Zebu cattle fed dry distiller's grain or cottonseed meal had greater nitrogen utilization efficiency than non-supplemented animals.

This study evaluated intake, apparent digestibility, ruminal parameters, nitrogen balance, and microbial protein synthesis in grazing beef cattle fed a mineral mix or combination of two coproducts (cottonseed meal and dried distiller's grains (DDG)) during the wet season. Urochloa brizantha cv. Marandu pastures were managed under continuous stocking to maintain a fixed grazing height of 25 cm using put-and-take methodology. Eight rumen cannulated Nellore steers were used to evaluate the different supplementation strategies. The experiment was composed of four treatments: (1) mineral mixed (MM; ad libitum); (2) energy-protein supplement using corn grain (energy) and cottonseed meal (protein; CS); (3) energy-protein supplement with 50% of the cottonseed meal replaced by DDG (50DDG); and (4) energy-protein supplement with 100% of the cottonseed meal replaced by DDG (100DDG). Except for MM, all supplements were supplied at a level of 0.3% of body weight (BW). A double Latin square was the experimental design performed, with eight cannulated animals, four treatments, across four experimental periods. There was a difference between dry matter and nutrient intake among treatments. The nitrogen balance was different between MM and the other treatments. There was a linear decrease in the rumen ammonia nitrogen levels under CS, 50DDG, and 100DDG. There were no treatment effects on the other parameters evaluated (P ≥ 0.10). Replacing the protein source in the supplement composition did not affect the metabolic parameters and the microbial protein synthesis. Supplementation at a rate of 0.3% BW, compared to MM supplementation, increased the nitrogen utilization efficiency in grazing cattle.

Effect of pasture management on enteric methane emissions from goats.

The effect of pasture management on CH4 emissions was investigated from goats in a tropical climate. Two experiments were conducted in a "Tanzania Guinea grass" (Panicum maximum Jacq.) pasture to assess enteric CH4 production in a completely randomized design. Emissions from light, moderate, and heavy grazing intensities were analyzed in the first experiment, and variations between grazing days were explored in the second experiment. Grazing intensity was defined as 2.4, 1.6, and 0.8 post-grazing leaf area index. Pasture management employed intermittent grazing with variable stocking rate using Anglo Nubian female adult goats. SF6 tracer gas technique was used to measure CH4 production. Grazing intensity was not found to affect CH4 emissions per animal, dry matter forage intake (DMI), and gross energy (GE) intake. However, the second experiment showed that CH4 production was influenced by the grazing day. CH4 emissions were 18.1 g day-1, and the variables were 0.88 g kg-1 of metabolic weight, 17.45 g kg-1 of DMI, and 5.5% of GE. CH4 production increased linearly with the grazing day, possibly reflecting a reduction in forage quality. These findings suggest that the day of occupation in intermittent grazing has a greater effect on CH4 emissions than that by grazing intensity and that a single day grazing of Tanzania Guinea grass could mitigate CH4 emissions.

Economic evaluation from beef cattle production industry with intensification in Brazil's tropical pastures.

This study aimed to evaluate different scenarios (year, supplementation level) about economic results of beef cattle production during rearing and finishing phase in Brazilian's tropical pastures. Four scenarios were evaluated in combination with fourteen supplements, and it was originated from some research developed inside Forage Crops and Grasslands section from São Paulo State University among years 2011 and 2014. The economic evaluation was analyzed by operating cost, total operational costs, gross revenue, operating profit, and financial net income. Besides profitability, internal rate of return (IRR), benefit/cost ratio (B:C), and simple payback period (SPP) were calculated too. During rearing phase, the best result was observed for scenario 2 (2012), supplement 3.2 (mineral mix) with values of 11 cycles, 26.3%, 9.30%, and 0.39 for SPP, profitability, IRR, and B:C ratio, respectively. Already to finishing phase, the best scenario was 3 (2013), supplement 10 (multiple supplement with supplementation level equal 1.0% body weight), which obtained 4 cycles, 68.7%, 27.00%, and 2.34 for the same variables above mentioned. Results were consistent being that higher IRR and profitability occurred when using low supplementation level. Hence, the economic responses from different scenarios (years and supplements) can alter the final livestock farm financial statement.

