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1.
Nature ; 629(8012): 652-659, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38693261

RESUMEN

The gut microbiota operates at the interface of host-environment interactions to influence human homoeostasis and metabolic networks1-4. Environmental factors that unbalance gut microbial ecosystems can therefore shape physiological and disease-associated responses across somatic tissues5-9. However, the systemic impact of the gut microbiome on the germline-and consequently on the F1 offspring it gives rise to-is unexplored10. Here we show that the gut microbiota act as a key interface between paternal preconception environment and intergenerational health in mice. Perturbations to the gut microbiota of prospective fathers increase the probability of their offspring presenting with low birth weight, severe growth restriction and premature mortality. Transmission of disease risk occurs via the germline and is provoked by pervasive gut microbiome perturbations, including non-absorbable antibiotics or osmotic laxatives, but is rescued by restoring the paternal microbiota before conception. This effect is linked with a dynamic response to induced dysbiosis in the male reproductive system, including impaired leptin signalling, altered testicular metabolite profiles and remapped small RNA payloads in sperm. As a result, dysbiotic fathers trigger an elevated risk of in utero placental insufficiency, revealing a placental origin of mammalian intergenerational effects. Our study defines a regulatory 'gut-germline axis' in males, which is sensitive to environmental exposures and programmes offspring fitness through impacting placenta function.


Asunto(s)
Susceptibilidad a Enfermedades , Disbiosis , Padre , Microbioma Gastrointestinal , Insuficiencia Placentaria , Lesiones Prenatales , Espermatozoides , Animales , Femenino , Masculino , Ratones , Embarazo , Disbiosis/complicaciones , Disbiosis/microbiología , Microbioma Gastrointestinal/fisiología , Leptina/metabolismo , Ratones Endogámicos C57BL , Placenta/metabolismo , Placenta/fisiopatología , Insuficiencia Placentaria/etiología , Insuficiencia Placentaria/metabolismo , Insuficiencia Placentaria/fisiopatología , Resultado del Embarazo , Lesiones Prenatales/etiología , Lesiones Prenatales/metabolismo , Lesiones Prenatales/fisiopatología , Transducción de Señal , Espermatozoides/metabolismo , Testículo/metabolismo , Testículo/fisiopatología , Susceptibilidad a Enfermedades/etiología
2.
Cell ; 150(1): 88-99, 2012 Jul 06.
Artículo en Inglés | MEDLINE | ID: mdl-22738725

RESUMEN

Transgenerational effects have wide-ranging implications for human health, biological adaptation, and evolution; however, their mechanisms and biology remain poorly understood. Here, we demonstrate that a germline nuclear small RNA/chromatin pathway can maintain stable inheritance for many generations when triggered by a piRNA-dependent foreign RNA response in C. elegans. Using forward genetic screens and candidate approaches, we find that a core set of nuclear RNAi and chromatin factors is required for multigenerational inheritance of environmental RNAi and piRNA silencing. These include a germline-specific nuclear Argonaute HRDE1/WAGO-9, a HP1 ortholog HPL-2, and two putative histone methyltransferases, SET-25 and SET-32. piRNAs can trigger highly stable long-term silencing lasting at least 20 generations. Once established, this long-term memory becomes independent of the piRNA trigger but remains dependent on the nuclear RNAi/chromatin pathway. Our data present a multigenerational epigenetic inheritance mechanism induced by piRNAs.


Asunto(s)
Caenorhabditis elegans/genética , Epigenómica , Interferencia de ARN , ARN de Helminto/metabolismo , ARN Interferente Pequeño/metabolismo , Animales , Caenorhabditis elegans/metabolismo , Núcleo Celular/genética , Núcleo Celular/metabolismo , Femenino , Células Germinativas/metabolismo , Masculino , Transgenes
3.
Genes Dev ; 33(13-14): 857-870, 2019 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-31147388

