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1.
Korean J Physiol Pharmacol ; 22(5): 577-584, 2018 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-30181704

RESUMEN

Bladder dysfunction is a common complication of diabetes mellitus (DM). However, there have been a few studies evaluating bladder smooth muscle contraction in DM in the presence of pharmacological inhibitors. In the present study, we compared the contractility of bladder smooth muscle from normal rats and DM rats. Furthermore, we utilized pharmacological inhibitors to delineate the mechanisms underlying bladder muscle differences between normal and DM rats. DM was established in 14 days after using a single injection of streptozotocin (65 mg/kg, intraperitoneal) in Sprague-Dawley rats. Bladder smooth muscle contraction was induced electrically using electrical field stimulation consisting of pulse trains at an amplitude of 40 V and pulse duration of 1 ms at frequencies of 2-10 Hz. In this study, the pharmacological inhibitors atropine (muscarinic receptor antagonist), U73122 (phospholipase C inhibitor), DPCPX (adenosine A1 receptor antagonist), udenafil (PDE5 inhibitor), prazosin (α1-receptor antagonist), verapamil (calcium channel blocker), and chelerythrine (protein kinase C inhibitor) were used to pretreat bladder smooth muscles. It was found that the contractility of bladder smooth muscles from DM rats was lower than that of normal rats. In addition, there were significant differences in percent change of contractility between normal and DM rats following pretreatment with prazosin, udenafil, verapamil, and U73122. In conclusion, we suggest that the decreased bladder muscle contractility in DM rats was a result of perturbations in PLC/IP3-mediated intracellular Ca2+ release and PDE5 activity.

2.
Korean J Physiol Pharmacol ; 20(6): 595-603, 2016 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-27847436

RESUMEN

Ribosomal S6 kinase is a family of serine/threonine protein kinases involved in the regulation of cell viability. There are two subfamilies of ribosomal s6 kinase, (p90rsk, p70rsk). Especially, p90rsk is known to be an important downstream kinase of p44/42 MAPK. We investigated the role of p90rsk on ethanol-induced cell proliferation of HepG2 cells. HepG2 cells were treated with 10~50 mM of ethanol with or without ERK and p90rsk inhibitors. Cell viability was measured by MTT assay. The expression of pERK1, NHE1 was measured by Western blots. The phosphorylation of p90rsk was measured by ELISA kits. The expression of Bcl-2 was measured by qRT-PCR. When the cells were treated with 10~30 mM of ethanol for 24 hour, it showed significant increase in cell viability versus control group. Besides, 10~30 mM of ethanol induced increased expression of pERK1, p-p90rsk, NHE1 and Bcl-2. Moreover treatment of p90rsk inhibitor attenuated the ethanol-induced increase in cell viability and NHE1 and Bcl-2 expression. In summary, these results suggest that p90rsk, a downstream kinase of ERK, plays a stimulatory role on ethanol-induced hepatocellular carcinoma progression by activating anti-apoptotic factor Bcl-2 and NHE1 known to regulate cell survival.

3.
Pharmazie ; 69(3): 224-8, 2014 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-24716414

RESUMEN

The present study was undertaken to investigate the influence of vitexin on vascular smooth muscle contractility and to determine the mechanism involved. Intact or denuded aortic rings from male rats were used and isometric contractions were recorded and combined with molecular experiments. Vitexin more significantly relaxed phorbol ester-induced vascular contraction than thromboxane A2 or fluoride-induced contraction suggesting as a possible anti-hypertensive on the agonist-induced vascular contraction regardless of endothelial nitric oxide synthesis. Furthermore, vitexin significantly inhibited phorbol ester-induced increases in pERK1/2 levels. On the other hand, it did not significantly inhibit thromboxane A2-induced increases in pMYPT1 levels suggesting the mechanism involving the primarily inhibition of MEK activity and the subsequent phosphorylation of ERK1/2. This study provides evidence regarding the mechanism underlying the relaxation effect of vitexin on agonist-induced vascular contraction regardless of endothelial function.


