RESUMEN
The mechanical properties of bioceramic conformed pieces based on micelle-templated silica (MTS) such as SBA15, MCM41 and MCM48 as well as MTS/agarose systems have been evaluated under static and cyclic compressive tests. The MTS pieces exhibited a brittle behaviour. Agarose, a biocompatible and biodegradable hydrogel, has been used to shape ceramic-agarose pieces following a low temperature shaping method. Agarose conferred toughness, ductility and a rubbery consistency up to a 60% strain in ceramic MTS/agarose systems leading to a maximum strength of 10-50 MPa, without losing their initial cylindrical structure. This combination of ceramic and organic matrix contributes to avoiding the inherent brittleness of the bioceramic and enhances the compression resistance of hydrogel. The presence of mechanical hysteresis, permanent deformation after the first cycle and recovery of the master monotonous curve of MTS/agarose systems indicate a Mullins-like effect similar to that found in carbon-filled rubber systems. We report this type of mechanical behaviour, the Mullins effect, for the first time in MTS bioceramics and MTS bioceramic/agarose systems.
Asunto(s)
Micelas , Sefarosa/química , Dióxido de Silicio/química , Materiales Biocompatibles/química , Carbono/química , Cerámica/química , Fuerza Compresiva , Hidrogeles/química , Ensayo de Materiales , Fenómenos Mecánicos , Microscopía Electrónica de Rastreo/métodos , Compuestos Orgánicos/química , Presión , Goma/química , Estrés Mecánico , TemperaturaRESUMEN
Orthopaedic materials may improve its capacity to resist bacterial adherence, and subsequent infection. Our aim was to test the bacterial adherence to alpha-tocopherol (frequently named vitamin E, VE) doped or blended UHMWPE with S. aureus and S. epidermidis, compared to virgin material. Collection strains and clinical strains isolated from patients with orthopaedic infections were used, with the biofilm-developing ability as a covariable. While collection strains showed significantly less adherence to VE-UHMWPE, some clinical strains failed to confirm this effect, leading to the conclusion that VE doped or blended UHMWPE affects the adherence of some S. epidermidis and S. aureus strains, independently of the concentration in use, but the results showed important intraspecies differences and cannot be generalized.
Asunto(s)
Adhesión Bacteriana/efectos de los fármacos , Polietilenos/química , Staphylococcus aureus/efectos de los fármacos , Staphylococcus epidermidis/efectos de los fármacos , Vitamina E/química , Vitamina E/farmacología , Materiales Biocompatibles/química , Interacciones Hidrofóbicas e Hidrofílicas , Espectroscopía de Fotoelectrones , Staphylococcus aureus/fisiología , Staphylococcus epidermidis/fisiología , Propiedades de SuperficieRESUMEN
Ultra-high molecular weight polyethylene (UHMWPE) is a relevant thermoplastic in industry and a well-proven standard biomaterial in joint replacements. To enhance its tribological properties while preserving its bulk ones, composite coatings on a UHMWPE substrate were prepared using non-functionalised graphene nanoplatelet (GNP) at reinforcement concentration of 0.1-5 wt% and two mechanical mixing techniques (ball mill or blade mixer) with different consolidation temperatures of 175-240 °C. Changes in morphology and size of the UHMWPE particles before hot-pressing were observed in function of the mechanical mixing techniques applied. Wear rate was affected by graphene content, reaching a minimum at 0.5 wt% GNP, with a reduction of 20 and 15%, for ball milling and blade mixer, respectively. However, blade mixer increased the wear rate by around twice respect the ball milling results, for all the studied materials. The coefficient of friction decreased notably, by ~25%, below 3 wt% GNP content, and hardness increased by 24%, regardless of the mechanical mixing process used. Finally, consolidation temperature had a positive influence on wear rate at temperatures of around 195 °C, which could be related to the free radical scavenger effect of the GNP.