RESUMEN

Piwi proteins are important for germ cell development in most animals. These proteins are guided to specific targets by small guide RNAs, referred to as piRNAs or 21U RNAs in Caenorhabditis elegans In this organism, even though genetic screens have uncovered 21U RNA biogenesis factors, little is known about how these factors interact or what they do. Based on the previously identified 21U biogenesis factor PID-1 (piRNA-induced silencing-defective 1), we here define a novel protein complex, PETISCO (PID-3, ERH-2, TOFU-6, and IFE-3 small RNA complex), that is required for 21U RNA biogenesis. PETISCO contains both potential 5' cap and 5' phosphate RNA-binding domains and interacts with capped 21U precursor RNA. We resolved the architecture of PETISCO and revealed a second function for PETISCO in embryonic development. This essential function of PETISCO is mediated not by PID-1 but by the novel protein TOST-1 (twenty-one U pathway antagonist). In contrast, TOST-1 is not essential for 21U RNA biogenesis. Both PID-1 and TOST-1 interact directly with ERH-2 using a conserved sequence motif. Finally, our data suggest a role for TOST-1:PETISCO in SL1 homeostasis in the early embryo. Our work describes a key complex for 21U RNA processing in C. elegans and strengthens the view that 21U RNA biogenesis is built on an snRNA-related pathway.


Asunto(s)
Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Embrión no Mamífero/fisiología , Complejos Multiproteicos/genética , Complejos Multiproteicos/metabolismo , ARN Nucleolar Pequeño/biosíntesis , Animales , ARN Nuclear Pequeño/metabolismo
4.
PLoS Biol ; 21(8): e3002279, 2023 08.
Artículo en Inglés | MEDLINE | ID: mdl-37585418

RESUMEN

Cells from most eukaryotic species make several different types of small interfering RNAs. Pioneering work in plants, published in PLOS Biology almost 20 years ago, established a framework to understand how multiple RNA interference pathways can regulate the genome in parallel.


Asunto(s)
Plantas , Semillas , Interferencia de ARN , ARN Interferente Pequeño/genética , ARN Interferente Pequeño/metabolismo , Plantas/genética , Plantas/metabolismo , Semillas/genética , Semillas/metabolismo , Bosques
5.
PLoS Biol ; 21(10): e3002354, 2023 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-37883365

RESUMEN

The N-terminal tails of eukaryotic histones are frequently posttranslationally modified. The role of these modifications in transcriptional regulation is well-documented. However, the extent to which the enzymatic processes of histone posttranslational modification might affect metabolic regulation is less clear. Here, we investigated how histone methylation might affect metabolism using metabolomics, proteomics, and RNA-seq data from cancer cell lines, primary tumour samples and healthy tissue samples. In cancer, the expression of histone methyltransferases (HMTs) was inversely correlated to the activity of NNMT, an enzyme previously characterised as a methyl sink that disposes of excess methyl groups carried by the universal methyl donor S-adenosyl methionine (SAM or AdoMet). In healthy tissues, histone methylation was inversely correlated to the levels of an alternative methyl sink, PEMT. These associations affected the levels of multiple histone marks on chromatin genome-wide but had no detectable impact on transcriptional regulation. We show that HMTs with a variety of different associations to transcription are co-regulated by the Retinoblastoma (Rb) tumour suppressor in human cells. Rb-mutant cancers show increased total HMT activity and down-regulation of NNMT. Together, our results suggest that the total activity of HMTs affects SAM metabolism, independent of transcriptional regulation.


Asunto(s)
Histonas , Neoplasias , Humanos , Metilación , Histonas/metabolismo , N-Metiltransferasa de Histona-Lisina/genética , N-Metiltransferasa de Histona-Lisina/metabolismo , Histona Metiltransferasas/metabolismo , S-Adenosilmetionina/metabolismo , Procesamiento Proteico-Postraduccional , Neoplasias/genética
6.
Nat Rev Mol Cell Biol ; 15(8): 525-35, 2014 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-25053358

RESUMEN

Small RNAs that function in a non-cell autonomous manner are becoming increasingly recognized as regulatory molecules with the potential to transmit information between cells, organisms and species. In plants and nematodes, small RNA mobility can be genetically dissected to provide information about the nature of the mobile RNA species, their distribution in the organism and inside cells, as well as the cellular machinery required for mobility, including channel proteins and cellular trafficking factors. Mobile RNAs function in antiviral defence, cell signalling and gene expression regulation, and might also mediate transgenerational epigenetic inheritance.


