Modulatory properties of curcumin in cancer: A narrative review on the role of interferons.

The immune network is an effective network of cell types and chemical compounds established to maintain the body's homeostasis from foreign threats and to prevent the risk of a wide range of diseases; hence, its proper functioning and balance are essential. A dysfunctional immune system can contribute to various disorders, including cancer. Therefore, there has been considerable interest in molecules that can modulate the immune network. Curcumin, the active ingredient of turmeric, is one of these herbal remedies with many beneficial effects, including modulation of immunity. Curcumin is beneficial in managing various chronic inflammatory conditions, improving brain function, lowering cardiovascular disease risk, prevention and management of dementia, and prevention of aging. Several clinical studies have supported this evidence, suggesting curcumin to have an immunomodulatory and anti-inflammatory function; nevertheless, its mechanism of action is still not clear. In the current review, we aim to explore the modulatory function of curcumin through interferons in cancers.

Immunomodulatory effects of curcumin in systemic autoimmune diseases.

Systemic autoimmune diseases like rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus represent various autoimmune conditions identified by immune system dysregulation. The activation of immune cells, auto-antigen outbreak, inflammation, and multi-organ impairment is observed in these disorders. The immune system is an essential complex network of cells and chemical mediators which defends the organism's integrity against foreign microorganisms, and its precise operation and stability are compulsory to avoid a wide range of medical complications. Curcumin is a phenolic ingredient extracted from turmeric and belongs to the Zingiberaceae, or ginger family. Curcumin has multiple functions, such as inhibiting inflammation, oxidative stress, tumor cell proliferation, cell death, and infection. Nevertheless, the immunomodulatory influence of curcumin on immunological reactions/processes remains mostly unknown. In the present narrative review, we sought to provide current information concerning the preclinical and clinical uses of curcumin in systemic autoimmune diseases.

Novel CoFe2O4@ZnO-CeO2 ternary nanocomposite: Sonochemical green synthesis using Crataegus microphylla extract, characterization and their application in catalytic and antibacterial activities.

In this study, CoFe2O4@ZnO-CeO2 magnetic nanocomposite (CoFe@Zn-Ce MNC) was successfully prepared by facile sonochemical method for the first time. CoFe@Zn-Ce MNC was obtained by green and cost-effective process in the presence of Crataegus microphylla (C. microphylla) fruit extract. Influence of some parameters like capping agents (C. microphylla, SDS and CTAB), sonication time (10, 30 and 60 min) and sonication power (40, 60 and 80 W) were studied to achieve optimum condition. The as-obtained products were characterized by FT-IR, FESEM, TEM, DRS, VSM, EDS, TGA and XRD analysis. Results showed that high magnetic properties (20.38 emug-1), 70-80 nm size and spherical morphology were unique characteristics of synthesized nanocomposite. Antibacterial activity of CoFe@Zn-Ce MNC was examined against E. coli, P. aeruginoss and S. aureus bacteria. Among theme, S. aureus as gram-positive bacteria showed excellent antibacterial activity. Furthermore, photocatalytic performance of the CoFe@Zn-Ce MNC was investigated by degradation of humic acid (HA) molecules under visible and UV light irradiations. The influence of morphology of products and incorporation of cerium oxide with CoFe2O4@ZnO on photocatalytic activity of CoFe2O4@ZnO was performed. After 100 min illumination, the decomposition of HA pollutant by magnetic nanocomposite were 97.2% and 72.4% under exposure of UV and visible light irradiations, respectively. Also, CoFe@Zn-Ce MNC demonstrated high stability in the cycling decomposition experiment after six times cycling runs.

Green and facile synthesis of Ag nanoparticles using Crataegus pentagyna fruit extract (CP-AgNPs) for organic pollution dyes degradation and antibacterial application.

In this work, a cost-effective/green/high-efficient/facile process for the preparation of silver nanoparticles using C. pentagyna (CP-AgNPs) fruit extractas as reducing agent and capping agent was reported. The influence of different parameters including temperature, pH, concentration and time were studied to reach optimum conditions. The as-synthesized CP-AgNPs were characterized using UV/Vis, XRD, SEM, TEM, EDS and DLS analysis. Regular, spherical morphology and homogeneous of CP-AgNPs with a mean particles size of 25-45 nm was determined from TEM images. The CP-AgNPs in presence of sunlight catalyzed the degradation of the organic contaminant dyes, rhodmine b (RhB), eosin (EY) and methylene blue (MB) with percent degradation of 85% and 70% and 78%, respectively. In addition, CP-AgNPs exhibit antibacterial activities against seven ATCC strains of bacteria and eight strains of drug-resistant bacteria. Due to the results, CP-AgNPs enhanced antimicrobial potential against S. aureus, E. faecalis, P. aeruginosa, A. baumannii and E. coli with MIC and MBC of (0.11, 7.1 µg/ml), (0.11, 1.7 µg/ml), (0.11, 0.22 µg/ml), (0.11, 0.22) and (0.11, 0.44 µg/ml), respectively. Generally, CP-AgNPs have excellent potential application for the photocatalytic degradation of organic pollutant and in the development of antibacterial materials.

