown that copepods are also sensitive to a broad range of contaminants. Although both anthropogenic and natural stress factors are obviously at play in natural copepod communities, most studies consider only one or the other. In the present investigation, we modeled the combined impact of both anthropogenic and natural stress factors on copepod populations. The model was applied to estimate Eurytemora affinis densities in the contaminated Scheldt estuary and the relatively uncontaminated Darß-Zingst estuary in relation to temperature, salinity, chlorophyll a, and sediment concentrations of cadmium, copper, and zinc. The results indicated that temperature was largely responsible for seasonal fluctuations of E. affinis densities. Our model results further suggested that exposure to zinc and copper was largely responsible for the reduced population densities in the contaminated estuary. The model provides a consistent framework for integrating and quantifying the impacts of multiple anthropogenic and natural stress factors on copepod populations. It facilitates the extrapolation of laboratory experiments to ecologically relevant end points pertaining to population viability.

Selective feeding of bdelloid rotifers in river biofilms.

In situ pigment contents of biofilm-dwelling bdelloid rotifers of the Garonne River (France) were measured by high performance liquid chromatography (HPLC) and compared with pigment composition of surrounding biofilm microphytobenthic communities. Among pigments that were detected in rotifers, the presence of carotenoids fucoxanthin and myxoxanthophyll showed that the rotifers fed on diatoms and cyanobacteria. Unexpectedly, while diatoms strongly dominated microphytobenthic communities in terms of biomass, HPLC results hinted that rotifers selectively ingested benthic filamentous cyanobacteria. In doing so, rotifers could daily remove a substantial fraction (up to 28%) of this cyanobacterial biomass. The possibility that the rotifers hosted symbiotic myxoxanthophyll-containing cyanobacteria was examined by localisation of chlorophyll fluorescence within rotifers using confocal laser scanning microscopy (CLSM). CLSM results showed an even distribution of quasi-circular fluorescent objects (FO) throughout rotifer bodies, whereas myxoxanthophyll is a biomarker pigment of filamentous cyanobacteria, so the hypothesis was rejected. Our results also suggest that rotifers converted ß-carotene (provided by ingested algae) into echinenone, a photoprotective pigment. This study, which is the first one to detail in situ pigment contents of rotifers, clearly shows that the role of cyanobacteria as a food source for meiobenthic invertebrates has been underestimated so far, and deserves urgent consideration.