How do methane rates vary with soil moisture and compaction, N compound and rate, and dung addition in a tropical soil?

Soil moisture and compaction, and source of N and bovine urine can reduce methane (CH4) rates from agricultural soils. However, the magnitude of the effect is unknown in tropical soil under different conditions, as well as the potential of different urine-N concentration, volume, and sources of N in such an effect. This study aimed to investigate the effects of different soil conditions (moist, dry, compacted, moist-dung, moist-dung-compacted), N concentration in urine (2.5, 5.0, 10.0, and 15.0 g N L-1), volume of urine (25, 50, 100, and 200 ml kg-1 dry soil), and source of N (ammonium, nitrate, and urea) on CH4 emissions. A tropical Ferralsol soil from marandu-grass pasture was incubated during 106 days and the CH4 concentration determined by gas chromatography. The CH4 rates varied significantly according to the soil conditions when manipulated the urine-N (p < 0.01) and averaged 0.75, - 0.50, 1.14, 6.23, and 8.17 µg C-CH4 m-2 h-1for the moist, dry, compacted, moist-dung, and moist-dung-compacted soil, respectively, and, not responded to the level of N (p = 0.73) averaging 2.57 µg C-CH4 m-2 h-1. When evaluated, the volumes of urine cumulative CH4 averages were - 0.52, - 1.24, - 0.88, 14.48, and 18.56 µg C-CH4 m-2 h-1 for the moist, dry, compacted, moist-dung, and moist-dung-compacted, respectively. Soils were affected by soil treatments (p < 0.001) but not by urine volumes (p = 0.30). The source of N did not influence the CH4 rates (p = 0.1) averaging 0.88, - 1.26, and - 1.19 µg C-CH4 m-2 h-1 respectively, for urea, nitrate, and ammonium. The CH4 fluxes in tropical Ferralsols are controlled by the soil characteristics and dung addition.

Mineral salt intake effects on faecal-N concentration and the volume and composition of beef cattle urine.

The effect of mineral salts on water ingestion and urine volume in cattle has been extensively studied. However, recently, this effect has been investigated as a potential mitigator of environmental aspects related to the nitrogen (N) cycle, such as nitrate (NO3-) lixiviation, ammonia (NH3) volatilisation, and nitrous oxide (N2O) emissions. The effect of mineral salts, particularly sodium chloride (NaCl), on urine-N concentration, urine volume, the proportion of N compounds in the urine, and faecal-N concentration has not yet been explored in field conditions with respect to environmental aspects of beef cattle production. The present study investigated the effect of dietary mineral salt rates on these parameters. A Latin square (5 × 5) experimental design was utilised with five concentrations of mineral salts in the diet: 0.0, 2.0, 4.0, 6.0, and 8.0 g based on dry matter (DM) ingestion (g/kg DM). The nitrogen concentration in the urine and urine volume increased linearly. The total N excreted (g/day) via urine did not vary with increasing mineral salt concentrations. When evaluated, the N compounds of urine (urea-N, allantoin-N, and hippuric acid-N) also reacted to the increased mineral salt concentrations, while creatinine-N did not. Urea-N, allantoin-N, and hippuric acid-N linearly increased their proportions in total N-urine. The N concentration in faeces was not affected by mineral salt concentrations. The urine volume, concentration of N, and proportion of N compounds in the urine affected N2O emissions and NH3 volatilisation. Therefore, mineral salt utilisation may be an option for mitigating N pollution from beef cattle, especially for grasslands in tropical countries.

The nutritional interrelationship between the growing and finishing phases in crossbred cattle raised in a tropical system.