Effects of bentonite nanoparticles inhalation on lung tissue and blood antioxidant indices in a rat model.

Bentonite is an inorganic clay material that is often easily dispersed as fine particles by air and water circulation, and most people are exposed to different concentrations of bentonite particles. Therefore, the inhaled effects of bentonite nanoparticles (BNPs) were studied in Wistar rats. Seventy-five rats were divided into five groups of 15: four exposure groups (0.1, 0.5, 2, and 10 mg/m3 of BNPs) and one control group. The rats were exposed for 30, 60, and 90 days to BNPs for 5 days a week (6 h/day) in whole-body inhalation chambers. Blood samples were collected to measure the levels of antioxidant activity of the contents such as total antioxidant capacity (TAC) and malondialdehyde (MDA). X-ray diffraction and scanning electron microscopy were used to identify nanoparticles. The results showed no significant difference in the effect of nanoparticles on levels of TAC and MDA in the studied groups based on the concentrations of nanoparticles. However, the level of MDA increased significantly with extending exposure time; there was a significant increase in the level of MDA content 90 days postexposure compared to 30 days postexposure at concentrations of 0.5, 2, and 10 mg/m3. Histopathological examination showed that inhalation exposure of rats to BNPs led to different histopathologic responses in the lung tissue, such as inflammatory infiltration, granulomatous inflammation, acute neutrophilic reaction in the early stages, and lung fibrosis. At the lowest concentration, BNPs have low or no toxicity, and inhalation of these nanoparticles at low concentrations does not affect the levels of MDA and TAC content. However, increased concentration and exposure time caused correspondingly greater increases in MDA and more damage to lung tissue.

Nitrate removal from aqueous solutions by cobalt ferrite nanoparticles synthesized by co-precipitation method: isotherm, kinetic and thermodynamic studies.

The purpose of this study was to examine the nitrate adsorption by cobalt ferrite (CFO) nanoparticles. The adsorbent was synthesized by co-precipitation method and its structure was characterized using scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and vibrating-sample magnetometry. In batch adsorption studies, the effects of various parameters like pH (3-11), adsorbent dose (0.2-0.8 g/L), contact time (5-120 min), initial nitrate concentration (50-200 mg/L), and temperature (283-313 K) on the adsorption process were examined. The results of this study indicated that the maximum adsorption capacity was 107.8 mg/g (optimum condition pH = 3, adsorbent dosage: 0.2 g/L, nitrate concentration: 200 mg/L, contact time: 20 min and temperature: 313 K). The adsorption isotherm had a proper match with Langmuir (R2 = 0.99) and Freundlich (R2 = 0.99) models. The adsorption of nitrate by CFO followed pseudo-second-order kinetics. The results of the thermodynamics of the nitrate adsorption process by CFO showed that all the values of Gibbs free energy change, enthalpy change and entropy change were positive. Therefore, this process was endothermic and non-spontaneous.

Assessment of carbon monoxide concentration in indoor/outdoor air of Sarayan city, Khorasan Province of Iran.

Nowadays, air pollution by humans is considered a serious problem. One of the main sources of air pollution is carbon monoxide which is called the silent killer. With the increasing rate of population growth in Iran and subsequent acceleration of vehicle and fossil fuel usage, the mortality rate of carbon monoxide has increased. The aim of this study is assessment of the concentration of carbon monoxide in indoor and outdoor air of Sarayan city, Khorasan Province of Iran. In this descriptive-analytic study, the air condition of a residential area and outdoor environment of Sarayan city has been monitored for determining the carbon monoxide concentration during a 3-month period from January to March 2017. Overall, 25 stations with uniform distribution were located in the city. Fifty samples were taken monthly and by considering 3-month period, a total of 150 samples were gathered. The samples were taken in kitchens between 11 a.m. and 3 p.m., and by considering the respiratory height of the human body, the carbon monoxide meters (TES model, Taiwan) were placed at a height of 75-150 cm. The descriptive statistics were presented after entering data into SPSS-16, and, by applying Mann-Whitney and Kruskal-Wallis tests in the case of α = 0.05, the data were analyzed. The average concentrations of carbon monoxide in indoor and outdoor environments were 0.84 ± 3.21 ppm and 0.27 ± 0.92 ppm, respectively. The maximum carbon monoxide concentration in February in an outdoor environment was 6 ppm (station no. 4) and the least one was 0 ppm. In March, for an indoor area, the maximum carbon monoxide concentration was 41 pm for station no. 11. The indoor-to-outdoor (I-to-O) ratio in March was higher than other months, and in January, was less than others. The study reveals that the indoor and outdoor carbon monoxide indices of Sarayan city are at acceptable levels.