Asunto(s)
Grafito , Materiales Biocompatibles , Ensayo de Materiales , PolietilenosRESUMEN
Single walled carbon nanotubes (SWNT) have been incorporated into a polypropylene (PP) matrix in different concentrations (range: 0.25-2.5 wt%). The nanotubes were blended with PP particles (approximately 500 microm in size) before mixing in an extruder. Finally, rectangular plates were obtained by compression moulding. PP-SWNT composites were gamma irradiated at different doses, 10 and 20 kGy, to promote crosslinking in the matrix and potentially enhance the interaction between nanotubes and PP. Extensive thermal, structural and mechanical characterization was conducted by means of DSC, X-ray diffraction, Raman spectroscopy, uniaxial tensile tests and dynamic mechanical thermal (DMTA) techniques. DSC thermograms reflected higher crystallinity with increasing nanotube concentration. XRD analysis confirmed the only presence of a monoclinic crystals and proved unambiguously that CNTs generated a preferred orientation. Raman spectroscopy confirmed that the intercalation of the polymer between bundles is favored at low CNTs contents. Elastic modulus results confirmed the reinforcement of the polypropylene matrix with increasing SWNT concentration, although stiffness saturation was observed at the highest concentration. Loss tangent DMTA curves showed three transitions for raw polypropylene. While gamma relaxation remained practically unchanged in all the samples, beta relaxation temperatures showed an increase with increasing CNT content due to the reduced mobility of the system. Gamma-irradiated PP exhibited an increase in the beta relaxation temperature, associated with changes in glass transition due to radiation-induced crosslinking. On the contrary, gamma-irradiated nanocomposites did not show this effect probably due to the reaction of radiative free radicals with CNTs.
RESUMEN
Ion implantation may provide medical polyethylenes with excellent mechanical and tribological properties, helping to lower the risk of long-term osteolysis. Highly crosslinked and remelted polyethylenes, materials currently used as soft components in artificial joints, were implanted with N+ and He+ ions at different ion fluences. The mechanical and tribological properties under distilled water lubrication at body temperature were assessed after ion implantation by means of microhardness and pin-on-disc tests respectively. Thus, the influences of the ionic species and implantation dose on surface hardness, friction coefficient, and wear factor were fully characterized. Furthermore, the tribological behaviour was evaluated after an accelerated ageing protocol (120 degrees C for 36h). Ion implantation increased the surface hardness, as well as friction coefficients, and decreased the wear factors especially at the highest doses. Also, even though all artificially aged materials showed a worse wear behaviour, polyethylenes implanted with either N+ or He+ at the highest doses maintained a relatively good wear factor in comparison with the aged non-implanted material. The origins of these modifications are discussed according to the effects of ion implantation on the microstructure of the polymer.
Asunto(s)
Materiales Biocompatibles/química , Polietilenos/química , Reactivos de Enlaces Cruzados/química , Elasticidad , Fricción , Dureza , Calor , Iones , Lubrificación , Ensayo de Materiales , Estrés MecánicoRESUMEN
Highly cross-linked polyethylenes (HXLPEs) have been incorporated into the hip replacement armamentarium based on their improved wear resistance. However, two different methods of thermal treatment separate the orthopedic community as strategies to control potential long-term oxidation, and controversy remains with problems in the long-term use of acetabular liners (long-term oxidation, rim fracture after impingement, etc.). Meanwhile, the mechanical properties of HXLPEs that may alleviate these problems are still unclear. On the other hand, HXLPEs are scarcely used in knee replacements, as there exists concern about the probably reduced fatigue and fracture performances of these materials. Thus, our aim was to compare the effects of both thermal treatment regimes on mechanical properties and to associate these findings with the material microstructure. The fatigue behavior of annealed and remelted HXLPEs was characterized using short-term cyclic stress-strain, long-term fatigue, and fatigue crack propagation tests. On the other hand, impact tests, tensile experiments, and the J-integral multispecimen method allowed us to assess toughness. Microstructure features such as crosslink density, crystallinity percentage, and lamellar thickness were investigated by swelling measurements, differential scanning calorimetry, and transmission electron microscopy, respectively. This study confirms that annealing preserves mechanical properties better than remelting from both fatigue and fracture resistance points of view, and it remarks that a suitable selection of irradiation and stabilization conditions is needed to achieve optimal mechanical performances of ultra high molecular weight polyethylenes for each specific total joint replacement.
