Synergistic effect of Ficus-zero valent iron supported on adsorbents and Plantago major for chlorpyrifos phytoremediation from water.

Chlorpyrifos and the metabolite 3, 5, 6-trichloro-2-pyridinol (TCP) are widespread contamination of aquatic environments especially freshwater fish. The objectives of this study were to evaluate the contribution of using Ficus zero-valent iron nanoparticles supported on adsorbents (F-Fe0 ad) as green nanotechnology and Plantago major as phytoremediation for removing chlorpyrifos and degradation product TCP polluted water. The shapes of F-Fe0 were circular, with sizes from 2.46 nm to 11.49 nm. Wheat bran (WB) showed the highest extent of removal of chlorpyrifos, while Rice straw ash (RSA) showed the lowest extent of removal. F-Fe0 supported on adsorbents has demonstrated faster removal toward chlorpyrifos compared with tested adsorbents or F-Fe0. Chlorpyrifos was removed more quickly and effectively by P. major L. plus F-Fe0 supported on different adsorbents (nearly 100%) than that by P. major (43.76%) or F-Fe0 (81.69%). The degradation product TCP was more greatly accumulated in water treated with F-Fe0 than that P. major alone or F-Fe0 supported with adsorbents and combined with P. major. Furthermore, TCP significantly accumulated in P. major roots and leaves in the water treated with F-Fe0 supported with adsorbents plus P. major more than that in the P. major roots and leaves alone, this is attributed to the role of F-Fe0 adsorbents for the degradation of chlorpyrifos to TCP, Which strongly accumulated in the P. major roots and leaves. It can be concluded that the contribution of using F-Fe0 supported on adsorbents, especially WB as green nanotechnology and P. major as phytoremediation would be a major role for the complete removal of chlorpyrifos from the water with a significant reduction in the toxic degradation product TCP.

Synergistic use of Plantago major and effective microorganisms, EM1 to clean up the soil polluted with imidacloprid under laboratory and field condition.

Imidacloprid is known to induce soil pollution. Herein, we improved phytoremediation of soil contaminated with imidacoprid using Plantago major plus effective microorganisms (EM1) and peat-moss under laboratory conditions for 14-days and using P. major inoculated with EM1 in tomato field for 10-days. Concentration of imidacloprid in soil, roots and leaves was determined. Concentrations of Chlorophyll in leaves were also determined. Our results showed that lower imidacloprid degradation rate was observed in amended soil with peat-moss, while higher imidacloprid degradation rate was observed in soils vegetated with P. major, P. major inoculated by EM1, and P. major amended with peat-moss, respectively. However, degradation rate was high in the case of interaction between soil planted with P. major plus both of EM1 and peat-moss. Concentration of imidacloprid in P. major roots amended with EM1, peat-moss and both was significantly lower than that of P. major roots alone within 1-14 days of treatment. EM1 plus P. major and peat- moss plus P. major had increase of Chlorophyl content in leaves. Cultivation of P. major next to tomatoes crop and inoculated with EM-1 was found to be the most effective process for removing imidacloprid from the soil.

Green nano-phytoremediation and solubility improving agents for the remediation of chlorfenapyr contaminated soil and water.

Chlorfenapyr is a novel class of insecticide-miticide used for crop protection. It poses substantial risks to the reproductive ability of birds as well as environmental stability. This study was focused on the remediation of chlorfenapyr-polluted soil and water through the combined application of green nanotechnology, solubility improving agents and phytoremediation. An analysis using electron microscopy showed that the green synthesis resulted in circular ficus iron nanoparticles (F-Fe0) with diameters of 2.46 nm-11.49 nm, while the ipomoea-silver (Ip-Ag0) and brassica-silver nanoparticles (Br-Ag0) were circular, cubical, hexagonal, triangular and rod -like in shape with sizes ranging from 6.27 to 21.23 nm in IP-Ag0 and from 6.05 to 15.02 nm in Br-Ag0. After 24 h of treatment with F-Fe0, Ip-Ag0 and Br-Ag0 supported on activated charcoal (Ach), the chlorfenapyr in the aqueous solution was reduced to 86%, 79.70%, and 79.70%, respectively, compared to the 6.16% in aqueous solution. Moreover, after 24 h of treatment with Plantago major plus F-Fe0Ach, P. major plus Ip-Ag0Ach, and P. major plus Br-Ag0Ach, the chlorfenapyr in the aqueous solution was reduced to 93.7%, 91.30%, and 92.92%, respectively, as compared to the 69.27% in P. major. After four days of exposure, the percentage of chlorfenapyr degradation in the soil (i.e. control) only reached 12.40%,while the degradation rates were enhanced by 71.22%, 57.32% and 73.10%, respectively, in the presence of P. major plus nanoparticles (F-Fe0, Ip-Ag0 and Br-Ag0). The integration of green nanotechnology, solubility-improving agents, and phytoremediation by Plantago major has played a major role in the remediation of soil and water contaminated with chlorfenapyr.

Phytoremediation of azoxystrobin and its degradation products in soil by P. major L. under cold and salinity stress.