Bentonite and montmorillonite nanoparticles effectiveness in removal of fluoride from water solutions.

The aim of this study was to evaluate the effectiveness of montmorillonite and bentonite nanoparticles in removal of fluoride from water solutions. This experimental study has been conducted in batch condition in which the effects of different parameters such as contact time, pH, initial concentration of fluoride and amount of adsorbent mass have been investigated. Finally thermodynamics, isotherm and kinetics of the both adsorbents have been studied. The maximum adsorption capacity for both adsorbents occurred at fluoride concentration of 20 mg/L, contact times of 60 minutes, pH = 3 and adsorbent mass of 0.25 g/L. The adsorption process was exothermic and the result of the Langmuir and Freundlich isotherm study show that bentonite nanoparticles behave more similar to the Langmuir isotherm model and montmorillonite nanoparticles behave according to the both isotherms. Furthermore, the adsorption of fluoride by bentonite in all studied fluoride concentrations and montmorillonite in higher fluoride concentrations followed pseudo second-order kinetics.

Synthesis of walnut shell modified with titanium dioxide and zinc oxide nanoparticles for efficient removal of humic acid from aqueous solutions.

The main aim of this research was to study the efficiency of modified walnut shell with titanium dioxide (TiO2) and zinc oxide (ZnO) in the adsorption of humic acid from aqueous solutions. This experimental study was carried out in a batch condition to determine the effects of factors such as contact time, pH, humic acid concentration, dose of adsorbents (raw walnut shell, modified walnut shell with TiO2 and ZnO) on the removal efficiency of humic acid. pHzpc of raw walnut shell, walnut shell modified with TiO2 and walnut shell modified with ZnO were 7.6, 7.5, and 8, respectively. The maximum adsorption capacity of humic acid at concentration of 30 mg/L, contact time of 30 min at pH = 3 in an adsorbent dose of 0.02 g of walnut shell and ZnO and TiO2 modified walnut shell were found to be 35.2, 37.9, and 40.2 mg/g, respectively. The results showed that the studied adsorbents tended to fit with the Langmuir model. Walnut shell, due to its availability, cost-effectiveness, and also its high adsorption efficiency, can be proposed as a promising natural adsorbent in the removal of humic acid from aqueous solutions.

Crimean-Congo Hemorrhagic Fever Virus Clade IV (Asia 1) in Ticks of Western Iran.

Crimean-Congo Hemorrhagic Fever virus (CCHFV) is transmitted through the bite of an infected tick, or by direct contact with CCHFV-infected patients' blood or the products of infected livestock. In 2012, ticks were collected in eight regions of Lorestan Province, Iran. In total, 434 ticks were collected. Reverse transcriptase polymerase chain reaction was used for the detection of CCHFV RNA. Of 434 ticks, 419 (96.6%) ticks were from the family Ixodidae (hard ticks) and 15 (3.5%) ticks were from the family Argasidae (soft ticks). The presence of CCHFV RNA was detected in 29 (6.7%) of 434 ticks. The infected tick species include Hyalomma asiaticum (n = 7, 7.4%), Hyalomma anatolicum (n = 12, 13.2%), Hyalomma marginatum (n = 1, 16.7%), and Rhipicephalus sanguineus (n = 9, 4.3%). These empirical data demonstrated that the majority of CCHFV-positive ticks belonged to the Ixodidae. None of the Argasidae and Haemaphysalis sulcata species was infected with CCHFV. The phylogenetic analyses of the tick-derived CCHFV strains revealed that all 29 viral strains fell in clade IV (Asia 1). The most abundant species of tick collected in this study was R. sanguineus followed by different species of Hyalomma. Given the infection rate among collected ticks, H. marginatum was the most abundant infected tick species (16.7%) followed by H. anatolicum (13.2%), H. asiaticum (7.4%), and R. sanguineus (4.3%).

