Optical fingerprinting of peptides using two-dimensional infrared spectroscopy: proof of principle.

We employ a particular form of two-dimensional infrared four-wave mixing (2DIR FWM) as a vibrational spectroscopic tool to quantify the amino acid content of a number of peptides. Vibrational features corresponding to ring modes of the aromatic groups of phenylalanine (Phe) and tyrosine (Tyr), as well as a methylene mode that is used as an internal reference, are identified. We show that the ratios of the integrated intensities, and the amplitudes, of the aromatic peaks of Phe and Tyr relative to the methylene integrated intensity, and amplitude, are proportional to the actual ratio of Phe and Tyr to CH(2) in the samples within a precision of +/-12.5%. This precision is shown to be sufficient to use this form of 2DIR spectroscopy as a possible proteins fingerprinting tool.

Heterogeneity of water at the phospholipid membrane interface.

Femtosecond infrared (IR) two-color pump-probe experiments were used to investigate the nonlinear response of the D2O stretching vibration in weakly hydrated dimyristoyl-phosphatidylcholine (DMPC) membrane fragments. The vibrational lifetime is comparable to or longer than that in bulk D2O and is frequency dependent, as it decreases with increasing probe frequency. Also, the lifetime increases when the water content of the sample is lowered. The measured lifetimes range between 903 and 390 fs. A long-lived spectral feature grows in following the excitation and is attributed to photoinduced D-bond breaking. The photoproduct spectrum differs from the steady state difference Fourier transform infrared (FTIR) spectrum, showing that the full thermalization of the excitation energy happens on a much longer time scale than the time interval considered (12 ps). Further evidence of the inhomogeneous character of the water residing in the polar region of the bilayer comes from the spectral anisotropy. The water molecules absorbing on the low frequency side of the absorption band show no decay at all of the anisotropy, while an ultrafast partial decay appears when the high frequency side of the spectrum is probed. The overall behavior differs remarkably from that observed with similar experiments in bulk water and in water segregated in inverse micelles. In weakly hydrated phospholipid membranes, water molecules are present mostly as isolated species, prevalently involved in strong, rigid, and persistent hydrogen bonds with the polar groups of the bilayer molecules. This specific character appears to have a direct effect on the structural stability and thermal properties of the membrane.

Distinct water species confined at the interface of a phospholipid membrane.

The physics of confined water has stimulated extensive research in recent years, in particular, regarding the role of hydrogen bonding as a significant factor in the observed dynamics. In this work, two-dimensional infrared spectroscopy was employed to investigate the response of the OH moiety of water in phospholipid membrane samples. The results show strong evidence for three distinct hydrogen bonding motifs (H2O with zero, one, or both OH moieties hydrogen bonded), whose relative proportions at the membrane interface are estimated.

Time-resolved studies of water dynamics and proton transfer at the alumina-air interface.

The present study aims to understand the dynamical properties of water and OH groups layered on an alumina surface mainly by means of femtosecond IR-pump IR-probe transient absorption spectroscopy. The experimental results obtained demonstrate the existence of several kinds of O-H vibrators on the surface of alumina membranes, distinguishing them by their behavior on the femtosecond time scale and by the anisotropy of their spectral response. In the high-frequency region (>3400 cm-1), the absorption is due to well-packed aluminol groups and to physisorbed water patches on the surface. When pumping at 3200 cm-1, physisorbed water hydrogen-bonded to AlOH2+ groups is observed. The anisotropy measurements demonstrate the existence of an efficient energy-transfer mechanism among the water molecules characterized by a time constant of 400 +/- 100 fs. The persisting anisotropy at long times, especially in the case of AlOH groups and of the structured physisorbed water layer on top of them, proves the anisotropic structuring induced by the surface. The excitation at 3000 cm-1 enables the detection of a photon-induced proton-transfer reaction. The proton back-transfer reaction time constant is 350 +/- 50 fs. From anisotropy measurements, we estimate the proton hopping time to be 900 +/- 100 fs in a locally extended water network lying on the surface.

Direct identification and decongestion of Fermi resonances by control of pulse time ordering in two-dimensional IR spectroscopy.

We show that it is possible to both directly measure and directly calculate Fermi resonance couplings in benzene. The measurement method used was a particular form of two-dimensional infrared spectroscopy (2D-IR) known as doubly vibrationally enhanced four wave mixing. By using different pulse orderings, vibrational cross peaks could be measured either purely at the frequencies of the base vibrational states or split by the coupling energy. This capability is a feature currently unique to this particular form of 2D-IR and can be helpful in the decongestion of complex spectra. Five cross peaks of the ring breathing mode nu13 with a range of combination bands were observed spanning a region of 1500-4550 cm(-1). The coupling energy was measured for two dominant states of the nu13+nu16 Fermi resonance tetrad. Dephasing rates were measured in the time domain for nu13 and the two (nu13+nu16) Fermi resonance states. The electronic and mechanical vibrational anharmonic coefficients were calculated to second and third orders, respectively, giving information on relative intensities of the cross peaks and enabling the Fermi resonance states of the combination band nu13+nu16 at 3050-3100 cm(-1) to be calculated. The excellent agreement between calculated and measured spectral intensities and line shapes suggests that assignment of spectral features from ab initio calculations is both viable and practicable for this form of spectroscopy.