Azoxystrobin is a broad-spectrum, systemic and soil-applied fungicide used for crop protection against the four major classes of pathogenic fungi. The use of azoxystrobin use has induced water pollution and ecotoxicological effects upon aquatic organisms, long half-life in soils, as well as heath issues. Such issues may be solved by phytoremediation. Here, we tested the uptake and translocation of azoxystrobin and its degradation products by Plantago major, under cold stress and salt stress. The result demonstrated that azoxystrobin significantly accumulated in P. major roots under salinity conditions more than that in the P. major roots under cold conditions and natural condition within two days of experimental period. In P. major roots and leaves, the chromatograms of HPLC for azoxystrobin and metabolites under natural condition (control) and stressed samples (cold stress and salt stress) show different patterns of metabolism pathways reflecting changes in the degradation products. Azoxystrobin carboxylic acid (AZ-acid) formed by methyl ester hydrolysis was an important route in the roots and the leaves. AZ-pyOH and AZ-benzoic were detected in P. major roots under cold and salt stress, while did not detected in P. major roots under natural condition. In the leaves, AZ-pyOH and AZ-benzoic were detected in all treatments between 4 and 12days of exposure. Shoots of the stressed plants had greater H2O2 and proline contents than was observed in the control plants. The level of 100mM NaCl treatment induced significantly higher peroxidase (POD) activity than the non-treated control group. Leaf Chlorophyll contents in the plants at 80 and 100mM NaCl were significantly reduced than was observed in the control plants. I concluded that P. major had a high potential to contribute to remediation of saline-soil contaminated with azoxystrobin.

Biochemical interactions between Glycine max L. silicon dioxide (SiO2) and plant growth-promoting bacteria (PGPR) for improving phytoremediation of soil contaminated with fenamiphos and its degradation products.

Fenamiphos is a systematic nematicide-insecticide used extensively for the control of soil nematodes. Fenamiphos and oxidation products have been known to induce water pollution, soil pollution and ecotoxicological effects on aquatic organisms, as well as heath issues. This contaminant can be removed by phytoremediation. Herein, we tested several strategies to improve the effectiveness of this technology. A combination of G. max plus Pseudomonas fluorescens was more efficient than G. max plus Serratia marcescens or G. max alone in degrading fenamiphos to other metabolites. Three major metabolites, namely fenamiphos sulfoxide (FSO), fenamiphos sulfone (FSO2) and fenamiphos phenol (F-phenol), were detected in roots and leaves in which G. max amended with P. fluorescens or amended with S. marcescens produced a significant accumulation of FSO and FSO2 with higher amounts than for G. max alone. Leaf concentrations of FSO were always higher than in the roots, while FSO2 accumulated significantly more in G. max roots than in G. max leaves. In soil treated with fenamiphos, G. max roots and leaves alone, and in combined effects of plant and microorganisms, resulted in the disappearance of fenamiphos and the appearance of F-SO, F-SO2 and F-phenol, which in turn caused toxic stress in G. max and the resulting production of reactive oxygen species such as H2O2 with higher content and an increase in antioxidant GPX activity. Although a batch equilibrium technique showed that use of SiO2 resulted in the efficient removal of fenamiphos when compared with other treatments for removing adsorbed fenamiphos from soil, a fewer amount of fenamiphos was removed by G. max L. with SiO2. H2O2 content and GPX activity increased in G. max under fenamiphos treatment and its degradation products, while amended G. max with SiO2 or Argal led to a decrease in GPX activity and H2O2 content.

Potential risks from the accumulation of heavy metals in canola plants.

Concentrations of heavy metals in agricultural land near highways are a major concern for humans. This study was conducted to investigate the contamination level of heavy metals in soil, canola crop, and the potential health risk for honeybee and human. The average concentrations (mg/kg) of Co (15.94), Cr (169.66), Ni (55.39), Mn (765.34) Hg (2.99), and Cu (51.31) were elevated beyond their background reference values in world soil average, while Pb (9.45) was below to their respective background levels. This was confirmed by contamination factor (CF) and ecological risk factors (Er). Heavy metal concentrations in different parts of canola decreased in the following order: Fe> Mn > Cr > Pb > Co > Cu > Ni > Hg. Honey transfer factor (TFH) of heavy metals was less than unity except Ni and Hg. Human health (non-carcinogenic) risk assessment of heavy metals in the soil through potential exposure pathway (ingestion) recorded a dramatically increased risk for children (hazard index, HI=2.44). Hazard quotient via honey (HQH) consumption value of heavy metals were within the safe limits (HQ< 1). Probably, honeybees have a strong ability to transfer Co, Pb, Hg, and Mn (HQ> 1) from the canola to their hives during collecting pollen and nectar. HQ in honeybee workers from the consumption of honey can be used to derive HQ in humans using the hazard factor (HF). HF is 1481.482 (Pb), 2356.902 (Ni), and 3888.889 (Cr), respectively, for adult human (70kg) and 317.460 (Pb), 504.377(Ni), and 832.22 (Cr) for children (15kg).

Phytoremediation of cyanophos insecticide by Plantago major L. in water.

Cyanophos is commonly used in Egypt to control various agricultural and horticultural pests. It is not easily hydrolyzed and thus they are highly persistent and accumulate in various aquatic compartments such as rivers and lakes. Such issues may be solved by phytoremediation, which is the use of plants for the cleanup of pollutants. Here, we tested Plantago major L. to clean water polluted with cyanophos insecticide under laboratory conditions.The biosorption capacity (KF) of cyanophos were 76.91, 26.18 and 21.09 µg/g for dry roots, fruit (seeds with shells) and leaves of the Plantago major L., respectively. Viable Plantago major L. in water significantly reduced cyanophos by 11.0% & 94.7% during 2 hours & 9 days of exposure as compared with 0.8% & 36.9% in water without the plantain. In water with plantain, cyanophos significantly accumulated in plantain roots and leaves to reach maximum levels after two and four hours of treatment, respectively. After 1 day, the concentration of cyanophos decreased in roots and shoots until the end of testing. Three major degradation products were detected at roots and leaf samples. Here we demonstrate that plantago major L. removes efficiently cyanophos residue in water and has a potential activity for pesticide phytoremediation.