Asunto(s)
Materiales Biocompatibles/química , Prótesis Articulares , Polietilenos/química , Calor , Humanos , Polietilenos/efectos de la radiación , Estrés Mecánico , Resistencia a la TracciónRESUMEN
The choice of necessary stent properties depends mainly on the length of the stenosis and degree of occlusion. So a stent design with variable radial stiffness along its longitudinal axis would be a good option. The design proposed corresponds to a tube-based stent with closed diamond-shaped cells made from a NiTi alloy. By acting independently on different geometric factors, variable geometries can be obtained with different radial force reactions. A design adjustment according to specific requirements, in order to get a better fit to ill-duct and reduces complications, is possible. A parametric analysis using finite element has been conducted to determine the influence of slot length, number of circumferential slots, tube thickness and shape-factor on stent mechanical behavior, which allow eliminating the need for extensive experimental work and knowing and quantifying the influence of those factors. The results of finite element simulations have been used, by means of least-squares fit techniques, to obtain analytical expressions for the main mechanical characteristics of the stent (Chronic Expansive Radial Force and Radial Compression Resistance) in terms of the different geometrical factors. This allows the stent geometry to be customized without launching an iterative and costly process of modeling and simulation for each case.
Asunto(s)
Diseño de Prótesis , Stents , Constricción Patológica , Análisis de Elementos Finitos , Fenómenos MecánicosRESUMEN
Electron beam irradiation at doses below 150 kGy is a widely used technique to obtain highly crosslinked ultra-high-molecular-weight polyethylene (UHMWPE). Its current use in total joint replacement components may improve wear resistance and decrease UHMWPE particle debris. However, currently used post-irradiation thermal treatments, which aim to decrease the free radicals within the material, introduce microstructural changes that affect UHMWPE mechanical properties, particularly the fatigue strength. This influence may be crucial in total knee replacements, where fatigue-related damage limits the lifespan of the prosthesis. Therefore, more studies are required to understand UHMWPE fatigue after current crosslinking protocols. This study was planned to evaluate the influence of UHMWPE remelting after irradiation on the material fatigue resistance. The remelting was achieved at 150 degrees C for 2 h on UHMWPE previously irradiated at 50, 100, and 150 kGy. Fatigue evaluation included short-term tests under cyclic tensile stress with zero load ratio, R = 0, and 1 Hz. In addition, stress-life testing was performed using 12% yield as the criterion for failure. Near-threshold fatigue crack propagation experiments were also performed at a frequency of 5 Hz, and crack length was measured in nonthermally treated and remelted irradiated UHMWPE. Crystallinity percentage was calculated from DSC measurements. The results pointed out that irradiation positively contributed to total life analysis, but the further remelting process decreased the flaw initiation resistance. On the other hand, both processes negatively affected the fatigue resistance of notched components. From a clinical point of view, the results suggest that the material fatigue behavior should be carefully studied in new UHMWPE to avoid changes related to material processing.
Asunto(s)
Electrones , Polietilenos/efectos de la radiación , Falla de Prótesis , Materiales Biocompatibles/química , Materiales Biocompatibles/efectos de la radiación , Prótesis de la Rodilla , Ensayo de Materiales , Polietilenos/química , Estrés Mecánico , Propiedades de Superficie , Resistencia a la TracciónRESUMEN
We have designed, manufactured and evaluated a prototype of a new stent based on the superelasticity of the NiTi alloy for colon obstruction, which is the first clinical manifestation of colorectal cancer in up to 29% of cases. The stent is auto-expandable diamond cell type, manufactured from a NiTi tube with 4.5 mm in diameter, in which longitudinal grooves were performed by cutting laser technique. The expansion process to the final shape was made by deformation in the martensite state. The stent reaches a high free expansion rate of 7, with a longitudinal variable radial strength and a bell-shaped profile in the extremes in order to avoid migration. Finite elements models were used for simulating the mechanical behaviour. The prototype was evaluated by implantation in a colon stenosis model of 6 mm in diameter performed in a porcine speciment. The stent gave a good deployment, fixation and capability to open the gap up to 15 mm in diameter.