Asunto(s)
ARN Pequeño no Traducido/metabolismo , Animales , Transporte Biológico , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Epigénesis Genética , Humanos , Inmunidad/genética , Plantas/genética , Plantas/metabolismo , ARN Interferente Pequeño , ARN Pequeño no Traducido/genética , Transducción de Señal/genética , Virosis/genética , Virosis/inmunología
7.
PLoS Genet ; 19(3): e1010647, 2023 03.
Artículo en Inglés | MEDLINE | ID: mdl-36862744

RESUMEN

Some epigenetic information can be transmitted between generations without changes in the underlying DNA sequence. Changes in epigenetic regulators, termed epimutations, can occur spontaneously and be propagated in populations in a manner reminiscent of DNA mutations. Small RNA-based epimutations occur in C. elegans and persist for around 3-5 generations on average. Here, we explored whether chromatin states also undergo spontaneous change and whether this could be a potential alternative mechanism for transgenerational inheritance of gene expression changes. We compared the chromatin and gene expression profiles at matched time points from three independent lineages of C. elegans propagated at minimal population size. Spontaneous changes in chromatin occurred in around 1% of regulatory regions each generation. Some were heritable epimutations and were significantly enriched for heritable changes in expression of nearby protein-coding genes. Most chromatin-based epimutations were short-lived but a subset had longer duration. Genes subject to long-lived epimutations were enriched for multiple components of xenobiotic response pathways. This points to a possible role for epimutations in adaptation to environmental stressors.


Asunto(s)
Cromatina , Epigénesis Genética , Animales , Cromatina/genética , Metilación de ADN , Caenorhabditis elegans/genética , Expresión Génica
8.
Proc Natl Acad Sci U S A ; 120(5): e2217992120, 2023 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-36689659

RESUMEN

SWItch/sucrose non-fermenting (SWI/SNF) complexes are a family of chromatin remodelers that are conserved across eukaryotes. Mutations in subunits of SWI/SNF cause a multitude of different developmental disorders in humans, most of which have no current treatment options. Here, we identify an alanine-to-valine-causing mutation in the SWI/SNF subunit snfc-5 (SMARCB1 in humans) that prevents embryonic lethality in Caenorhabditis elegans nematodes harboring a loss-of-function mutation in the SWI/SNF subunit swsn-1 (SMARCC1/2 in humans). Furthermore, we found that the combination of this specific mutation in snfc-5 and a loss-of-function mutation in either of the E3 ubiquitin ligases ubr-5 (UBR5 in humans) or hecd-1 (HECTD1 in humans) can restore development to adulthood in swsn-1 loss-of-function mutants that otherwise die as embryos. Using these mutant models, we established a set of 335 genes that are dysregulated in SWI/SNF mutants that arrest their development embryonically but exhibit near wild-type levels of expression in the presence of suppressor mutations that prevent embryonic lethality, suggesting that SWI/SNF promotes development by regulating some subset of these 335 genes. In addition, we show that SWI/SNF protein levels are reduced in swsn-1; snfc-5 double mutants and partly restored to wild-type levels in swsn-1; snfc-5; ubr-5 triple mutants, consistent with a model in which UBR-5 regulates SWI/SNF levels by tagging the complex for proteasomal degradation. Our findings establish a link between two E3 ubiquitin ligases and SWI/SNF function and suggest that UBR5 and HECTD1 could be potential therapeutic targets for the many developmental disorders caused by missense mutations in SWI/SNF subunits.


Asunto(s)
Proteínas de Caenorhabditis elegans , Caenorhabditis elegans , Animales , Humanos , Caenorhabditis elegans/metabolismo , Ubiquitina-Proteína Ligasas/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Ubiquitinas/metabolismo
9.
EMBO J ; 40(5): e105564, 2021 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-33340372

RESUMEN

Piwi-interacting RNAs (piRNAs) play key roles in germline development and genome defence in metazoans. In C. elegans, piRNAs are transcribed from > 15,000 discrete genomic loci by RNA polymerase II (Pol II), resulting in 28 nt short-capped piRNA precursors. Here, we investigate transcription termination at piRNA loci. We show that the Integrator complex, which terminates snRNA transcription, is recruited to piRNA loci. Moreover, we demonstrate that the catalytic activity of Integrator cleaves nascent capped piRNA precursors associated with promoter-proximal Pol II, resulting in termination of transcription. Loss of Integrator activity, however, does not result in transcriptional readthrough at the majority of piRNA loci. Taken together, our results draw new parallels between snRNA and piRNA biogenesis in nematodes and provide evidence of a role for the Integrator complex as a terminator of promoter-proximal RNA polymerase II during piRNA biogenesis.