This study evaluated the nutritional interrelationship between the growing and finishing phases of crossbred cattle in determining their performance. One hundred and eight animals were used (8 months old, body weight [BW] 211 ± 20 kg). During the dry season, the animals received one of the following supplements: a mineral plus urea supplement (ad libitum, MSD), a protein supplement (1 g/kg BW per day, PR1), or a protein-energy supplement (5 g/kg BW per day, PE). During the rainy season, the animals received one of the following supplements: a mineral without urea supplement (ad libitum, MSR), a protein supplement (1 g/kg BW per day, PR2), or PE (5 g/kg BW per day). The experimental design was completely randomized using a 3 × 3 factorial scheme (for the rainy season) and a 3 × 3 × 2 factorial scheme (for the finishing phase). The supplementation and finishing systems were considered to be the treatments, and the animals were considered to be the experimental units. Dry season supplementation did not affect the average daily gain (ADG) during the rainy season (P = 0.12) or the finishing phase (P = 0.73). An increase in the level of rainy season supplementation reduced ADG by 12% during the finishing phase (P < 0.06). Providing PE during the dry and rainy seasons led to the animals being slaughtered 17 (P = 0.06) and 30 (P < 0.01) days earlier, respectively. Our results indicate that supplementation during the dry season (under poor-quality pasture conditions) does not affect the performance during the rainy season or the finishing phase. Furthermore, while providing PE during the rainy season can reduce ADG during finishing, the higher BW at the beginning of the finishing phase is sufficient to reduce the time of the finishing period.

Prediction of beef color using time-domain nuclear magnetic resonance (TD-NMR) relaxometry data and multivariate analyses.

Time-domain nuclear magnetic resonance and chemometrics were used to predict color parameters, such as lightness (L*), redness (a*), and yellowness (b*) of beef (Longissimus dorsi muscle) samples. Analyzing the relaxation decays with multivariate models performed with partial least-squares regression, color quality parameters were predicted. The partial least-squares models showed low errors independent of the sample size, indicating the potentiality of the method. Minced procedure and weighing were not necessary to improve the predictive performance of the models. The reduction of transverse relaxation time (T2 ) measured by Carr-Purcell-Meiboom-Gill pulse sequence in darker beef in comparison with lighter ones can be explained by the lower relaxivity Fe2+ present in deoxymyoglobin and oxymyoglobin (red beef) to the higher relaxivity of Fe3+ present in metmyoglobin (brown beef). These results point that time-domain nuclear magnetic resonance spectroscopy can become a useful tool for quality assessment of beef cattle on bulk of the sample and through-packages, because this technique is also widely applied to measure sensorial parameters, such as flavor, juiciness and tenderness, and physicochemical parameters, cooking loss, fat and moisture content, and instrumental tenderness using Warner Bratzler shear force. Copyright © 2016 John Wiley & Sons, Ltd.

Using sentinel-2 satellite images and machine learning algorithms to predict tropical pasture forage mass, crude protein, and fiber content.

Grasslands cover approximately 24% of the Earth's surface and are the main feed source for cattle and other ruminants. Sustainable and efficient grazing systems require regular monitoring of the quantity and nutritive value of pastures. This study demonstrates the potential of estimating pasture leaf forage mass (FM), crude protein (CP) and fiber content of tropical pastures using Sentinel-2 satellite images and machine learning algorithms. Field datasets and satellite images were assessed from an experimental area of Marandu palisade grass (Urochloa brizantha sny. Brachiaria brizantha) pastures, with or without nitrogen fertilization, and managed under continuous stocking during the pasture growing season from 2016 to 2020. Models based on support vector regression (SVR) and random forest (RF) machine-learning algorithms were developed using meteorological data, spectral reflectance, and vegetation indices (VI) as input features. In general, SVR slightly outperformed the RF models. The best predictive models to estimate FM were those with VI combined with meteorological data. For CP and fiber content, the best predictions were achieved using a combination of spectral bands and meteorological data, resulting in R2 of 0.66 and 0.57, and RMSPE of 0.03 and 0.04 g/g dry matter. Our results have promising potential to improve precision feeding technologies and decision support tools for efficient grazing management.

