RESUMEN
A novel turn-on fluorescent 8-amino BODIPY-based probe carrying a thiourea unit as the mercury ion recognition unit has been developed. Due to the cascade reaction processes, consecutive color changes reflecting the electronic absorption and emission responses were observed upon addition of increased concentrations of mercury(ii) ions. The likely sensing mechanism was proposed as mercury ion-promoted cyclization and subsequent hydrolysis. The probe displayed a selective response to mercury ions over other metal ions. Additionally, experiments with living Human Hepatoma SMMC-7721 cells to visualize intracellular mercury ions in biological systems were carried out with the probe.
Asunto(s)
Compuestos de Boro , Colorantes Fluorescentes , Mercurio/análisis , Línea Celular Tumoral , Humanos , Iones , Espectrometría de FluorescenciaRESUMEN
A highly selective and sensitive turn-on red fluorescent 1-amino BODIPY-based probe (where BODIPY denotes indole-based boron-dipyrromethene) with high off-to-on contrast ratio has been developed. The probe displayed selective response to thiophenols over aliphatic thiols. Probe 1 is promising for the quantitative detection of thiophenol with a linear response from 6 × 10(-6) M to 1 × 10(-4) M, and the detection limit for thiophenol (PhSH) reaches 4 × 10(-6) M measured in acetonitrile/PBS buffer. The detection limit could be improved to 37 nM (detection limit to 4 ppb) in water when 1% Tween 20 was used to assist the dissolvation of probe 1 in water. Probe 1 is also a useful fluorescent probe for detecting thiophenols in living cells in red emission, which may greatly improve the detectable sensitivity.
Asunto(s)
Compuestos de Boro , Medios de Contraste , Colorantes Fluorescentes , Fenoles/análisis , Compuestos de Sulfhidrilo/análisis , Compuestos de Boro/química , Carcinoma Hepatocelular , Medios de Contraste/química , Cristalografía por Rayos X , Colorantes Fluorescentes/química , Humanos , Concentración de Iones de Hidrógeno , Procesamiento de Imagen Asistido por Computador , Límite de Detección , Neoplasias Hepáticas , Microscopía Fluorescente , Sensibilidad y Especificidad , Células Tumorales CultivadasRESUMEN
A highly selective and sensitive turn-on fluorescent BODIPY-based probe for the simultaneous and selective detection of Cys and Hcy/GSH from dual emission channels was developed. The spatial steric hindrance of the methyl groups at 1- and 7-positions in BODIPY skeleton prevented intramolecular displacement of sulfur with amino group of Hcy but not of Cys. GSH molecular skeleton is larger and amino is far away from sulfydryl group, and the product of the reaction of probe with GSH canstay in thiol phase. Therefore, the probe was successfully applied to the detection of Cys from GSH/Hcy. The confocal microscopy experiments implied that this probe is a promising candidate for imaging of Cys and Hcy/GSH in Hela cells.
Asunto(s)
Cisteína/análisis , Colorantes Fluorescentes/química , Glutatión/análisis , Homocisteína/análisis , Compuestos de Boro , Células HeLa , Humanos , Modelos Lineales , Microscopía Confocal , Imagen Molecular , Sensibilidad y EspecificidadRESUMEN
A new selective fluorescent and colorimetric chemosensor for the detection of GSH was developed. The discrimination of GSH from Cys and Hcy is achieved through two emission channel detection. The detection limit of probe 1 for GSH reached 10 nM (3 ppb). The excellent sensitivity and selectivity of probe 1 allow the selective detection of GSH over Cys and Hcy, which can be visualized colorimetrically and/or fluorescently. The sensitive detection of GSH allowed for convenient measurement of the GSH content in human plasma. The presence of GSH in cells was demonstrated through cell imaging.