Asunto(s)
Apigenina/farmacología , Músculo Liso Vascular/efectos de los fármacos , Animales , Aorta Torácica/efectos de los fármacos , Western Blotting , Activadores de Enzimas/farmacología , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Masculino , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Contracción Muscular/efectos de los fármacos , Ésteres del Forbol/farmacología , Proteína Fosfatasa 1/metabolismo , Ratas , Ratas Sprague-Dawley , Fluoruro de Sodio/farmacología , Tromboxano A2/farmacología , Quinasas Asociadas a rho/metabolismo
4.
Korean J Physiol Pharmacol ; 18(3): 249-54, 2014 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-24976765

RESUMEN

The purpose of this study is to characterize the effects of KHG26792 (3-(naphthalen-2-yl(propoxy) methyl)azetidine hydrochloride), a potential skin whitening agent, on melanin synthesis and identify the underlying mechanism of action. Our data showed that KHG26792 significantly reduced melanin synthesis in a dose-dependent manner. Additionally, KHG26792 downregulated microphthalmia-associated transcription factor (MITF) and tyrosinase, the rate-limiting enzyme in melanogenesis, although tyrosinase was not inhibited directly. KHG26792 activated extracellular signal-regulated kinase (ERK), whereas an ERK pathway inhibitor, PD98059, rescued KHG26792-induced hypopigmentation. These results suggest that KHG26792 decreases melanin production via ERK activation. Moreover, the hypopigmentary effects of KHG26792 were confirmed in a pigmented skin equivalent model using Cervi cornus Colla (deer antler glue), in which the color of the pigmented artificial skin became lighter after treatment with KHG26792. In summary, our findings suggest that KHG26792 is a novel skin whitening agent.

5.
Biomol Ther (Seoul) ; 32(3): 361-367, 2024 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-38589300

RESUMEN

In this study, we investigated the efficacy of kaempferol (a flavonoid found in plants and plant-derived foods such as kale, beans, tea, spinach and broccoli) on vascular contractibility and aimed to clarify the detailed mechanism underlying the relaxation. Isometric contractions of divested muscles were stored and linked with western blot analysis which was carried out to estimate the phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and phosphorylation-dependent inhibitory protein for myosin phosphatase (CPI-17) and to estimate the effect of kaempferol on the RhoA/ROCK/CPI-17 pathway. Kaempferol conspicuously impeded phorbol ester-, fluoride- and a thromboxane mimetic-derived contractions regardless of endothelial nitric oxide synthesis, indicating its direct effect on smooth muscles. It also conspicuously impeded the fluoride-derived elevation in phospho-MYPT1 rather than phospho-CPI-17 levels and phorbol 12,13-dibutyrate-derived increase in phospho-CPI-17 and phospho-ERK1/2 levels, suggesting the depression of PKC and MEK activities and subsequent phosphorylation of CPI-17 and ERK1/2. Taken together, these outcomes suggest that kaempferol-derived relaxation incorporates myosin phosphatase retrieval and calcium desensitization, which appear to be modulated by CPI-17 dephosphorylation mainly through PKC inactivation.

6.
Ecol Food Nutr ; 52(3): 223-38, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23621487

RESUMEN

Pigs' feet are traditionally consumed by Korean women to increase milk production during lactation. In this study, we evaluated the perceived effectiveness and safety of consuming pigs' feet. Parous women were recruited to complete survey questionnaires in South Korea. Of the 516 respondents, 188 (36%) claimed they consumed pigs' feet. One-hundred twenty women (64%) who consumed pigs' feet reported experiencing an increase in breast milk production. Seventy-three (61%) women from this same group exclusively breastfed. In contrast, 16 (25%) of 65 respondents who did not embrace the same purported effect of pigs' feet indicated that they solely breastfed. Ninety-eight (82%) respondents who consumed pigs' feet and reported galactagoguic effects would recommend its use. Study findings reveal that the majority of women who consume pigs' feet believe in its milk-promoting effects. The results of this study suggest that further investigation of the galactagoguic effects of pigs' feet via conduction of an experimental study is warranted.