The Impact of Mineral and Energy Supplementation and Phytogenic Compounds on Rumen Microbial Diversity and Nitrogen Utilization in Grazing Beef Cattle.

The objective of this study was to evaluate the effect of the addition of a phytogenic compound blend (PHA) containing hydrolyzable tannins, carvacrol, and cinnamaldehyde oil to mineral salt or energy supplementation on the rumen microbiota and nitrogen metabolism of grazing Nellore cattle. Eight castrated Nellore steers were distributed in a double-Latin-square 4 × 4 design, with a 2 × 2 factorial arrangement (two types of supplements with or without the addition of the PHA), as follows: energy supplement without the PHA addition (EW); energy supplement with the PHA addition (EPHA); mineral supplement without the addition of the PHA (MW); mineral supplement with the PHA addition (MPHA). Steers that received supplements with the PHA have a lower ruminal proportion of valerate (with the PHA, 1.06%; without the PHA, 1.15%), a lower ruminal abundance of Verrucomicrobia, and a tendency for lower DM digestibility (with the PHA, 62.8%; without the PHA, 64.8%). Energy supplements allowed for higher ammonia concentrations (+2.28 mg of NH3-N/dL), increased the propionate proportion (+0.29% of total VFA), and had a higher ruminal abundance of Proteobacteria and Spirochaetae phyla in the rumen. The PHA addition in the supplement did not improve nitrogen retention, reduced the ruminal proportion of valerate, and had a negative impact on both the total dry-matter digestibility and the abundance of several ruminal bacterial groups belonging to the Firmicutes and Verrucomicrobia phyla.

Efficiency of Amino Acid Utilization in Nellore Cattle Grazing Low-Quality Forage Supplemented with Different Sources of Nitrogen.

This study aimed to evaluate the effects of supplementation with non-protein nitrogen (NPN) or ruminal undegradable protein (RUP) on intake, digestibility, and amino acid (AA) use efficiency of Nellore cattle grazing during the dry season. Eight Nellore steers (12 ± 2 months old) were used in quadruplicate Latin squares (2 × 2). The animals were placed on Urochloa brizantha cv. Xaraés under continuous grazing. The treatments included the following: (1) urea supplementation (NPN) and (2) supplementation of corn gluten meal 60 (CGM, RUP). Animals supplemented with CGM showed higher intakes of dry matter (DM) supplement, total AA, essential AA, and individual AA. The supplementation did not affect the total AA digestibility, total AA flux, and the AA fluxes of microbial origin and RUP from the diet (p > 0.05). The ruminal microorganism origin flux of total AA to the duodenum was 44.5% and 52.7% for animals supplemented with NPN and CGM, respectively. Animals supplemented with CGM showed an increase in blood concentrations of isoleucine (+19.09 µmol/L), cystine (+27.29 µmol/L), and albumin (+0.11 g/dL) (p < 0.05), but this increase was not accompanied by an improvement in N use efficiency of steers (p > 0.05). RUP supplementation via CGM can be an efficient nutritional strategy to enhance the intake and absorption of AA by Nellore cattle grazing low-quality forage during the dry season.

N-fertilization of tropical pastures improves performance but not methane emission of Nellore growing bulls.

