RESUMEN
BACKGROUND: The investigation of UV-induced fluorescence imaging coupled with machine learning was conducted to non-destructively detect the total volatile basic nitrogen (TVB-N) of frozen-whole-round tilapia (FWRT) during freezing and thawing. The UV-induced fluorescence images of FWRT at the wavelength of 365 nm were acquired by self-developed fluorescence image acquisition system. In total, 169 color and texture features based on RGB, hue-saturation-intensity and L*a*b* color spaces and gray level co-occurrence matrix were extracted, respectively. Successive projections algorithm (SPA) was employed to select the optimal 16 features to achieve feature dimension reduction modeling. With full and extracted features as input, the models of partial least squares regression (PLSR), least-squares support vector machine (LSSVM) and convolutional neural network (CNN) were established for TVB-N prediction. RESULTS: Results indicated that the full features-based CNN performed better than SPA based prediction models (SPA-PLSR and SPA-LSSVM). The CNN model was determined to be the optimal with an RP2 value of 0.9779, RMSEP value of 1.1502 × 10-2 g N kg-1 and RPD value of 6.721 for TVB-N content predictiin. CONCLUSION: The CNN method based on UV fluorescence imaging technology has potential for quality and safety detection of FWRT. © 2023 Society of Chemical Industry.
Asunto(s)
Tilapia , Animales , Nitrógeno , Congelación , Redes Neurales de la Computación , Algoritmos , Análisis de los Mínimos CuadradosRESUMEN
The surface of carp is easily damaged during the descaling process, which jeopardizes the quality and safety of carp products. Damage recognition realized by manual detection is an important factor restricting the automation in the pretreatment. For the commonly used methods of mechanical and water-jet descaling, damage area recognition according to the hyperspectral data was proposed. Two discrimination models, including decision tree (DT) and self-organizing feature mapping (SOM), were established to recognize the damaged and normal descaling area with the average spectral value. The damage-discrimination model based on DT was determined to be the optimal one, which possessed the best model performance (accuracy = 96.7%, sensitivity = 96.7%, specificity = 96.7%, F1-score = 96.7%). Considering the efficiency and precision of damage-area recognition and visualization, the principal component analysis (PCA) combined with pixel values statistical analysis was used to reduce the dimension of hyperspectral images at the image level. Through statistical analysis, the value 0 was used as the threshold to distinguish the normal area and the damaged area in the PC image to achieve preliminary segmentation. Then, the spectral values of the initially discriminated damage area were input into the DT discrimination model to realize the final discriminant of damaged area. On this basis, the position information of the damaged area could be used to realize the visualization. The final visualization maps for mechanical and water-jet descaling damage were obtained by image morphology processing. The average recognition accuracy can reach 94.9% and 90.3%, respectively. The results revealed that the hyperspectral imaging technique has great potential to recognize the carp damage area nondestructively and accurately under descaling processing. PRACTICAL APPLICATION: This study demonstrated that hyperspectral imaging technique can realize the carp damage area detection nondestructively and accurately under descaling processing. With the advantages of nondestructive and rapid, hyperspectral imaging system and the method can be widely expanded and applied to the quality detection of other freshwater fish pretreatment.