Asunto(s)
Lactancia Materna , Cultura , Conducta Alimentaria , Galactogogos , Lactancia , Carne , Leche Humana , Adolescente , Adulto , Animales , Recolección de Datos , Dieta , Femenino , Humanos , Percepción , República de Corea , Seguridad , Encuestas y Cuestionarios , Porcinos , Adulto Joven
7.
Korean J Physiol Pharmacol ; 17(5): 469-77, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-24227950

RESUMEN

This study investigated effect of extract containing quercetin-3-O-ß-D-glucuronopyranoside from Rumex Aquaticus Herba (ECQ) against chronic gastritis in rats. To produce chronic gastritis, the animals received a daily intra-gastric administration of 0.1 ml of 0.15% iodoacetamide (IA) solution for 7 days. Daily exposure of the gastric mucosa to IA induced both gastric lesions and significant reductions of body weight and food and water intake. These reductions recovered with treatment with ECQ for 7 days. ECQ significantly inhibited the elevation of the malondialdehyde levels and myeloperoxidase activity, which were used as indices of lipid peroxidation and neutrophil infiltration. ECQ recovered the level of glutathione, activity of superoxide dismutase (SOD), and expression of SOD-2. The increased levels of total NO concentration and iNOS expression in the IA-induced chronic gastritis were significantly reduced by treatment with ECQ. These results suggest that the ECQ has a therapeutic effect on chronic gastritis in rats by inhibitory actions on neutrophil infiltration, lipid peroxidation and various steps of reactive oxygen species (ROS) generation.

8.
Biosci Biotechnol Biochem ; 76(4): 767-71, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22484949

RESUMEN

Xanthium strumarium L. (Asteraceae) is traditionally used in Korea to treat skin diseases. In this study, we investigated the effects of a X. strumarium stem extract on melanin synthesis. It inhibited melanin synthesis in a concentration-dependent manner, but it did not directly inhibit tyrosinase, the rate-limiting melanogenic enzyme, and instead downregulated microphthalmia-associated transcription factor (MITF) and tyrosinase expression. MITF, the master regulator of pigmentation, is a target of the Wnt signaling pathway, which includes glycogen synthase kinase 3ß (GSK3ß) and ß-catenin. Hence, the influence of X. strumarium stem extract on GSK3ß and ß-catenin was further investigated. X. strumarium induced GSK3ß phosphorylation (inactivation), but the level of ß-catenin did not change. Moreover, a specific GSK3ß inhibitor restored X. strumarium-induced melanin reduction. Hence, we suggest that X. strumarium inhibits melanin synthesis through downregulation of tyrosinase via GSK3ß phosphorylation.


Asunto(s)
Glucógeno Sintasa Quinasa 3/metabolismo , Melanocitos/efectos de los fármacos , Factor de Transcripción Asociado a Microftalmía/antagonistas & inhibidores , Monofenol Monooxigenasa/antagonistas & inhibidores , Extractos Vegetales/farmacología , Xanthium/química , Animales , Línea Celular Transformada , Relación Dosis-Respuesta a Droga , Regulación hacia Abajo/efectos de los fármacos , Glucógeno Sintasa Quinasa 3/genética , Glucógeno Sintasa Quinasa 3 beta , Melaninas/genética , Melaninas/metabolismo , Melanocitos/citología , Melanocitos/metabolismo , Ratones , Factor de Transcripción Asociado a Microftalmía/genética , Factor de Transcripción Asociado a Microftalmía/metabolismo , Monofenol Monooxigenasa/genética , Monofenol Monooxigenasa/metabolismo , Fosforilación , Pigmentación/genética , Extractos Vegetales/aislamiento & purificación , Tallos de la Planta/química , Vía de Señalización Wnt/efectos de los fármacos , Vía de Señalización Wnt/genética , beta Catenina/genética , beta Catenina/metabolismo
9.
Biomol Ther (Seoul) ; 30(2): 145-150, 2022 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-34231489

RESUMEN

In this study, we investigated the influence of galangin on vascular contractibility and to determine the mechanism underlying the relaxation. Isometric contractions of denuded aortic muscles were recorded and combined with western blot analysis which was performed to measure the phosphorylation of phosphorylation-dependent inhibitory protein of myosin phosphatase (CPI-17) and myosin phosphatase targeting subunit 1 (MYPT1) and to evaluate the effect of galangin on the RhoA/ROCK/CPI-17 pathway. Galangin significantly inhibited phorbol ester-, fluoride- and thromboxane mimetic-induced vasoconstrictions regardless of endothelial nitric oxide synthesis, suggesting its direct effect on vascular smooth muscle. Galangin significantly inhibited the fluoride-dependent increase in pMYPT1 and pCPI-17 levels and phorbol 12,13-dibutyrate-dependent increase in pERK1/2 level, suggesting repression of ROCK and MEK activity and subsequent phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that galangin-induced relaxation involves myosin phosphatase reactivation and calcium desensitization, which appears to be mediated by CPI-17 dephosphorylation via not PKC but ROCK inactivation.