Asunto(s)
Materiales Biocompatibles/química , Enfermedades del Colon/cirugía , Obstrucción Intestinal/cirugía , Níquel/química , Stents , Titanio/química , Animales , Materiales Biocompatibles/análisis , Diseño Asistido por Computadora , Análisis de Falla de Equipo , Ensayo de Materiales , Modelos Teóricos , Níquel/análisis , Diseño de Prótesis , Porcinos , Titanio/análisis , Resultado del TratamientoRESUMEN
We studied non-implanted ultra-high molecular weight polyethylene (UHMWPE) knee components, that were gamma-sterilized in air and shelf-aged during 6 and 7 years, to explore fracture mechanisms in the degraded material. Prior to the fractography study, we assessed degradation through FTIR and optical density measurements in microtome sections. Then, we morphologically compared the surface fractography of degraded, oxidized UHMWPE with that of non-degraded material in specimens from the same implants, fractured after uniaxial tensile tests in standardized samples. Fractography surface analysis clearly differentiated the behavior of non-degraded and degraded UHMWPE. Failure in non-degraded samples occurred through craze formation, followed by fast crack propagation. Quantitative fracture analysis suggested a ductile behavior. This was supported by the identified local plasticity in the region close to the initial flaw. In the fractography surface analysis of degraded material, a fibrillar behavior was observed. A heterogeneous domain structure appeared, showing different coarseness with multiple void sizes. Our results, based on mechanical measurements and fracture morphology in the subsurface region, confirmed the embrittlement of oxidized UHMWPE.
Asunto(s)
Materiales Biocompatibles/química , Sustitutos de Huesos/química , Rayos gamma , Prótesis de la Rodilla , Polietilenos/química , Polietilenos/efectos de la radiación , Sustitutos de Huesos/efectos de la radiación , Fracturas Óseas , Ensayo de Materiales , Propiedades de Superficie , Resistencia a la Tracción , Factores de TiempoRESUMEN
We studied the fracture surface evolution of ultra high molecular weight polyethylene (UHMWPE) specimens, manufactured from GUR 1050 compression moulded sheets, after gamma sterilisation in air followed by different aging times after thermal treatment at 120 degrees C. Degradation profiles were obtained by FTIR and DSC measurements after 0, 7, 14, 24 and 36h aging. We observed by SEM the morphology patterns at these aging times, in surface fractographies after uniaxial tensile test of standardised samples. The results pointed out clear differences between short and long aging times. At shorter times, 7h, the behaviour was similar to non-degraded UHMWPE, exhibiting ductile behaviour. At longer times, 24-36h, this thermal protocol provided a highly degraded zone in the subsurface, similar to the white band found after gamma irradiation in air followed by natural aging, although closer to the surface, at 150-200mum. The microstructure of this oxidation zone, similarly found in gamma irradiated samples shelf-aged for 6-7 years, although with different distribution of microvoids, was formed by fibrils, associated with embrittlement of the oxidised UHMWPE. In addition, the evolution of the oxidation index, the enthalpy content, the mechanical parameters, and the depth of the oxidation front deduced from the fractographies versus aging time showed that a changing behaviour in the degradation rate appeared at intermediate aging times.
Asunto(s)
Aire , Materiales Biocompatibles/química , Materiales Biocompatibles/efectos de la radiación , Rayos gamma , Calor , Polietilenos/química , Polietilenos/efectos de la radiación , Esterilización , Relación Dosis-Respuesta en la Radiación , Elasticidad , Ensayo de Materiales/métodos , Conformación Molecular , Polietilenos/análisis , Propiedades de Superficie , Resistencia a la Tracción , Factores de TiempoRESUMEN
Fatigue-related damage in UHMWPE is one of the main causes of long-term failure in total joint replacements. Crosslinking ultrahigh molecular weight polyethylene (UHMWPE) by gamma or electron-beam irradiation, in combination with prior or further thermal treatment, enhances its wear resistance against metallic components in total hip replacements, and eventually in knees. However, little information is available on the fatigue response of this modified UHMWPE. The objective of this study was to compare electron-beam-irradiated UHMWPE at 50, 100, and 150 kGy, with the well-known 25 kGy gamma-irradiated UHMWPE. Two different cyclic tests were performed under tensile stress, with a zero load ratio, R = 0. First, specimens were subjected to a sinusoidal load cycle at 1 Hz, which provided stress-life curves with the use of a failure criterion based on 12% yield strain. Second, specimens were tested under 50 load cycles at a displacement rate of 15 mm/min, which provided information about the evolution of secant modulus and plastic strain. The incubation period was also analyzed. DSC measurements were carried out to check the crystallization effect of irradiation. According to the results of fatigue resistance there was a crossover behavior between gamma- and electron-beam-irradiated UHMWPE regarding the applied stress. When the stress was higher than the crossover value, the fatigue resistance of gamma-irradiated samples was higher than electron-beam-irradiated ones. When the stress was lower, the fatigue behavior was the opposite. The crossover stress depended on the electron-beam-irradiation dose. The clinical relevance of this study lies in an improved knowledge of electron-beam-irradiated material under extreme mechanical circumstances, such as fatigue.
