Defining GME Librarianship: Creating and Developing a New Graduate Medical Education Library Program and Librarian Position.

An academic research institution and a corporate hospital system formed a new graduate medical education (GME) consortium. The consortium objectives were to increase the scholarly activity of the residents and fellows in a national hospital system's GME residency program to match the requirements set forth by the Accreditation Council for Graduate Medical Education. A GME librarian position was created specifically to serve the GME research programs at Florida area hospitals to help with this objective. This paper describes the experience, activities, and lessons learned from the creation of an entirely new GME library program and librarian position for a nine-hospital region in Florida.

Evidence for natural hybridization between native and introduced lineages of Phragmites australis in the Chesapeake Bay watershed.

PREMISE OF THE STUDY: The introduction of nonnative taxa into areas occupied by conspecifics can lead to local extinction of native taxa via habitat modification and competitive dominance, and be exacerbated by outbreeding depression or the formation of invasive hybrid lineages following intraspecific gene flow. The expansion of Eurasian Phragmites australis into tidal wetlands of North America has been accompanied by a dramatic decline of native P. australis, with few relic populations remaining along the Atlantic coastline of the United States, particularly in the Virginia portion of the Chesapeake Bay. METHODS: We sampled populations from the York River and its two major tributaries to determine the pattern of Phragmites invasion and identify remnant native populations that warrant conservation. We used chloroplast DNA haplotypes and nuclear DNA microsatellite profiles to classify individuals as belonging to the native or introduced lineage. KEY RESULTS: Although native Phragmites stands were identified in the brackish upstream reaches of the two York River tributaries, the majority of Phragmites stands surveyed contained the introduced lineage. We also identified a single putative hybrid plant, based on its microsatellite profile. This plant possessed the native cpDNA haplotype and was located in an otherwise native Phragmites stand that is adjacent to an isolated patch of introduced Phragmites. CONCLUSIONS: Although evidence of field hybridization between native and introduced lineages of Phragmites in North America is still relatively rare, the continued encroachment of the introduced lineage into native wetlands may increase the likelihood of future hybrid formation. Careful genetic monitoring to identify remnant native and potential hybrid Phragmites is essential for prioritizing ongoing management efforts.

Methods for characterizing protein acetylation during viral infection.

Lysine acetylation is a prevalent posttranslational modification that acts as a regulator of protein function, subcellular localization, and interactions. A growing body of work has highlighted the importance of temporal alterations in protein acetylation during infection with a range of human viruses. It has become clear that both cellular and viral proteins are decorated by lysine acetylations, and that these modifications contribute to core host defense and virus replication processes. Further defining the extent and dynamics of protein acetylation events during the progression of an infection can provide an important new perspective on the intricate mechanisms underlying the biology and pathogenesis of virus infections. Here, we provide protocols for identifying, quantifying, and probing the regulation of lysine acetylations during viral infection. We describe the use of acetyl-lysine immunoaffinity purification and quantitative mass spectrometry for assessing the cellular acetylome at different stages of an infection. As an alternative to traditional antibody-mediated western blotting, we discuss the benefits of targeted mass spectrometry approaches for detecting and quantifying site-specific acetylations on proteins of interest. Specifically, we provide a protocol using parallel reaction monitoring (PRM). We further discuss experimental considerations that are specific to studying viral infections. Finally, we provide a brief overview of the types of assays that can be employed to characterize the function of an acetylation event in the context of infection. As a method to interrogate the regulation of acetylation, we describe the Fluor de Lys assay for monitoring the enzymatic activities of deacetylases.

Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.

Viral proteins have evolved to target cellular organelles and usurp their functions for virus replication. Despite the knowledge of these critical functions for several organelles, little is known about peroxisomes during infection. Peroxisomes are primarily metabolic organelles with important functions in lipid metabolism. Here, we discovered that the enveloped viruses human cytomegalovirus (HCMV) and herpes simplex virus type 1 (HSV-1) induce the biogenesis of and unique morphological changes to peroxisomes to support their replication. Targeted proteomic quantification revealed a global virus-induced upregulation of peroxisomal proteins. Mathematical modeling and microscopy structural analysis show that infection triggers peroxisome growth and fission, leading to increased peroxisome numbers and irregular disc-like structures. HCMV-induced peroxisome biogenesis increased the phospholipid plasmalogen, thereby enhancing virus production. Peroxisome regulation and dependence were not observed for the non-enveloped adenovirus. Our findings uncover a role of peroxisomes in viral pathogenesis, with likely implications for multiple enveloped viruses.

Trauma-focused CBT for youth with complex trauma.

OBJECTIVES: Many youth develop complex trauma, which includes regulation problems in the domains of affect, attachment, behavior, biology, cognition, and perception. Therapists often request strategies for using evidence-based treatments (EBTs) for this population. This article describes practical strategies for applying Trauma-Focused Cognitive Behavioral Therapy (TF-CBT) for youth with complex trauma. METHODS: TF-CBT treatment phases are described and modifications of timing, proportionality and application are described for youth with complex trauma. Practical applications include (a) dedicating proportionally more of the model to the TF-CBT coping skills phase; (b) implementing the TF-CBT Safety component early and often as needed throughout treatment; (c) titrating gradual exposure more slowly as needed by individual youth; (d) incorporating unifying trauma themes throughout treatment; and (e) when indicated, extending the TF-CBT treatment consolidation and closure phase to include traumatic grief components and to generalize ongoing safety and trust. RESULTS: Recent data from youth with complex trauma support the use of the above TF-CBT strategies to successfully treat these youth. CONCLUSION: The above practical strategies can be incorporated into TF-CBT to effectively treat youth with complex trauma. PRACTICE IMPLICATIONS: Practical strategies include providing a longer coping skills phase which incorporates safety and appropriate gradual exposure; including relevant unifying themes; and allowing for an adequate treatment closure phase to enhance ongoing trust and safety. Through these strategies therapists can successfully apply TF-CBT for youth with complex trauma.