10.
Biomol Ther (Seoul) ; 30(4): 348-359, 2022 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-35768332

RESUMEN

Gastric adenocarcinoma is among the top causes of cancer-related death and is one of the most commonly diagnosed carcinomas worldwide. Benzyl isothiocyanate (BITC) has been reported to inhibit the gastric cancer metastasis. In our previous study, BITC induced apoptosis in AGS cells. The purpose of the present study was to investigate the effect of BITC on autophagy mechanism in AGS cells. First, the AGS cells were treated with 5, 10, or 15 µM BITC for 24 h, followed by an analysis of the autophagy mechanism. The expression level of autophagy proteins involved in different steps of autophagy, such as LC3B, p62/SQSTM1, Atg5-Atg12, Beclin1, p-mTOR/mTOR ratio, and class III PI3K was measured in the BITC-treated cells. Lysosomal function was investigated using cathepsin activity and Bafilomycin A1, an autophagy degradation stage inhibitor. Methods including qPCR, western blotting, and immunocytochemistry were employed to detect the protein expression levels. Acridine orange staining and omnicathepsin assay were conducted to analyze the lysosomal function. siRNA transfection was performed to knock down the LC3B gene. BITC reduced the level of autophagy protein such as Beclin 1, class III PI3K, and Atg5-Atg12. BITC also induced lysosomal dysfunction which was shown as reducing cathepsin activity, protein level of cathepsin, and enlargement of acidic vesicle. Overall, the results showed that the BITC-induced AGS cell death mechanism also comprises the inhibition of the cytoprotective autophagy at both initiation and degradation steps.

11.
J Med Food ; 22(4): 355-364, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30990752

RESUMEN

Our study aimed to investigate the effects of the polysaccharide-rich extract of Phragmites rhizoma (PEP) against water immersion restraint (WIR) stress and forced swimming-induced fatigue. Exposure to WIR stress significantly increased the ulcer index, bleeding score, the weight of the adrenal gland, blood glucose concentrations, total cholesterol, cortisol, and creatine kinase (CK). The weight of the spleen decreased significantly. In addition, myeloperoxidase (MPO) and thiobarbituric acid-reactive substance (TBARS) were significantly upregulated by WIR stress. The antioxidative factors such as glutathione (GSH) and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in the stomach were decreased by WIR stress. Alterations induced by WIR stress were effectively reversed by pretreatment with PEP. The swimming endurance capacity of mice was significantly prolonged by the oral administration of PEP. Swimming-induced fatigue significantly reduced the body weight; however, the injection of PEP inhibited the decrease of body weight. The PEP-treated group had significantly lower CK levels in plasma, an indicator of muscle damage. These results indicated that PEP has anti-stress and anti-fatigue effects, which are mediated by suppressing the hyperactivation of the hypothalamus-pituitary-adrenal axis, and antagonism of the oxidative damages induced by WIR stress and prolonged swimming times.


Asunto(s)
Fatiga/tratamiento farmacológico , Extractos Vegetales/administración & dosificación , Poaceae/química , Polisacáridos/administración & dosificación , Animales , Catalasa/metabolismo , Modelos Animales de Enfermedad , Fatiga/metabolismo , Fatiga/fisiopatología , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Humanos , Masculino , Ratones , Ratones Endogámicos ICR , Peroxidasa/metabolismo , Extractos Vegetales/química , Polisacáridos/química , Rizoma/química , Estrés Fisiológico/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Natación
12.
Arch Pharm Res ; 41(10): 1019-1031, 2018 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-30109575