Grazing management and N-fertilizer have been reported to improve tropical forage productivity and quality, however, their effect on methane emission of grazing animals remains uncertain. Therefore, this study aimed to assess the effects of increasing application rates of nitrogen (N) fertilization of Marandu palisadegrass under continuous stocking on intake, digestibility, nitrogen balance, and enteric methane emissions of Nellore growing bulls. We hypothesized that changes in the forage nutritive value caused by N fertilization of pastures combined with adequate grazing management (e.g., greater crude protein [CP] and digestibility) would lead to an increase in animal productivity (e.g., greater average daily gain [ADG] and gain per area), and then, to a decrease in methane emission intensity. Treatments consisted of different annual application rates of nitrogen fertilization: 0, 75, and 150 kg N/ha using ammonium nitrate (32% N) as the nitrogen source. The experimental design was completely randomized, with three treatments and four replications (12 paddocks). Intake, digestibility, N balance, and methane emissions were measured in eight animals per treatment. CP intake, digestibility and N balance increased linearly with the increase in N fertilization (P < 0.05). In addition, stocking rate (SR) and ADG linearly increased from 1.75 animal unit (AU = 450 kg)/ha and 0.62 kg/d (0 kg N/ha) to 3.75 AU/ha and 0.82 kg/d (150 kg N/ha), respectively. Individual methane emissions nor methane emission intensity were affected by treatment with an average of 164.7 g/d and 199.7 g/kg ADG (P > 0.05). Annual N fertilization with ammonium nitrate between 75 and 150 kg N/ha in palisadegrass pastures under continuous stocking enhances animal performance per unit area yet not affecting neither methane production nor intensity.

The availability of nitrogen in the soil is one of the main factors that can affect plant growth and characteristics. Nitrogen fertilization is a tool to increase the efficiency in the productive responses of grazing beef cattle, reconciling the greater production per area, reduction of the production cycle, maximization of the use of nutrients, with the maintenance of the system sustainability by enhancing animal production indexes. The present study evaluated performance and methane production of Nellore growing bulls grazing continuously stocked Marandu palisadegrass fertilized with three rates of ammonium nitrate (0, 75, and 150 kg N/ha). Although nitrogen fertilization did not affect individual methane production, both stocking rate and average daily gain linearly increased as fertilization rate increased, thus increasing the beef productivity.

Effects of condensed tannins on greenhouse gas emissions and nitrogen dynamics from urine-treated grassland soil.

Condensed tannins are a potentially important treatment option to mitigate N2O (nitrous oxide) and affect carbon dioxide (CO2) and methane (CH4) emissions; however, their effect has been poorly assessed. Here, we quantified the emissions of N2O, CH4, and CO2, soil N mineralization, and nitrification with increasing doses of condensed tannins added to the urine of cattle raised on pasture. The experiment consisted of incubation with doses of 0%, 0.5%, and 1.0% of condensed tannins added directly to the collected urine. The experimental design was completely randomized. Greenhouse gas fluxes were quantified for four weeks using static chambers and gas chromatography. The addition of condensed tannins increased N2O emissions (P < 0.05), with total emissions averaging 95.84 mg N-N2O kg-1, 265.30 mg N-N2O kg-1, and 199.32 mg N-N2O kg-1 dry soil in the treatments with 0%, 0.5%, and 1% tannins, respectively. Methane emissions were reduced with the addition of tannins (P < 0.05), with total emissions of 8.84 g CH4 kg-1, 1.87 g CH4 kg-1, and 3.34 g CH4 kg-1 dry soil in the treatments with 0%, 0.5%, and 1% tannins, respectively. Soil respiration increased with the addition of condensed tannins (P < 0.05), with total emissions of 3.80 g CO2 kg-1, 6.93 g CO2 kg-1, and 5.87 g CO2 kg-1 in dry soil, in the treatments with 0%, 0.5%, and 1% tannins, respectively. The addition of condensed tannins reduced N mineralization and nitrification. We found evidence that the use of condensed tannins might not be a suitable option to mitigate N2O emissions. However, soil CH4 emissions can be abated. The increases in soil respiration suggest that tannins affect soil microorganisms, and the effects on CH4 and N2O could be related to the variation in the soil microbiome, which requires further clarification.

Modern livestock farming under tropical conditions using sensors in grazing systems.