Asunto(s)
Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/metabolismo , Regiones Promotoras Genéticas , ARN Polimerasa II/metabolismo , Precursores del ARN/metabolismo , Procesamiento Postranscripcional del ARN , ARN Interferente Pequeño/metabolismo , Animales , Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/genética , Células Germinativas , Caperuzas de ARN , ARN Polimerasa II/genética , Precursores del ARN/genética , ARN Interferente Pequeño/genética , Transcripción Genética
10.
EMBO J ; 40(5): e105565, 2021 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-33533030

RESUMEN

PIWI-interacting RNAs (piRNAs) are genome-encoded small RNAs that regulate germ cell development and maintain germline integrity in many animals. Mature piRNAs engage Piwi Argonaute proteins to silence complementary transcripts, including transposable elements and endogenous genes. piRNA biogenesis mechanisms are diverse and remain poorly understood. Here, we identify the RNA polymerase II (RNA Pol II) core subunit RPB-9 as required for piRNA-mediated silencing in the nematode Caenorhabditis elegans. We show that rpb-9 initiates heritable piRNA-mediated gene silencing at two DNA transposon families and at a subset of somatic genes in the germline. We provide genetic and biochemical evidence that RPB-9 is required for piRNA biogenesis by recruiting the Integrator complex at piRNA genes, hence promoting transcriptional termination. We conclude that, as a part of its rapid evolution, the piRNA pathway has co-opted an ancient machinery for high-fidelity transcription.


Asunto(s)
Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/metabolismo , Regulación de la Expresión Génica , Silenciador del Gen , ARN Polimerasa II/metabolismo , ARN Interferente Pequeño/metabolismo , Transcripción Genética , Animales , Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/genética , Células Germinativas , Regiones Promotoras Genéticas , Subunidades de Proteína , ARN Polimerasa II/genética , ARN Interferente Pequeño/genética
11.
Genome Res ; 32(11-12): 2015-2027, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36351773

RESUMEN

Different modes of reproduction evolve rapidly, with important consequences for genome composition. Selfing species often occupy a similar niche as their outcrossing sister species with which they are able to mate and produce viable hybrid progeny, raising the question of how they maintain genomic identity. Here, we investigate this issue by using the nematode Caenorhabditis briggsae, which reproduces as a hermaphrodite, and its outcrossing sister species Caenorhabditis nigoni We hypothesize that selfing species might develop some barriers to prevent gene intrusions through gene regulation. We therefore examined gene regulation in the hybrid F2 embryos resulting from reciprocal backcrosses between F1 hybrid progeny and C. nigoni or C. briggsae F2 hybrid embryos with ∼75% of their genome derived from C. briggsae (termed as bB2) were inviable, whereas those with ∼75% of their genome derived from C. nigoni (termed as nB2) were viable. Misregulation of transposable elements, coding genes, and small regulatory RNAs was more widespread in the bB2 compared with the nB2 hybrids, which is a plausible explanation for the differential phenotypes between the two hybrids. Our results show that regulation of the C. briggsae genome is strongly affected by genetic exchanges with its outcrossing sister species, C. nigoni, whereas regulation of the C. nigoni genome is more robust on genetic exchange with C. briggsae The results provide new insights into how selfing species might maintain their identity despite genetic exchanges with closely related outcrossing species.