RESUMEN

Indomethacin is a non-steroidal anti-inflammatory drug with clearly known side effects on the gastrointestinal tract. The purpose of the present study was to investigate whether eupatilin inhibit cell injury induced by indomethacin in cultured feline esophageal epithelial cells (EECs). EECs were used to investigate the ability of eupatilin to induce the expression of heat shock proteins (HSP27 and HSP70) and analyze its cytoprotective effect against indomethacin-induced damage. The treatment of EECs with indomethacin for 8 h decreased cell viability. Western blot analysis showed that the levels of HSPs gradually decreased in cells treated with indomethacin, while eupatilin treatment increased the levels of HSPs. When treated with both indomethacin and eupatilin, the levels of HSPs increased rapidly, and were maintained at 130-140%. In addition, treatment with the specific inhibitors of PTK, PKC, PLC, p38 MAPK, JNKs, and PI3K attenuated the eupatilin-induced expression of HSPs. Pretreatment of EECs with the inhibitors of protein synthesis, actinomycin D or cycloheximide, attenuated the cytoprotective effect of eupatilin on indomethacin-induced cell damage. Reactive oxygen species production was upregulated by indomethacin, but downregulated by eupatilin. Taken together, it was suggested that HSPs were partly responsible for the eupatilin-mediated cytoprotective activity against the indomethacin-induced damage in EECs.


Asunto(s)
Citoprotección/efectos de los fármacos , Células Epiteliales/efectos de los fármacos , Esófago/citología , Flavonoides/farmacología , Proteínas de Choque Térmico HSP27/biosíntesis , Proteínas HSP70 de Choque Térmico/biosíntesis , Indometacina/antagonistas & inhibidores , Animales , Benzofenantridinas/farmacología , Gatos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Estrenos/farmacología , Femenino , Flavonoides/antagonistas & inhibidores , Técnica del Anticuerpo Fluorescente , Proteínas de Choque Térmico HSP27/análisis , Proteínas de Choque Térmico HSP27/antagonistas & inhibidores , Proteínas HSP70 de Choque Térmico/análisis , Proteínas HSP70 de Choque Térmico/antagonistas & inhibidores , Indometacina/farmacología , Masculino , Pirrolidinonas/farmacología , Tirfostinos/farmacología
13.
Arch Pharm Res ; 41(10): 955-966, 2018 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-30132170

RESUMEN

Irritable bowel syndrome (IBS), a common gastrointestinal (GI) disorder, is associated with various factors, including lifestyle, infection, stress, intestinal flora, and related diseases. The pharmacotherapeutic stimulation of receptors and downstream signaling pathways is effective in reducing IBS symptoms; however, it is still associated with adverse effects. Various receptors related to GI motility and visceral hypersensitivity should be considered to enhance the benefit/risk ratio of IBS treatments. This review discusses recent pharmacological advances in IBS management. Several receptors related to GI motility and abdominal pain are investigated in various angles. 5-Hydroxytryptamine (5-HT) is an important neurotransmitter that activates the colonic mucosal 5-HT4 receptor without causing severe cardiovascular adverse effects. The clinical potential of ramosetron for diarrhea-predominant IBS has been suggested because of a lower risk of ischemic colitis than conventional 5-HT3 receptor antagonists. Toll-like receptors (TLRs), especially TLR2 and TLR4, show a significant effect on the post-infection symptoms and lipopolysaccharide-mediated regulation of GI motility. Histamine is a well-known nitrogenous compound that regulates inflammatory responses and visceral hypersensitivity. Histamine 1 receptor-mediated sensitization of the transient receptor potential vanilloid 1 is associated with IBS. Pharmacological approaches based on these signaling pathways could be useful in the development of novel IBS treatments.


Asunto(s)
Síndrome del Colon Irritable/tratamiento farmacológico , Motilidad Gastrointestinal/efectos de los fármacos , Humanos , Síndrome del Colon Irritable/metabolismo , Receptores de Serotonina/metabolismo , Canales Catiónicos TRPV/antagonistas & inhibidores , Canales Catiónicos TRPV/metabolismo
14.
J Med Food ; 21(10): 1009-1015, 2018 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-30334699

