RESUMEN
The proportion of activated persulfate (PS) oxidation technology in the remediation of domestic organic contaminated sites has increased every year, and the potential corrosion risk of site reuse caused by residual oxidants and by-products has also attracted the attention of researchers. In this work, the potential corrosion degree such as the mass reduction rate and surface crack width of standard iron flakes under different conditions, including with different PS dosages and release times, was monitored quantitatively over a long period, and the corresponding corrosion risk was quantitatively assessed. The results showed that when n (Na2S2O8):n (PAHs) increased from 5:1 to 100:1, the higher the oxidizer dosage, the more severe the corrosion weight loss and surface crack width, indicating that the oxidizer dosage was positively correlated with the potential corrosion risk. In addition, the corrosion crack width of the standard iron flake had a significant positive correlation with the reaction time and a significant negative correlation with the mass change. According to the changes in the standard iron flake, the corrosion process could be divided into three stages, in which the corrosion risk from high to low followed the order of oxidant corrosion stageâ¯>â¯oxidant and salt corrosion stageâ¯>â¯salt and microbial corrosion stage. Therefore, the dosage of chemicals should be controlled, the molar ratio of oxidizer to contaminant should not exceed 25:1, and a natural recovery period of at least one year should be left post remediation. During the reuse of the remediation sites in the future, the potential corrosion risks should also be calculated based on the dosage and time, to avoid redevelopment and use of the restoration site in the high corrosion risk stage.
Asunto(s)
Restauración y Remediación Ambiental , Contaminantes del Suelo , Contaminantes del Suelo/análisis , Corrosión , Oxidación-Reducción , Hierro , Oxidantes , Suelo , SulfatosRESUMEN
Foxp3(+) regulatory T cells (Treg) playing a crucial role in the maintenance of immune tolerance and prevention of autoimmune diseases consist of thymus-derived naturally occurring CD4(+)Foxp3(+) Treg cells (nTreg) and those that can be induced ex vivo with TGF-ß (iTreg). Although both Treg subsets share similar phenotypes and functional characteristics, they also have potential biologic differences on their biology. The role of iTreg in regulating B cells remains unclear so far. The suppression assays of Treg subsets on activation, proliferation, and Abs production of B cells were measured using a Treg and B cell coculture system in vitro. Transwell and Ab blockade experiments were performed to assess the roles of cell contact and soluble cytokines. Treg were adoptively transferred to lupus mice to assess in vivo effects on B cells. Like nTreg, iTreg subset also directly suppressed activation and proliferation of B cells. nTreg subset suppressed B cell responses through cytotoxic manner related to expression of granzyme A, granzyme B, and perforin, whereas the role of iTreg subset on B cells did not involve in cytotoxic action but depending on TGF-ß signaling. Furthermore, iTreg subset can significantly suppress Ab produced by lupus B cells in vitro. Comparison experiments using autoantibodies microarrays demonstrated that adoptive transfer of iTreg had a superior effect than nTreg subset on suppressing lupus B cell responses in vivo. Our data implicate a role and advantage of iTreg subset in treating B cell-mediated autoimmune diseases, boosting the translational potential of these findings.
Asunto(s)
Linfocitos B/inmunología , Lupus Eritematoso Sistémico/inmunología , Linfocitos T Reguladores/inmunología , Factor de Crecimiento Transformador beta/inmunología , Traslado Adoptivo , Animales , Autoanticuerpos/biosíntesis , Linfocitos B/efectos de los fármacos , Linfocitos B/fisiología , Diferenciación Celular/efectos de los fármacos , Diferenciación Celular/inmunología , Proliferación Celular/efectos de los fármacos , Técnicas de Cocultivo , Granzimas/deficiencia , Granzimas/genética , Granzimas/metabolismo , Tolerancia Inmunológica , Interleucina-2/farmacología , Activación de Linfocitos/efectos de los fármacos , Ratones , Análisis de Secuencia por Matrices de Oligonucleótidos , Linfocitos T Reguladores/efectos de los fármacos , Linfocitos T Reguladores/fisiología , Factor de Crecimiento Transformador beta/farmacologíaRESUMEN
A life cycle conceptual site model (LC-CSM) can represent the actual spatial distribution and migration of pollution of a site very accurately and be beneficial in supporting decisions for accurate site remediation or risk management. A volatile chlorinated hydrocarbon contaminated site in the Beijing-Tianjin-Hebei region was chosen as the study case. LC-CSMs were established following the site assessment, preliminary investigation, detailed investigation, and supplementary investigation of each stage. The application of field screening tests such as a membrane interface probe and the multi-electrode resistivity method assisted in identifying potential pollution sources and hot points. Concurrently, a large amount of vinyl chloride, the end product of chlorinated hydrocarbon degradation, was detected in some boreholes, indicating that pollutant biodegradation had occurred at this site. Some typical boreholes and cross-sections were chosen to analyze the biodegradation indicators and chemical fingerprints, combining the results of the comprehensive score of chlorinated hydrocarbon anaerobic biodegradability in groundwater reaching 22. It is judged that the site has strong anaerobic biodegradability. This step-by-step optimization forms an LC-CSM for site investigation, which provides scientific support for accurate site characterization.