The Effect of Statins on the Differentiation and Function of Central Nervous System Cells.

Statins (3-hydroxy-3-methylglutaryl-CoA reductase inhibitors) reduce plasma cholesterol and improve endothelium-dependent vasodilation, inflammation, and oxidative stress. The effect of statins on the central nervous system (CNS), particularly on cognition and neurological disorders such as cerebral ischemic stroke, multiple sclerosis (MS), and Alzheimer's disease (AD), has received increasing attention in recent years, both within the scientific community and in the media. This review aims to provide an updated discussion on the effects of statins on the differentiation and function of various nervous system cells, including neurons and glial cells. Additionally, the mechanisms of action and how different types of statins enter the CNS will be discussed.

A systematic review of photocatalytic degradation of humic acid in aqueous solution using nanoparticles.

OBJECTIVES: Humic acid (HA) compounds in the disinfection processes of drinking water and wastewater are considered as precursors of highly toxic, carcinogenic and mutagenic disinfectant by-products. The aim of this study was to systematically review all research studies on the photocatalytic degradation of humic acid and to evaluate the laboratory conditions and results of these studies. CONTENT: The present systematic review was performed by searching the Scopus, PubMed, and web of science databases until December 2021. The parameters of type of catalyst, catalyst size, optimum pH, optimum initial concentration of humic Acid, optimum catalyst concentration, optimum time, light used and removal efficiency were investigated. SUMMARY: 395 studies were screened and using the inclusion and exclusion criteria, in total, 20 studies met our inclusion criteria and provided the information necessary to Photocatalytic degradation of humic acid by nanoparticles. In the investigated studies, the percentage of photocatalytic degradation of humic acid by nanoparticles was reported to be above 70%, and in some studies, the removal efficiency had reached 100%. OUTLOOK: From the results of this systematic review, it was concluded that the photocatalytic process using nanoparticles has a high effect on the degradation of humic acid.

Biosynthesis of MnFe2O4@TiO2 magnetic nanocomposite using oleaster tree bark for efficient photocatalytic degradation of humic acid in aqueous solutions.

The presence of humic acid compounds in water resources, as one of the precursors of Trihalomethanes and Holoacetic acids, causes health problems for many communities. The aim of this research study was to investigate the photocatalytic degradation efficiency of humic acid using MnFe2O4@TiO2 nanoparticles which produced by green synthesis method. The synthesis of metal nanoparticles using plant extracts and the study of their catalytic performance is a relatively new topic. Many chemical techniques have been proposed for the synthesis of MnFe2O4@TiO2 nanoparticles, but green synthesis has received much attention due to its availability, simplicity, and non-toxicity. The properties of synthesized nanoparticles were determined by SEM, FT-IR, XRD, EDS, and DLS analysis. The results of the study showed that under optimal experimental conditions (pH = 3, nanocomposite dose = 0.03 g/L, humic acid initial concentration = 2 mg/L, and contact time = 20 min), it is possible to achieve maximum degradation of humic acid. Therefore; MnFe2O4@TiO2 nanoparticles have high efficiency for removing of humic acid from aqueous solutions under UV light.

Characterization of the angiotensin-converting enzyme 2 (ACE2), the main receptor for the SARS-CoV-2 virus.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes Coronavirus Disease 2019 (COVID-19), one of the deadliest medical difficulties to affect people in more than a century. The virus has now spread to many countries worldwide, posing a big challenge to the health status of people in affected populations. Gaining more knowledge about the different aspects of this virus will lead us to better control and treatment methods. In this paper, we discuss the SARS-CoV-2 structure and the mechanism of this virus's entry into host cells through angiotensin-converting enzyme 2 (ACE2), the main receptor for the SARS-CoV-2 virus. The main connection between SARS-CoV-2 and ACE2 is Spike protein. Other topics are also included, like ACE2 structure, functions, and physiology. For instance, ACE2 is involved in the renin-angiotensin-aldosterone system, Angiotensin A/ACE2/Alamandine/MAS-Related GPCR D (MrgD) Axis, the Kinin-Kallikrein System. It also acts as Chaperone Protein for the Amino Acid Transporter, B0AT1, and has a connection with Apelin Peptides. Since ACE2 plays a primary role in COVID-19 pathogenesis, scientists have discovered some SARS-CoV-2 therapy methods based on ACE2 targeting. Tissue expression in different genders and ages, polymorphisms, and host epigenetics, the role of ACE2 in hypertension, and cytokine storm are explained separately.