Asunto(s)
Materiales Biocompatibles , Análisis de Falla de Equipo , Ensayo de Materiales , Polietilenos , Materiales Biocompatibles/química , Materiales Biocompatibles/efectos de la radiación , Rastreo Diferencial de Calorimetría , Electrones , Rayos gamma , Humanos , Polietilenos/química , Polietilenos/efectos de la radiación , Estrés Mecánico , TemperaturaRESUMEN
We have designed and manufacture a prototype of a new anchoring system for soft bone tissue fixation, based on the superelasticity of the Ni-Ti alloy. The anchoring capability has been observed in femoral hips by radiographs. The performance of this new anchor have been measured by tensile experiments and contrasted with finite element model. The results point out that keeping the fixation capacity, the new configuration presents advantages concerning to a minor damaged volume bone, a reduction of the manufacture cost and a simpler insertion.
Asunto(s)
Análisis de Falla de Equipo/métodos , Fémur/cirugía , Ensayo de Materiales/métodos , Níquel , Técnicas de Sutura/instrumentación , Titanio , Fuerza Compresiva , Simulación por Computador , Elasticidad , Diseño de Equipo , Fémur/diagnóstico por imagen , Fémur/fisiopatología , Análisis de Elementos Finitos , Humanos , Fijadores Internos , Modelos Biológicos , Radiografía , Sensibilidad y Especificidad , Estrés Mecánico , Suturas , Resistencia a la TracciónRESUMEN
In this review we have evaluated the state of the art of CNT/UHMWPE and graphene/UHMWPE composites from four different points of view: mechanical properties, chemical stability, wear resistance and biocompatibility. The performance of these new carbonaceous composites allows us to conclude that these materials have overcome a first step on the way to developing into an alternative to the current HXLPEs used in orthopedic applications.
Asunto(s)
Artroplastia/instrumentación , Grafito/química , Nanotubos de Carbono/química , Polietilenos/química , Células 3T3 , Animales , Antiinfecciosos/química , Artroplastia/métodos , Materiales Biocompatibles , Humanos , Ensayo de Materiales , Ratones , Nanotecnología/métodos , Ortopedia , Prótesis e Implantes , Estrés Mecánico , Propiedades de SuperficieRESUMEN
Highly crosslinked ultra high molecular weight polyethylenes (UHMWPE) stabilized by a remelting process or by the addition of an antioxidant are highly wear resistant and chemically stable. However, these polyethylenes currently used in total joint replacements suffer a loss of mechanical properties, especially in terms of fracture toughness. In this study we analyze the impact behavior of different polyethylenes using an instrumented double notch Izod test. The materials studied are three resins: GUR1050, GUR1020 with 0.1wt% of vitamin E, and MG003 with 0.1wt% of vitamin E. These resins were gamma irradiated at 90kGy, and pre and post-irradiation remelting processes were applied to GUR1050 for two different time periods. Microstructural data were determined by means of differential scanning calorimetry and transmission electron microscopy. Fractography carried out on the impact fracture surfaces and images obtained by scanning electron microscopy after etching indicated the existence of a fringe structure formed by consecutive ductile-brittle and brittle-ductile transitions, which is related to the appearance of discontinuities in the load-deflection curves. A correlation has been made of the macroscopic impact strength results and the molecular chain and microstructural characteristics of these aforementioned materials, with a view to designing future resins with improved impact resistance. The use of UHMWPE resins with low molecular weight or the application of a remelting treatment could contribute to obtain a better impact strength behavior.