Asunto(s)
Caenorhabditis , Animales , Caenorhabditis/genética , Genoma , Reproducción/genética , Fenotipo
12.
BMC Biol ; 21(1): 276, 2023 11 29.
Artículo en Inglés | MEDLINE | ID: mdl-38031056

RESUMEN

BACKGROUND: The individual lifestyle and environment of an organism can influence its phenotype and potentially the phenotype of its offspring. The different genetic and non-genetic components of the inheritance system and their mutual interactions are key mechanisms to generate inherited phenotypic changes. Epigenetic changes can be transmitted between generations independently from changes in DNA sequence. In Caenorhabditis elegans, epigenetic differences, i.e. epimutations, mediated by small non-coding RNAs, particularly 22G-RNAs, as well as chromatin have been identified, and their average persistence is three to five generations. In addition, previous research showed that some epimutations had a longer duration and concerned genes that were enriched for multiple components of xenobiotic response pathways. These results raise the possibility that environmental stresses might change the rate at which epimutations occur, with potential significance for adaptation. RESULTS: In this work, we explore this question by propagating C. elegans lines either in control conditions or in moderate or high doses of cisplatin, which introduces genotoxic stress by damaging DNA. Our results show that cisplatin has a limited effect on global small non-coding RNA epimutations and epimutations in gene expression levels. However, cisplatin exposure leads to increased fluctuations in the levels of small non-coding RNAs derived from tRNA cleavage. We show that changes in tRNA-derived small RNAs may be associated with gene expression changes. CONCLUSIONS: Our work shows that epimutations are not substantially altered by cisplatin exposure but identifies transient changes in tRNA-derived small RNAs as a potential source of variation induced by genotoxic stress.


Asunto(s)
Caenorhabditis elegans , Metilación de ADN , Animales , Caenorhabditis elegans/genética , Cisplatino/toxicidad , Mutación , Epigénesis Genética , ARN , ARN de Transferencia/genética
13.
PLoS Pathog ; 17(1): e1009286, 2021 01.
Artículo en Inglés | MEDLINE | ID: mdl-33497411

RESUMEN

Animal-parasitic nematodes have thus far been largely refractory to genetic manipulation, and methods employed to effect RNA interference (RNAi) have been ineffective or inconsistent in most cases. We describe here a new approach for genetic manipulation of Nippostrongylus brasiliensis, a widely used laboratory model of gastrointestinal nematode infection. N. brasiliensis was successfully transduced with Vesicular Stomatitis Virus glycoprotein G (VSV-G)-pseudotyped lentivirus. The virus was taken up via the nematode intestine, RNA reverse transcribed into proviral DNA, and transgene transcripts produced stably in infective larvae, which resulted in expression of the reporter protein mCherry. Improved transgene expression was achieved by incorporating the C. elegans hlh11 promoter and the tbb2 3´-UTR into viral constructs. MicroRNA-adapted short hairpin RNAs delivered in this manner were processed correctly and resulted in partial knockdown of ß-tubulin isotype-1 (tbb-iso-1) and secreted acetylcholinesterase B (ache-B). The system was further refined by lentiviral delivery of double stranded RNAs, which acted as a trigger for RNAi following processing and generation of 22G-RNAs. Virus-encoded sequences were detectable in F1 eggs and third stage larvae, demonstrating that proviral DNA entered the germline and was heritable. Lentiviral transduction thus provides a new means for genetic manipulation of parasitic nematodes, including gene silencing and expression of exogenous genes.


Asunto(s)
Lentivirus/genética , Nippostrongylus/virología , ARN Interferente Pequeño/genética , Infecciones por Strongylida/parasitología , Acetilcolinesterasa/genética , Acetilcolinesterasa/metabolismo , Animales , Caenorhabditis elegans/genética , Caenorhabditis elegans/virología , Femenino , Silenciador del Gen , Larva , Lentivirus/fisiología , Masculino , Nippostrongylus/genética , Interferencia de ARN , ARN Bicatenario/genética , Ratas , Ratas Sprague-Dawley , Transducción Genética
14.
PLoS Genet ; 16(6): e1008864, 2020 06.
Artículo en Inglés | MEDLINE | ID: mdl-32584820