RESUMEN

It has been described that Humulus japonicus has potential antituberculosis and anti-inflammatory effects. The antiaging activity of H. japonicus extract (HJE) was also examined not only in yeast but also in human fibroblast cells. We evaluated the protective effect of HJE on hepatotoxicity in this study. We demonstrated the expression of antioxidative enzyme and cytokines in plasma. The vehicle control group received orally administered normal saline. Acute hepatotoxicity rat model was induced by lipopolysaccharide (LPS) (30 µg/kg) and d-galactosamine (D-GalN) (500 mg/kg) by intraperitoneal injection. The positive control group received orally administered silymarin (10 mg/kg). Three HJE groups received 8, 16, and 32 mg/kg. The blood samples were prepared to evaluate malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) level to examine oxidative stress, and hepatic tissue was assayed for myeloperoxidase (MPO). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and tumor necrosis factor-α (TNF-α) activities were also assayed to examine liver cell viability. Pretreatment with HJE decreased levels of AST, ALT, MDA, MPO, and TNF-α and increased levels of SOD and CAT compared with the LPS/D-GalN-treated group. These results suggested that HJE has the potential to reduce oxidative damage and inflammatory reactions in LPS/D-GalN-induced liver injury in the rat model. It can also increase survival rate in LPS/D-GalN-induced hepatotoxicity rat models.


Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & control , Galactosamina/efectos adversos , Humulus/química , Lipopolisacáridos/efectos adversos , Extractos Vegetales/administración & dosificación , Sustancias Protectoras/administración & dosificación , Alanina Transaminasa/genética , Alanina Transaminasa/metabolismo , Animales , Aspartato Aminotransferasas/genética , Aspartato Aminotransferasas/metabolismo , Catalasa/metabolismo , Enfermedad Hepática Inducida por Sustancias y Drogas/enzimología , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Humanos , Hígado/efectos de los fármacos , Hígado/enzimología , Masculino , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo
15.
Biomol Ther (Seoul) ; 26(6): 546-552, 2018 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-29915165

RESUMEN

A comprehensive collection of proteins senses local changes in intracellular Ca²âº concentrations ([Ca²âº]i) and transduces these signals into responses to agonists. In the present study, we examined the effect of sphingosine-1-phosphate (S1P) on modulation of intracellular Ca²âº concentrations in cat esophageal smooth muscle cells. To measure [Ca²âº]i levels in cat esophageal smooth muscle cells, we used a fluorescence microscopy with the Fura-2 loading method. S1P produced a concentration-dependent increase in [Ca²âº]i in the cells. Pretreatment with EGTA, an extracellular Ca²âº chelator, decreased the S1P-induced increase in [Ca²âº]i, and an L-type Ca²âº-channel blocker, nimodipine, decreased the effect of S1P. This indicates that Ca²âº influx may be required for muscle contraction by S1P. When stimulated with thapsigargin, an intracellular calcium chelator, or 2-Aminoethoxydiphenyl borate (2-APB), an InsP3 receptor blocker, the S1P-evoked increase in [Ca²âº]i was significantly decreased. Treatment with pertussis toxin (PTX), an inhibitor of Gi-protein, suppressed the increase in [Ca²âº]i evoked by S1P. These results suggest that the S1P-induced increase in [Ca²âº]i in cat esophageal smooth muscle cells occurs upon the activation of phospholipase C and subsequent release of Ca²âº from the InsP3-sensitive Ca²âº pool in the sarcoplasmic reticulum. These results suggest that S1P utilized extracellular Ca²âº via the L type Ca²âº channel, which was dependent on activation of the S1P4 receptor coupled to PTX-sensitive Gi protein, via phospholipase C-mediated Ca²âº release from the InsP3-sensitive Ca²âº pool in cat esophageal smooth muscle cells.

16.
Biomol Ther (Seoul) ; 26(4): 374-379, 2018 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-29390250

RESUMEN

In this study, we investigated the effects of pelargonidin, an anthocyanidin found in many fruits and vegetables, on endothelium-independent vascular contractility to determine the underlying mechanism of relaxation. Isometric contractions of denuded aortic muscles from male rats were recorded, and the data were combined with those obtained in western blot analysis. Pelargonidin significantly inhibited fluoride-, thromboxane A2-, and phorbol ester-induced vascular contractions, regardless of the presence or absence of endothelium, suggesting a direct effect of the compound on vascular smooth muscles via a different pathway. Pelargonidin significantly inhibited the fluoride-dependent increase in the level of myosin phosphatase target subunit 1 (MYPT1) phosphorylation at Thr-855 and the phorbol 12,13-dibutyrate-dependent increase in the level of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation at Thr202/Tyr204, suggesting the inhibition of Rho-kinase and mitogen-activated protein kinase kinase (MEK) activities and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxation effect of pelargonidin on agonist-dependent vascular contractions includes inhibition of Rho-kinase and MEK activities, independent of the endothelial function.