The Role of Inflammatory Cytokines (Interleukin-1 and Interleukin-6) as a Potential Biomarker in the Different Stages of COVID-19 (Mild, Severe, and Critical).

Cytokine storm refers to the overproduction of immune and inflammatory cells and their proteins (cytokines) [interleukin (IL)-1 and IL-6] causing acute respiratory distress syndrome in COVID-19. COVID-19 causes inflammatory reactions, and patients with COVID-19 had categorized as mild, severe, and critical after reviewing previous studies. Then, it is crucial to find immune-inflammatory indicators that might predict the disorder severity and the prognosis primarily for guiding medical therapy in the face of this unexpectedly developing unique infectious disease. Higher levels of IL-6 and IL-1 levels might be seen in patients with COVID-19 at each stage. In addition, IL-1-induced IL-6 assists in the synthesis of liver C-reactive protein (CRP) in acute phase responses. Recent studies suggested that IL-6 levels are an independent predictor of COVID-19 illness because they were significantly higher in patients with severe than with mild COVID-19 symptoms. Anakinra and tocilizumab (TCZ) are beneficial in lowing mortality in COVID-19 patients; however, information on their safety and efficacy is scarce. The aim of this study was to investigate the role of inflammatory cytokines (IL-1 and IL-6) as potential biomarkers in the different stages (mild, severe, and critical) of COVID-19. A systematic search during the years 2021-2022 using the keywords SARS-CoV-2, COVID-19, IL-6, IL-1, CRP, mild stage, severe stage, critical stage, cytokine storm, tocilizumab, and anakinra was performed in PubMed and Google Scholar databases. This study reviews studies that have investigated the role of high levels of these cytokines in the severity of the disease in patients with COVID-19 and the inhibitory function of TCZ and anakinra in preventing mechanical ventilation and patient mortality. According to the result, studies suggest that decreased innate immune response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in association with the production of inflammatory cytokines is the determining and driving function of COVID-19.

Heavy metals in contact dermatitis: A review.

Contact dermatitis is an inflammatory skin reaction caused by direct contact with chemical substances in the environment and can either be irritant or allergic in nature. The clinical symptoms of contact dermatitis, include local skin rash, itching, redness, swelling, and lesions. Nowadays, 15-20% of people have some degree of contact dermatitis, which can be more or less severe. Immune responses in allergic contact dermatitis (ACD) are due to the effects of cytokines and allergen-specific CD4+ and CD8+ T cells on the skin. Acids and alkalis such as drain cleaners, plants such as poinsettias, hair colors, and nail polish remover, are all prominent causes of irritant contact dermatitis (ICDs). Heavy metals are metallic elements with a high atomic weight that are hazardous in low quantities and are known to cause dermatitis after systemic or local exposure. Nickel (Ni), chromium (Cr), lead (Pb), and copper (Cu) are among the most common heavy metals used in various industries. Metal allergies may cause ACD and also systemic contact dermatitis (SCD). Contact dermatitis is detected by laboratory tests such as patch testing, lymphocyte stimulation test (LST), and evaluation of cytokine production by primary cultures of peripheral blood mononuclear cells. This article presents an update on the epidemiological and clinical characteristics of ACD and SCD caused by three heavy metals (Cr, Cu, and Pb). Ni is not discussed due to recent coverage. Furthermore, the effects of contact sensitivity to some other heavy metals, such as gold (Au), cobalt (Co), palladium (Pd), and mercury (Hg) are discussed.

Systemic allergic contact dermatitis to palladium, platinum, and titanium: mechanisms, clinical manifestations, prevalence, and therapeutic approaches.

Contact dermatitis (CD) is an inflammatory skin disease of eczema that is elicited by chemicals or metal ions that have toxic effects without eliciting a T-cell response (contact elicitation) or by small reactive chemicals that modify proteins and induce innate and adaptive immune responses (contact allergens). The clinical condition is characterized by localized skin rash, pruritus, redness, swelling, and lesions, which are mainly detected by patch tests and lymphocyte stimulation. Heavy metals such as palladium (Pd), platinum (Pt), and titanium (Ti) are ubiquitous in our environment. These heavy metals have shown CD effects as allergic agents. Immunological responses result from the interaction of cytokines and T cells. Occupational metal CD accounts for most cases of work-related cutaneous disorders. In this systematic review, the allergic effects of heavy metals, including Pd, Pt, and Ti, and the mechanisms, clinical manifestations, prevalence, and therapeutic approaches are discussed in detail. Furthermore, the therapeutic approaches introduced to treat CD, including corticosteroids, topical calcineurin inhibitors, systemic immunosuppressive agents, phototherapy, and antihistamines, can be effective in the treatment of these diseases in the future. Ultimately, the insights identified could lead to improved therapeutic and diagnostic pathways.