The aim of this study was to evaluate a commercial sensor-a three-axis accelerometer-to predict animal behavior with a variety of conditions in tropical grazing systems. The sensor was positioned on the underjaw of young bulls to detect the animals' movements. A total of 22 animals were monitored in a grazing system, during both seasons (wet and dry), with different quality and quantity forage allowance. The machine learning (ML) methods used were random forest (RF), convolutional neural net and linear discriminant analysis; the metrics used to determine the best method were accuracy, Kappa coefficient, and a confusion matrix. After predicting animal behavior using the best ML method, a forecast for animal performance was developed using a mechanistic model: multiple linear regression to correlate intermediate average daily gain (iADG) observed versus iADG predicted. The best ML method yielded accuracy of 0.821 and Kappa coefficient of 0.704, was RF. From the forecast for animal performance, the Pearson correlation was 0.795 and the mean square error was 0.062. Hence, the commercial Ovi-bovi sensor, which is a three-axis accelerometer, can act as a powerful tool for predicting animal behavior in beef cattle production developed under a variety tropical grazing condition.

Citrus Pulp Replacing Corn in the Supplement Decreased Fibre Digestibility with No Impacts on Performance of Cattle Grazing Marandu Palisade Grass in the Wet-Dry Transition Period.

The wet-dry transition period brings unique challenges to cattle producers in the tropics as the forage quality starts to decrease and animal performance is negatively affected unless supplementation strategies are applied. Two experiments were conducted concomitantly to evaluate the performance and metabolic parameters of cattle supplemented with two different energy sources under a C4 tropical grass continuous grazing system in the wet-dry transition period. In experiment (exp) 1, the liveweight (LW) gain of 42 growing Bos indicus Nellore bulls allocated to 12 paddocks of Uruchloa brizantha cv. Marandu, in a completely randomized block design, was evaluated to compare corn and citrus pulp-based supplements offered at 0.5% LW. Metabolism was evaluated in exp 2 with eight rumen-cannulated Nellore steers in an incomplete replicated Latin square design (RLSD) 4 × 2 (steers × treatment) fed the same two treatments as in exp 1. No differences in animal performance were observed between corn or citrus pulp-based supplements. Rumen pH, ammonia nitrogen, and blood urea nitrogen were lower in the rumen of animals supplemented with citrus pulp. Despite this, there were no differences in total dry matter intake amongst the two energy sources. The neutral detergent fibre digestibility of the diet containing corn decreased, but it neither affected performance nor liveweight gain per area. The findings suggest that corn can be replaced by citrus pulp with neither detrimental effects on animal performance nor indirect effects on pastures productivity.

Production of fibrolytic enzymes by Aspergillus japonicus C03 using agro-industrial residues with potential application as additives in animal feed.

Solid-state fermentation obtained from different and low-cost carbon sources was evaluated to endocellulases and endoxylanases production by Aspergillus japonicus C03. Regarding the enzymatic production the highest levels were observed at 30 °C, using soy bran added to crushed corncob or wheat bran added to sugarcane bagasse, humidified with salt solutions, and incubated for 3 days (xylanase) or 6 days (cellulase) with 70% relative humidity. Peptone improved the xylanase and cellulase activities in 12 and 29%, respectively. The optimum temperature corresponded to 60 °C and 50-55 °C for xylanase and cellulase, respectively, both having 4.0 as optimum pH. Xylanase was fully stable up to 40 °C, which is close to the rumen temperature. The enzymes were stable in pH 4.0-7.0. Cu++ and Mn++ increased xylanase and cellulase activities by 10 and 64%, respectively. A. japonicus C03 xylanase was greatly stable in goat rumen fluid for 4 h during in vivo and in vitro experiments.

Effects of nitrogen fertilization on protein and carbohydrate fractions of Marandu palisadegrass.