RESUMEN

Cytosine methylation is an ancient epigenetic modification yet its function and extent within genomes is highly variable across eukaryotes. In mammals, methylation controls transposable elements and regulates the promoters of genes. In insects, DNA methylation is generally restricted to a small subset of transcribed genes, with both intergenic regions and transposable elements (TEs) depleted of methylation. The evolutionary origin and the function of these methylation patterns are poorly understood. Here we characterise the evolution of DNA methylation across the arthropod phylum. While the common ancestor of the arthropods had low levels of TE methylation and did not methylate promoters, both of these functions have evolved independently in centipedes and mealybugs. In contrast, methylation of the exons of a subset of transcribed genes is ancestral and widely conserved across the phylum, but has been lost in specific lineages. A similar set of genes is methylated in all species that retained exon-enriched methylation. We show that these genes have characteristic patterns of expression correlating to broad transcription initiation sites and well-positioned nucleosomes, providing new insights into potential mechanisms driving methylation patterns over hundreds of millions of years.


Asunto(s)
Artrópodos/genética , Metilación de ADN , Epigénesis Genética , Evolución Molecular , Animales , Islas de CpG/genética , Elementos Transponibles de ADN/genética , Exones/genética , Filogenia , Regiones Promotoras Genéticas/genética
15.
Semin Cell Dev Biol ; 97: 106-115, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31228598

RESUMEN

Recently interest in multi-generational epigenetic phenomena have been fuelled by highly reproducible intergenerational and transgenerational inheritance paradigms in several model organisms. Such paradigms are essential in order to begin to use genetics to unpick the mechanistic bases of how epigenetic information may be transmitted between generations; indeed great strides have been made towards understanding these mechanisms. Far less well understood is the relationship between epigenetic inheritance, ecology and evolution. In this review I focus on potential connections between laboratory studies of transgenerational epigenetic inheritance phenomena and evolutionary processes that occur in natural populations. In the first section, I consider whether transgenerational epigenetic inheritance might provide an advantage to organisms over the short term in adapting to their environment. Second, I consider whether epigenetic changes can contribute to the evolution of species by contributing to stable phenotypic variation within a population. Finally I discuss whether epigenetic changes could influence evolution by either directly or indirectly promoting DNA sequence changes that could impact phenotypic divergence. Additionally, I will discuss how epigenetic changes could influence the evolution of human cancer and thus be directly relevant for the development of this disease.


Asunto(s)
Epigénesis Genética/genética , Patrón de Herencia , Humanos
16.
Gastroenterology ; 160(1): 232-244.e7, 2021 01.
Artículo en Inglés | MEDLINE | ID: mdl-32814113

RESUMEN

BACKGROUND & AIMS: Gene expression patterns of CD8+ T cells have been reported to correlate with clinical outcomes of adults with inflammatory bowel diseases (IBD). We aimed to validate these findings in independent patient cohorts. METHODS: We obtained peripheral blood samples from 112 children with a new diagnosis of IBD (71 with Crohn's disease and 41 with ulcerative colitis) and 19 children without IBD (controls) and recorded medical information on disease activity and outcomes. CD8+ T cells were isolated from blood samples by magnetic bead sorting at the point of diagnosis and during the course of disease. Genome-wide transcription (n = 192) and DNA methylation (n = 66) profiles were generated using Affymetrix and Illumina arrays, respectively. Publicly available transcriptomes and DNA methylomes of CD8+ T cells from 3 adult patient cohorts with and without IBD were included in data analyses. RESULTS: Previously reported CD8+ T-cell prognostic expression and exhaustion signatures were only found in the original adult IBD patient cohort. These signatures could not be detected in either a pediatric or a second adult IBD cohort. In contrast, an association between CD8+ T-cell gene expression with age and sex was detected across all 3 cohorts. CD8+ gene transcription was clearly associated with IBD in the 2 cohorts that included non-IBD controls. Lastly, DNA methylation profiles of CD8+ T cells from children with Crohn's disease correlated with age but not with disease outcome. CONCLUSIONS: We were unable to validate previously reported findings of an association between CD8+ T-cell gene transcription and disease outcome in IBD. Our findings reveal the challenges of developing prognostic biomarkers for patients with IBD and the importance of their validation in large, independent cohorts before clinical application.