17.
Arch Pharm Res ; 30(12): 1608-18, 2007 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-18254249

RESUMEN

We investigated the signaling pathway on sphingosinephosphorylcholine (SPC) -induced contraction in cat esophageal smooth muscle cells. SPC induced in a dose-dependent manner contractile effect. We have previously shown that lysophospholipid (LPL) receptor subtypes including the S1P1, S1P2, S1P3, and S1P5 receptor are present in esophageal smooth muscle. Only EDG-5 (S1P2) receptor antibody penetration into permeablilized cells inhibited the SPC-induced contraction. Pertussis toxin (PTX) and specific antibodies to G(i1), G(i2), G(i3) and G(o) inhibited the contraction, implying that SPC-induced contraction depends on PTX-sensitive G(i1), G(i2), G(i3), and G(o) protein. A phospholipase inhibitor U73122 and incubation of permeabilized cells with PLC-beta3 antibody inhibited SPC-induced contraction. The PKC-mediated contraction may be isozyme specific since only PKCepsilon antibody inhibited the contraction. Preincubation with MEK inhibitor PD98059 blocked the SPC-induced contraction, but p38 MAPK inhibitor SB202190 did not. Cotreatment with GF109203X and PD98059 did not show synergistic effects, suggesting that these two kinases are involved in the same signaling pathway in the SPC-induced contraction. The data suggest that S1P-induced contraction in feline esophageal smooth muscle cells depends on activation of the G(i1), G(i2), G(i3) and G(o) proteins and the PLCbeta3 isozyme via the S1P2 receptor, leading to stimulation of a PKCE pathway, which subsequently activates a p44/p42 MAPK pathway.


Asunto(s)
Esófago/efectos de los fármacos , Contracción Muscular/efectos de los fármacos , Miocitos del Músculo Liso/efectos de los fármacos , Fosforilcolina/análogos & derivados , Transducción de Señal/fisiología , Esfingosina/análogos & derivados , Animales , Gatos , Esófago/fisiología , Proteínas de Unión al GTP/fisiología , Proteínas Quinasas Activadas por Mitógenos/fisiología , Miocitos del Músculo Liso/fisiología , Fosfolipasa C beta/fisiología , Fosforilcolina/farmacología , Proteína Quinasa C/fisiología , Receptores de Lisoesfingolípidos/fisiología , Esfingosina/farmacología
18.
Arch Pharm Res ; 30(11): 1419-25, 2007 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-18087810

RESUMEN

We investigated the effects of hydrogen peroxide (H2O2) on relaxation of the cat lower esophageal sphincter (LES). Vasoactive intestinal peptide (VIP) caused dose-dependent relaxation of LES, and H2O2 reduced VIP-induced relaxation. Relaxation was also attenuated by pertussis toxin (PTX), indicating a Gi/o component. VIP treatment increased [35S]GTPgammaS binding to Gs and Gi3 protein, but not to Go, Gq, Gil or Gi2. This increase in Gs or Gi3 binding was reduced by H2O2. However, the relaxation induced by sodium nitroprusside (SNP), 3-morpholino sydnomine (SIN-1), 8-br cGMP (cGMP analog), forskolin (adenylate cyclase activator), and dibutyryl-cAMP (a stable cAMP analog) was not reduced by H2O2. These data suggest that H202 inhibits VIP-induced relaxation via a Gi-dependent pathway, perhaps by inhibiting the activation of G(i3) or Gs downstream of the VIP receptor and independent of cAMP or NO-cGMP signaling.


Asunto(s)
Esfínter Esofágico Inferior/efectos de los fármacos , Peróxido de Hidrógeno/farmacología , Relajación Muscular/efectos de los fármacos , Péptido Intestinal Vasoactivo/farmacología , Animales , Gatos , Colforsina/farmacología , GMP Cíclico/fisiología , Relación Dosis-Respuesta a Droga , Esfínter Esofágico Inferior/fisiología , Femenino , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Masculino
19.
Arch Pharm Res ; 40(2): 204-213, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-28012047