The effects of nitrogen (N) fertilization levels on protein and carbohydrate fractions in Marandu palisadegrass pasture [Urochloa brizantha (Hochst. ex A. Rich.) R.D. Webster] were investigated in a pasture over five years. The experimental design was completely randomized with four levels of N (0, 90, 180, and 270 kg N ha-1, as urea) for five years, and with three replicates. The study was conducted in a continuously stocked pasture during the forage growing season (December to April) in a tropical region. The effects of N fertilization were similar across the five years. With increasing N fertilization, the concentrations of crude protein (CP) increased from 103 to 173 g kg-1 (P < 0.001), soluble fractions (Fraction A + B1) increased from 363 to 434 g kg-1 of total CP (P = 0.006); neutral detergent fiber (NDF) decreased from 609 to 556 g kg-1 (P = 0.037); indigestible NDF (P = 0.046), potentially degradable neutral detergent fiber (P = 0.037), and acid detergent fiber decreased (P = 0.05), and total digestible nutrient (TDN) increased (P < 0.001). Increasing N fertilization decreased the concentrations of Fraction C (P = 0.014) and total carbohydrates (P < 0.0001), and increased CP:organic matter digestibility (P < 0.01). Concentrations of neutral detergent fiber free of ash and protein (P = 0.003), indigestible neutral detergent fiber (P < 0.001), neutral detergent fiber potentially degradable (P = 0.11), CP (P < 0.001), Fraction A + B1 (P < 0.001), Fraction B2 (P < 0.001), Fraction B3 (P < 0.01), and non-structural carbohydrates differed (P < 0.001) across years. Therefore, N fertilization can be used to increase CP, soluble protein, and TDN.

Effects of Replacing Cottonseed Meal with Corn Dried Distillers' Grain on Ruminal Parameters, Performance, and Enteric Methane Emissions in Young Nellore Bulls Reared in Tropical Pastures.

Two experiments were conducted to evaluate the effect of replacing cottonseed meal with DDG on ruminal parameters, methane (CH4) emissions (Experiment 1), and animal performance (Experiment 2) of young Nellore bulls grazing Marandu grass during the rainy season. Four supplementation strategies were used in both experiments: (1) Mineral supplementation (MS); (2) conventional multiple supplement (energy/protein) with cottonseed meal and citrus pulp (CMS); (3) CMS with 50% cottonseed meal replaced by DDG (50DDG); and (4) CMS with 100% cottonseed meal replaced by DDG (100DDG). The 50DDG condition resulted in greater intake of dry matter (p = 0.033), organic matter (OM) (p = 0.050), forage (p = 0.035), and digestible OM (p = 0.031) than 100DDG. The supplemented animals presented greater final body weight (BW) and average daily gain than the animals consuming MS (p = 0.011), and lower pH, acetate, and acetate:propionate (p < 0.05). However, the treatments had no influence on stocking rate, gain per area, and enteric CH4 emissions (p > 0.05). Replacing cottonseed meal with DDG does not result in great variations in ruminal parameters, animal performance, and enteric CH4 emissions of grazing Nellore cattle during the rearing phase in the wet season. Both protein sources in 0.3% BW supplementation can be used to intensify beef cattle production in pastures.

Does the Effect of Replacing Cottonseed Meal with Dried Distiller's Grains on Nellore Bulls Finishing Phase Vary between Pasture and Feedlot?