Asunto(s)
Materiales Biocompatibles , Ensayo de Materiales , Fenómenos Mecánicos , Polietilenos , Rayos gamma , Propiedades de SuperficieRESUMEN
A novel, vitamin E-stabilized, medical grade ultra-high molecular polyethylene, MG003 (DSM Biomedical; The Netherlands), has been very recently introduced for use in total joint replacements. This homopolymer resin features average molecular weight similar to that of conventional GUR 1050 resin (5.5-6*10(6)g/mol), but a higher degree of linearity. The aim of this study was to characterize the microstructure, thermal and thermooxidation properties as well as the mechanical behavior of this novel MG003 resin before and after gamma irradiation in air to 90 kGy. For this purpose, a combination of experimental techniques were performed including differential scanning calorimetry (DSC), thermogravimetry (TG), transmission electron microscopy (TEM), X-Ray Diffraction, electron paramagnetic resonance (EPR), and uniaxial tensile tests. As-consolidated MG003 materials exhibited higher crystalline contents (~62%), transition temperatures (~140 °C), crystal thickness (~36 nm), yield stress (~25 MPa) and elastic modulus (~400 MPa) than GUR 1050 controls (55%, 136 °C, 27 nm, 19 MPa, and 353 MPa, respectively). Irradiation produced similar changes in both MG003 and GUR 1050 materials, specifically increased crystallinity (63% and 60%, respectively), crystal thickness (39 nm and 30 nm), yield stress (27 MPa and 21 MPa), but, above of all, loss of elongation to breakage (down to 442 and 469%, respectively). Thermogravimetric and EPR results suggest comparable susceptibilities to oxidation for both MG003 and GUR 1050 polyethylenes. Based on the present findings, MG003 appears as a promising alternative medical grade polyethylene and it may satisfactorily contribute to the performance of total joint replacements.
Asunto(s)
Polietilenos/química , Vitamina E/química , Resinas Acrílicas/química , Rastreo Diferencial de Calorimetría , Módulo de Elasticidad , Espectroscopía de Resonancia por Spin del Electrón , Radicales Libres/química , Rayos gamma , Microscopía Electrónica de Transmisión , Oxidación-Reducción , Estrés Mecánico , Resistencia a la Tracción , Termogravimetría , Temperatura de Transición , Difracción de Rayos XRESUMEN
Ultrahigh molecular weight polyethylenes (UHMWPE) showing wear resistance, oxidative stability and good mechanical performance go on being a relevant research area in biomaterials for total joint replacements, where fatigue happens to be a recurrent damage mode that needs to be investigated. While crack propagation lifetime has been extensively studied, fatigue initiation data are scarcely offered in the literature, often due to the higher costs implied in the experimental programs. Moreover, their analysis is not always suitable to obtain reliable guidance. Different deterministic and probabilistic methods, generally resting on empirical bases have been previously used to analyze the fatigue initiation data. In this work, the probabilistic Weibull regression model of Castillo et al., based on both physical and statistical conditions, such as weakest link principle and the necessary compatibility between life-time and stress range distributions, is applied for the first time in the assessment of fatigue results of polymers, particularly to highly crosslinked UHMWPEs (HXLPEs). Accordingly, different published experimental data corresponding to HXLPE stabilized by thermal treatments and with α-tocopherol (vitamin E) are re-analyzed. Additional data are incorporated to assess the influence of notched HXLPE on fatigue performance. New conclusions are drawn from this revision.
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Modelos Estadísticos , Polietilenos/química , Falla de Prótesis , Antioxidantes/química , Análisis de los Mínimos Cuadrados , Ensayo de Materiales , Falla de Prótesis/efectos de la radiación , Estrés Mecánico , Temperatura , Factores de Tiempo , Tocoferoles/químicaRESUMEN
Development of intrinsically antibacterial surfaces is of key importance in the context of prostheses used in orthopedic surgery. This work presents a thorough study of several plasma-based coatings that may be used with this functionality: diamond-like carbon (DLC), fluorine-doped DLC (F-DLC), and a high-fluorine-content-carbon-fluor polymer (CF(X)). The coatings were obtained by a radio-frequency plasma-assisted deposition on ultra high molecular weight polyethylene (UHMWPE) samples and physicochemical properties of the coated surfaces were correlated with their antibacterial performance against collection and clinical Staphylococcus aureus and Staphylococcus epidermidis strains. The fluorine content and the relative amount of C-C and C-F bonds were controlled by X-ray photoelectron spectroscopy, and hydrophobicity and surface tension by contact angle measurements. Surface roughness was studied by Atomic Force Microscopy. Additional nanoidentation studies were performed for DLC and F-DLC coatings. Unpaired t test and regression linear models evaluated the adherence of S. aureus and S. epidermidis on raw and coated UHMWPE samples. Comparing with UHMWPE, DLC/UHMWPE was the least adherent surface with independence of the bacterial species, finding significant reductions (p ≤ 0.001) for nine staphylococci strains. Bacterial adherence was also significantly reduced in F-DLC/ UHMWPE and CFx/UHMWPE for six strains.