RESUMEN

In a previous study, Quercetin-3-O-ß-D-glucuronopyranoside (QGC) has anti-oxidative and anti-inflammatory effects in vivo. QGC is a flavonoid glucoside extracted from Rumex Aquaticus. We investigated the downstream target proteins involved in IL-1ß-stimulated ROS production and the ability of QGC to inhibit ROS production. Cell viability was determined using the MTT reduction assay. Western blot analysis was performed with antibodies to investigate the activation of three MAPKs, NF-κB, and phosphorylated IκB-α (pIB), and the expression of COX-2. 2',7'-dichlorofluorescin diacetate was used to detect the generation of intracellular ROS species. When the cells were exposed to media containing IL-1ß for 18 h, cell viability was not affected. QGC did not reduce the COX-2 expression induced by IL-1ß. However; QGC attenuated the production of intracellular ROS induced by IL-1ß. IL-1ß increased the expression of ERK, p38 MAPK, and pIB, and nuclear translocation of NF-κB were recovered by the ROS scavenger N-acetyl-L-cysteine (NAC) and QGC, but not by the NADPH oxidase inhibitor diphenylene iodonium. Pretreatment of cells with the ERK inhibitor PD98059, the p38 MAPK inhibitor SB202190, NAC, and QGC attenuated nuclear translocation of NF-κB and activation of pIB. QGC has a scavenging effect on cytokine-induced ROS production, thereby preventing its downstream effects, nuclear translocation of NF-κB, and activation of pIB is mediated by activation of ERK and p38 MAPK, although QGC does not inhibit IL-1ß-stimulated COX-2 expression in feline esophageal epithelial cells. The data suggest that QGC exerts anti-oxidative effects and inhibitory effects against esophageal epithelial cells signals by the action of IL-1ß treatment.


Asunto(s)
Antioxidantes/farmacología , Células Epiteliales/efectos de los fármacos , Interleucina-1beta/metabolismo , Quercetina/análogos & derivados , Especies Reactivas de Oxígeno/metabolismo , Acetilcisteína/farmacología , Animales , Gatos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Ciclooxigenasa 2/metabolismo , Mucosa Esofágica/citología , Flavonoides/farmacología , Fluoresceínas/metabolismo , Quinasa I-kappa B/metabolismo , Imidazoles/farmacología , Proteínas Quinasas Activadas por Mitógenos/metabolismo , FN-kappa B/metabolismo , Fosforilación , Piridinas/farmacología , Quercetina/farmacología , Rumex/química , Transducción de Señal
20.
Mol Cells ; 22(1): 44-50, 2006 Aug 31.
Artículo en Inglés | MEDLINE | ID: mdl-16951549

RESUMEN

We investigated the possible role of p38 MAPK and ETB receptors in ET-1 induction of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) in cultured feline esophageal smooth muscle cells (ESMC). Confluent layers of ESMC were stimulated with 10 nM ET-1 and expression of COX-1 and COX-2, involvement of receptors, and activation of p38 MAPK, were examined by Western blot analysis. Levels of PGE2 induced by ET-1 were measured by Elisa. Using ETA and ETB antagonists (BQ-123 and BQ-788, respectively), the contribution of the ET receptors to COX-1 and COX-2 expression induced by ET-1 was determined. Western blot analysis revealed that treatment of ESMC with ET-1 resulted in transient expression of COX-2 and activation of p38 MAPK. Activation of p38 MAPK was maximal after 1 h. SB202190, a p38 MAPK inhibitor, reduced expression of COX-2, but not COX-1. ET-1-induced release of PGE2 was also blocked by SB202190. COX-2 expression was upregulated only via the ETB receptor, and COX-1 expression was not affected by either antagonist. Taken together, our data suggest that ET-1 causes p38 MAPK-dependent expression of COX-2 by interacting with ETB receptors on ESMC.


Asunto(s)
Ciclooxigenasa 2/biosíntesis , Endotelina-1/fisiología , Esófago/citología , Miocitos del Músculo Liso/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo , Animales , Western Blotting , Gatos , Células Cultivadas , Activación Enzimática , Femenino , Imidazoles/farmacología , Masculino , Oligopéptidos/farmacología , Péptidos Cíclicos/farmacología , Piperidinas/farmacología , Piridinas/farmacología , Receptor de Endotelina A/efectos de los fármacos , Receptor de Endotelina A/fisiología , Receptor de Endotelina B/efectos de los fármacos , Receptor de Endotelina B/fisiología , Proteínas Quinasas p38 Activadas por Mitógenos/antagonistas & inhibidores
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