The study aimed to evaluate the effect of replacing cottonseed meal by dried distiller's grains (DDG) in terms of efficiency in the productive aspects of beef cattle finishing in pasture versus feedlot. The experiment was conducted in a completely randomized design in a 2 × 3 factorial arrangement, with two production systems (pasture versus feedlot) and three supplements: CM, conventional supplement with cottonseed meal (CM) as a protein source; 50DDG: supplement with 50% replacement of CM by DDG; and 100DDG: 100% replacement. The effect of replacing CM by DDG on dry matter and nutrients intake and nutrients digestibility depends on the finishing system (p < 0.05). While in the pasture system animal consumed more nutrients in the CM, a greater intake was observed in the 100DDG in feedlot. The nutrients digestibility was lower in the pasture (p < 0.05). Animal performance and final body weight were higher in the feedlot (p < 0.0001), with averages of 1.57 kg/d and 566 kg of final body weight (FBW) for feedlot, and 0.99 kg/d and 504 kg FBW for pasture. The use of DDG does not change the animal performance finished in pasture or feedlot, and it is a viable alternative to replace conventional supplements in finishing phase in both systems in tropical environment.

Proteomic Analysis of Liver from Finishing Beef Cattle Supplemented with a Rumen-Protected B-Vitamin Blend and Hydroxy Trace Minerals.

Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways; however, their bioavailability is compromised in high-producing ruminant animals. So far, studies have mainly focused on the effects of these micronutrients on animal performance, but their use in a rumen-protected form and their impact on liver metabolism in finishing beef cattle is poorly known. We used a shotgun proteomic approach combined with biological network analyses to assess the effects of a rumen-protected B-vitamin blend, as well as those of hydroxy trace minerals, on the hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial body weight were randomly assigned to one of the following treatments: CTRL-inorganic trace minerals without supplementation of a protected vitamin B blend, or SUP-supplementation of hydroxy trace minerals and a protected vitamin B blend. All animals were fed the same amount of the experimental diet for 106 days, and liver biopsies were performed at the end of the experimental period. Supplemented animals showed 37 up-regulated proteins (p < 0.10), and the enrichment analysis revealed that these proteins were involved in protein folding (p = 0.04), mitochondrial respiratory chain complex I (p = 0.01) and IV (p = 0.01), chaperonin-containing T-complex 2 (p = 0.01), glutathione metabolism (p < 0.01), and other aspects linked to oxidative-stress responses. These results indicate that rumen-protected vitamin B and hydroxy trace mineral supplementation during the finishing phase alters the abundance of proteins associated with the electron transport chain and other oxidation-reduction pathways, boosting the production of reactive oxygen species, which appear to modulate proteins linked to oxidative-damage responses to maintain cellular homeostasis.

Nitrogen supply and rainfall affect ammonia emissions from dairy cattle excreta and urea applied on warm-climate pastures.

Cattle excreta and nitrogen (N) fertilizer deposited on tropical grasslands are important sources of ammonia (NH3 ) emission. We conducted three field trials (wet, intermediate, and dry conditions) to quantify NH3 emissions from urea fertilizer and simulated excretions of heifer urine and dung on warm-climate grasslands in Brazil. Heifer excreta were derived from pastures of palisadegrass [Urochloa brizantha (Hochst. ex A. Rich.) R. D. Webster 'Marandu'] under three forms of N supply (without or with N fertilization [0 or 150 kg N ha-1  yr-1 ] or mixed with forage peanut [Arachis pintoi 'Amarillo']). Cumulative NH3 -N emissions across rainfall conditions were 7.6-16.6% (mean, 11.7%) for urine, 1.4-2.9% (mean, 2.0%) for dung, and 11.2-20.5% (mean, 14.8%) for urea. Ammonia loss from urine was significantly greater than from dung under all rainfall conditions. Emission from urine and dung differed from those when urea was applied on palisadegrass. There were greater NH3 emissions from urine in the wetter times of the year. Heifer excreta from N-fertilized pasture had greater NH3 emission than excreta from the grass-legume mixture and unfertilized palisadegrass. Urea applied on palisadegrass presented greater NH3 emissions in wet rainfall conditions compared with dry conditions but did not differ from intermediate conditions. Our study showed that N-fertilized systems increase N losses as NH3 emission from excreta, and emissions from urea fertilizer must be included in this system. Heifer excreta and urea fertilizer deposited on warm-climate grasslands increased the NH3 emissions mainly under wet conditions.