Asunto(s)
Linfocitos T CD8-positivos/fisiología , Enfermedades Inflamatorias del Intestino/diagnóstico , Enfermedades Inflamatorias del Intestino/etiología , Adolescente , Adulto , Factores de Edad , Estudios de Casos y Controles , Niño , Preescolar , Metilación de ADN , Femenino , Humanos , Masculino , Valor Predictivo de las Pruebas , Pronóstico , Transcripción Genética , Adulto Joven
17.
Development ; 146(19)2019 09 26.
Artículo en Inglés | MEDLINE | ID: mdl-31558570

RESUMEN

Over the past few years, interest in chromatin and its evolution has grown. To further advance these interests, we organized a workshop with the support of The Company of Biologists to debate the current state of knowledge regarding the origin and evolution of chromatin. This workshop led to prospective views on the development of a new field of research that we term 'EvoChromo'. In this short Spotlight article, we define the breadth and expected impact of this new area of scientific inquiry on our understanding of both chromatin and evolution.


Asunto(s)
Cromatina/genética , Evolución Molecular , Animales , Genoma , Humanos
18.
Biochem Soc Trans ; 50(3): 1179-1190, 2022 06 30.
Artículo en Inglés | MEDLINE | ID: mdl-35521905

RESUMEN

DNA methylation is an epigenetic modification with a very long evolutionary history. However, DNA methylation evolves surprisingly rapidly across eukaryotes. The genome-wide distribution of methylation diversifies rapidly in different lineages, and DNA methylation is lost altogether surprisingly frequently. The growing availability of genomic and epigenomic sequencing across organisms highlights this diversity but also illuminates potential factors that could explain why both the DNA methylation machinery and its genome-wide distribution evolve so rapidly. Key to this are new discoveries about the fitness costs associated with DNA methylation, and new theories about how the fundamental biochemical mechanisms of DNA methylation introduction and maintenance could explain how new genome-wide patterns of methylation evolve.


Asunto(s)
Metilación de ADN , Eucariontes , Epigénesis Genética , Epigenómica , Eucariontes/genética , Células Eucariotas
19.
Mol Syst Biol ; 17(6): e9600, 2021 06.
Artículo en Inglés | MEDLINE | ID: mdl-34169647

RESUMEN

Transposable elements (TEs) are widespread across eukaryotic genomes, yet their content varies widely between different species. Factors shaping the diversity of TEs are poorly understood. Understanding the evolution of TEs is difficult because their sequences diversify rapidly and TEs are often transferred through non-conventional means such as horizontal gene transfer. We developed a method to track TE evolution using network analysis to visualise TE sequence and TE content across different genomes. We illustrate our method by first using a monopartite network to study the sequence evolution of Tc1/mariner elements across focal species. We identify a connection between two subfamilies associated with convergent acquisition of a domain from a protein-coding gene. Second, we use a bipartite network to study how TE content across species is shaped by epigenetic silencing mechanisms. We show that the presence of Piwi-interacting RNAs is associated with differences in network topology after controlling for phylogenetic effects. Together, our method demonstrates how a network-based approach can identify hitherto unknown properties of TE evolution across species.


Asunto(s)
Elementos Transponibles de ADN , Evolución Molecular , Elementos Transponibles de ADN/genética , Filogenia , ARN Interferente Pequeño
20.
Adv Exp Med Biol ; 1389: 349-361, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36350517

RESUMEN

Ever since the discovery of depletion of CG sites in mammalian genomes it has been clear that cytosine DNA methyltransferases (DNMTs) are linked to the rate at which mutations accumulate in DNA. Research in the intervening decades has shown that DNMTs influence mutation rates through the indirect consequences of methylation on the mechanism of mutation and the mechanisms for DNA repair. Additionally, recent studies have shown that DNA methyltransferases have the potential to directly introduce damage into DNA. Here, I will discuss both aspects of the connection between DNMTs and DNA damage, evaluating the potential consequences for evolution across species and in human diseases such as cancer where cellular evolution plays a key role.


Asunto(s)
ADN (Citosina-5-)-Metiltransferasas , Metilación de ADN , Animales , Humanos , ADN (Citosina-5-)-Metiltransferasas/genética , Metilasas de Modificación del ADN/genética , Metilasas de Modificación del ADN/metabolismo , Daño del ADN/genética , Mamíferos/genética , ADN/genética
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