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Eur Respir J ; 49(5)2017 05.
Artículo en Inglés | MEDLINE | ID: mdl-28495692

RESUMEN

Despite its high prevalence and mortality, little is known about the pathogenesis of rheumatoid arthritis-associated interstitial lung disease (RA-ILD). Given that familial pulmonary fibrosis (FPF) and RA-ILD frequently share the usual pattern of interstitial pneumonia and common environmental risk factors, we hypothesised that the two diseases might share additional risk factors, including FPF-linked genes. Our aim was to identify coding mutations of FPF-risk genes associated with RA-ILD.We used whole exome sequencing (WES), followed by restricted analysis of a discrete number of FPF-linked genes and performed a burden test to assess the excess number of mutations in RA-ILD patients compared to controls.Among the 101 RA-ILD patients included, 12 (11.9%) had 13 WES-identified heterozygous mutations in the TERT, RTEL1, PARN or SFTPC coding regions. The burden test, based on 81 RA-ILD patients and 1010 controls of European ancestry, revealed an excess of TERT, RTEL1, PARN or SFTPC mutations in RA-ILD patients (OR 3.17, 95% CI 1.53-6.12; p=9.45×10-4). Telomeres were shorter in RA-ILD patients with a TERT, RTEL1 or PARN mutation than in controls (p=2.87×10-2).Our results support the contribution of FPF-linked genes to RA-ILD susceptibility.


Asunto(s)
Artritis Reumatoide/genética , Predisposición Genética a la Enfermedad , Enfermedades Pulmonares Intersticiales/genética , Fibrosis Pulmonar/genética , Adulto , Anciano , Artritis Reumatoide/complicaciones , Estudios de Casos y Controles , ADN Helicasas/genética , Europa (Continente) , Exoma , Femenino , Estudios de Asociación Genética , Heterocigoto , Humanos , Enfermedades Pulmonares Intersticiales/complicaciones , Masculino , Persona de Mediana Edad , Mutación , Fenotipo , Fibrosis Pulmonar/complicaciones , Factores de Riesgo , Análisis de Secuencia de ADN , Programas Informáticos , Telomerasa/genética
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