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Adhesión Bacteriana/efectos de los fármacos , Materiales Biocompatibles Revestidos/farmacología , Diamante/farmacología , Polietilenos/química , Staphylococcus aureus/citología , Staphylococcus epidermidis/citología , Módulo de Elasticidad/efectos de los fármacos , Dureza/efectos de los fármacos , Hidrocarburos Yodados/química , Pruebas de Sensibilidad Microbiana , Microscopía de Fuerza Atómica , Nanotecnología , Espectroscopía de Fotoelectrones , Análisis de Regresión , Staphylococcus aureus/efectos de los fármacos , Staphylococcus epidermidis/efectos de los fármacos , Tensión Superficial/efectos de los fármacos , Humectabilidad/efectos de los fármacosRESUMEN
There is an acknowledged need for shaping 3-D scaffolds with adequate porosity and mechanical properties for biomedical applications. The mechanical properties under static and cyclic compressive testing of dense and designed porous architecture bioceramic scaffolds based on the biphasic calcium phosphate (BCP) systems and BCP-agarose systems have been evaluated. The dense and designed porous architecture scaffolds in BCP systems exhibited a brittle behaviour. Agarose, a biocompatible and biodegradable hydrogel, has been used to shape designed architecture ceramic-agarose scaffolds following a low-temperature shaping method. Agarose conferred toughness, ductility and a rubbery consistency for strains of up to 60% of in ceramic BCP-agarose systems. This combination of ceramic and organic matrix helps to avoid the inherent brittleness of the bioceramic and enhances the compression resistance of hydrogel. The presence of mechanical hysteresis, permanent deformation after the first cycle and recovery of the master monotonous curve indicate a Mullins-like effect such as that observed in carbon-filled rubber systems. We report this type of mechanical behaviour, the Mullins effect, for the first time in bioceramics and bioceramic-agarose systems.
Asunto(s)
Regeneración Ósea/efectos de los fármacos , Fosfatos de Calcio/farmacología , Fuerza Compresiva/efectos de los fármacos , Ensayo de Materiales , Sefarosa/farmacología , Andamios del Tejido/química , Fosfatos de Calcio/química , Microscopía Electrónica de Rastreo , Tamaño de la Partícula , Porosidad/efectos de los fármacos , Temperatura , AguaRESUMEN
Sterilization with ethylene oxide (EO) and gas plasma (GP) are well-known methods applied to ultra-high molecular weight polyethylene (UHMWPE) surfaces in the belief that they prevent major material changes caused by gamma irradiation. However, the influence of these surface sterilization methods on bacterial adherence to UHMWPE is unknown. UHMWPE samples with various degrees of roughness (0.3, 0.8 and 2.0 µm) were sterilized with either GP or EO. The variations in hydrophobicity, surface free energy and surface functional groups were investigated before and after sterilization. Sterilized samples were incubated with either Staphylococcus aureus or Staphylococcus epidermidis in order to study bacterial adherence to these materials. Fewer bacteria adhered to UHMWPE after sterilization with EO than after sterilization with GP, especially to the smoothest surfaces. No changes in chemical composition of the UHMWPE surface due to sterilization were observed using X-ray photoemission spectroscopy analysis. The decreased bacterial adherence to UHMWPE found at the smoothest surfaces after sterilization with EO was not directly related to changes in chemical composition. Increased bacterial adherence to rougher surfaces was associated with increased polar surface energy of EO-sterilized surfaces.