Wound Healing Effects of Chitosan Nanosheets/Honey Compounds in Male BALB/c Mice.

Background and aim: Up to now, proper wound care management has remained as an important clinical challenge. Chitosan nanosheets (CNSs) showed a great potential in tissue engineering, but our knowledge about their wound healing effectiveness is based on very limited data. Thus, the aim of this research was to evaluate the wound healing potential of CNSs and honey as a vehicle for these nanoparticles. Methods: The skin excisional wound injury model was made in adult male BALB/c mice (n = 60) by creating two identical sized wounds (5mm) on either side of their dorsal midline. The animals were divided into five groups (n = 12 each) as untreated control, honey, polyethylene glycol, and CNSs dissolved either in honey or polyethylene glycol. Animals were received their relative topical treatments twice per day for 14 consecutive days. Tissue sampling was carried out on days 4, 7, 10, and 14 post wounding. The histological parameters including inflammatory cells infiltration, fibroblast proliferation, re-epithelialization, granulation formation, and collagen formation were evaluated in all studied time points. Results: Compared to the control group, CNSs showed significant wound healing activities with lower inflammatory cells infiltration, higher fibroblastosis and new epithelium thickness, and greater granulation area and collagen fibers density in the ulcer bed. In addition, honey synergistically increased the wound healing activity of the studied nanoparticles. Conclusion: These results showed that CNSs have promising wound healing activity specially when dissolved with honey concurrently.

Environmental arsenic exposure and its toxicological effect on thyroid function: a systematic review.

OBJECTIVES: This study was performed to review epidemiological evidence related to Arsenic (As) effects on the thyroid function by focusing on the serum thyroid hormone concentration. CONTENT: As, one of the main pollutants, has been recognized as an endocrine-disrupting agent that may affect the function of thyroid as shown by experimental studies. SUMMARY: This systematic study indicates the association between As exposure and thyroid dysfunction. The studies have shown an association between serum and urine concentration of arsenic and thyroid dysfunction. Most of them reported the association between increase in the serum or urine As levels and decrease in the triiodothyronine (T3) and thyroxine (T4), and also elevation in the thyrotropic hormone (TSH) levels. OUTLOOK: Our findings related to the effects of As on the function of thyroid in humans are still limited and future studies should be done to address this question.

Bentonite Nanoparticles and Honey Co-Administration Effects on Skin Wound Healing: Experimental Study in the BALB/c MICE.

In recent years, nanotechnology and the subsequent production of nanoparticles have developed excellent methods for medical applications, including wound healing. One of these nanoparticles is bentonite nanoparticles (BNPs) which show high ability in tissue engineering. But our knowledge of its effectiveness in wound healing is based on little data. Therefore, the main purpose of this study was to evaluate the wound healing ability of BNPs and in the next step the suitability of honey as a solvent for these nanoparticles. Methods: In this experimental study, an excisional wound injury model was developed in adult male BALB/c mice (n = 60) by creative two equal-sized wounds (5 mm) on either side of their back midline. The animals were allocated into five groups (n = 12 each) as untreated control (U), honey (H), polyethylene glycol (P), and (BNPs) dissolved in honey or polyethylene glycol (H + BNPs, P + BNPs). Animals have received their relative topical treatments twice per day for 14 consecutive days. Tissue sampling was carried out on days 4, 7, 10, and 14. The tissue sections were stained with hematoxylin and eosin and Trichrome-Masson staining methods. The histomorphological parameters including inflammatory cells infiltration, fibroblasts, re-epithelialization, granulation formation, and collagenases were evaluated in all tissue sections. Data were analyzed by SPSS 16 software. Comparison between the groups was performed by one-way analysis of variance following Tukey's post-hoc test. Compared to the control group, BNPs showed significant wound healing activities with lower inflammatory cells infiltration, higher fibroblastosis and new epithelium thickness, and greater granulation area and collagen fibers density in the ulcer bed. In addition, honey as a solvent synergistically increased the wound healing activity of the studied nanoparticle. These results for the first time are clearly showing that BNPs have a promising wound healing activity, especially when applied with honey concurrently.