RESUMEN
OBJECTIVE: To characterize peripheral blood monocytes in uveitis associated with juvenile idiopathic arthritis (JIAU). METHODS: Peripheral blood monocytes from children with JIA (either with (nâ¯=â¯18) or without uveitis (nâ¯=â¯11)), idiopathic anterior uveitis (IAU; nâ¯=â¯12) and healthy controls (nâ¯=â¯11) were analyzed by flow cytometry. RESULTS: Percentage of CD14â¯+â¯CD86+ monocytes and CD86 expression on single cell level were significantly higher in all patient groups than in controls, whereas no major differences existed between patient groups. Frequency of CD39+ (pâ¯<â¯0.05 all groups) and CD73+ monocytes (pâ¯=â¯0.03 JIAU vs controls) was elevated in patients. Disease activity did not influence monocyte phenotypes, but in methotrexate-treated JIAU patients numbers of CCR2+ monocytes were reduced and numbers of CD86+ and CD39+ cells increased. CONCLUSION: Children with arthritis or uveitis display a distinct monocytic phenotype when compared to cells from healthy children. Phenotypic changes seem to be neither arthritis- nor uveitis-dependent, but may be modified by treatment.
Asunto(s)
Artritis Juvenil/inmunología , Monocitos/inmunología , Uveítis Anterior/inmunología , Uveítis/inmunología , Adolescente , Antirreumáticos/uso terapéutico , Artritis Juvenil/sangre , Artritis Juvenil/tratamiento farmacológico , Antígeno B7-2/inmunología , Antígeno B7-2/metabolismo , Niño , Preescolar , Femenino , Humanos , Lactante , Receptores de Lipopolisacáridos/inmunología , Receptores de Lipopolisacáridos/metabolismo , Masculino , Metotrexato/uso terapéutico , Monocitos/efectos de los fármacos , Monocitos/metabolismo , Resultado del Tratamiento , Uveítis/sangre , Uveítis/tratamiento farmacológico , Uveítis Anterior/sangre , Uveítis Anterior/tratamiento farmacológicoRESUMEN
Purpose: To characterize peripheral blood T cells in juvenile idiopathic arthritis-associated uveitis (JIAU). Methods: Blood samples were taken from children with JIAU (n = 18), JIA without ocular involvement (n = 11), idiopathic anterior uveitis (IAU, n = 12), and healthy controls (n = 11). Cells were stained for T cell surface markers, and intracellular cytokine staining was performed after cell stimulation and analyzed by flow cytometry. Results: The Th1/Th2 ratio was increased in JIAU patients. Numbers of IL-13-expressing cells an level of IL-13 and IL-10 expression per cell were increased in all patient groups; whereas, percentages of IL-5-expressing T cells were decreased. Numbers of proinflammatory Th17 cells and T cells expressing CTLA-4 were increased in all patient groups; whereas, γ/δ T cell numbers were decreased. Results from JIA and IAU were similar. Conclusion: T cell subtypes and potential T cell function are altered in pediatric patients with uveitis and arthritis as compared to healthy children.